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Protein

Probable glutathione-independent glyoxalase SNO4

Gene

SNO4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of methylglyoxal (MG) to D-lactate in a single glutathione (GSH)-independent step. May play a role in detoxifying endogenously produced glyoxals. Involved in protection against reactive oxygen species (ROS) (By similarity). Important for viability in stationary phase. May negatively regulate TORC1 in response to nutrient limitation (PubMed:24706893).By similarity1 Publication

Catalytic activityi

(R)-lactate = methylglyoxal + H2O.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei138 – 1381By similarity
Active sitei139 – 1391By similarity
Active sitei170 – 1701By similarity

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: SGD

GO - Biological processi

  1. cellular response to nutrient levels Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciYEAST:G3O-32985-MONOMER.

Protein family/group databases

MEROPSiC56.A04.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutathione-independent glyoxalase SNO4By similarity (EC:4.2.1.130By similarity)
Alternative name(s):
Glyoxalase 3 homolog 4By similarity
Heat shock protein 341 Publication
SNZ proximal open reading frame 41 Publication
Gene namesi
Name:SNO41 Publication
Synonyms:HSP341 Publication
Ordered Locus Names:YMR322CImported
ORF Names:YM9924.14C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XIII

Organism-specific databases

CYGDiYMR322c.
SGDiS000004941. SNO4.

Subcellular locationi

CytoplasmP-body By similarity
Note: Present in processing bodies (P-bodies) and stress granule (SG) foci upon glucose starvation and heat shock.By similarity

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Results in higher sensitivity to oxidative stress, reduced thermotolerance, accumulation of higher levels of reactive oxygen species, and reduced chronological life span.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 237237Probable glutathione-independent glyoxalase SNO4PRO_0000157853Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriQ04902.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi35501. 57 interactions.
DIPiDIP-1968N.
IntActiQ04902. 8 interactions.
MINTiMINT-386922.
STRINGi4932.YMR322C.

Structurei

3D structure databases

ProteinModelPortaliQ04902.
SMRiQ04902. Positions 4-237.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000013431.
HOGENOMiHOG000181653.
InParanoidiQ04902.
OrthoDBiEOG73547H.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR002818. ThiJ/PfpI.
[Graphical view]
PfamiPF01965. DJ-1_PfpI. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.

Sequencei

Sequence statusi: Complete.

Q04902-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPKRALISL TSYHGPFYKD GAKTGVFVVE ILRSFDTFEK HGFEVDFVSE
60 70 80 90 100
TGGFGWDEHY LPKSFIGGED KMNFETKNSA FNKALARIKT ANEVNASDYK
110 120 130 140 150
IFFASAGHGA LFDYPKAKNL QDIASKIYAN GGVIAAICHG PLLFDGLIDI
160 170 180 190 200
KTTRPLIEGK AITGFPLEGE IALGVDDILR SRKLTTVERV ANKNRAKYLA
210 220 230
PIHPWDDYSI TDGKLVTGVN ANSSYSTTIR AINALYS
Length:237
Mass (Da):26,040
Last modified:October 31, 1997 - v1
Checksum:i004EA3E702332905
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54141 Genomic DNA. Translation: CAA90840.1.
BK006946 Genomic DNA. Translation: DAA10223.1.
RefSeqiNP_014055.1. NM_001182835.1.

Genome annotation databases

EnsemblFungiiYMR322C; YMR322C; YMR322C.
GeneIDi855372.
KEGGisce:YMR322C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54141 Genomic DNA. Translation: CAA90840.1.
BK006946 Genomic DNA. Translation: DAA10223.1.
RefSeqiNP_014055.1. NM_001182835.1.

3D structure databases

ProteinModelPortaliQ04902.
SMRiQ04902. Positions 4-237.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35501. 57 interactions.
DIPiDIP-1968N.
IntActiQ04902. 8 interactions.
MINTiMINT-386922.
STRINGi4932.YMR322C.

Protein family/group databases

MEROPSiC56.A04.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR322C; YMR322C; YMR322C.
GeneIDi855372.
KEGGisce:YMR322C.

Organism-specific databases

CYGDiYMR322c.
SGDiS000004941. SNO4.

Phylogenomic databases

GeneTreeiENSGT00390000013431.
HOGENOMiHOG000181653.
InParanoidiQ04902.
OrthoDBiEOG73547H.

Enzyme and pathway databases

BioCyciYEAST:G3O-32985-MONOMER.

Miscellaneous databases

NextBioi979157.

Gene expression databases

GenevestigatoriQ04902.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR002818. ThiJ/PfpI.
[Graphical view]
PfamiPF01965. DJ-1_PfpI. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Predicting protein functions from redundancies in large-scale protein interaction networks."
    Samanta M.P., Liang S.
    Proc. Natl. Acad. Sci. U.S.A. 100:12579-12583(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE NAME.
  4. "The 1.8-A resolution crystal structure of YDR533Cp from Saccharomyces cerevisiae: a member of the DJ-1/ThiJ/PfpI superfamily."
    Wilson M.A., St Amour C.V., Collins J.L., Ringe D., Petsko G.A.
    Proc. Natl. Acad. Sci. U.S.A. 101:1531-1536(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE NAME.
  5. "Yeast DJ-1 superfamily members are required for diauxic-shift reprogramming and cell survival in stationary phase."
    Miller-Fleming L., Antas P., Pais T.F., Smalley J.L., Giorgini F., Outeiro T.F.
    Proc. Natl. Acad. Sci. U.S.A. 111:7012-7017(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiSNO4_YEAST
AccessioniPrimary (citable) accession number: Q04902
Secondary accession number(s): D6W0E9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1997
Last sequence update: October 31, 1997
Last modified: March 3, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.