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Protein

Nuclear factor NF-kappa-B p105 subunit

Gene

NFKB1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

P105 is the precursor of the p50 subunit of the nuclear factor NF-kappa-B, which binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. The precursor protein itself does not bind to DNA.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-GGA-1169091 Activation of NF-kappaB in B cells
R-GGA-1227892 TRAF6 mediated NF-kB activation
R-GGA-1810476 RIP-mediated NFkB activation via ZBP1
R-GGA-193692 Regulated proteolysis of p75NTR
R-GGA-202424 Downstream TCR signaling
R-GGA-209560 NF-kB is activated and signals survival
R-GGA-2871837 FCERI mediated NF-kB activation
R-GGA-3134963 DEx/H-box helicases activate type I IFN and inflammatory cytokines production
R-GGA-3214841 PKMTs methylate histone lysines
R-GGA-434001 TAK1 activates NFkB by phosphorylation and activation of IKKs complex
R-GGA-434131 NFkB activation mediated by RIP1 complexed with activated TLR3
R-GGA-445989 TAK1 activates NFkB by phosphorylation and activation of IKKs complex
R-GGA-448706 Interleukin-1 processing
R-GGA-5607764 CLEC7A (Dectin-1) signaling
R-GGA-5621575 CD209 (DC-SIGN) signaling
R-GGA-5684264 MAP3K8 (TPL2)-dependent MAPK1/3 activation
R-GGA-6798695 Neutrophil degranulation
R-GGA-9020702 Interleukin-1 signaling
R-GGA-933542 TRAF6 mediated NF-kB activation

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear factor NF-kappa-B p105 subunit
Alternative name(s):
Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
Cleaved into the following chain:
Gene namesi
Name:NFKB1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000303161 – 983Nuclear factor NF-kappa-B p105 subunitAdd BLAST983
ChainiPRO_00000303171 – ?Nuclear factor NF-kappa-B p50 subunitBy similarity

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei66S-nitrosocysteineBy similarity1
Modified residuei342Phosphoserine; by PKASequence analysis1
Modified residuei938PhosphoserineBy similarity1

Post-translational modificationi

While translation occurs, the particular unfolded structure after the GRR repeat promotes the generation of p50 making it an acceptable substrate for the proteasome. This process is known as cotranslational processing. The processed form is active and the unprocessed form acts as an inhibitor (I kappa B-like), being able to form cytosolic complexes with NF-kappa B, trapping it in the cytoplasm. Complete folding of the region downstream of the GRR repeat precludes processing (By similarity).By similarity
S-nitrosylation of Cys-66 affects DNA binding.By similarity

Keywords - PTMi

Phosphoprotein, S-nitrosylation

Proteomic databases

PaxDbiQ04861
PRIDEiQ04861

Expressioni

Gene expression databases

BgeeiENSGALG00000012304

Interactioni

Subunit structurei

Active NF-kappa-B is a heterodimer of an about 50 kDa DNA-binding subunit and the weak DNA-binding subunit p65. Two heterodimers might form a labile tetramer.

GO - Molecular functioni

  • NF-kappaB binding Source: UniProtKB

Protein-protein interaction databases

STRINGi9031.ENSGALP00000020084

Structurei

3D structure databases

SMRiQ04861
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini47 – 372RHDPROSITE-ProRule annotationAdd BLAST326
Repeati540 – 569ANK 1Add BLAST30
Repeati579 – 608ANK 2Add BLAST30
Repeati612 – 641ANK 3Add BLAST30
Repeati648 – 677ANK 4Add BLAST30
Repeati682 – 712ANK 5Add BLAST31
Repeati716 – 745ANK 6Add BLAST30
Repeati769 – 799ANK 7Add BLAST31
Domaini804 – 891DeathAdd BLAST88

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni377 – 397GRRAdd BLAST21

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi365 – 370Nuclear localization signalSequence analysis6

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi378 – 414Gly-richAdd BLAST37

Domaini

The C-terminus of p105 might be involved in cytoplasmic retention, inhibition of DNA-binding by p50 homodimers, and/or transcription activation.

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0504 Eukaryota
COG0666 LUCA
GeneTreeiENSGT00500000044765
HOGENOMiHOG000004822
HOVERGENiHBG052613
InParanoidiQ04861
KOiK02580
OMAiHEQMFHL
OrthoDBiEOG091G03PF

Family and domain databases

CDDicd00204 ANK, 2 hits
cd01177 IPT_NFkappaB, 1 hit
Gene3Di1.25.40.20, 1 hit
2.60.40.10, 1 hit
2.60.40.340, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR011029 DEATH-like_dom_sf
IPR000488 Death_domain
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
IPR002909 IPT_dom
IPR033926 IPT_NFkappaB
IPR030503 NF-kB_p105
IPR000451 NFkB/Dor
IPR008967 p53-like_TF_DNA-bd
IPR030492 RHD_CS
IPR032397 RHD_dimer
IPR011539 RHD_DNA_bind_dom
IPR037059 RHD_DNA_bind_dom_sf
PANTHERiPTHR24169 PTHR24169, 1 hit
PTHR24169:SF9 PTHR24169:SF9, 1 hit
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PF00531 Death, 1 hit
PF16179 RHD_dimer, 1 hit
PF00554 RHD_DNA_bind, 1 hit
PRINTSiPR00057 NFKBTNSCPFCT
SMARTiView protein in SMART
SM00248 ANK, 7 hits
SM00005 DEATH, 1 hit
SM00429 IPT, 1 hit
SUPFAMiSSF47986 SSF47986, 1 hit
SSF48403 SSF48403, 1 hit
SSF49417 SSF49417, 1 hit
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 5 hits
PS01204 REL_1, 1 hit
PS50254 REL_2, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q04861-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGEDPYIMG VSDPQMFAMD QLMGMSTIFN NTGYITSDLP LRTADGPYLQ
60 70 80 90 100
IIEQPKQRGF RFRYVCEGPS HGGLPGASSE KNKKSYPQVK ICNYVGPAKV
110 120 130 140 150
IVQLVTNGKY VHLHAHSLVG KFCEDGVCTV NAGPKDMVVG FANLGILHVT
160 170 180 190 200
KKKVFETLET RMIDACKKGY NPGLLVHPEL GYLQAEGCGD RQLTEREREI
210 220 230 240 250
IRQAAVQQTK EMDLSVVRLM FTAFLPDSNG GFTRRLDPVI SDAIYDSKAP
260 270 280 290 300
NASNLKIVRM DRTAGCVTGG EEIYLLCDKV QKDDIQIRFY EEDENGGMWE
310 320 330 340 350
GFGDFSPTDV HRQFAIVFKT PKYRDVNITK PASVFVQLRR KSDLETSEPK
360 370 380 390 400
PFLYYPEIKD KEEVQRKRQK LMPNFSDGYG GGSGAGGGGM FGGGGGGAGS
410 420 430 440 450
GFSYPSYGYS AFGGMHFHPG TTKSNAGMKH ELSNSTVKKD EESSDKQSDK
460 470 480 490 500
WDTKHDVKVE TVEKNECRTS GHNEEKEDAS LCCKDEGNKP KCGCQDGLFL
510 520 530 540 550
EKAMQLAKRH CNALFDYAVT GDVRMLLAVQ RHLTAVQDDN GDNVLHLSII
560 570 580 590 600
HLHRELVKNL LEVMPDMNYN NIINMRNDLY QTPLHLAVIT KQAEVVEDLL
610 620 630 640 650
KAGANVNLLD RHGNSVLHLA AAEGDDKILS LLLKHQKASS MIDLSNGEGL
660 670 680 690 700
SAIHMVVTAN SLSCLKLLIA AGVDVNAQEQ KSGRTALHLA VEQENVPLAG
710 720 730 740 750
CLLLEGDADV DSTTYDGTTP LHIAAGRGFT KLAAVLKAAG ADPHVENFEP
760 770 780 790 800
LFDVEEDVKD DDDDEGIVPG TTPLDMAANW EVYDILNGKP YIAAAAVSED
810 820 830 840 850
LLSQGPLREL NESSKQQLYK LLETPDPSKN WSTLAEKLGL GILNNAFRLS
860 870 880 890 900
PSPSKTLLDN YKISGGTVQE LIAALTQMDH TEAIEVIQKA LSSSQRQSHQ
910 920 930 940 950
EDNTIEAFPS LSPTSFAKEE TGELYNHKFQ DPESTCDSGV ETSFRKLSFT
960 970 980
YSDSLNSKSS ITLSKMTLGY RKAQCKAVIY LTR
Length:983
Mass (Da):107,992
Last modified:April 18, 2012 - v2
Checksum:iB8885C7716393285
GO
Isoform 2 (identifier: Q04861-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     970-970: Y → YG

Show »
Length:984
Mass (Da):108,049
Checksum:i2393F098A129B72C
GO
Isoform 3 (identifier: Q04861-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     971-983: RKAQCKAVIYLTR → GQESSVQSSYIPN

Show »
Length:983
Mass (Da):107,838
Checksum:i259C140FE1DF5994
GO

Sequence cautioni

The sequence AAB58343 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAB58343 differs from that shown. Reason: Erroneous translation. Wrong genetic code used for translating the sequence.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti111V → R in AAA49000 (PubMed:1533881).Curated1
Sequence conflicti513A → R in AAA49000 (PubMed:1533881).Curated1
Sequence conflicti513A → R in AAB58343 (PubMed:9233775).Curated1
Sequence conflicti696V → I in BAA02872 (PubMed:7916720).Curated1
Sequence conflicti708 – 710ADV → VDA in BAA02872 (PubMed:7916720).Curated3
Sequence conflicti774L → H in AAB58343 (PubMed:9233775).Curated1
Sequence conflicti848R → Q in AAA49000 (PubMed:1533881).Curated1
Sequence conflicti868V → G in AAA49000 (PubMed:1533881).Curated1
Sequence conflicti875L → F in AAA49000 (PubMed:1533881).Curated1
Sequence conflicti898 – 899SH → IY in BAA02872 (PubMed:7916720).Curated2
Sequence conflicti903N → K in AAA49000 (PubMed:1533881).Curated1
Sequence conflicti938S → T in AAB58343 (PubMed:9233775).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_042871970Y → YG in isoform 2. 2 Publications1
Alternative sequenceiVSP_042872971 – 983RKAQC…IYLTR → GQESSVQSSYIPN in isoform 3. 1 PublicationAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86930 mRNA Translation: AAA49000.1
D13719 mRNA Translation: BAA02872.1
AF000241 mRNA Translation: AAB58343.1 Sequence problems.
AADN02031451 Genomic DNA No translation available.
PIRiA41996
RefSeqiNP_990465.1, NM_205134.1
XP_015140901.1, XM_015285415.1 [Q04861-3]
UniGeneiGga.3531

Genome annotation databases

EnsembliENSGALT00000037607; ENSGALP00000036814; ENSGALG00000012304 [Q04861-3]
GeneIDi396033
KEGGigga:396033

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNFKB1_CHICK
AccessioniPrimary (citable) accession number: Q04861
Secondary accession number(s): E1C613, F1NU28, O13075
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: April 18, 2012
Last modified: May 23, 2018
This is version 153 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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