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Protein

mRNA transport factor GFD1

Gene

GFD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

High-copy suppressor of mutant alleles of ATP-dependent RNA helicase DBP5, which is involved in mRNA export from the nucleus. It may also play an important role in a late stage of NAB2-mRNA export.3 Publications

GO - Biological processi

  • mRNA export from nucleus Source: SGD
  • protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32931-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
mRNA transport factor GFD1
Alternative name(s):
Good for full DBP5 activity protein 1
Gene namesi
Name:GFD1
Ordered Locus Names:YMR255W
ORF Names:YM9920.09
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR255W.
SGDiS000004868. GFD1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nuclear membrane Source: UniProtKB-SubCell
  • nuclear pore Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 188188mRNA transport factor GFD1PRO_0000087477Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei87 – 871PhosphoserineCombined sources
Modified residuei106 – 1061PhosphoserineCombined sources
Modified residuei111 – 1111PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ04839.

PTM databases

iPTMnetiQ04839.

Interactioni

Subunit structurei

Interacts with GLE1, NUP42, NAB2, ZDS1 and probably DBP5. Forms a complex with GLE1 and NAB2.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DBP5P204492EBI-27549,EBI-5617
NAB2P325057EBI-27549,EBI-11770
ZDS1P501113EBI-27549,EBI-29626

Protein-protein interaction databases

BioGridi35433. 37 interactions.
DIPiDIP-1254N.
IntActiQ04839. 9 interactions.
MINTiMINT-395325.

Structurei

Secondary structure

1
188
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi127 – 14923Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3LCNX-ray2.00C/D123-151[»]
ProteinModelPortaliQ04839.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ04839.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili119 – 16446Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi17 – 136120Lys-richAdd
BLAST
Compositional biasi152 – 1554Poly-Gln

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOGENOMiHOG000066053.
InParanoidiQ04839.
KOiK18731.
OrthoDBiEOG7N37RP.

Family and domain databases

InterProiIPR020401. mRNA_transport_factor_GFD1.
[Graphical view]
ProDomiPD098974. mRNA_transport_factor_GFD1. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Q04839-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLESIWADA PDEEPIKKQK PSHKRSNNNK KNNNSRWSNE SSSNNKKKDS
60 70 80 90 100
VNKVKNNKGN HESKTKNKIK ETLPREKKPP HSQGKISPVS ESLAINPFSQ
110 120 130 140 150
KATEISPPPV SPSKMKTTKT QSKQDTASKM KLLKKKIEEQ REILQKTHHK
160 170 180
NQQQQVLMDF LNDEGSSNWV DDDEEELILQ RLKTSLKI
Length:188
Mass (Da):21,571
Last modified:November 1, 1997 - v1
Checksum:i6C835BD4BD048EB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF007064 Genomic DNA. Translation: AAC50003.1.
Z48639 Genomic DNA. Translation: CAA88582.1.
BK006946 Genomic DNA. Translation: DAA10155.1.
PIRiS53077.
RefSeqiNP_013982.1. NM_001182762.1.

Genome annotation databases

EnsemblFungiiYMR255W; YMR255W; YMR255W.
GeneIDi855297.
KEGGisce:YMR255W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF007064 Genomic DNA. Translation: AAC50003.1.
Z48639 Genomic DNA. Translation: CAA88582.1.
BK006946 Genomic DNA. Translation: DAA10155.1.
PIRiS53077.
RefSeqiNP_013982.1. NM_001182762.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3LCNX-ray2.00C/D123-151[»]
ProteinModelPortaliQ04839.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35433. 37 interactions.
DIPiDIP-1254N.
IntActiQ04839. 9 interactions.
MINTiMINT-395325.

PTM databases

iPTMnetiQ04839.

Proteomic databases

MaxQBiQ04839.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR255W; YMR255W; YMR255W.
GeneIDi855297.
KEGGisce:YMR255W.

Organism-specific databases

EuPathDBiFungiDB:YMR255W.
SGDiS000004868. GFD1.

Phylogenomic databases

HOGENOMiHOG000066053.
InParanoidiQ04839.
KOiK18731.
OrthoDBiEOG7N37RP.

Enzyme and pathway databases

BioCyciYEAST:G3O-32931-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ04839.
PROiQ04839.

Family and domain databases

InterProiIPR020401. mRNA_transport_factor_GFD1.
[Graphical view]
ProDomiPD098974. mRNA_transport_factor_GFD1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Study of a region on yeast chromosome XIII that complements pet G199 mutants (COX7) and carries a new non-essential gene."
    Nobrega M.P., Graminha M.A., Troitskaya E.N., Nobrega F.G.
    Braz. J. Med. Biol. Res. 31:355-363(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The RNA export factor Gle1p is located on the cytoplasmic fibrils of the NPC and physically interacts with the FG-nucleoporin Rip1p, the DEAD-box protein Rat8p/Dbp5p and a new protein Ymr255p."
    Strahm Y., Fahrenkrog B., Zenklusen D., Rychner E., Kantor J., Rosbach M., Stutz F.
    EMBO J. 18:5761-5777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH GLE1 AND NUP42.
  5. "Rat8p/Dbp5p is a shuttling transport factor that interacts with Rat7p/Nup159p and Gle1p and suppresses the mRNA export defect of xpo1-1 cells."
    Hodge C.A., Colot H.V., Stafford P., Cole C.N.
    EMBO J. 18:5778-5788(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH DBP5, SUBCELLULAR LOCATION.
  6. "Nuclear export of the yeast mRNA-binding protein Nab2 is linked to a direct interaction with Gfd1 and to Gle1 function."
    Suntharalingam M., Alcazar-Roman A.R., Wente S.R.
    J. Biol. Chem. 279:35384-35391(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH NAB2.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Physical and genetic interactions link the yeast protein Zds1p with mRNA nuclear export."
    Estruch F., Hodge C.A., Rodriguez-Navarro S., Cole C.N.
    J. Biol. Chem. 280:9691-9697(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ZDS1.
  9. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87; SER-106 AND SER-111, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87 AND SER-106, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGFD1_YEAST
AccessioniPrimary (citable) accession number: Q04839
Secondary accession number(s): D6W081
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 8, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2733 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.