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Protein

Glutathione S-transferase omega-like 3

Gene

GTO3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Active as '1-Cys' thiol transferase against beta-hydroxyethyl disulfide (HED), as dehydroascorbate reductase and as dimethylarsinic acid reductase, while not active against the standard GST substrate 1-chloro-2,4-dinitrobenzene (CDNB).1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.1 Publication

Kineticsi

  1. KM=1.40 mM for reduced glutathione (GSH)1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei46 – 461By similarity

    GO - Molecular functioni

    • glutathione transferase activity Source: SGD

    GO - Biological processi

    • glutathione metabolic process Source: SGD
    Complete GO annotation...

    Keywords - Molecular functioni

    Transferase

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32927-MONOMER.
    SABIO-RKQ04806.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase omega-like 3 (EC:2.5.1.18)
    Gene namesi
    Name:GTO3
    Ordered Locus Names:YMR251W
    ORF Names:YM9920.05
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311 Componenti: Chromosome XIII

    Organism-specific databases

    CYGDiYMR251w.
    EuPathDBiFungiDB:YMR251W.
    SGDiS000004863. GTO3.

    Subcellular locationi

    GO - Cellular componenti

    • cytoplasm Source: SGD
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 366366Glutathione S-transferase omega-like 3PRO_0000203338Add
    BLAST

    Proteomic databases

    PaxDbiQ04806.

    Expressioni

    Inductioni

    Up-regulated by tert-butyl hydroperoxide (t-BOOH) in an MSN2/4-dependent manner.1 Publication

    Gene expression databases

    GenevestigatoriQ04806.

    Interactioni

    Protein-protein interaction databases

    BioGridi35429. 4 interactions.
    DIPiDIP-6643N.
    IntActiQ04806. 1 interaction.
    MINTiMINT-672625.
    STRINGi4932.YMR251W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ04806.
    SMRiQ04806. Positions 13-365.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini197 – 349153GST C-terminalAdd
    BLAST

    Sequence similaritiesi

    Belongs to the GST superfamily. Omega family.Curated
    Contains 1 GST C-terminal domain.Curated

    Phylogenomic databases

    eggNOGiCOG0435.
    GeneTreeiENSGT00530000065151.
    HOGENOMiHOG000245143.
    InParanoidiQ04806.
    OrthoDBiEOG722JK6.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 2 hits.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR016639. GST.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PANTHERiPTHR32419. PTHR32419. 1 hit.
    PfamiPF13409. GST_N_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF015753. GST. 1 hit.
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 2 hits.
    PROSITEiPS50405. GST_CTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q04806-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSEKSASNNK AEFKRQSSPF REIISADHPI YKPAKGRYWL YVALPCPWAQ
    60 70 80 90 100
    RTLITRALKG LAPIIGCSVA HWHLDDKGWR FLEEGDGKTN ERHWFDIAGG
    110 120 130 140 150
    ISSVNLNTST PVANIPNNAH RLLVDGTDEP HYGYKRLSDF YFKTKPDYKG
    160 170 180 190 200
    RFTVPVLWDL ETCTIVNNES SDIIGIMNSA AFDEFVGEEY RQVRLVPRSL
    210 220 230 240 250
    EAQITEFNSW VYDKINNGVY KAGFAECAEV YEREVTSLFQ YLDKLENLLD
    260 270 280 290 300
    KKYTDLEAEY GKNNKDKILD RYFAIGDTLT EADVRLYPTI VRFDVVYHQH
    310 320 330 340 350
    FKCNLATIRD DYSRIHTWLK NIYWRHEAFQ RTTDFTHIKL GYTRSQPRVN
    360
    PIGITPLGPK PDIRPP
    Length:366
    Mass (Da):42,403
    Last modified:November 1, 1997 - v1
    Checksum:iC0BF9F2D3AB5C15B
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z48639 Genomic DNA. Translation: CAA88578.1.
    AY692580 Genomic DNA. Translation: AAT92599.1.
    BK006946 Genomic DNA. Translation: DAA10151.1.
    PIRiS53073.
    RefSeqiNP_013977.1. NM_001182757.1.

    Genome annotation databases

    EnsemblFungiiYMR251W; YMR251W; YMR251W.
    GeneIDi855292.
    KEGGisce:YMR251W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z48639 Genomic DNA. Translation: CAA88578.1.
    AY692580 Genomic DNA. Translation: AAT92599.1.
    BK006946 Genomic DNA. Translation: DAA10151.1.
    PIRiS53073.
    RefSeqiNP_013977.1. NM_001182757.1.

    3D structure databases

    ProteinModelPortaliQ04806.
    SMRiQ04806. Positions 13-365.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi35429. 4 interactions.
    DIPiDIP-6643N.
    IntActiQ04806. 1 interaction.
    MINTiMINT-672625.
    STRINGi4932.YMR251W.

    Proteomic databases

    PaxDbiQ04806.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYMR251W; YMR251W; YMR251W.
    GeneIDi855292.
    KEGGisce:YMR251W.

    Organism-specific databases

    CYGDiYMR251w.
    EuPathDBiFungiDB:YMR251W.
    SGDiS000004863. GTO3.

    Phylogenomic databases

    eggNOGiCOG0435.
    GeneTreeiENSGT00530000065151.
    HOGENOMiHOG000245143.
    InParanoidiQ04806.
    OrthoDBiEOG722JK6.

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32927-MONOMER.
    SABIO-RKQ04806.

    Miscellaneous databases

    NextBioi978943.
    PROiQ04806.

    Gene expression databases

    GenevestigatoriQ04806.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 2 hits.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR016639. GST.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PANTHERiPTHR32419. PTHR32419. 1 hit.
    PfamiPF13409. GST_N_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF015753. GST. 1 hit.
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 2 hits.
    PROSITEiPS50405. GST_CTER. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Saccharomyces cerevisiae cells have three Omega class glutathione S-transferases acting as 1-Cys thiol transferases."
      Garcera A., Barreto L., Piedrafita L., Tamarit J., Herrero E.
      Biochem. J. 398:187-196(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
    5. "A peroxisomal glutathione transferase of Saccharomyces cerevisiae is functionally related to sulfur amino acid metabolism."
      Barreto L., Garcera A., Jansson K., Sunnerhagen P., Herrero E.
      Eukaryot. Cell 5:1748-1759(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INDUCTION.

    Entry informationi

    Entry nameiGTO3_YEAST
    AccessioniPrimary (citable) accession number: Q04806
    Secondary accession number(s): D6W077
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: April 29, 2015
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.