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Reviewed, UniProtKB/Swiss-Prot Q04894 (ADH6_YEAST)

Last modified June 16, 2009. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    NADP-dependent alcohol dehydrogenase 6
    EC=1.1.1.2
Alternative name(s):
    NADP-dependent alcohol dehydrogenase VI
    ScADHVI
Gene names
Name: ADH6
Ordered Locus Names: YMR318C
ORF Names: YM9924.10C
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length360 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

NADP-dependent alcohol dehydrogenase with a broad substrate specificity.

Catalytic activity

An alcohol + NADP+ = an aldehyde + NADPH.

Cofactor

Binds 2 zinc ions per subunit By similarity.

Miscellaneous

Present with 21700 molecules/cell in log phase SD medium. Ref.3

Sequence similarities

Belongs to the zinc-containing alcohol dehydrogenase family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 360360NADP-dependent alcohol dehydrogenase 6
PRO_0000160734

Regions

Nucleotide binding187 – 1926NAD By similarity
Nucleotide binding275 – 2773NAD By similarity

Sites

Metal binding461Zinc 1; catalytic By similarity
Metal binding681Zinc 1; catalytic By similarity
Metal binding1001Zinc 2 By similarity
Metal binding1031Zinc 2 By similarity
Metal binding1061Zinc 2 By similarity
Metal binding1141Zinc 2 By similarity
Metal binding1631Zinc 1; catalytic By similarity
Binding site2151NAD By similarity
Binding site3481NAD By similarity

Amino acid modifications

Modified residue1221Phosphoserine Ref.5
Modified residue1311Phosphoserine Ref.5
Modified residue2101Phosphoserine Ref.5
Modified residue3591Phosphoserine Ref.5 Ref.4

Secondary structure

1 ....................................................................... 360
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
Q04894-1 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 3F785BE0C5ED8CF1

FASTA36039,618
        10         20         30         40         50         60 
MSYPEKFEGI AIQSHEDWKN PKKTKYDPKP FYDHDIDIKI EACGVCGSDI HCAAGHWGNM 

        70         80         90        100        110        120 
KMPLVVGHEI VGKVVKLGPK SNSGLKVGQR VGVGAQVFSC LECDRCKNDN EPYCTKFVTT 

       130        140        150        160        170        180 
YSQPYEDGYV SQGGYANYVR VHEHFVVPIP ENIPSHLAAP LLCGGLTVYS PLVRNGCGPG 

       190        200        210        220        230        240 
KKVGIVGLGG IGSMGTLISK AMGAETYVIS RSSRKREDAM KMGADHYIAT LEEGDWGEKY 

       250        260        270        280        290        300 
FDTFDLIVVC ASSLTDIDFN IMPKAMKVGG RIVSISIPEQ HEMLSLKPYG LKAVSISYSA 

       310        320        330        340        350        360 
LGSIKELNQL LKLVSEKDIK IWVETLPVGE AGVHEAFERM EKGDVRYRFT LVGYDKEFSD

« Hide

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References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed: 9169872] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]"Crystallization and preliminary X-ray analysis of NADP(H)-dependent alcohol dehydrogenases from Saccharomyces cerevisiae and Rana perezi."
Valencia E., Rosell A., Larroy C., Farres J., Biosca J.A., Fita I., Pares X., Ochoa W.F.
Acta Crystallogr. D 59:334-337(2003) [PubMed: 12554944] [Abstract]
Cited for: CRYSTALLIZATION.
[3]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-359, MASS SPECTROMETRY.
[5]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-122; SER-131; SER-210 AND SER-359, MASS SPECTROMETRY.
+Additional computationally mapped references.

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Cross-references

Sequence databases

Z54141 Genomic DNA. Translation: CAA90836.1.
PIRS59311.
RefSeqNP_014051.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1PIWX-ray3.00A/B1-360[»]
1PS0X-ray3.01A1-360[»]
1Q1NX-ray3.15A1-360[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:6308N.
IntActQ04894. 3 interactions.

Proteomic databases

PeptideAtlasQ04894.
PRIDEQ04894.

Genome annotation databases

EnsemblYMR318C. Saccharomyces cerevisiae. [Contig view]
GeneID855368.
GenomeReviewsGene locus YMR318C in contig Z71257_GR.
KEGGsce:YMR318C.
NMPDRfig|4932.3.peg.5111.

Organism-specific databases

CYGDYMR318c.
SGDS000004937. ADH6.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMQ04894.
OMAQ04894. RIVSISI.

Enzyme and pathway databases

BRENDA1.1.1.2. 250.

Gene expression databases

ArrayExpressQ04894.
GermOnlineYMR318C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR013154. ADH_GroES-like.
IPR002085. ADH_SF_Zn.
IPR013149. ADH_Zn-bd.
IPR002328. ADH_Zn_CS.
[Graphical view]
PANTHERPTHR11695. ADH_Sf_Zn. 1 hit.
PfamPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
PROSITEPS00059. ADH_ZINC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio979148.

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Entry information

Entry nameADH6_YEAST
AccessionPrimary (citable) accession number: Q04894
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 16, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents