Reviewed, UniProtKB/Swiss-Prot Q04786 (HEX_VIBVU)

Names and origin

Protein names

Recommended name:
    Beta-hexosaminidase
    EC=3.2.1.52
Alternative name(s):
    N-acetyl-beta-glucosaminidase
    Beta-N-acetylhexosaminidase
    Chitobiase

Gene names

Name:

hex

Organism

Vibrio vulnificus

Taxonomic identifier

672 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	847 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Hydrolysis of terminal, non-reducing N-acetyl-beta-D-glucosamine residues in chitobiose and higher analogs, and in glycoproteins.
Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.
Pathway	Glycan degradation; chitin degradation.
Sequence similarities	Belongs to the glycosyl hydrolase 20 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Polysaccharide degradation
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-N-acetylhexosaminidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro polysaccharide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 847	847	Beta-hexosaminidase		PRO_0000215378
Sites
	Active site	519	1	Proton donor By similarity
Amino acid modifications
	Disulfide bond	31 ↔ 40		By similarity
	Disulfide bond	377 ↔ 385		By similarity
	Disulfide bond	484 ↔ 530		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q04786-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 3C4405EFA34F3D14	FASTA	847	94,277
	10 20 30 40 50 60 MASDIDQKDV DYAAKNLKLT TSLVANKPKD CPPEAPWGAC YRVEINLENT GSKSLNENVE 70 80 90 100 110 120 IYFSSIHRTL GSKSEEFKVE HINGDLHKIT TTEKFKGLKG GKTKSFQVDF MNWIVSNSDF 130 140 150 160 170 180 MPNYYVASEH LEGRNILNTV PIDAVHITEE VSGFTTGIKH TPNQLKRTAN DLLPAATATT 190 200 210 220 230 240 RYEQYSKVKD LGADAVSAHI LPTPLETSVH EGSLNIAQGI NIVSDALPAD QVEALNFRFE 250 260 270 280 290 300 TLGVNTGTGV PVNVTIKADS SKKSGSYTLD VTSSGIRIVG VDKAGAFYGV QSLAGLVTVG 310 320 330 340 350 360 KDTINQVSIN DEPRLDYRGM HMDVSRNFHS KELVFRFLDQ MAAYKMNKFH FHLADDEGWR 370 380 390 400 410 420 LEINGLPELT QVGAHRCHDV EQNKCMMPQL GSGAELPNNG SGYYTREDYK EILAYASARN 430 440 450 460 470 480 IQVIPSMDMP GHSLAAVKSM EARYRKFMAE GDVVKAEMYL LSDPNDTTQY YSIQHYQDNT 490 500 510 520 530 540 INPCMESSFV FMDKVIDEIN KLHKEGGQPL TDYHIGADET AGAWGDSPEC RKMFVAPESG 550 560 570 580 590 600 VKNAKDINGY FINRISHILD AKGLTLGAWN DGLSHKALDA SSLAGNPPKA WVWGTMFWGG 610 620 630 640 650 660 VDQYNSFANK GYDVVVTPPD AYYFDMPYEN DPEERGYYWA TRFNDTKKVF SFMPENVPAN 670 680 690 700 710 720 VEWMTDRMGA KISATTGEKT HDFLGVQGAL WSETIRTDAQ VEYMVLPRMI AVAERGWHKA 730 740 750 760 770 780 SWEEEHKEGI TYTSNVDGHE GTTHLNDNIA TRDADWAHFS NILGYKEMPK LDKAGITYRL 790 800 810 820 830 840 PVLGAVIKNN ILDVVTEFHG VAIQYSLDGK TWHKYDDTKK PQVSTKALVR SVSTNGRTGR AVEVLAK

References

[1]

"Sequence analysis of the beta-N-acetylhexosaminidase gene of Vibrio vulnificus: evidence for a common evolutionary origin of hexosaminidases." Somerville C.C., Colwell R.R. Proc. Natl. Acad. Sci. U.S.A. 90:6751-6755(1993) [PubMed: 8341694] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 27562 / DSM 10143 / IFO 15645 / JCM 3725 / LMG 13545.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L04544 Genomic DNA. Translation: AAA27527.1.
PIR	A48228.
3D structure databases
SMR	Q04786. Positions 6-844.
ModBase	Search...
Protein family/group databases
CAZy	GH20. Glycoside Hydrolase Family 20.
Enzyme and pathway databases
BRENDA	3.2.1.52. 277169.
Family and domain databases
InterPro	IPR008965. Carb_bd. IPR004866. CB_bd_Hex_N. IPR012291. CBD_carb_bd_dom. IPR013812. Glyco_hydro_2/20_Ig-like. IPR001540. Glyco_hydro_20. IPR004867. Glyco_hydro_20_C. IPR015883. Glyco_hydro_20_cat-core. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.290. CBD_carb_bd. 1 hit. G3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR22600. Glyco_hydro_20. 1 hit.
Pfam	PF03173. CHB_HEX. 1 hit. PF03174. CHB_HEX_C. 1 hit. PF00728. Glyco_hydro_20. 1 hit. [Graphical view]
PRINTS	PR00738. GLHYDRLASE20.
ProtoNet	Search...

Entry information

Entry name

HEX_VIBVU

Accession

Primary (citable) accession number: Q04786

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	February 9, 2010
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)