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Q04806

- GTO3_YEAST

UniProt

Q04806 - GTO3_YEAST

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Protein

Glutathione S-transferase omega-like 3

Gene
GTO3, YMR251W, YM9920.05
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Active as '1-Cys' thiol transferase against beta-hydroxyethyl disulfide (HED), as dehydroascorbate reductase and as dimethylarsinic acid reductase, while not active against the standard GST substrate 1-chloro-2,4-dinitrobenzene (CDNB).1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.1 Publication

Kineticsi

  1. KM=1.40 mM for reduced glutathione (GSH)1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei46 – 461 By similarity

GO - Molecular functioni

  1. glutathione transferase activity Source: SGD

GO - Biological processi

  1. glutathione metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciYEAST:G3O-32927-MONOMER.
SABIO-RKQ04806.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase omega-like 3 (EC:2.5.1.18)
Gene namesi
Name:GTO3
Ordered Locus Names:YMR251W
ORF Names:YM9920.05
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR251w.
SGDiS000004863. GTO3.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 366366Glutathione S-transferase omega-like 3PRO_0000203338Add
BLAST

Proteomic databases

PaxDbiQ04806.

Expressioni

Inductioni

Up-regulated by tert-butyl hydroperoxide (t-BOOH) in an MSN2/4-dependent manner.1 Publication

Gene expression databases

GenevestigatoriQ04806.

Interactioni

Protein-protein interaction databases

BioGridi35429. 4 interactions.
DIPiDIP-6643N.
IntActiQ04806. 1 interaction.
MINTiMINT-672625.
STRINGi4932.YMR251W.

Structurei

3D structure databases

ProteinModelPortaliQ04806.
SMRiQ04806. Positions 13-365.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini197 – 349153GST C-terminalAdd
BLAST

Sequence similaritiesi

Belongs to the GST superfamily. Omega family.

Phylogenomic databases

eggNOGiCOG0435.
GeneTreeiENSGT00530000065151.
HOGENOMiHOG000245143.
OMAiWISADNT.
OrthoDBiEOG722JK6.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 2 hits.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR016639. GST.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR32419. PTHR32419. 1 hit.
PfamiPF13409. GST_N_2. 1 hit.
[Graphical view]
PIRSFiPIRSF015753. GST. 1 hit.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 2 hits.
PROSITEiPS50405. GST_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04806-1 [UniParc]FASTAAdd to Basket

« Hide

MSEKSASNNK AEFKRQSSPF REIISADHPI YKPAKGRYWL YVALPCPWAQ    50
RTLITRALKG LAPIIGCSVA HWHLDDKGWR FLEEGDGKTN ERHWFDIAGG 100
ISSVNLNTST PVANIPNNAH RLLVDGTDEP HYGYKRLSDF YFKTKPDYKG 150
RFTVPVLWDL ETCTIVNNES SDIIGIMNSA AFDEFVGEEY RQVRLVPRSL 200
EAQITEFNSW VYDKINNGVY KAGFAECAEV YEREVTSLFQ YLDKLENLLD 250
KKYTDLEAEY GKNNKDKILD RYFAIGDTLT EADVRLYPTI VRFDVVYHQH 300
FKCNLATIRD DYSRIHTWLK NIYWRHEAFQ RTTDFTHIKL GYTRSQPRVN 350
PIGITPLGPK PDIRPP 366
Length:366
Mass (Da):42,403
Last modified:November 1, 1997 - v1
Checksum:iC0BF9F2D3AB5C15B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z48639 Genomic DNA. Translation: CAA88578.1.
AY692580 Genomic DNA. Translation: AAT92599.1.
BK006946 Genomic DNA. Translation: DAA10151.1.
PIRiS53073.
RefSeqiNP_013977.1. NM_001182757.1.

Genome annotation databases

EnsemblFungiiYMR251W; YMR251W; YMR251W.
GeneIDi855292.
KEGGisce:YMR251W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z48639 Genomic DNA. Translation: CAA88578.1 .
AY692580 Genomic DNA. Translation: AAT92599.1 .
BK006946 Genomic DNA. Translation: DAA10151.1 .
PIRi S53073.
RefSeqi NP_013977.1. NM_001182757.1.

3D structure databases

ProteinModelPortali Q04806.
SMRi Q04806. Positions 13-365.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35429. 4 interactions.
DIPi DIP-6643N.
IntActi Q04806. 1 interaction.
MINTi MINT-672625.
STRINGi 4932.YMR251W.

Proteomic databases

PaxDbi Q04806.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR251W ; YMR251W ; YMR251W .
GeneIDi 855292.
KEGGi sce:YMR251W.

Organism-specific databases

CYGDi YMR251w.
SGDi S000004863. GTO3.

Phylogenomic databases

eggNOGi COG0435.
GeneTreei ENSGT00530000065151.
HOGENOMi HOG000245143.
OMAi WISADNT.
OrthoDBi EOG722JK6.

Enzyme and pathway databases

BioCyci YEAST:G3O-32927-MONOMER.
SABIO-RK Q04806.

Miscellaneous databases

NextBioi 978943.

Gene expression databases

Genevestigatori Q04806.

Family and domain databases

Gene3Di 1.20.1050.10. 1 hit.
3.40.30.10. 2 hits.
InterProi IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR016639. GST.
IPR012336. Thioredoxin-like_fold.
[Graphical view ]
PANTHERi PTHR32419. PTHR32419. 1 hit.
Pfami PF13409. GST_N_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF015753. GST. 1 hit.
SUPFAMi SSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 2 hits.
PROSITEi PS50405. GST_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Saccharomyces cerevisiae cells have three Omega class glutathione S-transferases acting as 1-Cys thiol transferases."
    Garcera A., Barreto L., Piedrafita L., Tamarit J., Herrero E.
    Biochem. J. 398:187-196(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
  5. "A peroxisomal glutathione transferase of Saccharomyces cerevisiae is functionally related to sulfur amino acid metabolism."
    Barreto L., Garcera A., Jansson K., Sunnerhagen P., Herrero E.
    Eukaryot. Cell 5:1748-1759(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INDUCTION.

Entry informationi

Entry nameiGTO3_YEAST
AccessioniPrimary (citable) accession number: Q04806
Secondary accession number(s): D6W077
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 11, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

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