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Protein

Glutathione S-transferase omega-like 3

Gene

GTO3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Active as '1-Cys' thiol transferase against beta-hydroxyethyl disulfide (HED), as dehydroascorbate reductase and as dimethylarsinic acid reductase, while not active against the standard GST substrate 1-chloro-2,4-dinitrobenzene (CDNB).1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.1 Publication

Kineticsi

  1. KM=1.40 mM for reduced glutathione (GSH)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei46By similarity1

    GO - Molecular functioni

    • glutathione transferase activity Source: SGD

    GO - Biological processi

    • glutathione metabolic process Source: SGD

    Keywordsi

    Molecular functionTransferase

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32927-MONOMER
    SABIO-RKiQ04806

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase omega-like 3 (EC:2.5.1.18)
    Gene namesi
    Name:GTO3
    Ordered Locus Names:YMR251W
    ORF Names:YM9920.05
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XIII

    Organism-specific databases

    EuPathDBiFungiDB:YMR251W
    SGDiS000004863 GTO3

    Subcellular locationi

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002033381 – 366Glutathione S-transferase omega-like 3Add BLAST366

    Proteomic databases

    PaxDbiQ04806
    PRIDEiQ04806

    Expressioni

    Inductioni

    Up-regulated by tert-butyl hydroperoxide (t-BOOH) in an MSN2/4-dependent manner.1 Publication

    Interactioni

    Protein-protein interaction databases

    BioGridi35429, 29 interactors
    DIPiDIP-6643N
    IntActiQ04806, 4 interactors
    STRINGi4932.YMR251W

    Structurei

    3D structure databases

    ProteinModelPortaliQ04806
    SMRiQ04806
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini197 – 349GST C-terminalAdd BLAST153

    Sequence similaritiesi

    Belongs to the GST superfamily. Omega family.Curated

    Phylogenomic databases

    GeneTreeiENSGT00530000065151
    HOGENOMiHOG000245143
    InParanoidiQ04806
    OMAiSCPWATR
    OrthoDBiEOG092C3FEI

    Family and domain databases

    InterProiView protein in InterPro
    IPR010987 Glutathione-S-Trfase_C-like
    IPR036282 Glutathione-S-Trfase_C_sf
    IPR004045 Glutathione_S-Trfase_N
    IPR016639 GST_Omega/GSH
    IPR036249 Thioredoxin-like_sf
    PANTHERiPTHR32419 PTHR32419, 1 hit
    PfamiView protein in Pfam
    PF13409 GST_N_2, 1 hit
    PIRSFiPIRSF015753 GST, 1 hit
    SUPFAMiSSF47616 SSF47616, 1 hit
    SSF52833 SSF52833, 2 hits
    PROSITEiView protein in PROSITE
    PS50405 GST_CTER, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q04806-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSEKSASNNK AEFKRQSSPF REIISADHPI YKPAKGRYWL YVALPCPWAQ
    60 70 80 90 100
    RTLITRALKG LAPIIGCSVA HWHLDDKGWR FLEEGDGKTN ERHWFDIAGG
    110 120 130 140 150
    ISSVNLNTST PVANIPNNAH RLLVDGTDEP HYGYKRLSDF YFKTKPDYKG
    160 170 180 190 200
    RFTVPVLWDL ETCTIVNNES SDIIGIMNSA AFDEFVGEEY RQVRLVPRSL
    210 220 230 240 250
    EAQITEFNSW VYDKINNGVY KAGFAECAEV YEREVTSLFQ YLDKLENLLD
    260 270 280 290 300
    KKYTDLEAEY GKNNKDKILD RYFAIGDTLT EADVRLYPTI VRFDVVYHQH
    310 320 330 340 350
    FKCNLATIRD DYSRIHTWLK NIYWRHEAFQ RTTDFTHIKL GYTRSQPRVN
    360
    PIGITPLGPK PDIRPP
    Length:366
    Mass (Da):42,403
    Last modified:November 1, 1997 - v1
    Checksum:iC0BF9F2D3AB5C15B
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z48639 Genomic DNA Translation: CAA88578.1
    AY692580 Genomic DNA Translation: AAT92599.1
    BK006946 Genomic DNA Translation: DAA10151.1
    PIRiS53073
    RefSeqiNP_013977.1, NM_001182757.1

    Genome annotation databases

    EnsemblFungiiYMR251W; YMR251W; YMR251W
    GeneIDi855292
    KEGGisce:YMR251W

    Similar proteinsi

    Entry informationi

    Entry nameiGTO3_YEAST
    AccessioniPrimary (citable) accession number: Q04806
    Secondary accession number(s): D6W077
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: March 28, 2018
    This is version 128 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health