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Q04894

- ADH6_YEAST

UniProt

Q04894 - ADH6_YEAST

Protein

NADP-dependent alcohol dehydrogenase 6

Gene

ADH6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    NADP-dependent alcohol dehydrogenase with a broad substrate specificity.

    Catalytic activityi

    An alcohol + NADP+ = an aldehyde + NADPH.

    Cofactori

    Binds 2 zinc ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi46 – 461Zinc 1; catalyticBy similarity
    Metal bindingi68 – 681Zinc 1; catalyticBy similarity
    Metal bindingi100 – 1001Zinc 2By similarity
    Metal bindingi103 – 1031Zinc 2By similarity
    Metal bindingi106 – 1061Zinc 2By similarity
    Metal bindingi114 – 1141Zinc 2By similarity
    Metal bindingi163 – 1631Zinc 1; catalyticBy similarity
    Binding sitei215 – 2151NADBy similarity
    Binding sitei348 – 3481NADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi187 – 1926NADBy similarity
    Nucleotide bindingi275 – 2773NADBy similarity

    GO - Molecular functioni

    1. alcohol dehydrogenase (NADP+) activity Source: SGD
    2. hydroxymethylfurfural reductase (NADH) activity Source: SGD
    3. hydroxymethylfurfural reductase (NADPH) activity Source: SGD
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. alcohol metabolic process Source: SGD
    2. cellular aldehyde metabolic process Source: SGD
    3. furaldehyde metabolic process Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Metal-binding, NADP, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:YMR318C-MONOMER.
    SABIO-RKQ04894.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADP-dependent alcohol dehydrogenase 6 (EC:1.1.1.2)
    Alternative name(s):
    NADP-dependent alcohol dehydrogenase VI
    ScADHVI
    Gene namesi
    Name:ADH6
    Ordered Locus Names:YMR318C
    ORF Names:YM9924.10C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR318c.
    SGDiS000004937. ADH6.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 360360NADP-dependent alcohol dehydrogenase 6PRO_0000160734Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei131 – 1311Phosphoserine1 Publication
    Modified residuei359 – 3591Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ04894.
    PaxDbiQ04894.
    PeptideAtlasiQ04894.

    Expressioni

    Gene expression databases

    GenevestigatoriQ04894.

    Interactioni

    Protein-protein interaction databases

    BioGridi35498. 36 interactions.
    DIPiDIP-6308N.
    IntActiQ04894. 1 interaction.
    MINTiMINT-696048.
    STRINGi4932.YMR318C.

    Structurei

    Secondary structure

    1
    360
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni3 – 53
    Beta strandi7 – 126
    Beta strandi15 – 173
    Beta strandi22 – 265
    Beta strandi35 – 4511
    Helixi47 – 537
    Turni54 – 574
    Beta strandi62 – 643
    Beta strandi70 – 778
    Beta strandi90 – 934
    Beta strandi95 – 984
    Beta strandi101 – 1033
    Helixi104 – 1074
    Helixi111 – 1133
    Beta strandi118 – 1225
    Beta strandi134 – 1429
    Helixi143 – 1453
    Beta strandi146 – 1483
    Helixi155 – 1584
    Helixi159 – 1624
    Helixi164 – 17411
    Beta strandi182 – 1865
    Helixi190 – 20213
    Beta strandi205 – 2139
    Helixi216 – 2216
    Beta strandi225 – 2295
    Helixi230 – 2323
    Helixi236 – 2394
    Beta strandi244 – 2496
    Beta strandi254 – 2563
    Turni259 – 2613
    Helixi262 – 2654
    Beta strandi266 – 2749
    Beta strandi284 – 2863
    Helixi288 – 2903
    Beta strandi295 – 2984
    Helixi304 – 31613
    Beta strandi323 – 3297
    Helixi330 – 34213
    Beta strandi346 – 3527
    Helixi355 – 3584

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1PIWX-ray3.00A/B1-360[»]
    1PS0X-ray3.01A1-360[»]
    1Q1NX-ray3.15A1-360[»]
    ProteinModelPortaliQ04894.
    SMRiQ04894. Positions 1-360.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ04894.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1064.
    GeneTreeiENSGT00750000118226.
    HOGENOMiHOG000294667.
    KOiK00002.
    OMAiHDIDIKI.
    OrthoDBiEOG757D7B.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.180.10. 1 hit.
    InterProiIPR013149. ADH_C.
    IPR013154. ADH_GroES-like.
    IPR002085. ADH_SF_Zn-type.
    IPR002328. ADH_Zn_CS.
    IPR011032. GroES-like.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11695. PTHR11695. 1 hit.
    PfamiPF08240. ADH_N. 1 hit.
    PF00107. ADH_zinc_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF50129. SSF50129. 1 hit.
    PROSITEiPS00059. ADH_ZINC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q04894-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSYPEKFEGI AIQSHEDWKN PKKTKYDPKP FYDHDIDIKI EACGVCGSDI    50
    HCAAGHWGNM KMPLVVGHEI VGKVVKLGPK SNSGLKVGQR VGVGAQVFSC 100
    LECDRCKNDN EPYCTKFVTT YSQPYEDGYV SQGGYANYVR VHEHFVVPIP 150
    ENIPSHLAAP LLCGGLTVYS PLVRNGCGPG KKVGIVGLGG IGSMGTLISK 200
    AMGAETYVIS RSSRKREDAM KMGADHYIAT LEEGDWGEKY FDTFDLIVVC 250
    ASSLTDIDFN IMPKAMKVGG RIVSISIPEQ HEMLSLKPYG LKAVSISYSA 300
    LGSIKELNQL LKLVSEKDIK IWVETLPVGE AGVHEAFERM EKGDVRYRFT 350
    LVGYDKEFSD 360
    Length:360
    Mass (Da):39,618
    Last modified:November 1, 1997 - v1
    Checksum:i3F785BE0C5ED8CF1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z54141 Genomic DNA. Translation: CAA90836.1.
    BK006946 Genomic DNA. Translation: DAA10220.1.
    PIRiS59311.
    RefSeqiNP_014051.3. NM_001182831.3.

    Genome annotation databases

    EnsemblFungiiYMR318C; YMR318C; YMR318C.
    GeneIDi855368.
    KEGGisce:YMR318C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z54141 Genomic DNA. Translation: CAA90836.1 .
    BK006946 Genomic DNA. Translation: DAA10220.1 .
    PIRi S59311.
    RefSeqi NP_014051.3. NM_001182831.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1PIW X-ray 3.00 A/B 1-360 [» ]
    1PS0 X-ray 3.01 A 1-360 [» ]
    1Q1N X-ray 3.15 A 1-360 [» ]
    ProteinModelPortali Q04894.
    SMRi Q04894. Positions 1-360.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35498. 36 interactions.
    DIPi DIP-6308N.
    IntActi Q04894. 1 interaction.
    MINTi MINT-696048.
    STRINGi 4932.YMR318C.

    Proteomic databases

    MaxQBi Q04894.
    PaxDbi Q04894.
    PeptideAtlasi Q04894.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR318C ; YMR318C ; YMR318C .
    GeneIDi 855368.
    KEGGi sce:YMR318C.

    Organism-specific databases

    CYGDi YMR318c.
    SGDi S000004937. ADH6.

    Phylogenomic databases

    eggNOGi COG1064.
    GeneTreei ENSGT00750000118226.
    HOGENOMi HOG000294667.
    KOi K00002.
    OMAi HDIDIKI.
    OrthoDBi EOG757D7B.

    Enzyme and pathway databases

    BioCyci YEAST:YMR318C-MONOMER.
    SABIO-RK Q04894.

    Miscellaneous databases

    EvolutionaryTracei Q04894.
    NextBioi 979148.

    Gene expression databases

    Genevestigatori Q04894.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.180.10. 1 hit.
    InterProi IPR013149. ADH_C.
    IPR013154. ADH_GroES-like.
    IPR002085. ADH_SF_Zn-type.
    IPR002328. ADH_Zn_CS.
    IPR011032. GroES-like.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11695. PTHR11695. 1 hit.
    Pfami PF08240. ADH_N. 1 hit.
    PF00107. ADH_zinc_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50129. SSF50129. 1 hit.
    PROSITEi PS00059. ADH_ZINC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Crystallization and preliminary X-ray analysis of NADP(H)-dependent alcohol dehydrogenases from Saccharomyces cerevisiae and Rana perezi."
      Valencia E., Rosell A., Larroy C., Farres J., Biosca J.A., Fita I., Pares X., Ochoa W.F.
      Acta Crystallogr. D 59:334-337(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: CRYSTALLIZATION.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-131 AND SER-359, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-359, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiADH6_YEAST
    AccessioniPrimary (citable) accession number: Q04894
    Secondary accession number(s): D6W0E6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 21700 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3