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Q04859

- MAK_MOUSE

UniProt

Q04859 - MAK_MOUSE

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Protein

Serine/threonine-protein kinase MAK

Gene

Mak

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Essential for the regulation of ciliary length and required for the long-term survival of photoreceptors. Could have an important function in sensory cells and in spermatogenesis. May participate in signaling pathways used in visual and olfactory sensory transduction. Phosphorylates FZR1 in a cell cycle-dependent manner. Plays a role in the transcriptional coactivation of AR (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331ATPPROSITE-ProRule annotation
Active sitei125 – 1251Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
  3. transcription coactivator activity Source: UniProtKB

GO - Biological processi

  1. photoreceptor cell maintenance Source: UniProtKB
  2. protein autophosphorylation Source: UniProtKB
  3. protein phosphorylation Source: UniProtKB
  4. regulation of transcription, DNA-templated Source: UniProtKB-KW
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.22. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase MAK (EC:2.7.11.22)
Alternative name(s):
Male germ cell-associated kinase
Protein kinase RCK
Gene namesi
Name:Mak
Synonyms:Rck
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 13

Organism-specific databases

MGIiMGI:96913. Mak.

Subcellular locationi

Nucleus By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cytoplasmcytoskeletonspindle By similarity. Midbody By similarity. Cell projectionciliumphotoreceptor outer segment 1 Publication. Photoreceptor inner segment By similarity
Note: Localizes in both the connecting cilia and the outer segment axonemes.

GO - Cellular componenti

  1. centrosome Source: UniProtKB
  2. cytoplasm Source: UniProtKB-KW
  3. midbody Source: UniProtKB
  4. mitotic spindle Source: UniProtKB
  5. nucleus Source: UniProtKB
  6. photoreceptor inner segment Source: UniProtKB
  7. photoreceptor outer segment Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice are mostly fertile, develop normally, and exhibit no gross abnormalities and spermatogenesis is intact. However, both sperm motility and litter size is reduced.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 622622Serine/threonine-protein kinase MAKPRO_0000086285Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei157 – 1571Phosphothreonine; by autocatalysisBy similarity
Modified residuei159 – 1591Phosphotyrosine; by autocatalysisBy similarity

Post-translational modificationi

Autophosphorylated. Phosphorylated on serine and threonine residues (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ04859.
PRIDEiQ04859.

PTM databases

PhosphoSiteiQ04859.

Expressioni

Tissue specificityi

In pre- and postmeiotic male germ cells in testis. In photoreceptor cells of the retina and in the olfactory receptors, and in certain epithelia of the respiratory tract and choroid plexus (brain).1 Publication

Developmental stagei

On day 14 or 17 of embryonic development. Expression is observed in germ cells at the stages of late pachytene spermatocytes through to early round spermatids.1 Publication

Gene expression databases

BgeeiQ04859.
CleanExiMM_MAK.
GenevestigatoriQ04859.

Interactioni

Subunit structurei

Interacts with AR and CDK20. Found in a complex containing MAK, AR and NCOA3. Interacts with FZR1 (via WD repeats) (By similarity). Interacts with RP1.By similarity1 Publication

Protein-protein interaction databases

DIPiDIP-59494N.
STRINGi10090.ENSMUSP00000021792.

Structurei

3D structure databases

ProteinModelPortaliQ04859.
SMRiQ04859. Positions 1-353.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 284281Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi309 – 36860Glu/Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00650000093283.
HOGENOMiHOG000233024.
HOVERGENiHBG014652.
InParanoidiQ04859.
KOiK08829.
OMAiYATYNQS.
OrthoDBiEOG7NCV37.
TreeFamiTF328769.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q04859-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNRYTTMKQL GDGTYGSVLM GKSNESGELV AIKRMKRKFY SWDECMNLRE
60 70 80 90 100
VKSLKKLNHA NVIKLKEVIR ENDHLYFVFE YMKENLYQLM KDRNKLFPES
110 120 130 140 150
VIRNIMYQIL QGLAFIHKHG FFHRDMKPEN LLCMGPELVK IADFGLAREL
160 170 180 190 200
RSQPPYTDYV STRWYRAPEV LLRSSVYSSP IDVWAVGSIM AELYTFRPLF
210 220 230 240 250
PGTSEVDEIF KICQVLGTPK KSDWPEGYQL ASSMNFRFPQ CIPINLKTLI
260 270 280 290 300
PNASSEAIQL MTEMLNWDPK KRPTASQALK HPYFQVGQVL GSSAHHLDTK
310 320 330 340 350
QTLHKQLQPL EPKPSSSERD PKPLPNILDQ PAGQPQPKQG HQPLQTIQPP
360 370 380 390 400
QNTVTHPPPK QQGHQKPPQT MFPSIIKTIP VNSVSTLGHK GARRRWGQTV
410 420 430 440 450
FKSGDSCDDI EDDLGASHSK KPSMEACKEK KKESPFRFPD SGLPVSNHFK
460 470 480 490 500
GENRNLHASV SLKSDPNLST ASTAKQYYLK QSRYLPGVNP KNVSLVAGGK
510 520 530 540 550
DINSHSWNNQ LFPKSLGSMG ADLSFKRSNA AGNLGSYTTY NQTGYMPSFL
560 570 580 590 600
KKEVGSAGQR IQLAPLGASA SDYTWSTKTG RGQFSGRTYN PTAKNLNIVN
610 620
RTQPVPSVHG RTDWVAKYGG HR
Length:622
Mass (Da):70,080
Last modified:July 27, 2011 - v2
Checksum:iDB7E3B758F8FEA69
GO
Isoform 2 (identifier: Q04859-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     531-531: A → EDSIIKPIENLSCTGKSAEQLEDPQ

Show »
Length:646
Mass (Da):72,736
Checksum:i05CA06912663F2CC
GO
Isoform 3 (identifier: Q04859-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     94-124: Missing.
     531-571: Missing.

Note: No experimental confirmation available.

Show »
Length:550
Mass (Da):62,125
Checksum:i639D4B5A3A7465B0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti128 – 1281P → H in BAC26662. (PubMed:16141072)Curated
Sequence conflicti235 – 2351N → D in BAC26662. (PubMed:16141072)Curated
Sequence conflicti386 – 3861T → A in CAA47392. (PubMed:8359591)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei94 – 12431Missing in isoform 3. 1 PublicationVSP_042472Add
BLAST
Alternative sequencei531 – 57141Missing in isoform 3. 1 PublicationVSP_042473Add
BLAST
Alternative sequencei531 – 5311A → EDSIIKPIENLSCTGKSAEQ LEDPQ in isoform 2. CuratedVSP_042474

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66983 mRNA. Translation: CAA47392.1.
AK029894 mRNA. Translation: BAC26662.1.
AC133496 Genomic DNA. No translation available.
CCDSiCCDS26471.1. [Q04859-1]
CCDS49244.1. [Q04859-3]
CCDS49245.1. [Q04859-2]
PIRiI48733.
RefSeqiNP_001139274.1. NM_001145802.1. [Q04859-3]
NP_001139275.1. NM_001145803.1. [Q04859-2]
NP_032573.2. NM_008547.2. [Q04859-1]
XP_006516934.1. XM_006516871.1. [Q04859-1]
UniGeneiMm.8149.

Genome annotation databases

EnsembliENSMUST00000021792; ENSMUSP00000021792; ENSMUSG00000021363. [Q04859-1]
ENSMUST00000070193; ENSMUSP00000064750; ENSMUSG00000021363. [Q04859-3]
ENSMUST00000165087; ENSMUSP00000129615; ENSMUSG00000021363. [Q04859-2]
GeneIDi17152.
KEGGimmu:17152.
UCSCiuc007qev.2. mouse. [Q04859-1]
uc007qew.2. mouse. [Q04859-2]
uc007qex.2. mouse. [Q04859-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66983 mRNA. Translation: CAA47392.1 .
AK029894 mRNA. Translation: BAC26662.1 .
AC133496 Genomic DNA. No translation available.
CCDSi CCDS26471.1. [Q04859-1 ]
CCDS49244.1. [Q04859-3 ]
CCDS49245.1. [Q04859-2 ]
PIRi I48733.
RefSeqi NP_001139274.1. NM_001145802.1. [Q04859-3 ]
NP_001139275.1. NM_001145803.1. [Q04859-2 ]
NP_032573.2. NM_008547.2. [Q04859-1 ]
XP_006516934.1. XM_006516871.1. [Q04859-1 ]
UniGenei Mm.8149.

3D structure databases

ProteinModelPortali Q04859.
SMRi Q04859. Positions 1-353.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-59494N.
STRINGi 10090.ENSMUSP00000021792.

Chemistry

ChEMBLi CHEMBL2176784.

PTM databases

PhosphoSitei Q04859.

Proteomic databases

PaxDbi Q04859.
PRIDEi Q04859.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000021792 ; ENSMUSP00000021792 ; ENSMUSG00000021363 . [Q04859-1 ]
ENSMUST00000070193 ; ENSMUSP00000064750 ; ENSMUSG00000021363 . [Q04859-3 ]
ENSMUST00000165087 ; ENSMUSP00000129615 ; ENSMUSG00000021363 . [Q04859-2 ]
GeneIDi 17152.
KEGGi mmu:17152.
UCSCi uc007qev.2. mouse. [Q04859-1 ]
uc007qew.2. mouse. [Q04859-2 ]
uc007qex.2. mouse. [Q04859-3 ]

Organism-specific databases

CTDi 4117.
MGIi MGI:96913. Mak.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00650000093283.
HOGENOMi HOG000233024.
HOVERGENi HBG014652.
InParanoidi Q04859.
KOi K08829.
OMAi YATYNQS.
OrthoDBi EOG7NCV37.
TreeFami TF328769.

Enzyme and pathway databases

BRENDAi 2.7.11.22. 3474.

Miscellaneous databases

NextBioi 291410.
PROi Q04859.
SOURCEi Search...

Gene expression databases

Bgeei Q04859.
CleanExi MM_MAK.
Genevestigatori Q04859.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization and expression analysis of the murine rck gene: a protein kinase with a potential function in sensory cells."
    Bladt F., Birchmeier C.
    Differentiation 53:115-122(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: C57BL/6J.
    Tissue: Testis.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "In situ localization of male germ cell-associated kinase (mak) mRNA in adult mouse testis: specific expression in germ cells at stages around meiotic cell division."
    Koji T., Jinno A., Matsushime H., Shibuya M., Nakane P.K.
    Cell Biochem. Funct. 10:273-279(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  5. "A testicular germ cell-associated serine-threonine kinase, MAK, is dispensable for sperm formation."
    Shinkai Y., Satoh H., Takeda N., Fukuda M., Chiba E., Kato T., Kuramochi T., Araki Y.
    Mol. Cell. Biol. 22:3276-3280(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  6. "Negative regulation of ciliary length by ciliary male germ cell-associated kinase (Mak) is required for retinal photoreceptor survival."
    Omori Y., Chaya T., Katoh K., Kajimura N., Sato S., Muraoka K., Ueno S., Koyasu T., Kondo M., Furukawa T.
    Proc. Natl. Acad. Sci. U.S.A. 107:22671-22676(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH RP1, TISSUE SPECIFICITY.

Entry informationi

Entry nameiMAK_MOUSE
AccessioniPrimary (citable) accession number: Q04859
Secondary accession number(s): E9Q4B2
, E9QAU5, E9QKR3, Q8CDL5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3