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Q04859

- MAK_MOUSE

UniProt

Q04859 - MAK_MOUSE

Protein

Serine/threonine-protein kinase MAK

Gene

Mak

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Essential for the regulation of ciliary length and required for the long-term survival of photoreceptors. Could have an important function in sensory cells and in spermatogenesis. May participate in signaling pathways used in visual and olfactory sensory transduction. Phosphorylates FZR1 in a cell cycle-dependent manner. Plays a role in the transcriptional coactivation of AR By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei33 – 331ATPPROSITE-ProRule annotation
    Active sitei125 – 1251Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
    3. protein binding Source: UniProtKB
    4. transcription coactivator activity Source: UniProtKB

    GO - Biological processi

    1. photoreceptor cell maintenance Source: UniProtKB
    2. protein autophosphorylation Source: UniProtKB
    3. protein phosphorylation Source: UniProtKB
    4. regulation of transcription, DNA-templated Source: UniProtKB-KW
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.22. 3474.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase MAK (EC:2.7.11.22)
    Alternative name(s):
    Male germ cell-associated kinase
    Protein kinase RCK
    Gene namesi
    Name:Mak
    Synonyms:Rck
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:96913. Mak.

    Subcellular locationi

    Nucleus By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cytoplasmcytoskeletonspindle By similarity. Midbody By similarity. Cell projectionciliumphotoreceptor outer segment 1 Publication. Photoreceptor inner segment By similarity
    Note: Localizes in both the connecting cilia and the outer segment axonemes.

    GO - Cellular componenti

    1. centrosome Source: UniProtKB
    2. cytoplasm Source: UniProtKB-KW
    3. midbody Source: UniProtKB
    4. mitotic spindle Source: UniProtKB
    5. nucleus Source: UniProtKB
    6. photoreceptor inner segment Source: UniProtKB
    7. photoreceptor outer segment Source: UniProtKB

    Keywords - Cellular componenti

    Cell projection, Cilium, Cytoplasm, Cytoskeleton, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Mice are mostly fertile, develop normally, and exhibit no gross abnormalities and spermatogenesis is intact. However, both sperm motility and litter size is reduced.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 622622Serine/threonine-protein kinase MAKPRO_0000086285Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei157 – 1571Phosphothreonine; by autocatalysisBy similarity
    Modified residuei159 – 1591Phosphotyrosine; by autocatalysisBy similarity

    Post-translational modificationi

    Autophosphorylated. Phosphorylated on serine and threonine residues By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ04859.
    PRIDEiQ04859.

    PTM databases

    PhosphoSiteiQ04859.

    Expressioni

    Tissue specificityi

    In pre- and postmeiotic male germ cells in testis. In photoreceptor cells of the retina and in the olfactory receptors, and in certain epithelia of the respiratory tract and choroid plexus (brain).1 Publication

    Developmental stagei

    On day 14 or 17 of embryonic development. Expression is observed in germ cells at the stages of late pachytene spermatocytes through to early round spermatids.1 Publication

    Gene expression databases

    BgeeiQ04859.
    CleanExiMM_MAK.
    GenevestigatoriQ04859.

    Interactioni

    Subunit structurei

    Interacts with AR and CDK20. Found in a complex containing MAK, AR and NCOA3. Interacts with FZR1 (via WD repeats) By similarity. Interacts with RP1.By similarity1 Publication

    Protein-protein interaction databases

    DIPiDIP-59494N.
    STRINGi10090.ENSMUSP00000021792.

    Structurei

    3D structure databases

    ProteinModelPortaliQ04859.
    SMRiQ04859. Positions 1-353.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 284281Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi309 – 36860Glu/Pro-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00650000093283.
    HOGENOMiHOG000233024.
    HOVERGENiHBG014652.
    InParanoidiQ04859.
    KOiK08829.
    OMAiYATYNQS.
    OrthoDBiEOG7NCV37.
    TreeFamiTF328769.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q04859-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MNRYTTMKQL GDGTYGSVLM GKSNESGELV AIKRMKRKFY SWDECMNLRE    50
    VKSLKKLNHA NVIKLKEVIR ENDHLYFVFE YMKENLYQLM KDRNKLFPES 100
    VIRNIMYQIL QGLAFIHKHG FFHRDMKPEN LLCMGPELVK IADFGLAREL 150
    RSQPPYTDYV STRWYRAPEV LLRSSVYSSP IDVWAVGSIM AELYTFRPLF 200
    PGTSEVDEIF KICQVLGTPK KSDWPEGYQL ASSMNFRFPQ CIPINLKTLI 250
    PNASSEAIQL MTEMLNWDPK KRPTASQALK HPYFQVGQVL GSSAHHLDTK 300
    QTLHKQLQPL EPKPSSSERD PKPLPNILDQ PAGQPQPKQG HQPLQTIQPP 350
    QNTVTHPPPK QQGHQKPPQT MFPSIIKTIP VNSVSTLGHK GARRRWGQTV 400
    FKSGDSCDDI EDDLGASHSK KPSMEACKEK KKESPFRFPD SGLPVSNHFK 450
    GENRNLHASV SLKSDPNLST ASTAKQYYLK QSRYLPGVNP KNVSLVAGGK 500
    DINSHSWNNQ LFPKSLGSMG ADLSFKRSNA AGNLGSYTTY NQTGYMPSFL 550
    KKEVGSAGQR IQLAPLGASA SDYTWSTKTG RGQFSGRTYN PTAKNLNIVN 600
    RTQPVPSVHG RTDWVAKYGG HR 622
    Length:622
    Mass (Da):70,080
    Last modified:July 27, 2011 - v2
    Checksum:iDB7E3B758F8FEA69
    GO
    Isoform 2 (identifier: Q04859-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         531-531: A → EDSIIKPIENLSCTGKSAEQLEDPQ

    Show »
    Length:646
    Mass (Da):72,736
    Checksum:i05CA06912663F2CC
    GO
    Isoform 3 (identifier: Q04859-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         94-124: Missing.
         531-571: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:550
    Mass (Da):62,125
    Checksum:i639D4B5A3A7465B0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti128 – 1281P → H in BAC26662. (PubMed:16141072)Curated
    Sequence conflicti235 – 2351N → D in BAC26662. (PubMed:16141072)Curated
    Sequence conflicti386 – 3861T → A in CAA47392. (PubMed:8359591)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei94 – 12431Missing in isoform 3. 1 PublicationVSP_042472Add
    BLAST
    Alternative sequencei531 – 57141Missing in isoform 3. 1 PublicationVSP_042473Add
    BLAST
    Alternative sequencei531 – 5311A → EDSIIKPIENLSCTGKSAEQ LEDPQ in isoform 2. CuratedVSP_042474

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X66983 mRNA. Translation: CAA47392.1.
    AK029894 mRNA. Translation: BAC26662.1.
    AC133496 Genomic DNA. No translation available.
    CCDSiCCDS26471.1. [Q04859-1]
    CCDS49244.1. [Q04859-3]
    CCDS49245.1. [Q04859-2]
    PIRiI48733.
    RefSeqiNP_001139274.1. NM_001145802.1. [Q04859-3]
    NP_001139275.1. NM_001145803.1. [Q04859-2]
    NP_032573.2. NM_008547.2. [Q04859-1]
    XP_006516934.1. XM_006516871.1. [Q04859-1]
    UniGeneiMm.8149.

    Genome annotation databases

    EnsembliENSMUST00000021792; ENSMUSP00000021792; ENSMUSG00000021363. [Q04859-1]
    ENSMUST00000070193; ENSMUSP00000064750; ENSMUSG00000021363. [Q04859-3]
    ENSMUST00000165087; ENSMUSP00000129615; ENSMUSG00000021363. [Q04859-2]
    GeneIDi17152.
    KEGGimmu:17152.
    UCSCiuc007qev.2. mouse. [Q04859-1]
    uc007qew.2. mouse. [Q04859-2]
    uc007qex.2. mouse. [Q04859-3]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X66983 mRNA. Translation: CAA47392.1 .
    AK029894 mRNA. Translation: BAC26662.1 .
    AC133496 Genomic DNA. No translation available.
    CCDSi CCDS26471.1. [Q04859-1 ]
    CCDS49244.1. [Q04859-3 ]
    CCDS49245.1. [Q04859-2 ]
    PIRi I48733.
    RefSeqi NP_001139274.1. NM_001145802.1. [Q04859-3 ]
    NP_001139275.1. NM_001145803.1. [Q04859-2 ]
    NP_032573.2. NM_008547.2. [Q04859-1 ]
    XP_006516934.1. XM_006516871.1. [Q04859-1 ]
    UniGenei Mm.8149.

    3D structure databases

    ProteinModelPortali Q04859.
    SMRi Q04859. Positions 1-353.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-59494N.
    STRINGi 10090.ENSMUSP00000021792.

    Chemistry

    ChEMBLi CHEMBL2176784.

    PTM databases

    PhosphoSitei Q04859.

    Proteomic databases

    PaxDbi Q04859.
    PRIDEi Q04859.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000021792 ; ENSMUSP00000021792 ; ENSMUSG00000021363 . [Q04859-1 ]
    ENSMUST00000070193 ; ENSMUSP00000064750 ; ENSMUSG00000021363 . [Q04859-3 ]
    ENSMUST00000165087 ; ENSMUSP00000129615 ; ENSMUSG00000021363 . [Q04859-2 ]
    GeneIDi 17152.
    KEGGi mmu:17152.
    UCSCi uc007qev.2. mouse. [Q04859-1 ]
    uc007qew.2. mouse. [Q04859-2 ]
    uc007qex.2. mouse. [Q04859-3 ]

    Organism-specific databases

    CTDi 4117.
    MGIi MGI:96913. Mak.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00650000093283.
    HOGENOMi HOG000233024.
    HOVERGENi HBG014652.
    InParanoidi Q04859.
    KOi K08829.
    OMAi YATYNQS.
    OrthoDBi EOG7NCV37.
    TreeFami TF328769.

    Enzyme and pathway databases

    BRENDAi 2.7.11.22. 3474.

    Miscellaneous databases

    NextBioi 291410.
    PROi Q04859.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q04859.
    CleanExi MM_MAK.
    Genevestigatori Q04859.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization and expression analysis of the murine rck gene: a protein kinase with a potential function in sensory cells."
      Bladt F., Birchmeier C.
      Differentiation 53:115-122(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
      Strain: C57BL/6J.
      Tissue: Testis.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "In situ localization of male germ cell-associated kinase (mak) mRNA in adult mouse testis: specific expression in germ cells at stages around meiotic cell division."
      Koji T., Jinno A., Matsushime H., Shibuya M., Nakane P.K.
      Cell Biochem. Funct. 10:273-279(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
    5. "A testicular germ cell-associated serine-threonine kinase, MAK, is dispensable for sperm formation."
      Shinkai Y., Satoh H., Takeda N., Fukuda M., Chiba E., Kato T., Kuramochi T., Araki Y.
      Mol. Cell. Biol. 22:3276-3280(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    6. "Negative regulation of ciliary length by ciliary male germ cell-associated kinase (Mak) is required for retinal photoreceptor survival."
      Omori Y., Chaya T., Katoh K., Kajimura N., Sato S., Muraoka K., Ueno S., Koyasu T., Kondo M., Furukawa T.
      Proc. Natl. Acad. Sci. U.S.A. 107:22671-22676(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH RP1, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiMAK_MOUSE
    AccessioniPrimary (citable) accession number: Q04859
    Secondary accession number(s): E9Q4B2
    , E9QAU5, E9QKR3, Q8CDL5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3