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Protein

Acetolactate synthase

Gene

alsS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

2 pyruvate = 2-acetolactate + CO2.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity
  • thiamine diphosphateBy similarityNote: Binds 1 thiamine pyrophosphate per subunit.By similarity

Pathwayi: (R,R)-butane-2,3-diol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes (R,R)-butane-2,3-diol from pyruvate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Acetolactate synthase (alsS)
  2. Alpha-acetolactate decarboxylase (alsD), Alpha-acetolactate decarboxylase (alsD)
  3. no protein annotated in this organism
This subpathway is part of the pathway (R,R)-butane-2,3-diol biosynthesis, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R,R)-butane-2,3-diol from pyruvate, the pathway (R,R)-butane-2,3-diol biosynthesis and in Polyol metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei60Thiamine pyrophosphateBy similarity1
Binding sitei162FADBy similarity1
Metal bindingi450MagnesiumBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi266 – 287FADBy similarityAdd BLAST22
Nucleotide bindingi308 – 327FADBy similarityAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processAcetoin biosynthesis
LigandFAD, Flavoprotein, Magnesium, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciBSUB:BSU36010-MONOMER
UniPathwayiUPA00626; UER00677

Names & Taxonomyi

Protein namesi
Recommended name:
Acetolactate synthase (EC:2.2.1.6)
Alternative name(s):
ALS
Acetohydroxy-acid synthase
Gene namesi
Name:alsS
Ordered Locus Names:BSU36010
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000907991 – 570Acetolactate synthaseAdd BLAST570

Proteomic databases

PaxDbiQ04789

Expressioni

Inductioni

Strongly induced under anaerobic conditions. Activated by ResDE, Fnr and ArfM.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100019476

Structurei

3D structure databases

ProteinModelPortaliQ04789
SMRiQ04789
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni399 – 479Thiamine pyrophosphate bindingAdd BLAST81

Sequence similaritiesi

Belongs to the TPP enzyme family.Curated

Phylogenomic databases

eggNOGiCOG0028 LUCA
HOGENOMiHOG000258447
InParanoidiQ04789
KOiK01652
OMAiIRWKQAV
PhylomeDBiQ04789

Family and domain databases

InterProiView protein in InterPro
IPR012782 Acetolactate_synth_catblc
IPR029035 DHS-like_NAD/FAD-binding_dom
IPR029061 THDP-binding
IPR012000 Thiamin_PyroP_enz_cen_dom
IPR012001 Thiamin_PyroP_enz_TPP-bd_dom
IPR000399 TPP-bd_CS
IPR011766 TPP_enzyme-bd_C
PfamiView protein in Pfam
PF02775 TPP_enzyme_C, 1 hit
PF00205 TPP_enzyme_M, 1 hit
PF02776 TPP_enzyme_N, 1 hit
SUPFAMiSSF52467 SSF52467, 1 hit
SSF52518 SSF52518, 2 hits
TIGRFAMsiTIGR02418 acolac_catab, 1 hit
PROSITEiView protein in PROSITE
PS00187 TPP_ENZYMES, 1 hit

Sequencei

Sequence statusi: Complete.

Q04789-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKATKEQKS LVKNRGAELV VDCLVEQGVT HVFGIPGAKI DAVFDALQDK
60 70 80 90 100
GPEIIVARHE QNAAFMAQAV GRLTGKPGVV LVTSGPGASN LATGLLTANT
110 120 130 140 150
EGDPVVALAG NVIRADRLKR THQSLDNAAL FQPITKYSVE VQDVKNIPEA
160 170 180 190 200
VTNAFRIASA GQAGAAFVSF PQDVVNEVTN TKNVRAVAAP KLGPAADDAI
210 220 230 240 250
SAAIAKIQTA KLPVVLVGMK GGRPEAIKAV RKLLKKVQLP FVETYQAAGT
260 270 280 290 300
LSRDLEDQYF GRIGLFRNQP GDLLLEQADV VLTIGYDPIE YDPKFWNING
310 320 330 340 350
DRTIIHLDEI IADIDHAYQP DLELIGDIPS TINHIEHDAV KVEFAEREQK
360 370 380 390 400
ILSDLKQYMH EGEQVPADWK SDRAHPLEIV KELRNAVDDH VTVTCDIGSH
410 420 430 440 450
AIWMSRYFRS YEPLTLMISN GMQTLGVALP WAIGASLVKP GEKVVSVSGD
460 470 480 490 500
GGFLFSAMEL ETAVRLKAPI VHIVWNDSTY DMVAFQQLKK YNRTSAVDFG
510 520 530 540 550
NIDIVKYAES FGATGLRVES PDQLADVLRQ GMNAEGPVII DVPVDYSDNI
560 570
NLASDKLPKE FGELMKTKAL
Length:570
Mass (Da):62,004
Last modified:June 16, 2009 - v3
Checksum:i47B7A64CE6C78EF7
GO

Sequence cautioni

The sequence AAA22222 differs from that shown. Reason: Frameshift at position 64.Curated
The sequence CAB07802 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti116D → Y in AAA22222 (PubMed:7685336).Curated1
Sequence conflicti401A → S in AAA22222 (PubMed:7685336).Curated1
Sequence conflicti484A → H in AAA22222 (PubMed:7685336).Curated1
Sequence conflicti515G → A in AAA22222 (PubMed:7685336).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04470 Genomic DNA Translation: AAA22222.1 Frameshift.
Z93767 Genomic DNA Translation: CAB07802.1 Different initiation.
AL009126 Genomic DNA Translation: CAB15618.2
PIRiH69584
RefSeqiNP_391482.2, NC_000964.3
WP_003244057.1, NZ_JNCM01000034.1

Genome annotation databases

EnsemblBacteriaiCAB15618; CAB15618; BSU36010
GeneIDi936852
KEGGibsu:BSU36010
PATRICifig|224308.179.peg.3898

Similar proteinsi

Entry informationi

Entry nameiILVX_BACSU
AccessioniPrimary (citable) accession number: Q04789
Secondary accession number(s): O05225
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: June 16, 2009
Last modified: March 28, 2018
This is version 129 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health