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Q04806 (GTO3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione S-transferase omega-like 3
EC=2.5.1.18
Gene names
Name:GTO3
Ordered Locus Names:YMR251W
ORF Names:YM9920.05
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length366 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Active as '1-Cys' thiol transferase against beta-hydroxyethyl disulfide (HED), as dehydroascorbate reductase and as dimethylarsinic acid reductase, while not active against the standard GST substrate 1-chloro-2,4-dinitrobenzene (CDNB). Ref.4

Catalytic activity

RX + glutathione = HX + R-S-glutathione. Ref.4

Subcellular location

Cytoplasm Ref.5.

Induction

Up-regulated by tert-butyl hydroperoxide (t-BOOH) in an MSN2/4-dependent manner. Ref.5

Sequence similarities

Belongs to the GST superfamily. Omega family.

Contains 1 GST C-terminal domain.

Biophysicochemical properties

Kinetic parameters:

KM=1.40 mM for reduced glutathione (GSH) Ref.4

Ontologies

Keywords
   Cellular componentCytoplasm
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutathione metabolic process

Inferred from sequence or structural similarity Ref.4. Source: SGD

   Cellular_componentcytoplasm

Inferred from direct assay Ref.5. Source: SGD

   Molecular_functionglutathione transferase activity

Inferred from direct assay Ref.4. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 366366Glutathione S-transferase omega-like 3
PRO_0000203338

Regions

Domain197 – 349153GST C-terminal

Sites

Active site461 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q04806 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: C0BF9F2D3AB5C15B

FASTA36642,403
        10         20         30         40         50         60 
MSEKSASNNK AEFKRQSSPF REIISADHPI YKPAKGRYWL YVALPCPWAQ RTLITRALKG 

        70         80         90        100        110        120 
LAPIIGCSVA HWHLDDKGWR FLEEGDGKTN ERHWFDIAGG ISSVNLNTST PVANIPNNAH 

       130        140        150        160        170        180 
RLLVDGTDEP HYGYKRLSDF YFKTKPDYKG RFTVPVLWDL ETCTIVNNES SDIIGIMNSA 

       190        200        210        220        230        240 
AFDEFVGEEY RQVRLVPRSL EAQITEFNSW VYDKINNGVY KAGFAECAEV YEREVTSLFQ 

       250        260        270        280        290        300 
YLDKLENLLD KKYTDLEAEY GKNNKDKILD RYFAIGDTLT EADVRLYPTI VRFDVVYHQH 

       310        320        330        340        350        360 
FKCNLATIRD DYSRIHTWLK NIYWRHEAFQ RTTDFTHIKL GYTRSQPRVN PIGITPLGPK 


PDIRPP

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Saccharomyces cerevisiae cells have three Omega class glutathione S-transferases acting as 1-Cys thiol transferases."
Garcera A., Barreto L., Piedrafita L., Tamarit J., Herrero E.
Biochem. J. 398:187-196(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
[5]"A peroxisomal glutathione transferase of Saccharomyces cerevisiae is functionally related to sulfur amino acid metabolism."
Barreto L., Garcera A., Jansson K., Sunnerhagen P., Herrero E.
Eukaryot. Cell 5:1748-1759(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z48639 Genomic DNA. Translation: CAA88578.1.
AY692580 Genomic DNA. Translation: AAT92599.1.
BK006946 Genomic DNA. Translation: DAA10151.1.
PIRS53073.
RefSeqNP_013977.1. NM_001182757.1.

3D structure databases

ProteinModelPortalQ04806.
SMRQ04806. Positions 13-365.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-6643N.
IntActQ04806. 1 interaction.
MINTMINT-672625.
STRING4932.YMR251W.

Proteomic databases

PaxDbQ04806.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR251W; YMR251W; YMR251W.
GeneID855292.
KEGGsce:YMR251W.

Organism-specific databases

CYGDYMR251w.
SGDS000004863. GTO3.

Phylogenomic databases

eggNOGCOG0435.
GeneTreeENSGT00530000065151.
HOGENOMHOG000245143.
OMACPWAQRT.
OrthoDBEOG4617CM.

Enzyme and pathway databases

SABIO-RKQ04806.

Gene expression databases

GenevestigatorQ04806.
GermOnlineYMR251W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D1.20.1050.10. 1 hit.
3.40.30.10. 2 hits.
InterProIPR010987. Glutathione-S-Trfase_C-like.
IPR017933. Glutathione_S_Trfase/Cl_chnl_C.
IPR016639. GST.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERPTHR32419. PTHR32419. 1 hit.
PIRSFPIRSF015753. GST. 1 hit.
SUPFAMSSF47616. GST_C_like. 1 hit.
SSF52833. Thiordxn-like_fd. 1 hit.
PROSITEPS50405. GST_CTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio978943.

Entry information

Entry nameGTO3_YEAST
AccessionPrimary (citable) accession number: Q04806
Secondary accession number(s): D6W077
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 1, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

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