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Protein

Glutathione S-transferase omega-like 3

Gene

GTO3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Active as '1-Cys' thiol transferase against beta-hydroxyethyl disulfide (HED), as dehydroascorbate reductase and as dimethylarsinic acid reductase, while not active against the standard GST substrate 1-chloro-2,4-dinitrobenzene (CDNB).1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.1 Publication

Kineticsi

  1. KM=1.40 mM for reduced glutathione (GSH)1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei46 – 461By similarity

GO - Molecular functioni

  1. glutathione transferase activity Source: SGD

GO - Biological processi

  1. glutathione metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciYEAST:G3O-32927-MONOMER.
SABIO-RKQ04806.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase omega-like 3 (EC:2.5.1.18)
Gene namesi
Name:GTO3
Ordered Locus Names:YMR251W
ORF Names:YM9920.05
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR251w.
SGDiS000004863. GTO3.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 366366Glutathione S-transferase omega-like 3PRO_0000203338Add
BLAST

Proteomic databases

PaxDbiQ04806.

Expressioni

Inductioni

Up-regulated by tert-butyl hydroperoxide (t-BOOH) in an MSN2/4-dependent manner.1 Publication

Gene expression databases

GenevestigatoriQ04806.

Interactioni

Protein-protein interaction databases

BioGridi35429. 4 interactions.
DIPiDIP-6643N.
IntActiQ04806. 1 interaction.
MINTiMINT-672625.
STRINGi4932.YMR251W.

Structurei

3D structure databases

ProteinModelPortaliQ04806.
SMRiQ04806. Positions 13-365.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini197 – 349153GST C-terminalAdd
BLAST

Sequence similaritiesi

Belongs to the GST superfamily. Omega family.Curated
Contains 1 GST C-terminal domain.Curated

Phylogenomic databases

eggNOGiCOG0435.
GeneTreeiENSGT00530000065151.
HOGENOMiHOG000245143.
InParanoidiQ04806.
OrthoDBiEOG722JK6.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 2 hits.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR016639. GST.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR32419. PTHR32419. 1 hit.
PfamiPF13409. GST_N_2. 1 hit.
[Graphical view]
PIRSFiPIRSF015753. GST. 1 hit.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 2 hits.
PROSITEiPS50405. GST_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04806-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSEKSASNNK AEFKRQSSPF REIISADHPI YKPAKGRYWL YVALPCPWAQ
60 70 80 90 100
RTLITRALKG LAPIIGCSVA HWHLDDKGWR FLEEGDGKTN ERHWFDIAGG
110 120 130 140 150
ISSVNLNTST PVANIPNNAH RLLVDGTDEP HYGYKRLSDF YFKTKPDYKG
160 170 180 190 200
RFTVPVLWDL ETCTIVNNES SDIIGIMNSA AFDEFVGEEY RQVRLVPRSL
210 220 230 240 250
EAQITEFNSW VYDKINNGVY KAGFAECAEV YEREVTSLFQ YLDKLENLLD
260 270 280 290 300
KKYTDLEAEY GKNNKDKILD RYFAIGDTLT EADVRLYPTI VRFDVVYHQH
310 320 330 340 350
FKCNLATIRD DYSRIHTWLK NIYWRHEAFQ RTTDFTHIKL GYTRSQPRVN
360
PIGITPLGPK PDIRPP
Length:366
Mass (Da):42,403
Last modified:November 1, 1997 - v1
Checksum:iC0BF9F2D3AB5C15B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48639 Genomic DNA. Translation: CAA88578.1.
AY692580 Genomic DNA. Translation: AAT92599.1.
BK006946 Genomic DNA. Translation: DAA10151.1.
PIRiS53073.
RefSeqiNP_013977.1. NM_001182757.1.

Genome annotation databases

EnsemblFungiiYMR251W; YMR251W; YMR251W.
GeneIDi855292.
KEGGisce:YMR251W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48639 Genomic DNA. Translation: CAA88578.1.
AY692580 Genomic DNA. Translation: AAT92599.1.
BK006946 Genomic DNA. Translation: DAA10151.1.
PIRiS53073.
RefSeqiNP_013977.1. NM_001182757.1.

3D structure databases

ProteinModelPortaliQ04806.
SMRiQ04806. Positions 13-365.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35429. 4 interactions.
DIPiDIP-6643N.
IntActiQ04806. 1 interaction.
MINTiMINT-672625.
STRINGi4932.YMR251W.

Proteomic databases

PaxDbiQ04806.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR251W; YMR251W; YMR251W.
GeneIDi855292.
KEGGisce:YMR251W.

Organism-specific databases

CYGDiYMR251w.
SGDiS000004863. GTO3.

Phylogenomic databases

eggNOGiCOG0435.
GeneTreeiENSGT00530000065151.
HOGENOMiHOG000245143.
InParanoidiQ04806.
OrthoDBiEOG722JK6.

Enzyme and pathway databases

BioCyciYEAST:G3O-32927-MONOMER.
SABIO-RKQ04806.

Miscellaneous databases

NextBioi978943.

Gene expression databases

GenevestigatoriQ04806.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 2 hits.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR016639. GST.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR32419. PTHR32419. 1 hit.
PfamiPF13409. GST_N_2. 1 hit.
[Graphical view]
PIRSFiPIRSF015753. GST. 1 hit.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 2 hits.
PROSITEiPS50405. GST_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Saccharomyces cerevisiae cells have three Omega class glutathione S-transferases acting as 1-Cys thiol transferases."
    Garcera A., Barreto L., Piedrafita L., Tamarit J., Herrero E.
    Biochem. J. 398:187-196(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
  5. "A peroxisomal glutathione transferase of Saccharomyces cerevisiae is functionally related to sulfur amino acid metabolism."
    Barreto L., Garcera A., Jansson K., Sunnerhagen P., Herrero E.
    Eukaryot. Cell 5:1748-1759(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INDUCTION.

Entry informationi

Entry nameiGTO3_YEAST
AccessioniPrimary (citable) accession number: Q04806
Secondary accession number(s): D6W077
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: January 7, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.