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Q04852

- T2C1_CITFR

UniProt

Q04852 - T2C1_CITFR

Protein

Type-2 restriction enzyme CfrBI

Gene

cfrBIR

Organism
Citrobacter freundii
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi
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    • History
      Entry version 46 (16 Apr 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence CCWWGG and cleaves after C-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi2422. CfrBI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme CfrBI (EC:3.1.21.4)
    Short name:
    R.CfrBI
    Alternative name(s):
    Endonuclease CfrBI
    Type II restriction enzyme CfrBI
    Gene namesi
    Name:cfrBIR
    Encoded oniPlasmid pZE80 Publication
    OrganismiCitrobacter freundii
    Taxonomic identifieri546 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCitrobacterCitrobacter freundii complex

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 355355Type-2 restriction enzyme CfrBIPRO_0000077296Add
    BLAST

    Family & Domainsi

    Family and domain databases

    InterProiIPR019042. Restrct_endonuc_II_CfrBI.
    [Graphical view]
    PfamiPF09516. RE_CfrBI. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q04852-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNFKDKNCFP NELIALAKIS KNDVLDKFGT DVFKKVVYDV LTGKNVREFT    50
    EILTRTRLLE SNLSFFDFFV DKMKEGITPK QLYLYAKNAL SNKSYVKSNQ 100
    PVLEWMVMMT NKQTQNVLRD EHGDGFDRLA LRTQEEILKI KNGYEDKIGE 150
    ISIGGQKVSL EDFCYIILSL GSQTLTIRGS EKSLHGKYFE KLILGSLFTI 200
    MGFEYKEKIE EGLNAKCFTL STRADDRESD ATLVFNGKAI RVDIGFIGRG 250
    NTEISLDKVS RFRRMDDIGG VMHNISTMVI VDVIGDRSRI VNMAEEIDGK 300
    VVAMSDPYWV AKVSSYISSK LNVDDLLEDK PQLKDIQSFI SDALENVDLE 350
    KYIKL 355
    Length:355
    Mass (Da):40,413
    Last modified:February 1, 1994 - v1
    Checksum:i0FDF4014F95C496A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X57945 Genomic DNA. Translation: CAA41013.1.
    PIRiJN0746.
    S28523.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X57945 Genomic DNA. Translation: CAA41013.1 .
    PIRi JN0746.
    S28523.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 2422. CfrBI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR019042. Restrct_endonuc_II_CfrBI.
    [Graphical view ]
    Pfami PF09516. RE_CfrBI. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequences of the genes encoding the CfrBI restriction-modification system from Citrobacter freundii."
      Zakharova M.V., Kravetz A.N., Beletzkaja I.V., Repyk A.V., Solonin A.S.
      Gene 129:77-81(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 4111.

    Entry informationi

    Entry nameiT2C1_CITFR
    AccessioniPrimary (citable) accession number: Q04852
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: April 16, 2014
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Plasmid

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3