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Protein

Glutamate decarboxylase

Gene

GAD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-glutamate = 4-aminobutanoate + CO2.

Cofactori

GO - Molecular functioni

  1. glutamate decarboxylase activity Source: SGD
  2. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. cellular response to oxidative stress Source: SGD
  2. glutamate catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciYEAST:YMR250W-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate decarboxylase (EC:4.1.1.15)
Short name:
GAD
Gene namesi
Name:GAD1
Ordered Locus Names:YMR250W
ORF Names:YM9920.04
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR250w.
SGDiS000004862. GAD1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 585585Glutamate decarboxylasePRO_0000146977Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei318 – 3181N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

MaxQBiQ04792.
PeptideAtlasiQ04792.

Expressioni

Gene expression databases

ExpressionAtlasiQ04792. differential.
GenevestigatoriQ04792.

Interactioni

Protein-protein interaction databases

BioGridi35428. 26 interactions.
DIPiDIP-2552N.
IntActiQ04792. 3 interactions.
MINTiMINT-423058.
STRINGi4932.YMR250W.

Structurei

3D structure databases

ProteinModelPortaliQ04792.
SMRiQ04792. Positions 59-521.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the group II decarboxylase family.Curated

Phylogenomic databases

HOGENOMiHOG000070228.
InParanoidiQ04792.
KOiK01580.
OMAiHHIELAS.
OrthoDBiEOG7P2Z22.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
InterProiIPR010107. Glutamate_decarboxylase.
IPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERiPTHR11999:SF1. PTHR11999:SF1. 1 hit.
PfamiPF00282. Pyridoxal_deC. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01788. Glu-decarb-GAD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q04792-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLHRHGSKQK NFENIAGKVV HDLAGLQLLS NDVQKSAVQS GHQGSNNMRD
60 70 80 90 100
TSSQGMANKY SVPKKGLPAD LSYQLIHNEL TLDGNPHLNL ASFVNTFTTD
110 120 130 140 150
QARKLIDENL TKNLADNDEY PQLIELTQRC ISMLAQLWHA NPDEEPIGCA
160 170 180 190 200
TTGSSEAIML GGLAMKKRWE HRMKNAGKDA SKPNIIMSSA CQVALEKFTR
210 220 230 240 250
YFEVECRLVP VSHRSHHMLD PESLWDYVDE NTIGCFVILG TTYTGHLENV
260 270 280 290 300
EKVADVLSQI EAKHPDWSNT DIPIHADGAS GGFIIPFGFE KEHMKAYGME
310 320 330 340 350
RWGFNHPRVV SMNTSGHKFG LTTPGLGWVL WRDESLLADE LRFKLKYLGG
360 370 380 390 400
VEETFGLNFS RPGFQVVHQY FNFVSLGHSG YRTQFQNSLF VARAFSFELL
410 420 430 440 450
NSSKLPGCFE IVSSIHESIE NDSAPKSVKD YWEHPQAYKP GVPLVAFKLS
460 470 480 490 500
KKFHEEYPEV PQAILSSLLR GRGWIIPNYP LPKATDGSDE KEVLRVVFRS
510 520 530 540 550
EMKLDLAQLL IVDIESILTK LIHSYEKVCH HIELASEQTP ERKSSFIYEM
560 570 580
LLALASPQDD IPTPDEIEKK NKLKETTTRN YRGTC
Length:585
Mass (Da):65,990
Last modified:November 1, 1997 - v1
Checksum:iDDD22868CEB7B955
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48639 Genomic DNA. Translation: CAA88577.1.
BK006946 Genomic DNA. Translation: DAA10150.1.
PIRiS53072.
RefSeqiNP_013976.1. NM_001182756.1.

Genome annotation databases

EnsemblFungiiYMR250W; YMR250W; YMR250W.
GeneIDi855291.
KEGGisce:YMR250W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48639 Genomic DNA. Translation: CAA88577.1.
BK006946 Genomic DNA. Translation: DAA10150.1.
PIRiS53072.
RefSeqiNP_013976.1. NM_001182756.1.

3D structure databases

ProteinModelPortaliQ04792.
SMRiQ04792. Positions 59-521.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35428. 26 interactions.
DIPiDIP-2552N.
IntActiQ04792. 3 interactions.
MINTiMINT-423058.
STRINGi4932.YMR250W.

Proteomic databases

MaxQBiQ04792.
PeptideAtlasiQ04792.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR250W; YMR250W; YMR250W.
GeneIDi855291.
KEGGisce:YMR250W.

Organism-specific databases

CYGDiYMR250w.
SGDiS000004862. GAD1.

Phylogenomic databases

HOGENOMiHOG000070228.
InParanoidiQ04792.
KOiK01580.
OMAiHHIELAS.
OrthoDBiEOG7P2Z22.

Enzyme and pathway databases

BioCyciYEAST:YMR250W-MONOMER.

Miscellaneous databases

NextBioi978940.

Gene expression databases

ExpressionAtlasiQ04792. differential.
GenevestigatoriQ04792.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
InterProiIPR010107. Glutamate_decarboxylase.
IPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERiPTHR11999:SF1. PTHR11999:SF1. 1 hit.
PfamiPF00282. Pyridoxal_deC. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01788. Glu-decarb-GAD. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDCE_YEAST
AccessioniPrimary (citable) accession number: Q04792
Secondary accession number(s): D6W076
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: February 4, 2015
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1200 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.