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Protein

31 kDa ribonucleoprotein, chloroplastic

Gene

CP31A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for specific RNA editing events in chloroplasts and stabilizes specific chloroplast mRNAs. Associates with the 3'-terminus ndhF mRNAs and protects them against 3'-exonucleolytic degradation (PubMed:19297624, PubMed:23110894). Required for normal chloroplast development under cold stress conditions by stabilizing transcripts of numerous mRNAs under these conditions (PubMed:23110894). May modulate telomere replication through RNA binding domains (PubMed:14703514).3 Publications

GO - Molecular functioni

  • mRNA binding Source: TAIR
  • poly(U) RNA binding Source: TAIR
  • RNA binding Source: TAIR

GO - Biological processi

  • base conversion or substitution editing Source: TAIR
  • cold acclimation Source: TAIR
  • innate immune response Source: TAIR
  • mRNA processing Source: UniProtKB-KW
  • response to cold Source: TAIR
  • RNA modification Source: TAIR
  • RNA processing Source: TAIR
  • RNA stabilization Source: TAIR

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
Biological processmRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
31 kDa ribonucleoprotein, chloroplastic
Alternative name(s):
RNA-binding protein 1/2/31 Publication
Short name:
AtRBP33
RNA-binding protein CP31A1 Publication
RNA-binding protein RNP-T1 Publication
RNA-binding protein cp311 Publication
Gene namesi
Name:CP31A1 Publication
Synonyms:RBP311 Publication, RNPT1 Publication
Ordered Locus Names:At4g24770
ORF Names:F22K18.30
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G24770
TAIRilocus:2122009 AT4G24770

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but mutant plants exhibit multiple specific editing defects in chloroplast transcripts (PubMed:19297624, PubMed:23110894). Mutant plants have increased sensitivity to cold stress (PubMed:23110894).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 77ChloroplastSequence analysisAdd BLAST77
ChainiPRO_000003102178 – 32931 kDa ribonucleoprotein, chloroplasticAdd BLAST252

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei123PhosphoserineCombined sources1
Modified residuei128PhosphoserineCombined sources1

Post-translational modificationi

ADP-ribosylated by the Pseudomonas syringae type III effector HopU1. ADP-ribosylation reduces the ability of the protein to bind RNA.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ04836
PRIDEiQ04836

PTM databases

iPTMnetiQ04836

Expressioni

Gene expression databases

ExpressionAtlasiQ04836 baseline and differential
GenevisibleiQ04836 AT

Interactioni

Protein-protein interaction databases

BioGridi13868, 2 interactors
STRINGi3702.AT4G24770.1

Structurei

3D structure databases

ProteinModelPortaliQ04836
SMRiQ04836
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati108 – 11215
Repeati113 – 11725
Repeati118 – 12235
Repeati123 – 12745
Repeati128 – 13255
Repeati133 – 13765
Domaini152 – 228RRM 1PROSITE-ProRule annotationAdd BLAST77
Domaini244 – 322RRM 2PROSITE-ProRule annotationAdd BLAST79

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni108 – 1376 X 5 AA approximate tandem repeats of S-E-G-D-XAdd BLAST30

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi86 – 149Asp/Glu-rich (acidic)Add BLAST64

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiENOG410IQWU Eukaryota
ENOG4111FDY LUCA
HOGENOMiHOG000276232
InParanoidiQ04836
OMAiIFSHPSF
OrthoDBiEOG09360O4L
PhylomeDBiQ04836

Family and domain databases

Gene3Di3.30.70.330, 2 hits
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF00076 RRM_1, 2 hits
SMARTiView protein in SMART
SM00360 RRM, 2 hits
SUPFAMiSSF54928 SSF54928, 2 hits
PROSITEiView protein in PROSITE
PS50102 RRM, 2 hits

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 1 (identifier: Q04836-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSIVTSSL KPLAMADSSS STIFSHPSIS STISSSRIRS SSVSLLTGRI
60 70 80 90 100
NLPLSFSRVS LSLKTKTHLK KSPFVSFVAQ TSDWAEEGGE GSVAVEETEN
110 120 130 140 150
SLESQDVSEG DESEGDASEG DVSEGDESEG DVSEGAVSER AEFPEPSEEA
160 170 180 190 200
KLFVGNLAYD VNSQALAMLF EQAGTVEIAE VIYNRETDQS RGFGFVTMSS
210 220 230 240 250
VDEAETAVEK FNRYDLNGRL LTVNKAAPRG SRPERAPRVY EPAFRVYVGN
260 270 280 290 300
LPWDVDNGRL EQLFSEHGKV VEARVVYDRE TGRSRGFGFV TMSDVDELNE
310 320
AISALDGQNL EGRAIRVNVA EERPPRRGY
Length:329
Mass (Da):35,787
Last modified:February 1, 1994 - v1
Checksum:i4FD02DA48600A593
GO
Isoform 2 (identifier: Q04836-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-14: Missing.

Note: No experimental confirmation available.
Show »
Length:315
Mass (Da):34,401
Checksum:iD2F7E59ACB32FE0D
GO
Isoform 3 (identifier: Q04836-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-167: Missing.

Note: No experimental confirmation available.
Show »
Length:162
Mass (Da):18,318
Checksum:i0667BB1D2CBCCACD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti109 – 123Missing in BAA06520 (PubMed:7894017).CuratedAdd BLAST15
Sequence conflicti109 – 123Missing in BAA06521 (PubMed:7894017).CuratedAdd BLAST15
Sequence conflicti327R → H in AAK95304 (PubMed:14593172).Curated1
Sequence conflicti327R → H in AAN28804 (PubMed:14593172).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0188801 – 167Missing in isoform 3. CuratedAdd BLAST167
Alternative sequenceiVSP_0188791 – 14Missing in isoform 2. CuratedAdd BLAST14

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65255 mRNA Translation: CAA46347.1
M94554 mRNA Translation: AAA32860.1
U08467 Unassigned RNA Translation: AAA18378.1
U08467 Unassigned RNA Translation: AAA18379.1
U08467 Unassigned RNA Translation: AAA18380.1
D31712 Genomic DNA Translation: BAA06520.1
D31713 mRNA Translation: BAA06521.1
AL035356 Genomic DNA Translation: CAA22986.1
AL161562 Genomic DNA Translation: CAB79387.1
CP002687 Genomic DNA Translation: AEE84954.1
CP002687 Genomic DNA Translation: ANM66089.1
AF410318 mRNA Translation: AAK95304.1
AY143865 mRNA Translation: AAN28804.1
PIRiS28057
S53492
RefSeqiNP_001328008.1, NM_001341706.1 [Q04836-1]
NP_194208.1, NM_118610.4 [Q04836-1]
UniGeneiAt.38112
At.70990

Genome annotation databases

EnsemblPlantsiAT4G24770.1; AT4G24770.1; AT4G24770 [Q04836-1]
AT4G24770.2; AT4G24770.2; AT4G24770 [Q04836-1]
GeneIDi828579
GrameneiAT4G24770.1; AT4G24770.1; AT4G24770 [Q04836-1]
AT4G24770.2; AT4G24770.2; AT4G24770 [Q04836-1]
KEGGiath:AT4G24770

Keywords - Coding sequence diversityi

Alternative initiation, RNA editing

Similar proteinsi

Entry informationi

Entry nameiCP31A_ARATH
AccessioniPrimary (citable) accession number: Q04836
Secondary accession number(s): P92871
, Q03394, Q43350, Q94EH5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 23, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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