Single-stranded DNA-binding protein, mitochondrial precursor

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Q04837 (SSBP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 122. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Single-stranded DNA-binding protein, mitochondrial
Short name=Mt-SSB
Short name=MtSSB

Alternative name(s):
PWP1-interacting protein 17

Gene names

Name:	SSBP1
Synonyms:	SSBP

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	148 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein binds preferentially and cooperatively to ss-DNA. Probably involved in mitochondrial DNA replication. Associates with mitochondrial DNA.
Subunit structure	Homotetramer By similarity.
Subcellular location	Mitochondrion. Mitochondrion matrix › mitochondrion nucleoid Ref.6.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.5
Sequence similarities	Contains 1 SSB domain.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Mitochondrion Mitochondrion nucleoid
Domain	Transit peptide
Ligand	DNA-binding
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of helicase activity Inferred from direct assay. Source: UniProtKB
Cellular component	mitochondrial nucleoid Inferred from direct assay Ref.6. Source: BHF-UCL
Molecular function	chromatin binding Inferred from direct assay Ref.6. Source: UniProtKB single-stranded DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 16

Mitochondrion Ref.4

Chain

17 – 148

132

Single-stranded DNA-binding protein, mitochondrial

PRO_0000033263

Regions

Domain

30 – 141

112

SSB

Amino acid modifications

Modified residue

Phosphoserine Ref.5

Modified residue

113

N6-acetyllysine Ref.7

Secondary structure

148

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q04837 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 98EE9E396D5636C2
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FASTA

148

17,260

        10         20         30         40         50         60 
MFRRPVLQVL RQFVRHESET TTSLVLERSL NRVHLLGRVG QDPVLRQVEG KNPVTIFSLA 

        70         80         90        100        110        120 
TNEMWRSGDS EVYQLGDVSQ KTTWHRISVF RPGLRDVAYQ YVKKGSRIYL EGKIDYGEYM 

       130        140 
DKNNVRRQAT TIIADNIIFL SDQTKEKE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of human and rat cDNAs encoding the mitochondrial single-stranded DNA-binding protein (SSB)." Tiranti V., Rocchi M., Didonato S., Zeviani M. Gene 126:219-225(1993) [PubMed: 8482537] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"hPWP1-interacting protein 17 (ssDNA BP)." Honore B. Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cervix.
[4]	"Global profiling of protease cleavage sites by chemoselective labeling of protein N-termini." Xu G., Shin S.B., Jaffrey S.R. Proc. Natl. Acad. Sci. U.S.A. 106:19310-19315(2009) [PubMed: 19892738] [Abstract] Cited for: PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 17-29. Tissue: Leukemic T-cell.
[5]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[6]	"The layered structure of human mitochondrial DNA nucleoids." Bogenhagen D.F., Rousseau D., Burke S. J. Biol. Chem. 283:3665-3675(2008) [PubMed: 18063578] [Abstract] Cited for: SUBCELLULAR LOCATION, ASSOCIATION WITH MITOCHONDRIAL DNA, IDENTIFICATION BY MASS SPECTROMETRY.
[7]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-113, MASS SPECTROMETRY.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]	"Crystal structure of human mitochondrial single-stranded DNA binding protein at 2.4-A resolution." Yang C., Curth U., Urbanke C., Kang C. Nat. Struct. Biol. 4:153-157(1997) [PubMed: 9033597] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 26-141.
[10]	"Crystal structure of human mitochondrial single-stranded DNA-binding protein (HMTSSB)." RIKEN structural genomics initiative (RSGI) Submitted (JAN-2007) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 16-148.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M94556 mRNA. Translation: AAA36332.1.
AF277319 mRNA. Translation: AAK69112.1.
BC000895 mRNA. Translation: AAH00895.1.

IPI

IPI00029744.

PIR

JN0568.

RefSeq

NP_003134.1. NM_003143.1.

UniGene

Hs.490394.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1S3O	X-ray	2.47	A/B	17-148	[»]
2DUD	X-ray	2.70	A/B	16-148	[»]
3ULL	X-ray	2.40	A/B	17-148	[»]

ProteinModelPortal

Q04837.

SMR

Q04837. Positions 26-140.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q04837. 18 interactions.

MINT

MINT-3025472.

STRING

Q04837.

PTM databases

PhosphoSite

Q04837.

Polymorphism databases

DMDM

417811.

2D gel databases

UCD-2DPAGE

Q04837.

Proteomic databases

PeptideAtlas

Q04837.

PRIDE

Q04837.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000265304; ENSP00000265304; ENSG00000106028.
ENST00000465582; ENSP00000420485; ENSG00000106028.
ENST00000481508; ENSP00000419665; ENSG00000106028.
ENST00000484178; ENSP00000419388; ENSG00000106028.
ENST00000498107; ENSP00000419541; ENSG00000106028.

GeneID

6742.

KEGG

hsa:6742.

UCSC

uc003vwo.1. human.

Organism-specific databases

CTD

6742.

GeneCards

GC07P141440.

H-InvDB

HIX0007155.

HGNC

HGNC:11317. SSBP1.

HPA

HPA002866.

MIM

600439. gene.

neXtProt

NX_Q04837.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

HBG001181.

InParanoid

Q04837.

OMA

RNPVTIF.

OrthoDB

EOG4KSPM4.

PhylomeDB

Q04837.

Gene expression databases

ArrayExpress

Q04837.

Bgee

Q04837.

CleanEx

HS_SSBP1.

Genevestigator

Q04837.

GermOnline

ENSG00000106028. Homo sapiens.

Family and domain databases

InterPro

IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR000424. Primosome_PriB/ssb.
IPR011344. ssDNA-bd.
[Graphical view]

Gene3D

G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.

K03111.

PANTHER

PTHR10302. Single_strand_bd. 1 hit.

Pfam

PF00436. SSB. 1 hit.
[Graphical view]

PIRSF

PIRSF002070. SSB. 1 hit.

SUPFAM

SSF50249. Nucleic_acid_OB. 1 hit.

TIGRFAMs

TIGR00621. Ssb. 1 hit.

PROSITE

PS50935. SSB. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

26300.

SOURCE

Search...

Entry information

Entry name

SSBP_HUMAN

Accession

Primary (citable) accession number: Q04837

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	January 25, 2012
This is version 122 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents