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Q04868

- ELP6_YEAST

UniProt

Q04868 - ELP6_YEAST

Protein

Elongator complex protein 6

Gene

ELP6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Acts as component of the RNA polymerase II elongator complex, which is a major histone acetyltransferase component of the RNA polymerase II (RNAPII) holoenzyme and is involved in transcriptional elongation. Association with elongating RNAPII requires a hyperphosphorylated state of the RNAPII C-terminal domain (CTD). Elongator binds to both naked and nucleosomal DNA, can acetylate both core and nucleosomal histones, and is involved in chromatin remodeling. It acetylates histones H3, preferentially at 'Lys-14', and H4, preferentially at 'Lys-8'. It functions as a gamma-toxin target (TOT); disruption of the complex confers resistance to Kluyveromyces lactis toxin zymocin (pGKL1 killer toxin). May also be involved in sensitiviy to Pichia inositovora toxin. May be involved in tRNA modification. ELP6 is required for an early step in synthesis of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) groups present on uridines at the wobble position in tRNA.6 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. protein urmylation Source: SGD
    3. regulation of transcription from RNA polymerase II promoter Source: SGD
    4. transcription, DNA-templated Source: UniProtKB-KW
    5. tRNA wobble uridine modification Source: SGD

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32976-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Elongator complex protein 6
    Alternative name(s):
    Gamma-toxin target 6
    HAT-associated protein 3
    Gene namesi
    Name:ELP6
    Synonyms:HAP3, TOT6
    Ordered Locus Names:YMR312W
    ORF Names:YM9924.04
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR312w.
    SGDiS000004929. ELP6.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. Elongator holoenzyme complex Source: SGD
    3. nucleoplasm Source: Reactome

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 273273Elongator complex protein 6PRO_0000203355Add
    BLAST

    Proteomic databases

    MaxQBiQ04868.
    PaxDbiQ04868.
    PeptideAtlasiQ04868.

    Expressioni

    Gene expression databases

    GenevestigatoriQ04868.

    Interactioni

    Subunit structurei

    Component of the RNA polymerase II elongator complex, which consists of IKI3, ELP2, ELP3, ELP4, IKI1 and ELP6. ELP4, IKI1 and ELP6 form a distinct subcomplex (HAP).3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ELP4Q0288410EBI-27653,EBI-35277
    IKI1P388746EBI-27653,EBI-9061

    Protein-protein interaction databases

    BioGridi35492. 213 interactions.
    DIPiDIP-1966N.
    IntActiQ04868. 8 interactions.
    MINTiMINT-385455.
    STRINGi4932.YMR312W.

    Structurei

    Secondary structure

    1
    273
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi20 – 234
    Beta strandi25 – 273
    Beta strandi30 – 367
    Helixi43 – 5513
    Helixi59 – 624
    Beta strandi76 – 849
    Helixi86 – 9510
    Helixi100 – 1023
    Beta strandi103 – 1097
    Helixi112 – 1165
    Turni117 – 1193
    Helixi122 – 13817
    Beta strandi143 – 1497
    Helixi151 – 1533
    Helixi154 – 1574
    Helixi163 – 1697
    Helixi171 – 1766
    Beta strandi179 – 1868
    Helixi188 – 1903
    Helixi197 – 2004
    Helixi202 – 21413
    Beta strandi216 – 2227
    Beta strandi234 – 2407
    Beta strandi257 – 2648
    Beta strandi267 – 2715

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4A8JX-ray2.10C/F1-273[»]
    4EJSX-ray2.61C1-273[»]
    ProteinModelPortaliQ04868.
    SMRiQ04868. Positions 2-272.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ELP6 family.Curated

    Phylogenomic databases

    eggNOGiNOG302847.
    HOGENOMiHOG000248084.
    KOiK11377.
    OMAiDFLVKLH.
    OrthoDBiEOG7QC87F.

    Sequencei

    Sequence statusi: Complete.

    Q04868-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSVQRQDLV LFSDQSVLPA HFFQDSNSHN LFFITHQSCT QPLWMINALV    50
    ETHVLGSPSS LNESSSSMLP SSTRSHAVLA SFIHEQNYFT NSLNKLKIPS 100
    NNYNVLDFLS DFIVNNIHNK PRDKILSDVL AKFSAAIQNN PTDTIVIIEQ 150
    PELLLSLVSG LTCSELNNKF ITPLLRQCKV LIIVSNSDIF NIDEYDASVH 200
    SSNLQNFYKS SFIKSMINLN LNPLKTGFAK DVTGSLHVCR GGAPIATSNT 250
    SLHVVENEYL YLNEKESTKL FYR 273
    Length:273
    Mass (Da):30,574
    Last modified:November 1, 1997 - v1
    Checksum:i1A5062465AF67F47
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z54141 Genomic DNA. Translation: CAA90830.1.
    AY557983 Genomic DNA. Translation: AAS56309.1.
    BK006946 Genomic DNA. Translation: DAA10213.1.
    PIRiS59305.
    RefSeqiNP_014043.1. NM_001182823.1.

    Genome annotation databases

    EnsemblFungiiYMR312W; YMR312W; YMR312W.
    GeneIDi855360.
    KEGGisce:YMR312W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z54141 Genomic DNA. Translation: CAA90830.1 .
    AY557983 Genomic DNA. Translation: AAS56309.1 .
    BK006946 Genomic DNA. Translation: DAA10213.1 .
    PIRi S59305.
    RefSeqi NP_014043.1. NM_001182823.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4A8J X-ray 2.10 C/F 1-273 [» ]
    4EJS X-ray 2.61 C 1-273 [» ]
    ProteinModelPortali Q04868.
    SMRi Q04868. Positions 2-272.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35492. 213 interactions.
    DIPi DIP-1966N.
    IntActi Q04868. 8 interactions.
    MINTi MINT-385455.
    STRINGi 4932.YMR312W.

    Proteomic databases

    MaxQBi Q04868.
    PaxDbi Q04868.
    PeptideAtlasi Q04868.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR312W ; YMR312W ; YMR312W .
    GeneIDi 855360.
    KEGGi sce:YMR312W.

    Organism-specific databases

    CYGDi YMR312w.
    SGDi S000004929. ELP6.

    Phylogenomic databases

    eggNOGi NOG302847.
    HOGENOMi HOG000248084.
    KOi K11377.
    OMAi DFLVKLH.
    OrthoDBi EOG7QC87F.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32976-MONOMER.

    Miscellaneous databases

    NextBioi 979130.

    Gene expression databases

    Genevestigatori Q04868.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Elongator, a multisubunit component of a novel RNA polymerase II holoenzyme for transcriptional elongation."
      Otero G., Fellows J., Li Y., de Bizemont T., Dirac A.M., Gustafsson C.M., Erdjument-Bromage H., Tempst P., Svejstrup J.Q.
      Mol. Cell 3:109-118(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN TRANSCRIPTIONAL REGULATION, SUBCELLULAR LOCATION.
    5. "Saccharomyces cerevisiae Elongator mutations confer resistance to the Kluyveromyces lactis zymocin."
      Frohloff F., Fichtner L., Jablonowski D., Breunig K.D., Schaffrath R.
      EMBO J. 20:1993-2003(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN ZYMOCIN SENSITIVITY.
    6. "RNA polymerase II elongator holoenzyme is composed of two discrete subcomplexes."
      Winkler G.S., Petrakis T.G., Ethelberg S., Tokunaga M., Erdjument-Bromage H., Tempst P., Svejstrup J.Q.
      J. Biol. Chem. 276:32743-32749(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE RNA POLYMERASE II ELONGATOR COMPLEX.
    7. "A multiprotein complex that interacts with RNA polymerase II elongator."
      Li Y., Takagi Y., Jiang Y., Tokunaga M., Erdjument-Bromage H., Tempst P., Kornberg R.D.
      J. Biol. Chem. 276:29628-29631(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE RNA POLYMERASE II ELONGATOR COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    8. "Characterization of a six-subunit holo-elongator complex required for the regulated expression of a group of genes in Saccharomyces cerevisiae."
      Krogan N.J., Greenblatt J.F.
      Mol. Cell. Biol. 21:8203-8212(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE RNA POLYMERASE II ELONGATOR COMPLEX, FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN TRANSCRIPTIONAL REGULATION.
    9. "Elongator is a histone H3 and H4 acetyltransferase important for normal histone acetylation levels in vivo."
      Winkler G.S., Kristjuhan A., Erdjument-Bromage H., Tempst P., Svejstrup J.Q.
      Proc. Natl. Acad. Sci. U.S.A. 99:3517-3522(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN HISTONE ACETYLATION.
    10. "Structural and functional analysis of the killer element pPin1-3 from Pichia inositovora."
      Klassen R., Meinhardt F.
      Mol. Genet. Genomics 270:190-199(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN PICHIA INOSITOVORA TOXIN SENSITIVITY.
    11. "Elp1p, the yeast homolog of the FD disease syndrome protein, negatively regulates exocytosis independently of transcriptional elongation."
      Rahl P.B., Chen C.Z., Collins R.N.
      Mol. Cell 17:841-853(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    12. "An early step in wobble uridine tRNA modification requires the Elongator complex."
      Huang B., Johansson M.J.O., Bystroem A.S.
      RNA 11:424-436(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN TRNA MODIFICATION.

    Entry informationi

    Entry nameiELP6_YEAST
    AccessioniPrimary (citable) accession number: Q04868
    Secondary accession number(s): D6W0D9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Uncharacterized protein families (UPF)
      List of uncharacterized protein family (UPF) entries
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3