Reviewed, UniProtKB/Swiss-Prot Q04820 (MDHC_ECHGR)

Names and origin

Protein names

Recommended name:
    Malate dehydrogenase, cytoplasmic
    EC=1.1.1.37

Gene names

Name:

MDH

Organism

Echinococcus granulosus

Taxonomic identifier

6210 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Platyhelminthes › Cestoda › Eucestoda › Cyclophyllidea › Taeniidae › Echinococcus

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	Malate dehydrogenase, cytoplasmic		PRO_0000113408
Regions
	Nucleotide binding	11 – 17	7	NAD By similarity
	Nucleotide binding	129 – 131	3	NAD By similarity
Sites
	Active site	187	1	Proton acceptor By similarity
	Binding site	92	1	Substrate By similarity
	Binding site	98	1	Substrate By similarity
	Binding site	105	1	NAD By similarity
	Binding site	112	1	NAD By similarity
	Binding site	131	1	Substrate By similarity
	Binding site	162	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q04820-1 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: 0EACE29A26F565E2	FASTA	332	36,651
	10 20 30 40 50 60 MPGPLRVLIT GAAGQIAYNL SNMVANGNLF GKDQQIILHL LDIPEAKTVL DGVVMELQDC 70 80 90 100 110 120 AFTVLAGIVP THCLKEAFTD IDVALMVGAM PRKQGMERRD LLSSNVKIFK EQGEALDKYA 130 140 150 160 170 180 KKTVKVLVVG NPANTNCLIM SKYAPSIPKE NFTALTRLDH NRAIYQVAAK AGVPNTCVKN 190 200 210 220 230 240 VCIWGNHSNK QFPDLSHAVV TKDGKQHPAK ELINDEKWVK EVFIPCVQNR GAAVIGLRKL 250 260 270 280 290 300 SRAASAAKAI VDQMRDWWFG TKEGEWVSMS VYSTGDHYGA PKDIYFSFPV TIKDGHYKVV 310 320 330 DGLSMDEWSR SLFNLSADEL VDEREVALAS FK

References

[1]	"Molecular cloning and characterization of an Echinococcus granulosus cDNA encoding malate dehydrogenase." Rodrigues J.J., Ferreira H.B., Zaha A. Mol. Biochem. Parasitol. 60:157-160(1993) [PubMed: 8366891] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Rodrigues J.J. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L08894 mRNA. Translation: AAC28239.1.
PIR	T09228.
3D structure databases
HSSP	HSSP built from PDB template 4MDH based on UniProtKB P11708.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.37. 1682.
Family and domain databases
InterPro	IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR011274. Malate_DH_NAD-dep_euk. IPR010945. Malate_DH_SF1. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23382. MDH_SF1. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
ProDom	PD003052. Mal_dehydrog. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01759. MalateDH-SF1. 1 hit. TIGR01758. MDH_euk_cyt. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDHC_ECHGR

Accession

Primary (citable) accession number: Q04820

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	December 15, 1998
Last modified:	June 16, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)