ID   ERG7_CANAL              Reviewed;         728 AA.
AC   Q04782; Q5AM05;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   26-MAY-2009, entry version 56.
DE   RecName: Full=Lanosterol synthase;
DE            EC=5.4.99.7;
DE   AltName: Full=Oxidosqualene--lanosterol cyclase;
DE            Short=OSC;
DE   AltName: Full=2,3-epoxysqualene--lanosterol cyclase;
GN   Name=ERG7; ORFNames=CaO19.1570;
OS   Candida albicans (Yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; mitosporic Saccharomycetales;
OC   Candida.
OX   NCBI_TaxID=5476;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=93252273; PubMed=8486282; DOI=10.1016/0378-1119(93)90631-C;
RA   Roessner C.A., Min C., Hardin S.H., Harris-Haller L.W., McCollum J.C.,
RA   Scott A.I.;
RT   "Sequence of the Candida albicans erg7 gene.";
RL   Gene 127:149-150(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SC5314;
RX   PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA   Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S.,
RA   Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T.,
RA   Davis R.W., Scherer S.;
RT   "The diploid genome sequence of Candida albicans.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
CC   -!- FUNCTION: Catalyzes the cyclization of (S)-2,3 oxidosqualene to
CC       lanosterol, a reaction that forms the sterol nucleus.
CC   -!- CATALYTIC ACTIVITY: (S)-2,3-epoxysqualene = lanosterol.
CC   -!- PATHWAY: Terpene metabolism; lanosterol biosynthesis; lanosterol
CC       from farnesyl-PP: step 3/3.
CC   -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC   -!- SIMILARITY: Contains 3 PFTB repeats.
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DR   EMBL; L04305; AAA34342.1; -; Genomic_DNA.
DR   EMBL; AACQ01000007; EAL03873.1; -; Genomic_DNA.
DR   PIR; JN0664; JN0664.
DR   RefSeq; XP_722612.1; -.
DR   GeneID; 3635761; -.
DR   CGD; CAL0005598; ERG7.
DR   BRENDA; 5.4.99.7; 1124.
DR   GO; GO:0000250; F:lanosterol synthase activity; IEA:EC.
DR   GO; GO:0006694; P:steroid biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR001330; Prenyltrans.
DR   InterPro; IPR018333; Squalene_cyclase.
DR   InterPro; IPR002365; Terpene_synthase_CS.
DR   Pfam; PF00432; Prenyltrans; 3.
DR   TIGRFAMs; TIGR01787; squalene_cyclas; 1.
DR   PROSITE; PS01074; TERPENE_SYNTHASES; 1.
PE   3: Inferred from homology;
KW   Isomerase; Lipid synthesis; Repeat; Steroid biosynthesis.
FT   CHAIN         1    728       Lanosterol synthase.
FT                                /FTId=PRO_0000072654.
FT   REPEAT      117    159       PFTB 1.
FT   REPEAT      561    602       PFTB 2.
FT   REPEAT      611    657       PFTB 3.
FT   ACT_SITE    226    226       Proton acceptor (By similarity).
FT   ACT_SITE    450    450       Proton donor (By similarity).
SQ   SEQUENCE   728 AA;  83715 MW;  98A6289237A074B1 CRC64;
     MYYSEEIGLP KTDISRWRLR SDALGRETWH YLSQSECESE PQSTFVQWLL ESPDFPSPPS
     SDIHTSGEAA RKGADFLKLL QLDNGIFPCQ YKGPMFMTIG YVTANYYSKT EIPEPYRVEM
     IRYIVNTAHP VDGGWGLHSV DKSTCFGTTM NYVCLRLLGM EKDHPVLVKA RKTLHRLGGA
     IKNPHWGKAW LSILNLYEWE GVNPAPPELW RLPYWLPIHP AKWWVHTRAI YLPLGYTSAN
     RVQCELDPLL KEIRNEIYVP SQLPYESIKF GNQRNNVCGV DLYYPHTKIL DFANSILSKW
     EAVRPKWLLN WVNKKVYDLI VKEYQNTEYL CIAPVSFAFN MVVTCHYEGS ESENFKKLQN
     RMNDVLFHGP QGMTVMGTNG VQVWDAAFMV QYFFMTGLVD DPKYHDMIRK SYLFLVRSQF
     TENCVDGSFR DRRKGAWPFS TKEQGYTVSD CTAEAMKAII MVRNHASFAD IRDEIKDENL
     FDAVEVLLQI QNVGEWEYGS FSTYEGIKAP LLLEKLNPAE VFNNIMVEYP YVECTDSSVL
     GLTYFAKYYP DYKPELIQKT ISSAIQYILD SQDNIDGSWY GCWGICYTYA SMFALEALHT
     VGLDYESSSA VKKGCDFLIS KQLPDGGWSE SMKGCETHSY VNGENSLVVQ SAWALIGLIL
     GNYPDEEPIK RGIQFLMKRQ LPTGEWKYED IEGVFNHSCA IEYPSYRFLF PIKALGLYKN
     KYGDKVLV
//