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Q04835 (YM87_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Uncharacterized membrane protein YMR253C
Gene names
Ordered Locus Names:YMR253C
ORF Names:YM9920.07C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length414 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Membrane; Multi-pass membrane protein. Note: Localizes to cytoplasmic punctate structures. Ref.4

Sequence similarities

Contains 2 EamA domains.

To yeast YPL264c.

Ontologies

Keywords
   Cellular componentMembrane
   DomainRepeat
Transmembrane
Transmembrane helix
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 414414Uncharacterized membrane protein YMR253C
PRO_0000203340

Regions

Topological domain1 – 6666Lumenal Potential
Transmembrane67 – 8721Helical; Potential
Topological domain88 – 10619Cytoplasmic Potential
Transmembrane107 – 12721Helical; Potential
Topological domain128 – 14417Lumenal Potential
Transmembrane145 – 16723Helical; Potential
Topological domain168 – 1714Cytoplasmic Potential
Transmembrane172 – 19120Helical; Potential
Topological domain192 – 1998Lumenal Potential
Transmembrane200 – 22021Helical; Potential
Topological domain221 – 24121Cytoplasmic Potential
Transmembrane242 – 26221Helical; Potential
Topological domain263 – 2697Lumenal Potential
Transmembrane270 – 29021Helical; Potential
Topological domain291 – 30717Cytoplasmic Potential
Transmembrane308 – 32821Helical; Potential
Topological domain329 – 35729Lumenal Potential
Transmembrane358 – 37821Helical; Potential
Topological domain379 – 41436Cytoplasmic Potential
Domain78 – 215138EamA 1
Domain253 – 379127EamA 2

Experimental info

Sequence conflict2781F → L in AAU09774. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q04835 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: E0EBD0F3BE4CC8AB

FASTA41446,659
        10         20         30         40         50         60 
MNPSVPKVMK RENNTHLLVS KEMNDTSLQL PSTTRSLSPK ESNSNEDFNV DGNETTLQRI 

        70         80         90        100        110        120 
SKDYLKPNIG LVLLTVSYFF NSAMVVSTKV LENDPDDIAN DRQIKPLQIL LVRMVITYIG 

       130        140        150        160        170        180 
TLIYMYINKS TISDVPFGKP EVRKWLVLRG CTGFFGVFGM YYSLMYLTIS DAVLITFLAP 

       190        200        210        220        230        240 
SLTIFLSWVI LRERFTKVEA LGSLISLLGV VLIVRPSFLF GTPELTDSSS QIVESSDPKS 

       250        260        270        280        290        300 
RLIATLVGLW GVLGMSCVYI IIRYIGKRAH AIMSVSYFSL ITAIVSFIGI NTIPSMKFQI 

       310        320        330        340        350        360 
PHSKKQWILF GNLGVSGFIF QLLLTMGIQR ERAGRGSLMT YTQLLYAVFW DVALYKHWPN 

       370        380        390        400        410 
IWSWIGMIII ISATLWVIRI RAANNETTAK DLTPIIDDEE NSIPLTEFDL SDSK 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[6]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z48639 Genomic DNA. Translation: CAA88580.1.
AY723857 Genomic DNA. Translation: AAU09774.1.
BK006946 Genomic DNA. Translation: DAA10154.1.
PIRS53075.
RefSeqNP_013980.1. NM_001182760.1.

3D structure databases

ProteinModelPortalQ04835.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35432. 9 interactions.
DIPDIP-5067N.
IntActQ04835. 4 interactions.
MINTMINT-563831.
STRING4932.YMR253C.

Proteomic databases

MaxQBQ04835.
PaxDbQ04835.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR253C; YMR253C; YMR253C.
GeneID855295.
KEGGsce:YMR253C.

Organism-specific databases

CYGDYMR253c.
SGDS000004866. YMR253C.

Phylogenomic databases

eggNOGCOG0697.
GeneTreeENSGT00730000111035.
HOGENOMHOG000248677.
OMAAFIAWVA.
OrthoDBEOG744TKH.

Enzyme and pathway databases

BioCycYEAST:G3O-32930-MONOMER.

Gene expression databases

GenevestigatorQ04835.

Family and domain databases

InterProIPR000620. DMT.
[Graphical view]
PfamPF00892. EamA. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio978952.

Entry information

Entry nameYM87_YEAST
AccessionPrimary (citable) accession number: Q04835
Secondary accession number(s): D6W080, Q66R30
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 14, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families