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Protein

Pituitary-specific positive transcription factor 1

Gene

POU1F1

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi214 – 27360HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. sequence-specific DNA binding Source: InterPro
  2. sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  1. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pituitary-specific positive transcription factor 1
Short name:
PIT-1
Alternative name(s):
Growth hormone factor 1
Short name:
GHF-1
Gene namesi
Name:POU1F1
Synonyms:PIT-1, PIT1
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 291291Pituitary-specific positive transcription factor 1PRO_0000100700Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ04788.
SMRiQ04788. Positions 129-273.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini124 – 19875POU-specificPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG241031.
HOGENOMiHOG000116302.
HOVERGENiHBG053120.
InParanoidiQ04788.
KOiK09363.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR015586. Pit_1.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view]
PANTHERiPTHR11636:SF82. PTHR11636:SF82. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04788-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSCQPFTSAD TFIPLNSESS ATLPLIMHHS AAECLPASNH ATNVMSTATG
60 70 80 90 100
LHYSVPSCHY GNQPSTYGVM AGSLTPCLYK FPDHTLSHGF PPMYQPLLPE
110 120 130 140 150
DPTAADFKQE LRRKSKLVEE PIDMDSPEIR ELEKFPNEFK VRRIKLGYTQ
160 170 180 190 200
TNVGEALAAV HGSEFSQTTI CRFENLQLSF KNACKLKAIL SKWLEEAEQV
210 220 230 240 250
GALYNEKVGA NERKRKRRTT ISIAAKDALE RHFGEQNKPS SQEILRMAEE
260 270 280 290
LNLEKEVVRV WFCNRRQREK RVKTSLNQSL FTISKEHLEC R
Length:291
Mass (Da):33,033
Last modified:April 26, 2001 - v3
Checksum:iC13EB6634697F8EA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti64 – 641P → S in AAB65789 (Ref. 2) Curated
Sequence conflicti94 – 941Y → H in AAB65789 (Ref. 2) Curated
Sequence conflicti136 – 1361P → A in AAB65789 (Ref. 2) Curated
Sequence conflicti141 – 1411V → A in CAA73939 (Ref. 1) Curated
Sequence conflicti144 – 1441I → M in AAA31108 (PubMed:8098921).Curated
Sequence conflicti155 – 1617EALAAVH → QVAAAWT in CAA73939 (Ref. 1) Curated
Sequence conflicti183 – 1831A → R in CAA73939 (Ref. 1) Curated
Sequence conflicti196 – 1961E → V in CAA73939 (Ref. 1) Curated
Sequence conflicti259 – 2591R → S in AAA31108 (PubMed:8098921).Curated
Sequence conflicti260 – 2601V → F in CAA73939 (Ref. 1) Curated
Sequence conflicti265 – 2651R → H (PubMed:8098921).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13617 mRNA. Translation: CAA73939.1.
AF016251 mRNA. Translation: AAB65789.2.
L03841 mRNA. Translation: AAA31108.1.
RefSeqiNP_999328.1. NM_214163.1.
UniGeneiSsc.16019.

Genome annotation databases

GeneIDi397325.
KEGGissc:397325.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13617 mRNA. Translation: CAA73939.1.
AF016251 mRNA. Translation: AAB65789.2.
L03841 mRNA. Translation: AAA31108.1.
RefSeqiNP_999328.1. NM_214163.1.
UniGeneiSsc.16019.

3D structure databases

ProteinModelPortaliQ04788.
SMRiQ04788. Positions 129-273.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi397325.
KEGGissc:397325.

Organism-specific databases

CTDi5449.

Phylogenomic databases

eggNOGiNOG241031.
HOGENOMiHOG000116302.
HOVERGENiHBG053120.
InParanoidiQ04788.
KOiK09363.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR015586. Pit_1.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view]
PANTHERiPTHR11636:SF82. PTHR11636:SF82. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of pig (Sus scrofa) pituitary transcription factor GHF-1/Pit-1."
    Vila V., Sanchez M.P., Malagon M.M., Gracia F., Castrillo J.-L.
    Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pituitary.
  2. "Cloning of the full length pig PIT-1 cDNA and its alternative transcripts, and functional studies of their encoded proteins."
    Yu T.-P., Sun H.S., Rothschild M.F., Tuggle C.K.
    Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Cloning and restriction fragment length polymorphism analysis of a cDNA for swine PIT-1, a gene controlling growth hormone expression."
    Tuggle C.K., Yu T.-P., Helm J., Rothschild M.F.
    Anim. Genet. 24:17-21(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 139-267.
    Strain: Duroc.
    Tissue: Brain cortex and Pituitary.

Entry informationi

Entry nameiPIT1_PIG
AccessioniPrimary (citable) accession number: Q04788
Secondary accession number(s): O18807, O46410
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 31, 1994
Last sequence update: April 26, 2001
Last modified: January 6, 2015
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.