Q04782 (ERG7_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lanosterol synthase
EC=5.4.99.7

Alternative name(s):
2,3-epoxysqualene--lanosterol cyclase
Oxidosqualene--lanosterol cyclase
Short name=OSC

Gene names

Name:	ERG7
ORF Names:	CaO19.1570

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	728 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus.
Catalytic activity	(3S)-2,3-epoxy-2,3-dihydrosqualene = lanosterol.
Pathway	Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 3/3.
Sequence similarities	Belongs to the terpene cyclase/mutase family. Contains 3 PFTB repeats.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis
Domain	Repeat
Molecular function	Isomerase
Gene Ontology (GO)
Biological process	ergosterol biosynthetic process Inferred from genetic interaction Ref.1. Source: CGD
Molecular function	lanosterol synthase activity Inferred from genetic interaction Ref.1. Source: CGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 728	728	Lanosterol synthase		PRO_0000072654
Regions
	Repeat	117 – 159	43	PFTB 1
	Repeat	561 – 602	42	PFTB 2
	Repeat	611 – 657	47	PFTB 3
Sites
	Active site	226	1	Proton acceptor By similarity
	Active site	450	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q04782 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 98A6289237A074B1	FASTA	728	83,715
	10 20 30 40 50 60 MYYSEEIGLP KTDISRWRLR SDALGRETWH YLSQSECESE PQSTFVQWLL ESPDFPSPPS 70 80 90 100 110 120 SDIHTSGEAA RKGADFLKLL QLDNGIFPCQ YKGPMFMTIG YVTANYYSKT EIPEPYRVEM 130 140 150 160 170 180 IRYIVNTAHP VDGGWGLHSV DKSTCFGTTM NYVCLRLLGM EKDHPVLVKA RKTLHRLGGA 190 200 210 220 230 240 IKNPHWGKAW LSILNLYEWE GVNPAPPELW RLPYWLPIHP AKWWVHTRAI YLPLGYTSAN 250 260 270 280 290 300 RVQCELDPLL KEIRNEIYVP SQLPYESIKF GNQRNNVCGV DLYYPHTKIL DFANSILSKW 310 320 330 340 350 360 EAVRPKWLLN WVNKKVYDLI VKEYQNTEYL CIAPVSFAFN MVVTCHYEGS ESENFKKLQN 370 380 390 400 410 420 RMNDVLFHGP QGMTVMGTNG VQVWDAAFMV QYFFMTGLVD DPKYHDMIRK SYLFLVRSQF 430 440 450 460 470 480 TENCVDGSFR DRRKGAWPFS TKEQGYTVSD CTAEAMKAII MVRNHASFAD IRDEIKDENL 490 500 510 520 530 540 FDAVEVLLQI QNVGEWEYGS FSTYEGIKAP LLLEKLNPAE VFNNIMVEYP YVECTDSSVL 550 560 570 580 590 600 GLTYFAKYYP DYKPELIQKT ISSAIQYILD SQDNIDGSWY GCWGICYTYA SMFALEALHT 610 620 630 640 650 660 VGLDYESSSA VKKGCDFLIS KQLPDGGWSE SMKGCETHSY VNGENSLVVQ SAWALIGLIL 670 680 690 700 710 720 GNYPDEEPIK RGIQFLMKRQ LPTGEWKYED IEGVFNHSCA IEYPSYRFLF PIKALGLYKN KYGDKVLV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of the Candida albicans erg7 gene." Roessner C.A., Min C., Hardin S.H., Harris-Haller L.W., McCollum J.C., Scott A.I. Gene 127:149-150(1993) [PubMed: 8486282] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed: 15123810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L04305 Genomic DNA. Translation: AAA34342.1. AACQ01000007 Genomic DNA. Translation: EAL03873.1.
PIR	JN0664.
RefSeq	XP_722612.1. XM_717519.1.
3D structure databases
ProteinModelPortal	Q04782.
ModBase	Search...
Protein-protein interaction databases
STRING	Q04782.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3635761.
KEGG	cal:CaO19.1570.
Organism-specific databases
CGD	CAL0005598. ERG7.
Phylogenomic databases
PhylomeDB	Q04782.
Family and domain databases
InterPro	IPR001330. Prenyltrans. IPR018333. Squalene_cyclase. IPR002365. Terpene_synthase_CS. IPR008930. Terpenoid_cyclase/PrenylTrfase. [Graphical view]
KO	K01852.
Pfam	PF00432. Prenyltrans. 3 hits. [Graphical view]
SUPFAM	SSF48239. Terp_cyc_toroid. 2 hits.
TIGRFAMs	TIGR01787. Squalene_cyclas. 1 hit.
PROSITE	PS01074. TERPENE_SYNTHASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ERG7_CANAL

Accession

Primary (citable) accession number: Q04782
Secondary accession number(s): Q5AM05

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program