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Reviewed, UniProtKB/Swiss-Prot Q04759 (KPCT_HUMAN)

Last modified November 25, 2008. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Protein kinase C theta type
    EC=2.7.11.13
Alternative name(s):
    nPKC-theta
Gene names
Name: PRKCQ
Synonyms: PRKCT
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length706 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.

PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium.

Enzyme regulation

Three specific sites; Thr-538 (activation loop of the kinase domain), Ser-676 (turn motif) and Ser-695 (hydrophobic region), need to be phosphorylated for its full activation.

Subunit structure

Interacts with TXNL2/PICOT.

Tissue specificity

Skeletal muscle, megakaryoblastic cells and platelets.

Domain

The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.

Post-translational modification

Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 C2 domain.

Contains 2 phorbol-ester/DAG-type zinc fingers.

Contains 1 protein kinase domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q04759-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q04759-2)

The sequence of this isoform differs from the canonical sequence as follows:
     550-612: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 706706Protein kinase C theta type
PRO_0000055708

Regions

Domain8 – 123116C2
Domain380 – 634255Protein kinase
Domain635 – 70672AGC-kinase C-terminal
Zinc finger159 – 20951Phorbol-ester/DAG-type 1
Zinc finger231 – 28151Phorbol-ester/DAG-type 2
Nucleotide binding386 – 3949ATP By similarity

Sites

Active site5041Proton acceptor By similarity
Binding site4091ATP

Amino acid modifications

Modified residue2191Phosphothreonine; by autocatalysis
Modified residue5381Phosphothreonine
Modified residue6761Phosphoserine
Modified residue6851Phosphoserine
Modified residue6951Phosphoserine

Natural variations

Alternative sequence550 – 61263Missing in isoform 2.
VSP_017294
Natural variant2401K → N in a colorectal adenocarcinoma sample; somatic mutation.
VAR_042319
Natural variant3061D → V
VAR_042320
Natural variant3301P → L: dbSNP rs2236379.
VAR_020401
Natural variant3541D → N
VAR_042321

Experimental info

Mutagenesis2191T → A: Loss of transactivation of the IL2 promoter and translocation to the plasma membrane. No effect on kinase activity
Mutagenesis4091K → A: Loss of kinase activity
Mutagenesis5381T → A: Loss of kinase activity
Mutagenesis6761S → A: Reduction in kinase activity
Mutagenesis6951S → A: Reduction in kinase activity
Sequence conflict7001G → R Ref.1 Ref.4

Secondary structure

.......................................................................................... 706
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 27, 2002. Version 3.
Checksum: B3C53AB892D5210A

FASTA70681,865
        10         20         30         40         50         60 
MSPFLRIGLS NFDCGSCQSC QGEAVNPYCA VLVKEYVESE NGQMYIQKKP TMYPPWDSTF 

        70         80         90        100        110        120 
DAHINKGRVM QIIVKGKNVD LISETTVELY SLAERCRKNN GKTEIWLELK PQGRMLMNAR 

       130        140        150        160        170        180 
YFLEMSDTKD MNEFETEGFF ALHQRRGAIK QAKVHHVKCH EFTATFFPQP TFCSVCHEFV 

       190        200        210        220        230        240 
WGLNKQGYQC RQCNAAIHKK CIDKVIAKCT GSAINSRETM FHKERFKIDM PHRFKVYNYK 

       250        260        270        280        290        300 
SPTFCEHCGT LLWGLARQGL KCDACGMNVH HRCQTKVANL CGINQKLMAE ALAMIESTQQ 

       310        320        330        340        350        360 
ARCLRDTEQI FREGPVEIGL PCSIKNEARP PCLPTPGKRE PQGISWESPL DEVDKMCHLP 

       370        380        390        400        410        420 
EPELNKERPS LQIKLKIEDF ILHKMLGKGS FGKVFLAEFK KTNQFFAIKA LKKDVVLMDD 

       430        440        450        460        470        480 
DVECTMVEKR VLSLAWEHPF LTHMFCTFQT KENLFFVMEY LNGGDLMYHI QSCHKFDLSR 

       490        500        510        520        530        540 
ATFYAAEIIL GLQFLHSKGI VYRDLKLDNI LLDKDGHIKI ADFGMCKENM LGDAKTNTFC 

       550        560        570        580        590        600 
GTPDYIAPEI LLGQKYNHSV DWWSFGVLLY EMLIGQSPFH GQDEEELFHS IRMDNPFYPR 

       610        620        630        640        650        660 
WLEKEAKDLL VKLFVREPEK RLGVRGDIRQ HPLFREINWE ELERKEIDPP FRPKVKSPFD 

       670        680        690        700 
CSNFDKEFLN EKPRLSFADR ALINSMDQNM FRNFSFMNPG MERLIS 

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Isoform 2 [UniParc].

Checksum: A3B688EBE8809B9E
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