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Protein

Chain length determinant protein

Gene

wzzB

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Confers a modal distribution of chain length on the O-antigen component of lipopolysaccharide (LPS). Gives rise to a reduced number of short chain molecules and increases in numbers of longer molecules, with a modal value of 20.

Pathwayi: lipopolysaccharide biosynthesis

This protein is involved in the pathway lipopolysaccharide biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway lipopolysaccharide biosynthesis and in Bacterial outer membrane biogenesis.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciSENT99287:GCTI-2091-MONOMER.
UniPathwayiUPA00030.

Names & Taxonomyi

Protein namesi
Recommended name:
Chain length determinant protein
Alternative name(s):
Polysaccharide antigen chain regulator
Gene namesi
Name:wzzB
Synonyms:cld, rol
Ordered Locus Names:STM2079
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3131CytoplasmicSequence analysisAdd
BLAST
Transmembranei32 – 5221HelicalSequence analysisAdd
BLAST
Topological domaini53 – 294242PeriplasmicSequence analysisAdd
BLAST
Transmembranei295 – 31521HelicalSequence analysisAdd
BLAST
Topological domaini316 – 32712CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 327327Chain length determinant proteinPRO_0000065993Add
BLAST

Proteomic databases

PaxDbiQ04866.
PRIDEiQ04866.

Interactioni

Protein-protein interaction databases

DIPiDIP-46396N.
STRINGi99287.STM2079.

Structurei

Secondary structure

1
327
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi56 – 638Combined sources
Helixi68 – 703Combined sources
Helixi71 – 8111Combined sources
Helixi88 – 11124Combined sources
Beta strandi112 – 1154Combined sources
Beta strandi118 – 1258Combined sources
Beta strandi132 – 1409Combined sources
Helixi141 – 19959Combined sources
Helixi201 – 21010Combined sources
Turni225 – 2273Combined sources
Helixi228 – 2314Combined sources
Helixi233 – 2419Combined sources
Helixi243 – 2453Combined sources
Helixi252 – 26615Combined sources
Turni271 – 2733Combined sources
Beta strandi278 – 2814Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3B8PX-ray3.10A/B/C/D/E54-294[»]
4E29X-ray1.60A/B201-293[»]
4E2CX-ray2.80A/B256-291[»]
ProteinModelPortaliQ04866.
SMRiQ04866. Positions 54-292.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ04866.

Family & Domainsi

Sequence similaritiesi

Belongs to the WzzB/Cld/Rol family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108KPN. Bacteria.
COG3765. LUCA.
HOGENOMiHOG000121918.
KOiK05789.
OMAiDNIALRM.
OrthoDBiEOG683S6P.
PhylomeDBiQ04866.

Family and domain databases

InterProiIPR003856. LipoPS_biosynth.
[Graphical view]
PfamiPF02706. Wzz. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04866-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVDSNTSSG RGNDPEQIDL IELLLQLWRG KMTIIVAVII AILLAVGYLM
60 70 80 90 100
IAKEKWTSTA IITQPDAAQV ATYTNALNVL YGGNAPKISE VQANFISRFS
110 120 130 140 150
SAFSALSEVL DNQKEREKLT IEQSVKGQAL PLSVSYVSTT AEGAQRRLAE
160 170 180 190 200
YIQQVDEEVA KELEVDLKDN ITLQTKTLQE SLETQEVVAQ EQKDLRIKQI
210 220 230 240 250
EEALRYADEA KITQPQIQQT QDVTQDTMFL LGSDALKSMI QNEATRPLVF
260 270 280 290 300
SPAYYQTKQT LLDIKNLKVT ADTVHVYRYV MKPTLPVRRD SPKTAITLVL
310 320
AVLLGGMIGA GIVLGRNALR SYKPKAL
Length:327
Mass (Da):36,259
Last modified:February 1, 1994 - v1
Checksum:i72D8BA8A3F4DF1A9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti50 – 501M → I (PubMed:1379582).Curated
Sequence conflicti136 – 1361Y → D (PubMed:1379582).Curated
Sequence conflicti158 – 1592EV → QL (PubMed:1379582).Curated
Sequence conflicti164 – 1641E → Q (PubMed:1379582).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z17278 Genomic DNA. Translation: CAA78946.1.
M89933 Genomic DNA. No translation available.
AE006468 Genomic DNA. Translation: AAL20983.1.
RefSeqiNP_461024.1. NC_003197.1.
WP_000215261.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL20983; AAL20983; STM2079.
GeneIDi1253600.
KEGGistm:STM2079.
PATRICi32382741. VBISalEnt20916_2201.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z17278 Genomic DNA. Translation: CAA78946.1.
M89933 Genomic DNA. No translation available.
AE006468 Genomic DNA. Translation: AAL20983.1.
RefSeqiNP_461024.1. NC_003197.1.
WP_000215261.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3B8PX-ray3.10A/B/C/D/E54-294[»]
4E29X-ray1.60A/B201-293[»]
4E2CX-ray2.80A/B256-291[»]
ProteinModelPortaliQ04866.
SMRiQ04866. Positions 54-292.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46396N.
STRINGi99287.STM2079.

Proteomic databases

PaxDbiQ04866.
PRIDEiQ04866.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL20983; AAL20983; STM2079.
GeneIDi1253600.
KEGGistm:STM2079.
PATRICi32382741. VBISalEnt20916_2201.

Phylogenomic databases

eggNOGiENOG4108KPN. Bacteria.
COG3765. LUCA.
HOGENOMiHOG000121918.
KOiK05789.
OMAiDNIALRM.
OrthoDBiEOG683S6P.
PhylomeDBiQ04866.

Enzyme and pathway databases

UniPathwayiUPA00030.
BioCyciSENT99287:GCTI-2091-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ04866.

Family and domain databases

InterProiIPR003856. LipoPS_biosynth.
[Graphical view]
PfamiPF02706. Wzz. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Repeat unit polysaccharides of bacteria: a model for polymerization resembling that of ribosomes and fatty acid synthetase, with a novel mechanism for determining chain length."
    Bastin D.A., Stevenson G., Brown P.K., Haase A., Reeves P.R.
    Mol. Microbiol. 7:725-734(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LT2.
  2. "Nucleotide sequences of the genes regulating O-polysaccharide antigen chain length (rol) from Escherichia coli and Salmonella typhimurium: protein homology and functional complementation."
    Batchelor R.A., Alifano P., Biffali E., Hull S.I., Hull R.A.
    J. Bacteriol. 174:5228-5236(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LT2.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.

Entry informationi

Entry nameiWZZB_SALTY
AccessioniPrimary (citable) accession number: Q04866
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: December 9, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.