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Q04830 (ENAN_BPK1F) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Endo-N-acetylneuraminidase
Short name=Endo-N
EC=3.2.1.129
Alternative name(s):
Endosialidase
G102
OrganismEnterobacteria phage K1F (Bacteriophage K1F)
Taxonomic identifier344021 [NCBI]
Taxonomic lineageViruses › dsDNA viruses, no RNA stage › Caudovirales › Podoviridae › Autographivirinae › T7-like viruses
Virus hostEscherichia coli [TaxID: 562]

Protein attributes

Sequence length920 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Responsible for initial absorption of the phage to the host bacterium. Degradation of the alpha-2,8-linked polysialic acid K1 capsule.

Catalytic activity

Endohydrolysis of (2->8)-alpha-sialosyl linkages in oligo- or poly(sialic) acids.

Subunit structure

Homotrimer.

Sequence similarities

Belongs to the glycosyl hydrolase 58 family.

Contains 3 BNR repeats.

Ontologies

Keywords
   DomainRepeat
   Molecular functionGlycosidase
Hydrolase
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Molecular functionendo-alpha-(2,8)-sialidase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed; by host Ref.1
Chain2 – 920919Endo-N-acetylneuraminidase
PRO_0000057709

Regions

Repeat360 – 37112BNR 1
Repeat496 – 5038BNR 2
Repeat608 – 61912BNR 3
Compositional bias198 – 21619Gly-rich (hinge)

Secondary structure

1 ................................................................................................................................ 920
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q04830 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 62B77613F55884B3

FASTA920102,013
        10         20         30         40         50         60 
MSTITQFPSG NTQYRIEFDY LARTFVVVTL VNSSNPTLNR VLEVGRDYRF LNPTMIEMLV 

        70         80         90        100        110        120 
DQSGFDIVRI HRQTGTDLVV DFRNGSVLTA SDLTTAELQA IHIAEEGRDQ TVDLAKEYAD 

       130        140        150        160        170        180 
AAGSSAGNAK DSEDEARRIA ESIRAAGLIG YMTRRSFEKG YNVTTWSEVL LWEEDGDYYR 

       190        200        210        220        230        240 
WDGTLPKNVP AGSTPETSGG IGLGAWVSVG DAALRSQISN PEGAILYPEL HRARWLDEKD 

       250        260        270        280        290        300 
ARGWGAKGDG VTDDTAALTS ALNDTPVGQK INGNGKTYKV TSLPDISRFI NTRFVYERIP 

       310        320        330        340        350        360 
GQPLYYASEE FVQGELFKIT DTPYYNAWPQ DKAFVYENVI YAPYMGSDRH GVSRLHVSWV 

       370        380        390        400        410        420 
KSGDDGQTWS TPEWLTDLHP DYPTVNYHCM SMGVCRNRLF AMIETRTLAK NALTNCALWD 

       430        440        450        460        470        480 
RPMSRSLHLT GGITKAANQR YATIHVPDHG LFVGDFVNFS NSAVTGVSGD MTVATVIDKD 

       490        500        510        520        530        540 
NFTVLTPNQQ TSDLNNAGKN WHMGTSFHKS PWRKTDLGLI PSVTEVHSFA TIDNNGFAMG 

       550        560        570        580        590        600 
YHQGDVAPRE VGLFYFPDAF NSPSNYVRRQ IPSEYEPDAS EPCIKYYDGV LYLITRGTRG 

       610        620        630        640        650        660 
DRLGSSLHRS RDIGQTWESL RFPHNVHRTT LPFAKVGDDL IMFGSERAEN EWEAGAPDDR 

       670        680        690        700        710        720 
YKASYPRTFY ARLNVNNWNA DDIEWVNITD QIYQGGIVNS GVGVGSVVVK DNYIYYMFGG 

       730        740        750        760        770        780 
EDHFNPWTYG DNSAKDPFKS DGHPSDLYCY KMKIGPDNRV SRDFRYGAVP NRAVPVFFDT 

       790        800        810        820        830        840 
NGVRTVPAPM EFTGDLGLGH VTIRASTSSN IRSEVLMEGE YGFIGKSIPT DNPAGQRIIF 

       850        860        870        880        890        900 
CGGEGTSSTT GAQITLYGAN NTDSRRIVYN GDEHLFQSAD VKPYNDNVTA LGGPSNRFTT 

       910        920 
AYLGSNPIVT SNGGGGKQSR

« Hide

References

[1]"Complete nucleotide sequence of the bacteriophage K1F tail gene encoding endo-N-acylneuraminidase (endo-N) and comparison to an endo-N homolog in bacteriophage PK1E."
Petter J.G., Vimr E.R.
J. Bacteriol. 175:4354-4363(1993) [PubMed: 8331067] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE, PROTEIN SEQUENCE OF 2-31 AND 917-920.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M63657 Unassigned DNA. Translation: AAC37340.1.
PIRA36887.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1V0EX-ray1.90A/B/C/D/E/F246-910[»]
1V0FX-ray2.55A/B/C/D/E/F246-910[»]
3GVJX-ray1.48A246-910[»]
3GVKX-ray1.84A/B/C246-910[»]
3GVLX-ray1.41A246-910[»]
3GW6X-ray2.60A/B/C/D/E/F790-913[»]
3JU4X-ray0.98A246-910[»]
ProteinModelPortalQ04830.
SMRQ04830. Positions 246-910.
ModBaseSearch...

Protein family/group databases

CAZyGH58. Glycoside Hydrolase Family 58.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BRENDA3.2.1.129. 716.

Family and domain databases

InterProIPR023366. ATPase_F1/A1-cplx_a_su_N.
IPR024427. Endosialidase_beta_barrel.
IPR024428. Endosialidase_beta_prop.
IPR024430. Endosialidase_C.
IPR024429. Endosialidase_N-extension.
IPR001724. Glyco_hydro_58.
IPR011040. Neuraminidase.
IPR005604. Phage_T7_tail_fibre.
[Graphical view]
Gene3DG3DSA:2.40.30.20. G3DSA:2.40.30.20. 1 hit.
G3DSA:3.30.750.60. G3DSA:3.30.750.60. 1 hit.
G3DSA:4.10.1090.10. G3DSA:4.10.1090.10. 1 hit.
G3DSA:2.120.10.10. Neuraminidase. 2 hits.
PfamPF12195. End_beta_barrel. 1 hit.
PF12217. End_beta_propel. 1 hit.
PF12218. End_N_terminal. 1 hit.
PF12219. End_tail_spike. 1 hit.
PF03906. Phage_T7_tail. 1 hit.
[Graphical view]
PRINTSPR00849. GLHYDRLASE58.
SUPFAMSSF50939. Sialidase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameENAN_BPK1F
AccessionPrimary (citable) accession number: Q04830
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: December 14, 2011
This is version 75 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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