Protein kinase C theta type - Homo sapiens (Human)

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

Alternative products

Sequence annotation (Features)

Sequences

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

Alternative products

Sequence annotation (Features)

Sequences

Molecule processing

Regions

Sites

Amino acid modifications

Natural variations

Experimental info

Secondary structure

Keywords

Gene Ontology (GO)

With

Entry

#Exp.

IntAct

Notes

Molecule processing

Regions

Sites

Amino acid modifications

Natural variations

Experimental info

Secondary structure

Protein names

Recommended name:
    Protein kinase C theta type
    EC=2.7.11.13
Alternative name(s):
    nPKC-theta

Gene names

Name:	PRKCQ
Synonyms:	PRKCT

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Sequence length	706 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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Function	This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium.
Enzyme regulation	Three specific sites; Thr-538 (activation loop of the kinase domain), Ser-676 (turn motif) and Ser-695 (hydrophobic region), need to be phosphorylated for its full activation.
Subunit structure	Interacts with TXNL2/PICOT.
Tissue specificity	Skeletal muscle, megakaryoblastic cells and platelets.
Domain	The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
Post-translational modification	Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 C2 domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain.

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Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Phorbol-ester binding Repeat Zinc-finger
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	intracellular signaling cascade Ref.1 Non-traceable author statement. Source: UniProtKB protein amino acid phosphorylation Non-traceable author statement. Source: UniProtKB regulation of cell growth Ref.3 Non-traceable author statement. Source: UniProtKB
Molecular function	ATP binding Non-traceable author statement. Source: UniProtKB diacylglycerol binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Ref.7 Inferred from physical interaction. Source: IntAct protein kinase C activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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With	Entry	#Exp.	IntAct
BTK	Q06187	2	EBI-374762,EBI-624835
FYN	P06241	6	EBI-374762,EBI-515315
GLRX3	O76003	2	EBI-374762,EBI-374781
PRKCZ	Q05513	2	EBI-374762,EBI-295351

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Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Chain

1 – 706

706

Protein kinase C theta type

PRO_0000055708

Domain

8 – 123

116

C2

Domain

380 – 634

255

Protein kinase

Domain

635 – 706

72

AGC-kinase C-terminal

Zinc finger

159 – 209

51

Phorbol-ester/DAG-type 1

Zinc finger

231 – 281

51

Phorbol-ester/DAG-type 2

Nucleotide binding

386 – 394

9

ATP By similarity

Active site

504

1

Proton acceptor By similarity

Binding site

409

1

ATP

Modified residue

219

1

Phosphothreonine; by autocatalysis

Modified residue

538

1

Phosphothreonine

Modified residue

676

1

Phosphoserine

Modified residue

685

1

Phosphoserine

Modified residue

695

1

Phosphoserine

Alternative sequence

550 – 612

63

Missing in isoform 2.

VSP_017294

Natural variant

240

1

K → N in a colorectal adenocarcinoma sample; somatic mutation.

VAR_042319

Natural variant

306

1

D → V

VAR_042320

Natural variant

330

1

P → L: dbSNP rs2236379.

VAR_020401

Natural variant

354

1

D → N

VAR_042321

Mutagenesis

219

1

T → A: Loss of transactivation of the IL2 promoter and translocation to the plasma membrane. No effect on kinase activity

Mutagenesis

409

1

K → A: Loss of kinase activity

Mutagenesis

538

1

T → A: Loss of kinase activity

Mutagenesis

676

1

S → A: Reduction in kinase activity

Mutagenesis

695

1

S → A: Reduction in kinase activity

Sequence conflict

700

1

G → R Ref.1 Ref.4

1

.

706

Helix Strand Turn

Details...

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Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified March 27, 2002. Version 3. Checksum: B3C53AB892D5210A Show »	FASTA	706	81,865
10 20 30 40 50 60 MSPFLRIGLS NFDCGSCQSC QGEAVNPYCA VLVKEYVESE NGQMYIQKKP TMYPPWDSTF 70 80 90 100 110 120 DAHINKGRVM QIIVKGKNVD LISETTVELY SLAERCRKNN GKTEIWLELK PQGRMLMNAR 130 140 150 160 170 180 YFLEMSDTKD MNEFETEGFF ALHQRRGAIK QAKVHHVKCH EFTATFFPQP TFCSVCHEFV 190 200 210 220 230 240 WGLNKQGYQC RQCNAAIHKK CIDKVIAKCT GSAINSRETM FHKERFKIDM PHRFKVYNYK 250 260 270 280 290 300 SPTFCEHCGT LLWGLARQGL KCDACGMNVH HRCQTKVANL CGINQKLMAE ALAMIESTQQ 310 320 330 340 350 360 ARCLRDTEQI FREGPVEIGL PCSIKNEARP PCLPTPGKRE PQGISWESPL DEVDKMCHLP 370 380 390 400 410 420 EPELNKERPS LQIKLKIEDF ILHKMLGKGS FGKVFLAEFK KTNQFFAIKA LKKDVVLMDD 430 440 450 460 470 480 DVECTMVEKR VLSLAWEHPF LTHMFCTFQT KENLFFVMEY LNGGDLMYHI QSCHKFDLSR 490 500 510 520 530 540 ATFYAAEIIL GLQFLHSKGI VYRDLKLDNI LLDKDGHIKI ADFGMCKENM LGDAKTNTFC 550 560 570 580 590 600 GTPDYIAPEI LLGQKYNHSV DWWSFGVLLY EMLIGQSPFH GQDEEELFHS IRMDNPFYPR 610 620 630 640 650 660 WLEKEAKDLL VKLFVREPEK RLGVRGDIRQ HPLFREINWE ELERKEIDPP FRPKVKSPFD 670 680 690 700 CSNFDKEFLN EKPRLSFADR ALINSMDQNM FRNFSFMNPG MERLIS « Hide
Isoform 2 [UniParc]. Checksum: A3B688EBE8809B9E Show »		643	74,287
10 20 30 40 50 60 MSPFLRIGLS NFDCGSCQSC QGEAVNPYCA VLVKEYVESE NGQMYIQKKP TMYPPWDSTF 70 80 90 100 110 120 DAHINKGRVM QIIVKGKNVD LISETTVELY SLAERCRKNN GKTEIWLELK PQGRMLMNAR 130 140 150 160 170 180 YFLEMSDTKD MNEFETEGFF ALHQRRGAIK QAKVHHVKCH EFTATFFPQP TFCSVCHEFV 190 200 210 220 230 240 WGLNKQGYQC RQCNAAIHKK CIDKVIAKCT GSAINSRETM FHKERFKIDM PHRFKVYNYK 25

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q04759-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q04759-2)

The sequence of this isoform differs from the canonical sequence as follows:
550-612: Missing.