Reviewed, UniProtKB/Swiss-Prot Q04845 (MTC1_CITFR)

Names and origin

Protein names

Recommended name:
    Modification methylase CfrBI
      Short name=M.CfrBI
    EC=2.1.1.113
Alternative name(s):
    N(4)- cytosine-specific methyltransferase CfrBI

Gene names

Name:

cfrBIM

Encoded on

Plasmid pZE8

Organism

Citrobacter freundii

Taxonomic identifier

546 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Citrobacter

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CCWWGG, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the CfrBI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	DNA methylation on cytosine Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (cytosine-N4-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Modification methylase CfrBI		PRO_0000087926

Sequences

	Sequence		Length	Mass (Da)
	Q04845-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 2EE9E08E36616A21	FASTA	376	43,358
	10 20 30 40 50 60 MTVKNNYLNR GYLVTSTTQA KKIAKLHLEK LDLADKFSFG LPEVDDRYHV WRVPLVATGH 70 80 90 100 110 120 RIGEIVINSK TSTIDYKKSS KSPFLIKKAM EVVHTPKKVK KEKKIISKSP LNNMLLQGNC 130 140 150 160 170 180 AETLKKLPDE SVNLVFTSPP YYNAKPEYSE YHTYDEYLSL LRSVIKECHR VLSEGRFFVI 190 200 210 220 230 240 NVSPVLIRRA SRNEASKRIA VPFDLHRLFI EEGYEFIDDI HWVKPEGAGW ALGRGRRFAA 250 260 270 280 290 300 DRNPLQYKPV PVTEYILVYR KKTDKLIDWN IRNHHSKEDV FDSKIGDDYE KTNLWKINPS 310 320 330 340 350 360 RNRKHPATFP YGLAERVIKY YSFKNDVILD PFAGSGTTAK AAIDLGRRFV MCEISKQYID 370 LIIEGTMLGG DLKIIN

References

[1]	"Cloning and sequences of the genes encoding the CfrBI restriction-modification system from Citrobacter freundii." Zakharova M.V., Kravetz A.N., Beletzkaja I.V., Repyk A.V., Solonin A.S. Gene 129:77-81(1993) [PubMed: 8335262] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 4111.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X57945 Genomic DNA. Translation: CAA41012.1.
PIR	JN0745.
3D structure databases
HSSP	HSSP built from PDB template 1G60 based on UniProtKB P23192.
ModBase	Search...
Protein family/group databases
REBASE	3548. M.CfrBI.
Enzyme and pathway databases
BRENDA	2.1.1.113. 2347.
Family and domain databases
InterPro	IPR002941. DNA_methylase_N4/N6. IPR001091. MeTrfase_CN4. IPR017985. MeTrfase_CN4_CS. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00508. S21N4MTFRASE.
PROSITE	PS00093. N4_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTC1_CITFR

Accession

Primary (citable) accession number: Q04845

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)