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Q04837

- SSBP_HUMAN

UniProt

Q04837 - SSBP_HUMAN

Protein

Single-stranded DNA-binding protein, mitochondrial

Gene

SSBP1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    This protein binds preferentially and cooperatively to ss-DNA. Probably involved in mitochondrial DNA replication. Associates with mitochondrial DNA.

    GO - Molecular functioni

    1. chromatin binding Source: UniProtKB
    2. poly(A) RNA binding Source: UniProtKB
    3. protein binding Source: IntAct
    4. single-stranded DNA binding Source: InterPro

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW
    2. mitochondrion morphogenesis Source: Ensembl
    3. positive regulation of helicase activity Source: UniProtKB

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_200608. Transcriptional activation of mitochondrial biogenesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Single-stranded DNA-binding protein, mitochondrial
    Short name:
    Mt-SSB
    Short name:
    MtSSB
    Alternative name(s):
    PWP1-interacting protein 17
    Gene namesi
    Name:SSBP1
    Synonyms:SSBP
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 7

    Organism-specific databases

    HGNCiHGNC:11317. SSBP1.

    Subcellular locationi

    Mitochondrion 1 Publication. Mitochondrion matrixmitochondrion nucleoid 1 Publication

    GO - Cellular componenti

    1. extracellular vesicular exosome Source: UniProt
    2. mitochondrial nucleoid Source: BHF-UCL
    3. mitochondrion Source: HPA
    4. nucleus Source: UniProt

    Keywords - Cellular componenti

    Mitochondrion, Mitochondrion nucleoid

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA36141.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 1616Mitochondrion1 PublicationAdd
    BLAST
    Chaini17 – 148132Single-stranded DNA-binding protein, mitochondrialPRO_0000033263Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei79 – 791Phosphoserine1 Publication
    Modified residuei113 – 1131N6-acetyllysine1 Publication
    Modified residuei122 – 1221N6-succinyllysineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ04837.
    PaxDbiQ04837.
    PeptideAtlasiQ04837.
    PRIDEiQ04837.

    2D gel databases

    UCD-2DPAGEQ04837.

    PTM databases

    PhosphoSiteiQ04837.

    Expressioni

    Gene expression databases

    ArrayExpressiQ04837.
    BgeeiQ04837.
    CleanExiHS_SSBP1.
    GenevestigatoriQ04837.

    Organism-specific databases

    HPAiHPA002866.

    Interactioni

    Subunit structurei

    Homotetramer By similarity. Interacts with MPG/AAG, through inhibition of its glycosylase activity it potentially prevents formation of DNA breaks in ssDNA, ensuring that base removal primarily occurs in dsDNA.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    revP046183EBI-353460,EBI-6164309From a different organism.

    Protein-protein interaction databases

    BioGridi112620. 80 interactions.
    IntActiQ04837. 27 interactions.
    MINTiMINT-3025472.
    STRINGi9606.ENSP00000265304.

    Structurei

    Secondary structure

    1
    148
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi30 – 3910
    Beta strandi44 – 463
    Beta strandi48 – 514
    Beta strandi54 – 6512
    Beta strandi79 – 8911
    Helixi94 – 1018
    Beta strandi107 – 1159
    Beta strandi119 – 1268
    Beta strandi130 – 1389

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1S3OX-ray2.47A/B17-148[»]
    2DUDX-ray2.70A/B16-148[»]
    3ULLX-ray2.40A/B17-148[»]
    ProteinModelPortaliQ04837.
    SMRiQ04837. Positions 26-140.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ04837.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini30 – 141112SSBAdd
    BLAST

    Sequence similaritiesi

    Contains 1 SSB domain.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0629.
    HOVERGENiHBG001181.
    InParanoidiQ04837.
    KOiK03111.
    OMAiRNPVTIF.
    PhylomeDBiQ04837.
    TreeFamiTF314629.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    HAMAPiMF_00984. SSB.
    InterProiIPR012340. NA-bd_OB-fold.
    IPR000424. Primosome_PriB/ssb.
    IPR011344. ssDNA-bd.
    [Graphical view]
    PANTHERiPTHR10302. PTHR10302. 1 hit.
    PfamiPF00436. SSB. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002070. SSB. 1 hit.
    SUPFAMiSSF50249. SSF50249. 1 hit.
    TIGRFAMsiTIGR00621. ssb. 1 hit.
    PROSITEiPS50935. SSB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q04837-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFRRPVLQVL RQFVRHESET TTSLVLERSL NRVHLLGRVG QDPVLRQVEG    50
    KNPVTIFSLA TNEMWRSGDS EVYQLGDVSQ KTTWHRISVF RPGLRDVAYQ 100
    YVKKGSRIYL EGKIDYGEYM DKNNVRRQAT TIIADNIIFL SDQTKEKE 148
    Length:148
    Mass (Da):17,260
    Last modified:October 1, 1993 - v1
    Checksum:i98EE9E396D5636C2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M94556 mRNA. Translation: AAA36332.1.
    AF277319 mRNA. Translation: AAK69112.1.
    BC000895 mRNA. Translation: AAH00895.1.
    CCDSiCCDS5866.1.
    PIRiJN0568.
    RefSeqiNP_001243439.1. NM_001256510.1.
    NP_001243440.1. NM_001256511.1.
    NP_001243441.1. NM_001256512.1.
    NP_001243442.1. NM_001256513.1.
    NP_003134.1. NM_003143.2.
    UniGeneiHs.490394.

    Genome annotation databases

    EnsembliENST00000265304; ENSP00000265304; ENSG00000106028.
    ENST00000465582; ENSP00000420485; ENSG00000106028.
    ENST00000481508; ENSP00000419665; ENSG00000106028.
    ENST00000484178; ENSP00000419388; ENSG00000106028.
    ENST00000498107; ENSP00000419541; ENSG00000106028.
    ENST00000570667; ENSP00000460028; ENSG00000262771.
    ENST00000571430; ENSP00000459367; ENSG00000262771.
    ENST00000574199; ENSP00000461884; ENSG00000262771.
    ENST00000575059; ENSP00000458815; ENSG00000262771.
    ENST00000576834; ENSP00000459208; ENSG00000262771.
    GeneIDi6742.
    KEGGihsa:6742.
    UCSCiuc003vwo.2. human.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M94556 mRNA. Translation: AAA36332.1 .
    AF277319 mRNA. Translation: AAK69112.1 .
    BC000895 mRNA. Translation: AAH00895.1 .
    CCDSi CCDS5866.1.
    PIRi JN0568.
    RefSeqi NP_001243439.1. NM_001256510.1.
    NP_001243440.1. NM_001256511.1.
    NP_001243441.1. NM_001256512.1.
    NP_001243442.1. NM_001256513.1.
    NP_003134.1. NM_003143.2.
    UniGenei Hs.490394.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1S3O X-ray 2.47 A/B 17-148 [» ]
    2DUD X-ray 2.70 A/B 16-148 [» ]
    3ULL X-ray 2.40 A/B 17-148 [» ]
    ProteinModelPortali Q04837.
    SMRi Q04837. Positions 26-140.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112620. 80 interactions.
    IntActi Q04837. 27 interactions.
    MINTi MINT-3025472.
    STRINGi 9606.ENSP00000265304.

    PTM databases

    PhosphoSitei Q04837.

    2D gel databases

    UCD-2DPAGE Q04837.

    Proteomic databases

    MaxQBi Q04837.
    PaxDbi Q04837.
    PeptideAtlasi Q04837.
    PRIDEi Q04837.

    Protocols and materials databases

    DNASUi 6742.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000265304 ; ENSP00000265304 ; ENSG00000106028 .
    ENST00000465582 ; ENSP00000420485 ; ENSG00000106028 .
    ENST00000481508 ; ENSP00000419665 ; ENSG00000106028 .
    ENST00000484178 ; ENSP00000419388 ; ENSG00000106028 .
    ENST00000498107 ; ENSP00000419541 ; ENSG00000106028 .
    ENST00000570667 ; ENSP00000460028 ; ENSG00000262771 .
    ENST00000571430 ; ENSP00000459367 ; ENSG00000262771 .
    ENST00000574199 ; ENSP00000461884 ; ENSG00000262771 .
    ENST00000575059 ; ENSP00000458815 ; ENSG00000262771 .
    ENST00000576834 ; ENSP00000459208 ; ENSG00000262771 .
    GeneIDi 6742.
    KEGGi hsa:6742.
    UCSCi uc003vwo.2. human.

    Organism-specific databases

    CTDi 6742.
    GeneCardsi GC07P141442.
    HGNCi HGNC:11317. SSBP1.
    HPAi HPA002866.
    MIMi 600439. gene.
    neXtProti NX_Q04837.
    PharmGKBi PA36141.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0629.
    HOVERGENi HBG001181.
    InParanoidi Q04837.
    KOi K03111.
    OMAi RNPVTIF.
    PhylomeDBi Q04837.
    TreeFami TF314629.

    Enzyme and pathway databases

    Reactomei REACT_200608. Transcriptional activation of mitochondrial biogenesis.

    Miscellaneous databases

    ChiTaRSi SSBP1. human.
    EvolutionaryTracei Q04837.
    GeneWikii SSBP1.
    GenomeRNAii 6742.
    NextBioi 26300.
    PROi Q04837.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q04837.
    Bgeei Q04837.
    CleanExi HS_SSBP1.
    Genevestigatori Q04837.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    HAMAPi MF_00984. SSB.
    InterProi IPR012340. NA-bd_OB-fold.
    IPR000424. Primosome_PriB/ssb.
    IPR011344. ssDNA-bd.
    [Graphical view ]
    PANTHERi PTHR10302. PTHR10302. 1 hit.
    Pfami PF00436. SSB. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002070. SSB. 1 hit.
    SUPFAMi SSF50249. SSF50249. 1 hit.
    TIGRFAMsi TIGR00621. ssb. 1 hit.
    PROSITEi PS50935. SSB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of human and rat cDNAs encoding the mitochondrial single-stranded DNA-binding protein (SSB)."
      Tiranti V., Rocchi M., Didonato S., Zeviani M.
      Gene 126:219-225(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "hPWP1-interacting protein 17 (ssDNA BP)."
      Honore B.
      Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Cervix.
    4. "Global profiling of protease cleavage sites by chemoselective labeling of protein N-termini."
      Xu G., Shin S.B., Jaffrey S.R.
      Proc. Natl. Acad. Sci. U.S.A. 106:19310-19315(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 17-29.
      Tissue: Leukemic T-cell.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic kidney.
    6. "The layered structure of human mitochondrial DNA nucleoids."
      Bogenhagen D.F., Rousseau D., Burke S.
      J. Biol. Chem. 283:3665-3675(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, ASSOCIATION WITH MITOCHONDRIAL DNA, IDENTIFICATION BY MASS SPECTROMETRY.
    7. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
      Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
      Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-113, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Alkyladenine DNA glycosylase (AAG) localizes to mitochondria and interacts with mitochondrial single-stranded binding protein (mtSSB)."
      van Loon B., Samson L.D.
      DNA Repair 12:177-187(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, INTERACTION WITH MPG.
    10. "Crystal structure of human mitochondrial single-stranded DNA binding protein at 2.4-A resolution."
      Yang C., Curth U., Urbanke C., Kang C.
      Nat. Struct. Biol. 4:153-157(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 26-141.
    11. "Crystal structure of human mitochondrial single-stranded DNA-binding protein (HMTSSB)."
      RIKEN structural genomics initiative (RSGI)
      Submitted (JAN-2007) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 16-148.

    Entry informationi

    Entry nameiSSBP_HUMAN
    AccessioniPrimary (citable) accession number: Q04837
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 147 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 7
      Human chromosome 7: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3