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Q04868

- ELP6_YEAST

UniProt

Q04868 - ELP6_YEAST

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Protein

Elongator complex protein 6

Gene

ELP6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as component of the RNA polymerase II elongator complex, which is a major histone acetyltransferase component of the RNA polymerase II (RNAPII) holoenzyme and is involved in transcriptional elongation. Association with elongating RNAPII requires a hyperphosphorylated state of the RNAPII C-terminal domain (CTD). Elongator binds to both naked and nucleosomal DNA, can acetylate both core and nucleosomal histones, and is involved in chromatin remodeling. It acetylates histones H3, preferentially at 'Lys-14', and H4, preferentially at 'Lys-8'. It functions as a gamma-toxin target (TOT); disruption of the complex confers resistance to Kluyveromyces lactis toxin zymocin (pGKL1 killer toxin). May also be involved in sensitiviy to Pichia inositovora toxin. May be involved in tRNA modification. ELP6 is required for an early step in synthesis of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) groups present on uridines at the wobble position in tRNA.6 Publications

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. protein urmylation Source: SGD
  3. regulation of transcription from RNA polymerase II promoter Source: SGD
  4. transcription, DNA-templated Source: UniProtKB-KW
  5. tRNA wobble uridine modification Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-32976-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongator complex protein 6
Alternative name(s):
Gamma-toxin target 6
HAT-associated protein 3
Gene namesi
Name:ELP6
Synonyms:HAP3, TOT6
Ordered Locus Names:YMR312W
ORF Names:YM9924.04
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR312w.
SGDiS000004929. ELP6.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. Elongator holoenzyme complex Source: SGD
  3. nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 273273Elongator complex protein 6PRO_0000203355Add
BLAST

Proteomic databases

MaxQBiQ04868.
PaxDbiQ04868.
PeptideAtlasiQ04868.

Expressioni

Gene expression databases

GenevestigatoriQ04868.

Interactioni

Subunit structurei

Component of the RNA polymerase II elongator complex, which consists of IKI3, ELP2, ELP3, ELP4, IKI1 and ELP6. ELP4, IKI1 and ELP6 form a distinct subcomplex (HAP).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ELP4Q0288410EBI-27653,EBI-35277
IKI1P388746EBI-27653,EBI-9061

Protein-protein interaction databases

BioGridi35492. 214 interactions.
DIPiDIP-1966N.
IntActiQ04868. 8 interactions.
MINTiMINT-385455.
STRINGi4932.YMR312W.

Structurei

Secondary structure

1
273
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi20 – 234Combined sources
Beta strandi25 – 273Combined sources
Beta strandi30 – 367Combined sources
Helixi43 – 5513Combined sources
Helixi59 – 624Combined sources
Beta strandi76 – 849Combined sources
Helixi86 – 9510Combined sources
Helixi100 – 1023Combined sources
Beta strandi103 – 1097Combined sources
Helixi112 – 1165Combined sources
Turni117 – 1193Combined sources
Helixi122 – 13817Combined sources
Beta strandi143 – 1497Combined sources
Helixi151 – 1533Combined sources
Helixi154 – 1574Combined sources
Helixi163 – 1697Combined sources
Helixi171 – 1766Combined sources
Beta strandi179 – 1868Combined sources
Helixi188 – 1903Combined sources
Helixi197 – 2004Combined sources
Helixi202 – 21413Combined sources
Beta strandi216 – 2227Combined sources
Beta strandi234 – 2407Combined sources
Beta strandi257 – 2648Combined sources
Beta strandi267 – 2715Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4A8JX-ray2.10C/F1-273[»]
4EJSX-ray2.61C1-273[»]
ProteinModelPortaliQ04868.
SMRiQ04868. Positions 2-272.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ELP6 family.Curated

Phylogenomic databases

eggNOGiNOG302847.
HOGENOMiHOG000248084.
InParanoidiQ04868.
KOiK11377.
OMAiDFLVKLH.
OrthoDBiEOG7QC87F.

Sequencei

Sequence statusi: Complete.

Q04868-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGSVQRQDLV LFSDQSVLPA HFFQDSNSHN LFFITHQSCT QPLWMINALV
60 70 80 90 100
ETHVLGSPSS LNESSSSMLP SSTRSHAVLA SFIHEQNYFT NSLNKLKIPS
110 120 130 140 150
NNYNVLDFLS DFIVNNIHNK PRDKILSDVL AKFSAAIQNN PTDTIVIIEQ
160 170 180 190 200
PELLLSLVSG LTCSELNNKF ITPLLRQCKV LIIVSNSDIF NIDEYDASVH
210 220 230 240 250
SSNLQNFYKS SFIKSMINLN LNPLKTGFAK DVTGSLHVCR GGAPIATSNT
260 270
SLHVVENEYL YLNEKESTKL FYR
Length:273
Mass (Da):30,574
Last modified:November 1, 1997 - v1
Checksum:i1A5062465AF67F47
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54141 Genomic DNA. Translation: CAA90830.1.
AY557983 Genomic DNA. Translation: AAS56309.1.
BK006946 Genomic DNA. Translation: DAA10213.1.
PIRiS59305.
RefSeqiNP_014043.1. NM_001182823.1.

Genome annotation databases

EnsemblFungiiYMR312W; YMR312W; YMR312W.
GeneIDi855360.
KEGGisce:YMR312W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54141 Genomic DNA. Translation: CAA90830.1 .
AY557983 Genomic DNA. Translation: AAS56309.1 .
BK006946 Genomic DNA. Translation: DAA10213.1 .
PIRi S59305.
RefSeqi NP_014043.1. NM_001182823.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4A8J X-ray 2.10 C/F 1-273 [» ]
4EJS X-ray 2.61 C 1-273 [» ]
ProteinModelPortali Q04868.
SMRi Q04868. Positions 2-272.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35492. 214 interactions.
DIPi DIP-1966N.
IntActi Q04868. 8 interactions.
MINTi MINT-385455.
STRINGi 4932.YMR312W.

Proteomic databases

MaxQBi Q04868.
PaxDbi Q04868.
PeptideAtlasi Q04868.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR312W ; YMR312W ; YMR312W .
GeneIDi 855360.
KEGGi sce:YMR312W.

Organism-specific databases

CYGDi YMR312w.
SGDi S000004929. ELP6.

Phylogenomic databases

eggNOGi NOG302847.
HOGENOMi HOG000248084.
InParanoidi Q04868.
KOi K11377.
OMAi DFLVKLH.
OrthoDBi EOG7QC87F.

Enzyme and pathway databases

BioCyci YEAST:G3O-32976-MONOMER.

Miscellaneous databases

NextBioi 979130.

Gene expression databases

Genevestigatori Q04868.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Elongator, a multisubunit component of a novel RNA polymerase II holoenzyme for transcriptional elongation."
    Otero G., Fellows J., Li Y., de Bizemont T., Dirac A.M., Gustafsson C.M., Erdjument-Bromage H., Tempst P., Svejstrup J.Q.
    Mol. Cell 3:109-118(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN TRANSCRIPTIONAL REGULATION, SUBCELLULAR LOCATION.
  5. "Saccharomyces cerevisiae Elongator mutations confer resistance to the Kluyveromyces lactis zymocin."
    Frohloff F., Fichtner L., Jablonowski D., Breunig K.D., Schaffrath R.
    EMBO J. 20:1993-2003(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN ZYMOCIN SENSITIVITY.
  6. "RNA polymerase II elongator holoenzyme is composed of two discrete subcomplexes."
    Winkler G.S., Petrakis T.G., Ethelberg S., Tokunaga M., Erdjument-Bromage H., Tempst P., Svejstrup J.Q.
    J. Biol. Chem. 276:32743-32749(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE RNA POLYMERASE II ELONGATOR COMPLEX.
  7. "A multiprotein complex that interacts with RNA polymerase II elongator."
    Li Y., Takagi Y., Jiang Y., Tokunaga M., Erdjument-Bromage H., Tempst P., Kornberg R.D.
    J. Biol. Chem. 276:29628-29631(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE RNA POLYMERASE II ELONGATOR COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  8. "Characterization of a six-subunit holo-elongator complex required for the regulated expression of a group of genes in Saccharomyces cerevisiae."
    Krogan N.J., Greenblatt J.F.
    Mol. Cell. Biol. 21:8203-8212(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE RNA POLYMERASE II ELONGATOR COMPLEX, FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN TRANSCRIPTIONAL REGULATION.
  9. "Elongator is a histone H3 and H4 acetyltransferase important for normal histone acetylation levels in vivo."
    Winkler G.S., Kristjuhan A., Erdjument-Bromage H., Tempst P., Svejstrup J.Q.
    Proc. Natl. Acad. Sci. U.S.A. 99:3517-3522(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN HISTONE ACETYLATION.
  10. "Structural and functional analysis of the killer element pPin1-3 from Pichia inositovora."
    Klassen R., Meinhardt F.
    Mol. Genet. Genomics 270:190-199(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN PICHIA INOSITOVORA TOXIN SENSITIVITY.
  11. "Elp1p, the yeast homolog of the FD disease syndrome protein, negatively regulates exocytosis independently of transcriptional elongation."
    Rahl P.B., Chen C.Z., Collins R.N.
    Mol. Cell 17:841-853(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  12. "An early step in wobble uridine tRNA modification requires the Elongator complex."
    Huang B., Johansson M.J.O., Bystroem A.S.
    RNA 11:424-436(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN TRNA MODIFICATION.

Entry informationi

Entry nameiELP6_YEAST
AccessioniPrimary (citable) accession number: Q04868
Secondary accession number(s): D6W0D9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 26, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3