Reviewed, UniProtKB/Swiss-Prot Q04852 (T2C1_CITFR)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme CfrBI
      Short name=R.CfrBI
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme CfrBI
    Endonuclease CfrBI

Gene names

Name:

cfrBIR

Encoded on

Plasmid pZE8

Organism

Citrobacter freundii

Taxonomic identifier

546 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Citrobacter

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Protein existence	Predicted.

General annotation (Comments)

Function	Recognizes the double-stranded sequence CCWWGG and cleaves after C-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Plasmid
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	Type-2 restriction enzyme CfrBI		PRO_0000077296

Sequences

	Sequence		Length	Mass (Da)
	Q04852-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 0FDF4014F95C496A	FASTA	355	40,413
	10 20 30 40 50 60 MNFKDKNCFP NELIALAKIS KNDVLDKFGT DVFKKVVYDV LTGKNVREFT EILTRTRLLE 70 80 90 100 110 120 SNLSFFDFFV DKMKEGITPK QLYLYAKNAL SNKSYVKSNQ PVLEWMVMMT NKQTQNVLRD 130 140 150 160 170 180 EHGDGFDRLA LRTQEEILKI KNGYEDKIGE ISIGGQKVSL EDFCYIILSL GSQTLTIRGS 190 200 210 220 230 240 EKSLHGKYFE KLILGSLFTI MGFEYKEKIE EGLNAKCFTL STRADDRESD ATLVFNGKAI 250 260 270 280 290 300 RVDIGFIGRG NTEISLDKVS RFRRMDDIGG VMHNISTMVI VDVIGDRSRI VNMAEEIDGK 310 320 330 340 350 VVAMSDPYWV AKVSSYISSK LNVDDLLEDK PQLKDIQSFI SDALENVDLE KYIKL

References

[1]	"Cloning and sequences of the genes encoding the CfrBI restriction-modification system from Citrobacter freundii." Zakharova M.V., Kravetz A.N., Beletzkaja I.V., Repyk A.V., Solonin A.S. Gene 129:77-81(1993) [PubMed: 8335262] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 4111.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X57945 Genomic DNA. Translation: CAA41013.1.
PIR	JN0746. S28523.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	2422. CfrBI.
Enzyme and pathway databases
BRENDA	3.1.21.4. 2347.
Family and domain databases
InterPro	IPR019042. Restrict_endonuc_II_CfrBI. [Graphical view]
Pfam	PF09516. RE_CfrBI. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2C1_CITFR

Accession

Primary (citable) accession number: Q04852

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)