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Q04852

- T2C1_CITFR

UniProt

Q04852 - T2C1_CITFR

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Protein

Type-2 restriction enzyme CfrBI

Gene

cfrBIR

Organism
Citrobacter freundii
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi

Functioni

Recognizes the double-stranded sequence CCWWGG and cleaves after C-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi2422. CfrBI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme CfrBI (EC:3.1.21.4)
Short name:
R.CfrBI
Alternative name(s):
Endonuclease CfrBI
Type II restriction enzyme CfrBI
Gene namesi
Name:cfrBIR
Encoded oniPlasmid pZE80 Publication
OrganismiCitrobacter freundii
Taxonomic identifieri546 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCitrobacterCitrobacter freundii complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 355355Type-2 restriction enzyme CfrBIPRO_0000077296Add
BLAST

Family & Domainsi

Family and domain databases

InterProiIPR019042. Restrct_endonuc_II_CfrBI.
[Graphical view]
PfamiPF09516. RE_CfrBI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04852-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MNFKDKNCFP NELIALAKIS KNDVLDKFGT DVFKKVVYDV LTGKNVREFT
60 70 80 90 100
EILTRTRLLE SNLSFFDFFV DKMKEGITPK QLYLYAKNAL SNKSYVKSNQ
110 120 130 140 150
PVLEWMVMMT NKQTQNVLRD EHGDGFDRLA LRTQEEILKI KNGYEDKIGE
160 170 180 190 200
ISIGGQKVSL EDFCYIILSL GSQTLTIRGS EKSLHGKYFE KLILGSLFTI
210 220 230 240 250
MGFEYKEKIE EGLNAKCFTL STRADDRESD ATLVFNGKAI RVDIGFIGRG
260 270 280 290 300
NTEISLDKVS RFRRMDDIGG VMHNISTMVI VDVIGDRSRI VNMAEEIDGK
310 320 330 340 350
VVAMSDPYWV AKVSSYISSK LNVDDLLEDK PQLKDIQSFI SDALENVDLE

KYIKL
Length:355
Mass (Da):40,413
Last modified:February 1, 1994 - v1
Checksum:i0FDF4014F95C496A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57945 Genomic DNA. Translation: CAA41013.1.
PIRiJN0746.
S28523.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57945 Genomic DNA. Translation: CAA41013.1 .
PIRi JN0746.
S28523.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 2422. CfrBI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR019042. Restrct_endonuc_II_CfrBI.
[Graphical view ]
Pfami PF09516. RE_CfrBI. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequences of the genes encoding the CfrBI restriction-modification system from Citrobacter freundii."
    Zakharova M.V., Kravetz A.N., Beletzkaja I.V., Repyk A.V., Solonin A.S.
    Gene 129:77-81(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 4111.

Entry informationi

Entry nameiT2C1_CITFR
AccessioniPrimary (citable) accession number: Q04852
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3