Q04827 (CCND2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
G1/S-specific cyclin-D2

Alternative name(s):
Vin-1 proto-oncogene

Gene names

Name:	Ccnd2
Synonyms:	Vin-1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex By similarity.
Subunit structure	Interacts with either CDK4 or CDK6 protein kinase to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Component of the ternary complex cyclin D/CDK4/CDKN1B required for nuclear translocation and modulation of CDK4-mediated kinase activity By similarity.
Subcellular location	Nucleus By similarity. Cytoplasm By similarity. Membrane By similarity. Note: Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members By similarity.
Sequence similarities	Belongs to the cyclin family. Cyclin D subfamily. Contains 1 cyclin N-terminal domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Cytoplasm Membrane Nucleus
Molecular function	Cyclin
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	G1/S transition of mitotic cell cycle Inferred from mutant phenotype. Source: RGD cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular response to insulin stimulus Inferred from expression pattern. Source: RGD liver development Inferred from expression pattern. Source: RGD ovarian follicle development Inferred from expression pattern. Source: RGD positive regulation of epithelial cell proliferation Inferred from mutant phenotype. Source: RGD regulation of neuron differentiation Inferred from expression pattern. Source: RGD response to cAMP Inferred from expression pattern. Source: RGD response to estradiol stimulus Inferred from expression pattern. Source: RGD response to ethanol Inferred from expression pattern. Source: RGD response to organic cyclic compound Inferred from expression pattern. Source: RGD response to testosterone stimulus Inferred from expression pattern. Source: RGD spermatogenesis Inferred from expression pattern. Source: RGD
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein kinase binding Inferred from physical interaction. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	G1/S-specific cyclin-D2		PRO_0000080439
Regions
	Domain	26 – 151	126	Cyclin N-terminal
Experimental info
	Sequence conflict	68	1	E → G in BAA03815. Ref.2
	Sequence conflict	104	1	C → V in BAA03815. Ref.2
	Sequence conflict	232	1	T → A in BAA03815. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q04827 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 4B522BF4E9835FC1	FASTA	288	32,826
	10 20 30 40 50 60 MELLCCEVDP VRRAVPDRNL LEDRVLQNLL TIEERYLPQC SYFKCVQKDI QPYMRRMVAT 70 80 90 100 110 120 WMLEVCEEQK CEEEVFPLAM NYLDRFLAGV PTPKTHLQLL GAVCMFLASK LKETIPLTAE 130 140 150 160 170 180 KLCIYTDNSV KPQELLEWEL VVLGKLKWNL AAVTPHDFIE HILRKLPQQK EKLSLIRKHA 190 200 210 220 230 240 QTFIALCATD FKFAMYPPSM IATGSVGAAI CGLQQDEEVN ALTCDALTEL LTKITHTDVD 250 260 270 280 CLKACQEQIE AVLLNSLQQF RQEQHNGSKS VEDPDQATTP TDVRDVDL

References

[1]	"The Vin-1 gene, identified by provirus insertional mutagenesis, is the cyclin D2." Hanna Z., Jankowski M., Tremblay P., Jiang X.M., Milatovich A., Francke U., Jolicoeur P. Oncogene 8:1661-1666(1993) [PubMed: 8502486] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Induction of D2 and D3 cyclin-encoding genes during promotion of the G1/S transition by prolactin in rat Nb2 cells." Hosokawa Y., Onga T., Nakashima K. Gene 147:249-252(1994) [PubMed: 7926809] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L09752 mRNA. Translation: AAA41010.1. D16308 mRNA. Translation: BAA03815.1.
IPI	IPI00189816.
PIR	I58372. JC4011.
RefSeq	NP_071603.1. NM_022267.1.
UniGene	Rn.96083.
3D structure databases
ProteinModelPortal	Q04827.
ModBase	Search...
Protein-protein interaction databases
STRING	Q04827.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	64033.
KEGG	rno:64033.
UCSC	NM_022267. rat.
Organism-specific databases
CTD	894.
RGD	621083. Ccnd2.
Phylogenomic databases
eggNOG	maNOG11758.
HOVERGEN	HBG050837.
InParanoid	Q04827.
OrthoDB	EOG4JWVF1.
PhylomeDB	Q04827.
Gene expression databases
ArrayExpress	Q04827.
Genevestigator	Q04827.
GermOnline	ENSRNOG00000019939. Rattus norvegicus.
Family and domain databases
InterPro	IPR013763. Cyclin-like. IPR014400. Cyclin_A/B/D/E. IPR004367. Cyclin_C. IPR006671. Cyclin_N. [Graphical view]
Gene3D	G3DSA:1.10.472.10. Cyclin_related. 2 hits.
KO	K10151.
Pfam	PF02984. Cyclin_C. 1 hit. PF00134. Cyclin_N. 1 hit. [Graphical view]
PIRSF	PIRSF001771. Cyclin_A_B_D_E. 1 hit.
SMART	SM00385. CYCLIN. 1 hit. [Graphical view]
SUPFAM	SSF47954. Cyclin_like. 2 hits.
PROSITE	PS00292. CYCLINS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	612662.

Entry information

Entry name

CCND2_RAT

Accession

Primary (citable) accession number: Q04827

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	November 16, 2011
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program