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Q04830

- ENAN_BPK1F

UniProt

Q04830 - ENAN_BPK1F

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Protein
Endo-N-acetylneuraminidase
Gene
N/A
Organism
Enterobacteria phage K1F (Bacteriophage K1F)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Responsible for initial absorption of the phage to the host bacterium. Degradation of the alpha-2,8-linked polysialic acid K1 capsule.

Catalytic activityi

Endohydrolysis of (2->8)-alpha-sialosyl linkages in oligo- or poly(sialic) acids.

GO - Molecular functioni

  1. endo-alpha-(2,8)-sialidase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BRENDAi3.2.1.129. 716.

    Protein family/group databases

    CAZyiGH58. Glycoside Hydrolase Family 58.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endo-N-acetylneuraminidase (EC:3.2.1.129)
    Short name:
    Endo-N
    Alternative name(s):
    Endosialidase
    G102
    OrganismiEnterobacteria phage K1F (Bacteriophage K1F)
    Taxonomic identifieri344021 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeAutographivirinaeT7likevirus
    Virus hostiEscherichia coli [TaxID: 562]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed; by host1 Publication
    Chaini2 – 920919Endo-N-acetylneuraminidase
    PRO_0000057709Add
    BLAST

    Interactioni

    Subunit structurei

    Homotrimer.

    Structurei

    Secondary structure

    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi249 – 2535
    Helixi255 – 26410
    Beta strandi277 – 2793
    Helixi286 – 2883
    Beta strandi289 – 2913
    Beta strandi293 – 2964
    Beta strandi304 – 3074
    Beta strandi311 – 3188
    Beta strandi323 – 3275
    Beta strandi334 – 3363
    Beta strandi339 – 35214
    Beta strandi356 – 3649
    Beta strandi373 – 3764
    Turni380 – 3845
    Beta strandi385 – 3884
    Beta strandi392 – 3954
    Beta strandi398 – 40710
    Turni408 – 4103
    Beta strandi413 – 42210
    Beta strandi426 – 4316
    Beta strandi433 – 4353
    Beta strandi441 – 4455
    Beta strandi456 – 4616
    Beta strandi469 – 4724
    Beta strandi475 – 4784
    Beta strandi481 – 4855
    Beta strandi500 – 5056
    Beta strandi513 – 5164
    Beta strandi524 – 5318
    Beta strandi533 – 5353
    Beta strandi537 – 5437
    Beta strandi545 – 5484
    Beta strandi550 – 5567
    Turni557 – 5615
    Beta strandi567 – 5704
    Helixi573 – 5753
    Beta strandi579 – 5879
    Beta strandi590 – 5989
    Beta strandi606 – 6127
    Beta strandi618 – 6214
    Beta strandi633 – 6364
    Beta strandi639 – 6457
    Beta strandi667 – 6748
    Turni675 – 6773
    Beta strandi686 – 6905
    Beta strandi696 – 6983
    Beta strandi702 – 7109
    Beta strandi713 – 7219
    Turni728 – 7347
    Beta strandi746 – 7538
    Beta strandi772 – 7743
    Beta strandi784 – 7863
    Beta strandi790 – 7923
    Beta strandi796 – 8038
    Turni808 – 8114
    Beta strandi812 – 8176
    Beta strandi819 – 8268
    Helixi833 – 8353
    Beta strandi837 – 84711
    Helixi848 – 8503
    Beta strandi853 – 8575
    Beta strandi861 – 8633
    Beta strandi867 – 8704
    Beta strandi872 – 8798
    Beta strandi884 – 8896
    Beta strandi891 – 8933
    Beta strandi901 – 9055

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1V0EX-ray1.90A/B/C/D/E/F246-910[»]
    1V0FX-ray2.55A/B/C/D/E/F246-910[»]
    3GVJX-ray1.48A246-910[»]
    3GVKX-ray1.84A/B/C246-910[»]
    3GVLX-ray1.41A246-910[»]
    3GW6X-ray2.60A/B/C/D/E/F790-913[»]
    3JU4X-ray0.98A246-910[»]
    ProteinModelPortaliQ04830.
    SMRiQ04830. Positions 246-910.

    Miscellaneous databases

    EvolutionaryTraceiQ04830.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati360 – 37112BNR 1
    Add
    BLAST
    Repeati496 – 5038BNR 2
    Repeati608 – 61912BNR 3
    Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi198 – 21619Gly-rich (hinge)
    Add
    BLAST

    Sequence similaritiesi

    Contains 3 BNR repeats.

    Keywords - Domaini

    Repeat

    Family and domain databases

    Gene3Di2.120.10.10. 2 hits.
    2.40.30.20. 1 hit.
    3.30.750.60. 1 hit.
    4.10.1090.10. 1 hit.
    InterProiIPR023366. ATPase_asu-like.
    IPR024427. Endosialidase_beta_barrel.
    IPR024428. Endosialidase_beta_prop.
    IPR024430. Endosialidase_C_dom.
    IPR024429. Endosialidase_N-extension.
    IPR001724. Glycl_Hydrolase_58.
    IPR005604. Phage_T7_tail_fibre.
    IPR011040. Sialidases.
    [Graphical view]
    PfamiPF12195. End_beta_barrel. 1 hit.
    PF12217. End_beta_propel. 1 hit.
    PF12218. End_N_terminal. 1 hit.
    PF12219. End_tail_spike. 1 hit.
    PF03906. Phage_T7_tail. 1 hit.
    [Graphical view]
    PRINTSiPR00849. GLHYDRLASE58.
    SUPFAMiSSF50939. SSF50939. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q04830-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTITQFPSG NTQYRIEFDY LARTFVVVTL VNSSNPTLNR VLEVGRDYRF    50
    LNPTMIEMLV DQSGFDIVRI HRQTGTDLVV DFRNGSVLTA SDLTTAELQA 100
    IHIAEEGRDQ TVDLAKEYAD AAGSSAGNAK DSEDEARRIA ESIRAAGLIG 150
    YMTRRSFEKG YNVTTWSEVL LWEEDGDYYR WDGTLPKNVP AGSTPETSGG 200
    IGLGAWVSVG DAALRSQISN PEGAILYPEL HRARWLDEKD ARGWGAKGDG 250
    VTDDTAALTS ALNDTPVGQK INGNGKTYKV TSLPDISRFI NTRFVYERIP 300
    GQPLYYASEE FVQGELFKIT DTPYYNAWPQ DKAFVYENVI YAPYMGSDRH 350
    GVSRLHVSWV KSGDDGQTWS TPEWLTDLHP DYPTVNYHCM SMGVCRNRLF 400
    AMIETRTLAK NALTNCALWD RPMSRSLHLT GGITKAANQR YATIHVPDHG 450
    LFVGDFVNFS NSAVTGVSGD MTVATVIDKD NFTVLTPNQQ TSDLNNAGKN 500
    WHMGTSFHKS PWRKTDLGLI PSVTEVHSFA TIDNNGFAMG YHQGDVAPRE 550
    VGLFYFPDAF NSPSNYVRRQ IPSEYEPDAS EPCIKYYDGV LYLITRGTRG 600
    DRLGSSLHRS RDIGQTWESL RFPHNVHRTT LPFAKVGDDL IMFGSERAEN 650
    EWEAGAPDDR YKASYPRTFY ARLNVNNWNA DDIEWVNITD QIYQGGIVNS 700
    GVGVGSVVVK DNYIYYMFGG EDHFNPWTYG DNSAKDPFKS DGHPSDLYCY 750
    KMKIGPDNRV SRDFRYGAVP NRAVPVFFDT NGVRTVPAPM EFTGDLGLGH 800
    VTIRASTSSN IRSEVLMEGE YGFIGKSIPT DNPAGQRIIF CGGEGTSSTT 850
    GAQITLYGAN NTDSRRIVYN GDEHLFQSAD VKPYNDNVTA LGGPSNRFTT 900
    AYLGSNPIVT SNGGGGKQSR 920
    Length:920
    Mass (Da):102,013
    Last modified:January 23, 2007 - v3
    Checksum:i62B77613F55884B3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63657 Unassigned DNA. Translation: AAC37340.1.
    PIRiA36887.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63657 Unassigned DNA. Translation: AAC37340.1 .
    PIRi A36887.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1V0E X-ray 1.90 A/B/C/D/E/F 246-910 [» ]
    1V0F X-ray 2.55 A/B/C/D/E/F 246-910 [» ]
    3GVJ X-ray 1.48 A 246-910 [» ]
    3GVK X-ray 1.84 A/B/C 246-910 [» ]
    3GVL X-ray 1.41 A 246-910 [» ]
    3GW6 X-ray 2.60 A/B/C/D/E/F 790-913 [» ]
    3JU4 X-ray 0.98 A 246-910 [» ]
    ProteinModelPortali Q04830.
    SMRi Q04830. Positions 246-910.
    ModBasei Search...

    Protein family/group databases

    CAZyi GH58. Glycoside Hydrolase Family 58.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BRENDAi 3.2.1.129. 716.

    Miscellaneous databases

    EvolutionaryTracei Q04830.

    Family and domain databases

    Gene3Di 2.120.10.10. 2 hits.
    2.40.30.20. 1 hit.
    3.30.750.60. 1 hit.
    4.10.1090.10. 1 hit.
    InterProi IPR023366. ATPase_asu-like.
    IPR024427. Endosialidase_beta_barrel.
    IPR024428. Endosialidase_beta_prop.
    IPR024430. Endosialidase_C_dom.
    IPR024429. Endosialidase_N-extension.
    IPR001724. Glycl_Hydrolase_58.
    IPR005604. Phage_T7_tail_fibre.
    IPR011040. Sialidases.
    [Graphical view ]
    Pfami PF12195. End_beta_barrel. 1 hit.
    PF12217. End_beta_propel. 1 hit.
    PF12218. End_N_terminal. 1 hit.
    PF12219. End_tail_spike. 1 hit.
    PF03906. Phage_T7_tail. 1 hit.
    [Graphical view ]
    PRINTSi PR00849. GLHYDRLASE58.
    SUPFAMi SSF50939. SSF50939. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete nucleotide sequence of the bacteriophage K1F tail gene encoding endo-N-acylneuraminidase (endo-N) and comparison to an endo-N homolog in bacteriophage PK1E."
      Petter J.G., Vimr E.R.
      J. Bacteriol. 175:4354-4363(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE, PROTEIN SEQUENCE OF 2-31 AND 917-920.

    Entry informationi

    Entry nameiENAN_BPK1F
    AccessioniPrimary (citable) accession number: Q04830
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: January 23, 2007
    Last modified: October 16, 2013
    This is version 82 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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