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Q04820 (MDHC_ECHGR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase, cytoplasmic

EC=1.1.1.37
Gene names
Name:MDH
OrganismEchinococcus granulosus (Hydatid tapeworm)
Taxonomic identifier6210 [NCBI]
Taxonomic lineageEukaryotaMetazoaPlatyhelminthesCestodaEucestodaCyclophyllideaTaeniidaeEchinococcus

Protein attributes

Sequence length332 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH.

Subunit structure

Homodimer.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 332332Malate dehydrogenase, cytoplasmic
PRO_0000113408

Regions

Nucleotide binding11 – 177NAD By similarity
Nucleotide binding129 – 1313NAD By similarity

Sites

Active site1871Proton acceptor By similarity
Binding site921Substrate By similarity
Binding site981Substrate By similarity
Binding site1051NAD By similarity
Binding site1121NAD By similarity
Binding site1311Substrate By similarity
Binding site1621Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q04820 [UniParc].

Last modified December 15, 1998. Version 2.
Checksum: 0EACE29A26F565E2

FASTA33236,651
        10         20         30         40         50         60 
MPGPLRVLIT GAAGQIAYNL SNMVANGNLF GKDQQIILHL LDIPEAKTVL DGVVMELQDC 

        70         80         90        100        110        120 
AFTVLAGIVP THCLKEAFTD IDVALMVGAM PRKQGMERRD LLSSNVKIFK EQGEALDKYA 

       130        140        150        160        170        180 
KKTVKVLVVG NPANTNCLIM SKYAPSIPKE NFTALTRLDH NRAIYQVAAK AGVPNTCVKN 

       190        200        210        220        230        240 
VCIWGNHSNK QFPDLSHAVV TKDGKQHPAK ELINDEKWVK EVFIPCVQNR GAAVIGLRKL 

       250        260        270        280        290        300 
SRAASAAKAI VDQMRDWWFG TKEGEWVSMS VYSTGDHYGA PKDIYFSFPV TIKDGHYKVV 

       310        320        330 
DGLSMDEWSR SLFNLSADEL VDEREVALAS FK 

« Hide

References

[1]"Molecular cloning and characterization of an Echinococcus granulosus cDNA encoding malate dehydrogenase."
Rodrigues J.J., Ferreira H.B., Zaha A.
Mol. Biochem. Parasitol. 60:157-160(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Rodrigues J.J.
Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L08894 mRNA. Translation: AAC28239.1.
PIRT09228.

3D structure databases

ProteinModelPortalQ04820.
SMRQ04820. Positions 4-328.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR23382. PTHR23382. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMSSF56327. SSF56327. 1 hit.
TIGRFAMsTIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMDHC_ECHGR
AccessionPrimary (citable) accession number: Q04820
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: December 15, 1998
Last modified: February 19, 2014
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families