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Protein

DNA-3-methyladenine glycosylase

Gene

Mpg

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.

Catalytic activityi

Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.

Enzyme regulationi

Binding to SSBP1 in mitochondria inhibits glycosylase activity in the context of a single-stranded DNA (ssDNA), but not a double-stranded DNA (dsDNA) substrates.By similarity

GO - Molecular functioni

GO - Biological processi

  • base-excision repair Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Enzyme and pathway databases

ReactomeiR-MMU-110331. Cleavage of the damaged purine.
R-MMU-110357. Displacement of DNA glycosylase by APEX1.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-3-methyladenine glycosylase (EC:3.2.2.21)
Alternative name(s):
3-alkyladenine DNA glycosylase
3-methyladenine DNA glycosidase
ADPG
N-methylpurine-DNA glycosylase
Gene namesi
Name:Mpg
Synonyms:Mid1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:97073. Mpg.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Mitochondrion nucleoid, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001000661 – 333DNA-3-methyladenine glycosylaseAdd BLAST333

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei98PhosphoserineBy similarity1
Modified residuei272PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ04841.
MaxQBiQ04841.
PaxDbiQ04841.
PeptideAtlasiQ04841.
PRIDEiQ04841.

PTM databases

iPTMnetiQ04841.
PhosphoSitePlusiQ04841.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020287.
CleanExiMM_MID1.
MM_MPG.
ExpressionAtlasiQ04841. baseline and differential.
GenevisibleiQ04841. MM.

Interactioni

Subunit structurei

Binds MBD1. Binds SSBP1.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020528.

Structurei

3D structure databases

ProteinModelPortaliQ04841.
SMRiQ04841.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA glycosylase MPG family.Curated

Phylogenomic databases

eggNOGiKOG4486. Eukaryota.
COG2094. LUCA.
GeneTreeiENSGT00390000009825.
HOGENOMiHOG000224224.
HOVERGENiHBG000019.
InParanoidiQ04841.
KOiK03652.
OMAiATKPLRF.
OrthoDBiEOG091G0NE4.
TreeFamiTF331768.

Family and domain databases

CDDicd00540. AAG. 1 hit.
Gene3Di3.10.300.10. 1 hit.
HAMAPiMF_00527. 3MGH. 1 hit.
InterProiIPR011034. Formyl_transferase_C-like.
IPR003180. PurDNA_glycsylse.
[Graphical view]
PANTHERiPTHR10429. PTHR10429. 1 hit.
PfamiPF02245. Pur_DNA_glyco. 1 hit.
[Graphical view]
SUPFAMiSSF50486. SSF50486. 1 hit.
TIGRFAMsiTIGR00567. 3mg. 1 hit.

Sequencei

Sequence statusi: Complete.

Q04841-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPARGGSARP GRGALKPVSV TLLPDTEQPP FLGRARRPGN ARAGSLVTGY
60 70 80 90 100
HEVGQMPAPL SRKIGQKKQR LADSEQQQTP KERLLSTPGL RRSIYFSSPE
110 120 130 140 150
DHSGRLGPEF FDQPAVTLAR AFLGQVLVRR LADGTELRGR IVETEAYLGP
160 170 180 190 200
EDEAAHSRGG RQTPRNRGMF MKPGTLYVYL IYGMYFCLNV SSQGAGACVL
210 220 230 240 250
LRALEPLEGL ETMRQLRNSL RKSTVGRSLK DRELCSGPSK LCQALAIDKS
260 270 280 290 300
FDQRDLAQDD AVWLEHGPLE SSSPAVVVAA ARIGIGHAGE WTQKPLRFYV
310 320 330
QGSPWVSVVD RVAEQMDQPQ QTACSEGLLI VQK
Length:333
Mass (Da):36,472
Last modified:July 27, 2011 - v3
Checksum:i195E96174BFA0576
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti14A → S in CAA52615 (PubMed:7832991).Curated1
Sequence conflicti14A → S in AAA19487 (PubMed:8435858).Curated1
Sequence conflicti14A → S in AAH14754 (PubMed:15489334).Curated1
Sequence conflicti319 – 324PQQTAC → LANSLL in AAA02577 (PubMed:8318735).Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74509
, X75038, X75039, X75040 Genomic DNA. Translation: CAA52615.1.
U10420 mRNA. Translation: AAA19487.1.
S81162 Genomic DNA. No translation available.
S81120 Genomic DNA. No translation available.
S81133 Genomic DNA. No translation available.
S81134 Genomic DNA. No translation available.
AL929446 Genomic DNA. Translation: CAM16547.1.
BC014754 mRNA. Translation: AAH14754.1.
M99625 mRNA. Translation: AAA02577.1.
CCDSiCCDS24520.1.
PIRiA49003.
I62129.
RefSeqiNP_034952.2. NM_010822.3.
UniGeneiMm.263161.
Mm.491092.

Genome annotation databases

EnsembliENSMUST00000020528; ENSMUSP00000020528; ENSMUSG00000020287.
GeneIDi268395.
KEGGimmu:268395.
UCSCiuc007ijd.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74509
, X75038, X75039, X75040 Genomic DNA. Translation: CAA52615.1.
U10420 mRNA. Translation: AAA19487.1.
S81162 Genomic DNA. No translation available.
S81120 Genomic DNA. No translation available.
S81133 Genomic DNA. No translation available.
S81134 Genomic DNA. No translation available.
AL929446 Genomic DNA. Translation: CAM16547.1.
BC014754 mRNA. Translation: AAH14754.1.
M99625 mRNA. Translation: AAA02577.1.
CCDSiCCDS24520.1.
PIRiA49003.
I62129.
RefSeqiNP_034952.2. NM_010822.3.
UniGeneiMm.263161.
Mm.491092.

3D structure databases

ProteinModelPortaliQ04841.
SMRiQ04841.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020528.

PTM databases

iPTMnetiQ04841.
PhosphoSitePlusiQ04841.

Proteomic databases

EPDiQ04841.
MaxQBiQ04841.
PaxDbiQ04841.
PeptideAtlasiQ04841.
PRIDEiQ04841.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020528; ENSMUSP00000020528; ENSMUSG00000020287.
GeneIDi268395.
KEGGimmu:268395.
UCSCiuc007ijd.2. mouse.

Organism-specific databases

CTDi4350.
MGIiMGI:97073. Mpg.

Phylogenomic databases

eggNOGiKOG4486. Eukaryota.
COG2094. LUCA.
GeneTreeiENSGT00390000009825.
HOGENOMiHOG000224224.
HOVERGENiHBG000019.
InParanoidiQ04841.
KOiK03652.
OMAiATKPLRF.
OrthoDBiEOG091G0NE4.
TreeFamiTF331768.

Enzyme and pathway databases

ReactomeiR-MMU-110331. Cleavage of the damaged purine.
R-MMU-110357. Displacement of DNA glycosylase by APEX1.

Miscellaneous databases

PROiQ04841.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020287.
CleanExiMM_MID1.
MM_MPG.
ExpressionAtlasiQ04841. baseline and differential.
GenevisibleiQ04841. MM.

Family and domain databases

CDDicd00540. AAG. 1 hit.
Gene3Di3.10.300.10. 1 hit.
HAMAPiMF_00527. 3MGH. 1 hit.
InterProiIPR011034. Formyl_transferase_C-like.
IPR003180. PurDNA_glycsylse.
[Graphical view]
PANTHERiPTHR10429. PTHR10429. 1 hit.
PfamiPF02245. Pur_DNA_glyco. 1 hit.
[Graphical view]
SUPFAMiSSF50486. SSF50486. 1 hit.
TIGRFAMsiTIGR00567. 3mg. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry namei3MG_MOUSE
AccessioniPrimary (citable) accession number: Q04841
Secondary accession number(s): A2AVE7, Q64182
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.