Uncharacterized membrane protein YMR253C - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q04835 (YM87_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 85. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Uncharacterized membrane protein YMR253C

Gene names

Ordered Locus Names:	YMR253C
ORF Names:	YM9920.07C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	414 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location

Membrane; Multi-pass membrane protein. Note: Localizes to cytoplasmic punctate structures. Ref.4

Sequence similarities

Contains 2 EamA domains.

To yeast YPL264c.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from direct assay Ref.4. Source: SGD integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 414

414

Uncharacterized membrane protein YMR253C

PRO_0000203340

Regions

Topological domain

1 – 66

Lumenal Potential

Transmembrane

67 – 87

Helical; Potential

Topological domain

88 – 106

Cytoplasmic Potential

Transmembrane

107 – 127

Helical; Potential

Topological domain

128 – 144

Lumenal Potential

Transmembrane

145 – 167

Helical; Potential

Topological domain

168 – 171

Cytoplasmic Potential

Transmembrane

172 – 191

Helical; Potential

Topological domain

192 – 199

Lumenal Potential

Transmembrane

200 – 220

Helical; Potential

Topological domain

221 – 241

Cytoplasmic Potential

Transmembrane

242 – 262

Helical; Potential

Topological domain

263 – 269

Lumenal Potential

Transmembrane

270 – 290

Helical; Potential

Topological domain

291 – 307

Cytoplasmic Potential

Transmembrane

308 – 328

Helical; Potential

Topological domain

329 – 357

Lumenal Potential

Transmembrane

358 – 378

Helical; Potential

Topological domain

379 – 414

Cytoplasmic Potential

Domain

78 – 215

138

EamA 1

Domain

253 – 379

127

EamA 2

Amino acid modifications

Modified residue

Phosphoserine Ref.6 Ref.7

Modified residue

Phosphoserine Ref.7

Experimental info

Sequence conflict

278

F → L in AAU09774. Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

Q04835 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: E0EBD0F3BE4CC8AB
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FASTA

414

46,659

        10         20         30         40         50         60 
MNPSVPKVMK RENNTHLLVS KEMNDTSLQL PSTTRSLSPK ESNSNEDFNV DGNETTLQRI 

        70         80         90        100        110        120 
SKDYLKPNIG LVLLTVSYFF NSAMVVSTKV LENDPDDIAN DRQIKPLQIL LVRMVITYIG 

       130        140        150        160        170        180 
TLIYMYINKS TISDVPFGKP EVRKWLVLRG CTGFFGVFGM YYSLMYLTIS DAVLITFLAP 

       190        200        210        220        230        240 
SLTIFLSWVI LRERFTKVEA LGSLISLLGV VLIVRPSFLF GTPELTDSSS QIVESSDPKS 

       250        260        270        280        290        300 
RLIATLVGLW GVLGMSCVYI IIRYIGKRAH AIMSVSYFSL ITAIVSFIGI NTIPSMKFQI 

       310        320        330        340        350        360 
PHSKKQWILF GNLGVSGFIF QLLLTMGIQR ERAGRGSLMT YTQLLYAVFW DVALYKHWPN 

       370        380        390        400        410 
IWSWIGMIII ISATLWVIRI RAANNETTAK DLTPIIDDEE NSIPLTEFDL SDSK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42 AND SER-44, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z48639 Genomic DNA. Translation: CAA88580.1. AY723857 Genomic DNA. Translation: AAU09774.1. BK006946 Genomic DNA. Translation: DAA10154.1.
PIR	S53075.
RefSeq	NP_013980.1. NM_001182760.1.
3D structure databases
ProteinModelPortal	Q04835.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5067N.
IntAct	Q04835. 4 interactions.
MINT	MINT-563831.
STRING	4932.YMR253C.
Proteomic databases
PaxDb	Q04835.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR253C; YMR253C; YMR253C.
GeneID	855295.
KEGG	sce:YMR253C.
Organism-specific databases
CYGD	YMR253c.
SGD	S000004866. YMR253C.
Phylogenomic databases
eggNOG	COG0697.
GeneTree	ENSGT00640000091521.
HOGENOM	HOG000248677.
OMA	YNIANIP.
OrthoDB	EOG4HTD25.
Gene expression databases
Genevestigator	Q04835.
GermOnline	YMR253C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000620. DMT. [Graphical view]
Pfam	PF00892. EamA. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	978952.

Entry information

Entry name

YM87_YEAST

Accession

Primary (citable) accession number: Q04835
Secondary accession number(s): D6W080, Q66R30

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents