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Q04806

- GTO3_YEAST

UniProt

Q04806 - GTO3_YEAST

Protein

Glutathione S-transferase omega-like 3

Gene

GTO3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Active as '1-Cys' thiol transferase against beta-hydroxyethyl disulfide (HED), as dehydroascorbate reductase and as dimethylarsinic acid reductase, while not active against the standard GST substrate 1-chloro-2,4-dinitrobenzene (CDNB).1 Publication

    Catalytic activityi

    RX + glutathione = HX + R-S-glutathione.1 Publication

    Kineticsi

    1. KM=1.40 mM for reduced glutathione (GSH)1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei46 – 461By similarity

    GO - Molecular functioni

    1. glutathione transferase activity Source: SGD

    GO - Biological processi

    1. glutathione metabolic process Source: SGD

    Keywords - Molecular functioni

    Transferase

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32927-MONOMER.
    SABIO-RKQ04806.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase omega-like 3 (EC:2.5.1.18)
    Gene namesi
    Name:GTO3
    Ordered Locus Names:YMR251W
    ORF Names:YM9920.05
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR251w.
    SGDiS000004863. GTO3.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 366366Glutathione S-transferase omega-like 3PRO_0000203338Add
    BLAST

    Proteomic databases

    PaxDbiQ04806.

    Expressioni

    Inductioni

    Up-regulated by tert-butyl hydroperoxide (t-BOOH) in an MSN2/4-dependent manner.1 Publication

    Gene expression databases

    GenevestigatoriQ04806.

    Interactioni

    Protein-protein interaction databases

    BioGridi35429. 4 interactions.
    DIPiDIP-6643N.
    IntActiQ04806. 1 interaction.
    MINTiMINT-672625.
    STRINGi4932.YMR251W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ04806.
    SMRiQ04806. Positions 13-365.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini197 – 349153GST C-terminalAdd
    BLAST

    Sequence similaritiesi

    Belongs to the GST superfamily. Omega family.Curated
    Contains 1 GST C-terminal domain.Curated

    Phylogenomic databases

    eggNOGiCOG0435.
    GeneTreeiENSGT00530000065151.
    HOGENOMiHOG000245143.
    OMAiWISADNT.
    OrthoDBiEOG722JK6.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 2 hits.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR016639. GST.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PANTHERiPTHR32419. PTHR32419. 1 hit.
    PfamiPF13409. GST_N_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF015753. GST. 1 hit.
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 2 hits.
    PROSITEiPS50405. GST_CTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q04806-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEKSASNNK AEFKRQSSPF REIISADHPI YKPAKGRYWL YVALPCPWAQ    50
    RTLITRALKG LAPIIGCSVA HWHLDDKGWR FLEEGDGKTN ERHWFDIAGG 100
    ISSVNLNTST PVANIPNNAH RLLVDGTDEP HYGYKRLSDF YFKTKPDYKG 150
    RFTVPVLWDL ETCTIVNNES SDIIGIMNSA AFDEFVGEEY RQVRLVPRSL 200
    EAQITEFNSW VYDKINNGVY KAGFAECAEV YEREVTSLFQ YLDKLENLLD 250
    KKYTDLEAEY GKNNKDKILD RYFAIGDTLT EADVRLYPTI VRFDVVYHQH 300
    FKCNLATIRD DYSRIHTWLK NIYWRHEAFQ RTTDFTHIKL GYTRSQPRVN 350
    PIGITPLGPK PDIRPP 366
    Length:366
    Mass (Da):42,403
    Last modified:November 1, 1997 - v1
    Checksum:iC0BF9F2D3AB5C15B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z48639 Genomic DNA. Translation: CAA88578.1.
    AY692580 Genomic DNA. Translation: AAT92599.1.
    BK006946 Genomic DNA. Translation: DAA10151.1.
    PIRiS53073.
    RefSeqiNP_013977.1. NM_001182757.1.

    Genome annotation databases

    EnsemblFungiiYMR251W; YMR251W; YMR251W.
    GeneIDi855292.
    KEGGisce:YMR251W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z48639 Genomic DNA. Translation: CAA88578.1 .
    AY692580 Genomic DNA. Translation: AAT92599.1 .
    BK006946 Genomic DNA. Translation: DAA10151.1 .
    PIRi S53073.
    RefSeqi NP_013977.1. NM_001182757.1.

    3D structure databases

    ProteinModelPortali Q04806.
    SMRi Q04806. Positions 13-365.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35429. 4 interactions.
    DIPi DIP-6643N.
    IntActi Q04806. 1 interaction.
    MINTi MINT-672625.
    STRINGi 4932.YMR251W.

    Proteomic databases

    PaxDbi Q04806.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR251W ; YMR251W ; YMR251W .
    GeneIDi 855292.
    KEGGi sce:YMR251W.

    Organism-specific databases

    CYGDi YMR251w.
    SGDi S000004863. GTO3.

    Phylogenomic databases

    eggNOGi COG0435.
    GeneTreei ENSGT00530000065151.
    HOGENOMi HOG000245143.
    OMAi WISADNT.
    OrthoDBi EOG722JK6.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32927-MONOMER.
    SABIO-RK Q04806.

    Miscellaneous databases

    NextBioi 978943.

    Gene expression databases

    Genevestigatori Q04806.

    Family and domain databases

    Gene3Di 1.20.1050.10. 1 hit.
    3.40.30.10. 2 hits.
    InterProi IPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR016639. GST.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    PANTHERi PTHR32419. PTHR32419. 1 hit.
    Pfami PF13409. GST_N_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF015753. GST. 1 hit.
    SUPFAMi SSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 2 hits.
    PROSITEi PS50405. GST_CTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Saccharomyces cerevisiae cells have three Omega class glutathione S-transferases acting as 1-Cys thiol transferases."
      Garcera A., Barreto L., Piedrafita L., Tamarit J., Herrero E.
      Biochem. J. 398:187-196(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
    5. "A peroxisomal glutathione transferase of Saccharomyces cerevisiae is functionally related to sulfur amino acid metabolism."
      Barreto L., Garcera A., Jansson K., Sunnerhagen P., Herrero E.
      Eukaryot. Cell 5:1748-1759(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INDUCTION.

    Entry informationi

    Entry nameiGTO3_YEAST
    AccessioniPrimary (citable) accession number: Q04806
    Secondary accession number(s): D6W077
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3