Q04841 (3MG_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-3-methyladenine glycosylase
EC=3.2.2.21

Alternative name(s):
3-alkyladenine DNA glycosylase
3-methyladenine DNA glycosidase
ADPG
N-methylpurine-DNA glycosylase

Gene names

Name:	Mpg
Synonyms:	Mid1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.
Catalytic activity	Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.
Subunit structure	Binds MBD1 By similarity.
Subcellular location	Nucleus Potential.
Sequence similarities	Belongs to the DNA glycosylase MPG family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	base-excision repair Inferred from mutant phenotype. Source: MGI
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-3-methyladenine glycosylase activity Inferred from electronic annotation. Source: EC DNA-3-methylguanine glycosylase activity Inferred from electronic annotation. Source: EC DNA-7-methyladenine glycosylase activity Inferred from electronic annotation. Source: EC DNA-7-methylguanine glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	DNA-3-methyladenine glycosylase		PRO_0000100066
Amino acid modifications
	Modified residue	98	1	Phosphoserine By similarity
	Modified residue	272	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	14	1	A → S in CAA52615. Ref.1
	Sequence conflict	14	1	A → S in AAA19487. Ref.2
	Sequence conflict	14	1	A → S in AAH14754. Ref.5
	Sequence conflict	319 – 324	6	PQQTAC → LANSLL in AAA02577. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q04841 [UniParc]. Last modified July 27, 2011. Version 3. Checksum: 195E96174BFA0576	FASTA	333	36,472
	10 20 30 40 50 60 MPARGGSARP GRGALKPVSV TLLPDTEQPP FLGRARRPGN ARAGSLVTGY HEVGQMPAPL 70 80 90 100 110 120 SRKIGQKKQR LADSEQQQTP KERLLSTPGL RRSIYFSSPE DHSGRLGPEF FDQPAVTLAR 130 140 150 160 170 180 AFLGQVLVRR LADGTELRGR IVETEAYLGP EDEAAHSRGG RQTPRNRGMF MKPGTLYVYL 190 200 210 220 230 240 IYGMYFCLNV SSQGAGACVL LRALEPLEGL ETMRQLRNSL RKSTVGRSLK DRELCSGPSK 250 260 270 280 290 300 LCQALAIDKS FDQRDLAQDD AVWLEHGPLE SSSPAVVVAA ARIGIGHAGE WTQKPLRFYV 310 320 330 QGSPWVSVVD RVAEQMDQPQ QTACSEGLLI VQK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural organization of the mouse DNA repair gene, N-methylpurine-DNA glycosylase." Tatsuka M., Ibeanu G.C., Izumi T., Narayan S., Ramana C.V., Kim N.K., Kang W., Roy G., Mitra S. DNA Cell Biol. 14:37-45(1995) [PubMed: 7832991] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: FEB/N. Tissue: Spleen.
[2]	"Cloning and characterization of a mouse 3-methyladenine/7-methyl-guanine/3-methylguanine DNA glycosylase cDNA whose gene maps to chromosome 11." Engelward B.P., Boosalis M.S., Chen B.J., Deng Z., Siciliano M.J., Samson L.D. Carcinogenesis 14:175-181(1993) [PubMed: 8435858] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Structure of the mouse 3-methyladenine DNA glycosylase gene and exact localization upstream of the alpha-globin gene cluster on chromosome 11." Kielman M.F., Smits R., Bernini L.F. Mamm. Genome 6:499-504(1995) [PubMed: 8589517] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Mammary tumor.
[6]	"Homology of a 130-kb region enclosing the alpha-globin gene cluster, the alpha-locus controlling region, and two non-globin genes in human and mouse." Kielman M., Smits R., Devi T., Fodde R., Bernini L.F. Mamm. Genome 4:314-323(1993) [PubMed: 8318735] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 250-324.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X74509 , X75038, X75039, X75040 Genomic DNA. Translation: CAA52615.1. U10420 mRNA. Translation: AAA19487.1. S81162 Genomic DNA. No translation available. S81120 Genomic DNA. No translation available. S81133 Genomic DNA. No translation available. S81134 Genomic DNA. No translation available. AL929446 Genomic DNA. Translation: CAM16547.1. BC014754 mRNA. Translation: AAH14754.1. M99625 mRNA. Translation: AAA02577.1.
IPI	IPI00110952.
PIR	A49003. I62129.
RefSeq	NP_034952.2. NM_010822.3.
UniGene	Mm.263161.
3D structure databases
ProteinModelPortal	Q04841.
SMR	Q04841. Positions 102-314.
ModBase	Search...
Protein-protein interaction databases
STRING	Q04841.
PTM databases
PhosphoSite	Q04841.
Proteomic databases
PRIDE	Q04841.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000020528; ENSMUSP00000020528; ENSMUSG00000020287.
GeneID	268395.
KEGG	mmu:268395.
Organism-specific databases
CTD	4350.
MGI	MGI:97073. Mpg.
Phylogenomic databases
eggNOG	roNOG09926.
HOGENOM	HBG664239.
HOVERGEN	HBG000019.
InParanoid	Q04841.
OrthoDB	EOG4KD6N3.
Gene expression databases
ArrayExpress	Q04841.
Bgee	Q04841.
CleanEx	MM_MID1. MM_MPG.
Genevestigator	Q04841.
GermOnline	ENSMUSG00000020287. Mus musculus.
Family and domain databases
InterPro	IPR011034. Formyl_transferase_C-like. IPR003180. PurDNA_glycsylse. [Graphical view]
Gene3D	G3DSA:3.10.300.10. PurDNA_glycsylse. 1 hit.
KO	K03652.
PANTHER	PTHR10429. PurDNA_glycsylse. 1 hit.
Pfam	PF02245. Pur_DNA_glyco. 1 hit. [Graphical view]
SUPFAM	SSF50486. FMT_C_like. 1 hit.
TIGRFAMs	TIGR00567. 3mg. 1 hit.
ProtoNet	Search...
Other
NextBio	392271.
SOURCE	Search...

Entry information

Entry name

3MG_MOUSE

Accession

Primary (citable) accession number: Q04841
Secondary accession number(s): A2AVE7, Q64182

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	July 27, 2011
Last modified:	January 25, 2012
This is version 100 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program