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Q04859 (MAK_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase MAK

EC=2.7.11.22
Alternative name(s):
Male germ cell-associated kinase
Protein kinase RCK
Gene names
Name:Mak
Synonyms:Rck
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length622 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential for the regulation of ciliary length and required for the long-term survival of photoreceptors. Could have an important function in sensory cells and in spermatogenesis. May participate in signaling pathways used in visual and olfactory sensory transduction. Phosphorylates FZR1 in a cell cycle-dependent manner. Plays a role in the transcriptional coactivation of AR By similarity. Ref.6

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Interacts with AR and CDK20. Found in a complex containing MAK, AR and NCOA3. Interacts with FZR1 (via WD repeats) By similarity. Interacts with RP1. Ref.6

Subcellular location

Nucleus By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cytoplasmcytoskeletonspindle By similarity. Midbody By similarity. Cell projectionciliumphotoreceptor outer segment. Photoreceptor inner segment By similarity. Note: Localizes in both the connecting cilia and the outer segment axonemes. Ref.6

Tissue specificity

In pre- and postmeiotic male germ cells in testis. In photoreceptor cells of the retina and in the olfactory receptors, and in certain epithelia of the respiratory tract and choroid plexus (brain). Ref.6

Developmental stage

On day 14 or 17 of embryonic development. Expression is observed in germ cells at the stages of late pachytene spermatocytes through to early round spermatids. Ref.4

Post-translational modification

Autophosphorylated. Phosphorylated on serine and threonine residues By similarity.

Disruption phenotype

Mice are mostly fertile, develop normally, and exhibit no gross abnormalities and spermatogenesis is intact. However, both sperm motility and litter size is reduced. Ref.5

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentCell projection
Cilium
Cytoplasm
Cytoskeleton
Nucleus
   Coding sequence diversityAlternative splicing
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processphotoreceptor cell maintenance

Inferred from mutant phenotype Ref.6. Source: UniProtKB

protein autophosphorylation

Inferred from sequence or structural similarity. Source: UniProtKB

protein phosphorylation

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcentrosome

Inferred from sequence or structural similarity. Source: UniProtKB

cytoplasm

Inferred from electronic annotation. Source: UniProtKB-KW

midbody

Inferred from sequence or structural similarity. Source: UniProtKB

mitotic spindle

Inferred from sequence or structural similarity. Source: UniProtKB

nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

photoreceptor inner segment

Inferred from sequence or structural similarity. Source: UniProtKB

photoreceptor outer segment

Inferred from direct assay Ref.6. Source: UniProtKB

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

cyclin-dependent protein serine/threonine kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

transcription coactivator activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q04859-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q04859-2)

The sequence of this isoform differs from the canonical sequence as follows:
     531-531: A → EDSIIKPIENLSCTGKSAEQLEDPQ
Isoform 3 (identifier: Q04859-3)

The sequence of this isoform differs from the canonical sequence as follows:
     94-124: Missing.
     531-571: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 622622Serine/threonine-protein kinase MAK
PRO_0000086285

Regions

Domain4 – 284281Protein kinase
Nucleotide binding10 – 189ATP By similarity
Compositional bias309 – 36860Glu/Pro-rich

Sites

Active site1251Proton acceptor By similarity
Binding site331ATP By similarity

Amino acid modifications

Modified residue1571Phosphothreonine; by autocatalysis By similarity
Modified residue1591Phosphotyrosine; by autocatalysis By similarity

Natural variations

Alternative sequence94 – 12431Missing in isoform 3.
VSP_042472
Alternative sequence531 – 57141Missing in isoform 3.
VSP_042473
Alternative sequence5311A → EDSIIKPIENLSCTGKSAEQ LEDPQ in isoform 2.
VSP_042474

Experimental info

Sequence conflict1281P → H in BAC26662. Ref.2
Sequence conflict2351N → D in BAC26662. Ref.2
Sequence conflict3861T → A in CAA47392. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: DB7E3B758F8FEA69

FASTA62270,080
        10         20         30         40         50         60 
MNRYTTMKQL GDGTYGSVLM GKSNESGELV AIKRMKRKFY SWDECMNLRE VKSLKKLNHA 

        70         80         90        100        110        120 
NVIKLKEVIR ENDHLYFVFE YMKENLYQLM KDRNKLFPES VIRNIMYQIL QGLAFIHKHG 

       130        140        150        160        170        180 
FFHRDMKPEN LLCMGPELVK IADFGLAREL RSQPPYTDYV STRWYRAPEV LLRSSVYSSP 

       190        200        210        220        230        240 
IDVWAVGSIM AELYTFRPLF PGTSEVDEIF KICQVLGTPK KSDWPEGYQL ASSMNFRFPQ 

       250        260        270        280        290        300 
CIPINLKTLI PNASSEAIQL MTEMLNWDPK KRPTASQALK HPYFQVGQVL GSSAHHLDTK 

       310        320        330        340        350        360 
QTLHKQLQPL EPKPSSSERD PKPLPNILDQ PAGQPQPKQG HQPLQTIQPP QNTVTHPPPK 

       370        380        390        400        410        420 
QQGHQKPPQT MFPSIIKTIP VNSVSTLGHK GARRRWGQTV FKSGDSCDDI EDDLGASHSK 

       430        440        450        460        470        480 
KPSMEACKEK KKESPFRFPD SGLPVSNHFK GENRNLHASV SLKSDPNLST ASTAKQYYLK 

       490        500        510        520        530        540 
QSRYLPGVNP KNVSLVAGGK DINSHSWNNQ LFPKSLGSMG ADLSFKRSNA AGNLGSYTTY 

       550        560        570        580        590        600 
NQTGYMPSFL KKEVGSAGQR IQLAPLGASA SDYTWSTKTG RGQFSGRTYN PTAKNLNIVN 

       610        620 
RTQPVPSVHG RTDWVAKYGG HR 

« Hide

Isoform 2 [UniParc].

Checksum: 05CA06912663F2CC
Show »

FASTA64672,736
Isoform 3 [UniParc].

Checksum: 639D4B5A3A7465B0
Show »

FASTA55062,125

References

« Hide 'large scale' references
[1]"Characterization and expression analysis of the murine rck gene: a protein kinase with a potential function in sensory cells."
Bladt F., Birchmeier C.
Differentiation 53:115-122(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Strain: C57BL/6J.
Tissue: Testis.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"In situ localization of male germ cell-associated kinase (mak) mRNA in adult mouse testis: specific expression in germ cells at stages around meiotic cell division."
Koji T., Jinno A., Matsushime H., Shibuya M., Nakane P.K.
Cell Biochem. Funct. 10:273-279(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
[5]"A testicular germ cell-associated serine-threonine kinase, MAK, is dispensable for sperm formation."
Shinkai Y., Satoh H., Takeda N., Fukuda M., Chiba E., Kato T., Kuramochi T., Araki Y.
Mol. Cell. Biol. 22:3276-3280(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
[6]"Negative regulation of ciliary length by ciliary male germ cell-associated kinase (Mak) is required for retinal photoreceptor survival."
Omori Y., Chaya T., Katoh K., Kajimura N., Sato S., Muraoka K., Ueno S., Koyasu T., Kondo M., Furukawa T.
Proc. Natl. Acad. Sci. U.S.A. 107:22671-22676(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH RP1, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X66983 mRNA. Translation: CAA47392.1.
AK029894 mRNA. Translation: BAC26662.1.
AC133496 Genomic DNA. No translation available.
PIRI48733.
RefSeqNP_001139274.1. NM_001145802.1.
NP_001139275.1. NM_001145803.1.
NP_032573.2. NM_008547.2.
XP_006516934.1. XM_006516871.1.
UniGeneMm.8149.

3D structure databases

ProteinModelPortalQ04859.
SMRQ04859. Positions 1-353.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-59494N.
STRING10090.ENSMUSP00000021792.

Chemistry

ChEMBLCHEMBL2176784.

PTM databases

PhosphoSiteQ04859.

Proteomic databases

PaxDbQ04859.
PRIDEQ04859.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000021792; ENSMUSP00000021792; ENSMUSG00000021363. [Q04859-1]
ENSMUST00000070193; ENSMUSP00000064750; ENSMUSG00000021363. [Q04859-3]
ENSMUST00000165087; ENSMUSP00000129615; ENSMUSG00000021363. [Q04859-2]
GeneID17152.
KEGGmmu:17152.
UCSCuc007qev.2. mouse. [Q04859-1]
uc007qew.2. mouse. [Q04859-2]
uc007qex.2. mouse. [Q04859-3]

Organism-specific databases

CTD4117.
MGIMGI:96913. Mak.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00650000093283.
HOGENOMHOG000233024.
HOVERGENHBG014652.
InParanoidQ04859.
KOK08829.
OMAYATYNQS.
OrthoDBEOG7NCV37.
TreeFamTF328769.

Enzyme and pathway databases

BRENDA2.7.11.22. 3474.

Gene expression databases

BgeeQ04859.
CleanExMM_MAK.
GenevestigatorQ04859.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio291410.
PROQ04859.
SOURCESearch...

Entry information

Entry nameMAK_MOUSE
AccessionPrimary (citable) accession number: Q04859
Secondary accession number(s): E9Q4B2 expand/collapse secondary AC list , E9QAU5, E9QKR3, Q8CDL5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot