Reviewed, UniProtKB/Swiss-Prot Q04795 (AK1_BACSU)

Names and origin

Protein names

Recommended name:
    Aspartokinase 1
    EC=2.7.2.4
Alternative name(s):
    Aspartokinase I
    Aspartate kinase 1

Gene names

Name:	dapG
Synonyms:	lssD
Ordered Locus Names:	BSU16760

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate = ADP + 4-phospho-L-aspartate.
Enzyme regulation	Diaminopimelate-sensitive.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; tetrahydrodipicolinate from L-aspartate: step 1/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.
Subunit structure	Tetramer consisting of 2 isoforms Alpha (catalytic) and 2 isoforms Beta (function not known) By similarity.
Sequence similarities	Belongs to the aspartokinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Coding sequence diversity	Alternative initiation
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	aspartate kinase activity Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform Alpha (identifier: Q04795-1)

Also known as: Aspartokinase 1 subunit alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Beta (identifier: Q04795-2)

Also known as: Aspartokinase 1 subunit beta;

The sequence of this isoform differs from the canonical sequence as follows:
     1-244: Missing.
     245-245: V → M

Notes: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 404	404	Aspartokinase 1		PRO_0000002367
Natural variations
	Alternative sequence	1 – 244	244	Missing in isoform Beta.		VSP_018652
	Alternative sequence	245	1	V → M in isoform Beta.		VSP_018985
Experimental info
	Sequence conflict	399	1	E → V in AAA22384. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform Alpha (Aspartokinase 1 subunit alpha) [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 49A6DA70D70047C4	FASTA	404	42,978
	10 20 30 40 50 60 MKIIVQKFGG TSVKDDKGRK LALGHIKEAI SEGYKVVVVV SAMGRKGDPY ATDSLLGLLY 70 80 90 100 110 120 GDQSAISPRE QDLLLSCGET ISSVVFTSML LDNGVKAAAL TGAQAGFLTN DQHTNAKIIE 130 140 150 160 170 180 MKPERLFSVL ANHDAVVVAG FQGATEKGDT TTIGRGGSDT SAAALGAAVD AEYIDIFTDV 190 200 210 220 230 240 EGVMTADPRV VENAKPLPVV TYTEICNLAY QGAKVISPRA VEIAMQAKVP IRVRSTYSND 250 260 270 280 290 300 KGTLVTSHHS SKVGSDVFER LITGIAHVKD VTQFKVPAKI GQYNVQTEVF KAMANAGISV 310 320 330 340 350 360 DFFNITPSEI VYTVAGNKTE TAQRILMDMG YDPMVTRNCA KVSAVGAGIM GVPGVTSKIV 370 380 390 400 SALSEKEIPI LQSADSHTTI WVLVHEADMV PAVNALHEEF ELSK
	Isoform Beta (Aspartokinase 1 subunit beta) [UniParc]. Checksum: 80438B01A3CAB6FC		160	17,291
	10 20 30 40 50 60 MTSHHSSKVG SDVFERLITG IAHVKDVTQF KVPAKIGQYN VQTEVFKAMA NAGISVDFFN 70 80 90 100 110 120 ITPSEIVYTV AGNKTETAQR ILMDMGYDPM VTRNCAKVSA VGAGIMGVPG VTSKIVSALS 130 140 150 160 EKEIPILQSA DSHTTIWVLV HEADMVPAVN ALHEEFELSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Organization and nucleotide sequence of the Bacillus subtilis diaminopimelate operon, a cluster of genes encoding the first three enzymes of diaminopimelate synthesis and dipicolinate synthase." Chen N.-Y., Jiang S.-Q., Klein D.A., Paulus H. J. Biol. Chem. 268:9448-9465(1993) [PubMed: 8098035] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L08471 Genomic DNA. Translation: AAA22384.1. Z99112 Genomic DNA. Translation: CAB13549.1.
PIR	C46665.
RefSeq	NP_389558.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	936710.
GenomeReviews	Gene locus BSU16760 in contig AL009126_GR.
KEGG	bsu:BSU16760.
NMPDR	fig|224308.1.peg.1679.
Organism-specific databases
SubtiList	BG10784. dapG. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	Q04795.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU1677-MON.
Family and domain databases
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR005260. Asp_kin_monofn. IPR001341. Asp_kin_reg. IPR001057. Glu_5kinase. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PRINTS	PR00474. GLU5KINASE.
TIGRFAMs	TIGR00656. asp_kin_monofn. 1 hit. TIGR00657. asp_kinases. 1 hit.
PROSITE	PS00324. ASPARTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AK1_BACSU

Accession

Primary (citable) accession number: Q04795
Secondary accession number(s): O31759

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	May 30, 2000
Last modified:	November 25, 2008
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)