Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q04788 (PIT1_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pituitary-specific positive transcription factor 1

Short name=PIT-1
Alternative name(s):
Growth hormone factor 1
Short name=GHF-1
Gene names
Name:POU1F1
Synonyms:PIT-1, PIT1
OrganismSus scrofa (Pig) [Reference proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus

Protein attributes

Sequence length291 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'.

Subcellular location

Nucleus.

Sequence similarities

Belongs to the POU transcription factor family. Class-1 subfamily.

Contains 1 homeobox DNA-binding domain.

Contains 1 POU-specific domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainHomeobox
   LigandDNA-binding
   Molecular functionActivator
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtranscription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionsequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 291291Pituitary-specific positive transcription factor 1
PRO_0000100700

Regions

Domain124 – 19875POU-specific
DNA binding214 – 27360Homeobox

Experimental info

Sequence conflict641P → S in AAB65789. Ref.2
Sequence conflict941Y → H in AAB65789. Ref.2
Sequence conflict1361P → A in AAB65789. Ref.2
Sequence conflict1411V → A in CAA73939. Ref.1
Sequence conflict1441I → M in AAA31108. Ref.3
Sequence conflict155 – 1617EALAAVH → QVAAAWT in CAA73939. Ref.1
Sequence conflict1831A → R in CAA73939. Ref.1
Sequence conflict1961E → V in CAA73939. Ref.1
Sequence conflict2591R → S in AAA31108. Ref.3
Sequence conflict2601V → F in CAA73939. Ref.1
Sequence conflict2651R → H Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q04788 [UniParc].

Last modified April 27, 2001. Version 3.
Checksum: C13EB6634697F8EA

FASTA29133,033
        10         20         30         40         50         60 
MSCQPFTSAD TFIPLNSESS ATLPLIMHHS AAECLPASNH ATNVMSTATG LHYSVPSCHY 

        70         80         90        100        110        120 
GNQPSTYGVM AGSLTPCLYK FPDHTLSHGF PPMYQPLLPE DPTAADFKQE LRRKSKLVEE 

       130        140        150        160        170        180 
PIDMDSPEIR ELEKFPNEFK VRRIKLGYTQ TNVGEALAAV HGSEFSQTTI CRFENLQLSF 

       190        200        210        220        230        240 
KNACKLKAIL SKWLEEAEQV GALYNEKVGA NERKRKRRTT ISIAAKDALE RHFGEQNKPS 

       250        260        270        280        290 
SQEILRMAEE LNLEKEVVRV WFCNRRQREK RVKTSLNQSL FTISKEHLEC R 

« Hide

References

[1]"Molecular cloning of pig (Sus scrofa) pituitary transcription factor GHF-1/Pit-1."
Vila V., Sanchez M.P., Malagon M.M., Gracia F., Castrillo J.-L.
Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Pituitary.
[2]"Cloning of the full length pig PIT-1 cDNA and its alternative transcripts, and functional studies of their encoded proteins."
Yu T.-P., Sun H.S., Rothschild M.F., Tuggle C.K.
Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Cloning and restriction fragment length polymorphism analysis of a cDNA for swine PIT-1, a gene controlling growth hormone expression."
Tuggle C.K., Yu T.-P., Helm J., Rothschild M.F.
Anim. Genet. 24:17-21(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 139-267.
Strain: Duroc.
Tissue: Brain cortex and Pituitary.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y13617 mRNA. Translation: CAA73939.1.
AF016251 mRNA. Translation: AAB65789.2.
L03841 mRNA. Translation: AAA31108.1.
RefSeqNP_999328.1. NM_214163.1.
UniGeneSsc.16019.

3D structure databases

ProteinModelPortalQ04788.
SMRQ04788. Positions 129-273.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID397325.
KEGGssc:397325.

Organism-specific databases

CTD5449.

Phylogenomic databases

eggNOGNOG241031.
HOGENOMHOG000116302.
HOVERGENHBG053120.
KOK09363.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR015586. Pit_1.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view]
PANTHERPTHR11636:SF17. PTHR11636:SF17. 1 hit.
PfamPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSPR00028. POUDOMAIN.
SMARTSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePIT1_PIG
AccessionPrimary (citable) accession number: Q04788
Secondary accession number(s): O18807, O46410
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: April 27, 2001
Last modified: February 19, 2014
This is version 102 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families