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Q04852 (T2C1_CITFR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme CfrBI

Short name=R.CfrBI
EC=3.1.21.4
Alternative name(s):
Endonuclease CfrBI
Type II restriction enzyme CfrBI
Gene names
Name:cfrBIR
Encoded onPlasmid pZE8
OrganismCitrobacter freundii
Taxonomic identifier546 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCitrobacterCitrobacter freundii complex

Protein attributes

Sequence length355 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence CCWWGG and cleaves after C-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termPlasmid
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 355355Type-2 restriction enzyme CfrBI
PRO_0000077296

Sequences

Sequence LengthMass (Da)Tools
Q04852 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 0FDF4014F95C496A

FASTA35540,413
        10         20         30         40         50         60 
MNFKDKNCFP NELIALAKIS KNDVLDKFGT DVFKKVVYDV LTGKNVREFT EILTRTRLLE 

        70         80         90        100        110        120 
SNLSFFDFFV DKMKEGITPK QLYLYAKNAL SNKSYVKSNQ PVLEWMVMMT NKQTQNVLRD 

       130        140        150        160        170        180 
EHGDGFDRLA LRTQEEILKI KNGYEDKIGE ISIGGQKVSL EDFCYIILSL GSQTLTIRGS 

       190        200        210        220        230        240 
EKSLHGKYFE KLILGSLFTI MGFEYKEKIE EGLNAKCFTL STRADDRESD ATLVFNGKAI 

       250        260        270        280        290        300 
RVDIGFIGRG NTEISLDKVS RFRRMDDIGG VMHNISTMVI VDVIGDRSRI VNMAEEIDGK 

       310        320        330        340        350 
VVAMSDPYWV AKVSSYISSK LNVDDLLEDK PQLKDIQSFI SDALENVDLE KYIKL 

« Hide

References

[1]"Cloning and sequences of the genes encoding the CfrBI restriction-modification system from Citrobacter freundii."
Zakharova M.V., Kravetz A.N., Beletzkaja I.V., Repyk A.V., Solonin A.S.
Gene 129:77-81(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 4111.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X57945 Genomic DNA. Translation: CAA41013.1.
PIRJN0746.
S28523.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE2422. CfrBI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR019042. Restrct_endonuc_II_CfrBI.
[Graphical view]
PfamPF09516. RE_CfrBI. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2C1_CITFR
AccessionPrimary (citable) accession number: Q04852
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries