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Protein

31 kDa ribonucleoprotein, chloroplastic

Gene

CP31A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for specific RNA editing events in chloroplasts and stabilizes specific chloroplast mRNAs. Associates with the 3'-terminus ndhF mRNAs and protects them against 3'-exonucleolytic degradation (PubMed:19297624, PubMed:23110894). Required for normal chloroplast development under cold stress conditions by stabilizing transcripts of numerous mRNAs under these conditions (PubMed:23110894). May modulate telomere replication through RNA binding domains (PubMed:14703514).3 Publications

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • poly(U) RNA binding Source: TAIR
  • RNA binding Source: TAIR

GO - Biological processi

  • base conversion or substitution editing Source: TAIR
  • cold acclimation Source: TAIR
  • innate immune response Source: TAIR
  • mRNA processing Source: UniProtKB-KW
  • response to cold Source: TAIR
  • RNA modification Source: TAIR
  • RNA processing Source: TAIR
  • RNA stabilization Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
31 kDa ribonucleoprotein, chloroplastic
Alternative name(s):
RNA-binding protein 1/2/31 Publication
Short name:
AtRBP33
RNA-binding protein CP31A1 Publication
RNA-binding protein RNP-T1 Publication
RNA-binding protein cp311 Publication
Gene namesi
Name:CP31A1 Publication
Synonyms:RBP311 Publication, RNPT1 Publication
Ordered Locus Names:At4g24770
ORF Names:F22K18.30
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G24770.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast stroma Source: TAIR
  • chloroplast thylakoid membrane Source: TAIR
  • intracellular ribonucleoprotein complex Source: UniProtKB-KW
  • thylakoid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but mutant plants exhibit multiple specific editing defects in chloroplast transcripts (PubMed:19297624, PubMed:23110894). Mutant plants have increased sensitivity to cold stress (PubMed:23110894).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 77ChloroplastSequence analysisAdd BLAST77
ChainiPRO_000003102178 – 32931 kDa ribonucleoprotein, chloroplasticAdd BLAST252

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei123PhosphoserineCombined sources1
Modified residuei128PhosphoserineCombined sources1

Post-translational modificationi

ADP-ribosylated by the Pseudomonas syringae type III effector HopU1. ADP-ribosylation reduces the ability of the protein to bind RNA.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ04836.
PRIDEiQ04836.

PTM databases

iPTMnetiQ04836.

Expressioni

Gene expression databases

GenevisibleiQ04836. AT.

Interactioni

Protein-protein interaction databases

BioGridi13868. 2 interactors.
STRINGi3702.AT4G24770.1.

Structurei

3D structure databases

ProteinModelPortaliQ04836.
SMRiQ04836.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati108 – 11215
Repeati113 – 11725
Repeati118 – 12235
Repeati123 – 12745
Repeati128 – 13255
Repeati133 – 13765
Domaini152 – 228RRM 1PROSITE-ProRule annotationAdd BLAST77
Domaini244 – 322RRM 2PROSITE-ProRule annotationAdd BLAST79

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni108 – 1376 X 5 AA approximate tandem repeats of S-E-G-D-XAdd BLAST30

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi86 – 149Asp/Glu-rich (acidic)Add BLAST64

Sequence similaritiesi

Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiENOG410IQWU. Eukaryota.
ENOG4111FDY. LUCA.
HOGENOMiHOG000276232.
InParanoidiQ04836.
OMAiTMSSETE.
OrthoDBiEOG09360O4L.
PhylomeDBiQ04836.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 1 (identifier: Q04836-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSIVTSSL KPLAMADSSS STIFSHPSIS STISSSRIRS SSVSLLTGRI
60 70 80 90 100
NLPLSFSRVS LSLKTKTHLK KSPFVSFVAQ TSDWAEEGGE GSVAVEETEN
110 120 130 140 150
SLESQDVSEG DESEGDASEG DVSEGDESEG DVSEGAVSER AEFPEPSEEA
160 170 180 190 200
KLFVGNLAYD VNSQALAMLF EQAGTVEIAE VIYNRETDQS RGFGFVTMSS
210 220 230 240 250
VDEAETAVEK FNRYDLNGRL LTVNKAAPRG SRPERAPRVY EPAFRVYVGN
260 270 280 290 300
LPWDVDNGRL EQLFSEHGKV VEARVVYDRE TGRSRGFGFV TMSDVDELNE
310 320
AISALDGQNL EGRAIRVNVA EERPPRRGY
Length:329
Mass (Da):35,787
Last modified:February 1, 1994 - v1
Checksum:i4FD02DA48600A593
GO
Isoform 2 (identifier: Q04836-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-14: Missing.

Note: No experimental confirmation available.
Show »
Length:315
Mass (Da):34,401
Checksum:iD2F7E59ACB32FE0D
GO
Isoform 3 (identifier: Q04836-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-167: Missing.

Note: No experimental confirmation available.
Show »
Length:162
Mass (Da):18,318
Checksum:i0667BB1D2CBCCACD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti109 – 123Missing in BAA06520 (PubMed:7894017).CuratedAdd BLAST15
Sequence conflicti109 – 123Missing in BAA06521 (PubMed:7894017).CuratedAdd BLAST15
Sequence conflicti327R → H in AAK95304 (PubMed:14593172).Curated1
Sequence conflicti327R → H in AAN28804 (PubMed:14593172).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0188801 – 167Missing in isoform 3. CuratedAdd BLAST167
Alternative sequenceiVSP_0188791 – 14Missing in isoform 2. CuratedAdd BLAST14

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65255 mRNA. Translation: CAA46347.1.
M94554 mRNA. Translation: AAA32860.1.
U08467 Unassigned RNA. Translation: AAA18378.1.
U08467 Unassigned RNA. Translation: AAA18379.1.
U08467 Unassigned RNA. Translation: AAA18380.1.
D31712 Genomic DNA. Translation: BAA06520.1.
D31713 mRNA. Translation: BAA06521.1.
AL035356 Genomic DNA. Translation: CAA22986.1.
AL161562 Genomic DNA. Translation: CAB79387.1.
CP002687 Genomic DNA. Translation: AEE84954.1.
AF410318 mRNA. Translation: AAK95304.1.
AY143865 mRNA. Translation: AAN28804.1.
PIRiS28057.
S53492.
RefSeqiNP_194208.1. NM_118610.4. [Q04836-1]
UniGeneiAt.38112.
At.70990.

Genome annotation databases

EnsemblPlantsiAT4G24770.1; AT4G24770.1; AT4G24770. [Q04836-1]
GeneIDi828579.
GrameneiAT4G24770.1; AT4G24770.1; AT4G24770.
KEGGiath:AT4G24770.

Keywords - Coding sequence diversityi

Alternative initiation, RNA editing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65255 mRNA. Translation: CAA46347.1.
M94554 mRNA. Translation: AAA32860.1.
U08467 Unassigned RNA. Translation: AAA18378.1.
U08467 Unassigned RNA. Translation: AAA18379.1.
U08467 Unassigned RNA. Translation: AAA18380.1.
D31712 Genomic DNA. Translation: BAA06520.1.
D31713 mRNA. Translation: BAA06521.1.
AL035356 Genomic DNA. Translation: CAA22986.1.
AL161562 Genomic DNA. Translation: CAB79387.1.
CP002687 Genomic DNA. Translation: AEE84954.1.
AF410318 mRNA. Translation: AAK95304.1.
AY143865 mRNA. Translation: AAN28804.1.
PIRiS28057.
S53492.
RefSeqiNP_194208.1. NM_118610.4. [Q04836-1]
UniGeneiAt.38112.
At.70990.

3D structure databases

ProteinModelPortaliQ04836.
SMRiQ04836.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi13868. 2 interactors.
STRINGi3702.AT4G24770.1.

PTM databases

iPTMnetiQ04836.

Proteomic databases

PaxDbiQ04836.
PRIDEiQ04836.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G24770.1; AT4G24770.1; AT4G24770. [Q04836-1]
GeneIDi828579.
GrameneiAT4G24770.1; AT4G24770.1; AT4G24770.
KEGGiath:AT4G24770.

Organism-specific databases

TAIRiAT4G24770.

Phylogenomic databases

eggNOGiENOG410IQWU. Eukaryota.
ENOG4111FDY. LUCA.
HOGENOMiHOG000276232.
InParanoidiQ04836.
OMAiTMSSETE.
OrthoDBiEOG09360O4L.
PhylomeDBiQ04836.

Miscellaneous databases

PROiQ04836.

Gene expression databases

GenevisibleiQ04836. AT.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCP31A_ARATH
AccessioniPrimary (citable) accession number: Q04836
Secondary accession number(s): P92871
, Q03394, Q43350, Q94EH5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 30, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.