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Protein

Hepatocyte growth factor activator

Gene

HGFAC

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form.

Caution

It is uncertain whether Met-1 is the initiator.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei447Charge relay system1 Publication1
Active sitei497Charge relay system1 Publication1
Active sitei598Charge relay system1 Publication1

GO - Molecular functioni

  • serine-type endopeptidase activity Source: Reactome
  • serine-type peptidase activity Source: ProtInc

GO - Biological processi

  • proteolysis Source: ProtInc

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Enzyme and pathway databases

ReactomeiR-HSA-6806942 MET Receptor Activation

Protein family/group databases

MEROPSiS01.228

Names & Taxonomyi

Protein namesi
Recommended name:
Hepatocyte growth factor activator (EC:3.4.21.-)
Short name:
HGF activator
Short name:
HGFA
Cleaved into the following 2 chains:
Gene namesi
Name:HGFAC
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000109758.8
HGNCiHGNC:4894 HGFAC
MIMi604552 gene
neXtProtiNX_Q04756

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi3083
OpenTargetsiENSG00000109758
PharmGKBiPA29270

Chemistry databases

ChEMBLiCHEMBL3351190

Polymorphism and mutation databases

BioMutaiHGFAC
DMDMi547643

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 351 PublicationAdd BLAST35
PropeptideiPRO_000002791136 – 372Removed in mature formAdd BLAST337
ChainiPRO_0000027912373 – 407Hepatocyte growth factor activator short chainAdd BLAST35
ChainiPRO_0000027913408 – 655Hepatocyte growth factor activator long chainAdd BLAST248

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi48N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi108 ↔ 133By similarity
Disulfide bondi122 ↔ 148By similarity
Disulfide bondi164 ↔ 175By similarity
Disulfide bondi169 ↔ 186By similarity
Disulfide bondi188 ↔ 197By similarity
Disulfide bondi202 ↔ 230By similarity
Disulfide bondi228 ↔ 237By similarity
Disulfide bondi245 ↔ 256By similarity
Disulfide bondi250 ↔ 267By similarity
Disulfide bondi269 ↔ 278By similarity
Disulfide bondi286 ↔ 367By similarity
Glycosylationi290N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi307 ↔ 349By similarity
Disulfide bondi338 ↔ 362By similarity
Disulfide bondi394 ↔ 521Interchain (between short and long chains)
Disulfide bondi432 ↔ 448
Disulfide bondi440 ↔ 510
Glycosylationi468N-linked (GlcNAc...) asparagine3 Publications1
Glycosylationi492N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi535 ↔ 604
Glycosylationi546N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi567 ↔ 583
Disulfide bondi594 ↔ 622

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiQ04756
PaxDbiQ04756
PeptideAtlasiQ04756
PRIDEiQ04756

PTM databases

iPTMnetiQ04756
PhosphoSitePlusiQ04756

Miscellaneous databases

PMAP-CutDBiQ04756

Expressioni

Tissue specificityi

Liver.

Gene expression databases

BgeeiENSG00000109758
CleanExiHS_HGFAC
ExpressionAtlasiQ04756 baseline and differential
GenevisibleiQ04756 HS

Organism-specific databases

HPAiHPA058279
HPA059076

Interactioni

Subunit structurei

Heterodimer of a short chain and a long chain linked by a disulfide bond.2 Publications

Protein-protein interaction databases

BioGridi10933112 interactors.
DIPiDIP-6022N
IntActiQ04756 5 interactors.
STRINGi9606.ENSP00000372224

Chemistry databases

BindingDBiQ04756

Structurei

Secondary structure

1655
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi422 – 427Combined sources6
Beta strandi430 – 438Combined sources9
Beta strandi441 – 444Combined sources4
Helixi446 – 449Combined sources4
Helixi455 – 457Combined sources3
Beta strandi458 – 463Combined sources6
Beta strandi475 – 477Combined sources3
Beta strandi479 – 484Combined sources6
Beta strandi490 – 492Combined sources3
Turni493 – 496Combined sources4
Beta strandi499 – 503Combined sources5
Beta strandi506 – 508Combined sources3
Beta strandi509 – 511Combined sources3
Beta strandi514 – 516Combined sources3
Beta strandi534 – 540Combined sources7
Beta strandi543 – 546Combined sources4
Beta strandi555 – 561Combined sources7
Helixi564 – 567Combined sources4
Turni570 – 573Combined sources4
Helixi574 – 576Combined sources3
Beta strandi581 – 585Combined sources5
Beta strandi587 – 589Combined sources3
Turni595 – 599Combined sources5
Beta strandi601 – 606Combined sources6
Beta strandi609 – 618Combined sources10
Turni621 – 623Combined sources3
Beta strandi625 – 627Combined sources3
Beta strandi629 – 633Combined sources5
Helixi634 – 637Combined sources4
Helixi638 – 645Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YBWX-ray2.70A/B373-655[»]
1YC0X-ray2.60A373-655[»]
2R0KX-ray3.51A373-655[»]
2R0LX-ray2.20A408-655[»]
B373-407[»]
2WUBX-ray2.90A/C408-655[»]
B/D373-407[»]
2WUCX-ray2.70A408-654[»]
B373-407[»]
3K2UX-ray2.35A408-655[»]
B373-407[»]
ProteinModelPortaliQ04756
SMRiQ04756
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ04756

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini103 – 150Fibronectin type-IIPROSITE-ProRule annotationAdd BLAST48
Domaini160 – 198EGF-like 1PROSITE-ProRule annotationAdd BLAST39
Domaini200 – 240Fibronectin type-IPROSITE-ProRule annotationAdd BLAST41
Domaini241 – 279EGF-like 2PROSITE-ProRule annotationAdd BLAST39
Domaini286 – 367KringlePROSITE-ProRule annotationAdd BLAST82
Domaini408 – 646Peptidase S1PROSITE-ProRule annotationAdd BLAST239

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Kringle, Repeat, Signal

Phylogenomic databases

eggNOGiKOG1217 Eukaryota
KOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00760000119133
HOGENOMiHOG000237314
HOVERGENiHBG004345
InParanoidiQ04756
KOiK09631
PhylomeDBiQ04756
TreeFamiTF329901

Family and domain databases

CDDicd00062 FN2, 1 hit
cd00190 Tryp_SPc, 1 hit
Gene3Di2.10.10.101 hit
2.40.20.101 hit
InterProiView protein in InterPro
IPR014394 Coagulation_fac_XII/HGFA
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000083 Fibronectin_type1
IPR000562 FN_type2_dom
IPR036943 FN_type2_sf
IPR000001 Kringle
IPR013806 Kringle-like
IPR018056 Kringle_CS
IPR038178 Kringle_sf
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00008 EGF, 1 hit
PF00039 fn1, 1 hit
PF00040 fn2, 1 hit
PF00051 Kringle, 1 hit
PF00089 Trypsin, 1 hit
PIRSFiPIRSF001146 Factor_XII_HGFA, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00181 EGF, 2 hits
SM00058 FN1, 1 hit
SM00059 FN2, 1 hit
SM00130 KR, 1 hit
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
SSF57440 SSF57440, 2 hits
PROSITEiView protein in PROSITE
PS00022 EGF_1, 2 hits
PS01186 EGF_2, 1 hit
PS50026 EGF_3, 2 hits
PS01253 FN1_1, 1 hit
PS51091 FN1_2, 1 hit
PS00023 FN2_1, 1 hit
PS51092 FN2_2, 1 hit
PS00021 KRINGLE_1, 1 hit
PS50070 KRINGLE_2, 1 hit
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q04756-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRWAWVPSP WPPPGLGPFL LLLLLLLLLP RGFQPQPGGN RTESPEPNAT
60 70 80 90 100
ATPAIPTILV TSVTSETPAT SAPEAEGPQS GGLPPPPRAV PSSSSPQAQA
110 120 130 140 150
LTEDGRPCRF PFRYGGRMLH ACTSEGSAHR KWCATTHNYD RDRAWGYCVE
160 170 180 190 200
ATPPPGGPAA LDPCASGPCL NGGSCSNTQD PQSYHCSCPR AFTGKDCGTE
210 220 230 240 250
KCFDETRYEY LEGGDRWARV RQGHVEQCEC FGGRTWCEGT RHTACLSSPC
260 270 280 290 300
LNGGTCHLIV ATGTTVCACP PGFAGRLCNI EPDERCFLGN GTGYRGVAST
310 320 330 340 350
SASGLSCLAW NSDLLYQELH VDSVGAAALL GLGPHAYCRN PDNDERPWCY
360 370 380 390 400
VVKDSALSWE YCRLEACESL TRVQLSPDLL ATLPEPASPG RQACGRRHKK
410 420 430 440 450
RTFLRPRIIG GSSSLPGSHP WLAAIYIGDS FCAGSLVHTC WVVSAAHCFS
460 470 480 490 500
HSPPRDSVSV VLGQHFFNRT TDVTQTFGIE KYIPYTLYSV FNPSDHDLVL
510 520 530 540 550
IRLKKKGDRC ATRSQFVQPI CLPEPGSTFP AGHKCQIAGW GHLDENVSGY
560 570 580 590 600
SSSLREALVP LVADHKCSSP EVYGADISPN MLCAGYFDCK SDACQGDSGG
610 620 630 640 650
PLACEKNGVA YLYGIISWGD GCGRLHKPGV YTRVANYVDW INDRIRPPRR

LVAPS
Length:655
Mass (Da):70,682
Last modified:June 1, 1994 - v1
Checksum:i2CF72F1E1B862ED7
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051851218A → S. Corresponds to variant dbSNP:rs3748034Ensembl.1
Natural variantiVAR_033651225V → M. Corresponds to variant dbSNP:rs16844370Ensembl.1
Natural variantiVAR_033652231F → L1 PublicationCorresponds to variant dbSNP:rs1987546Ensembl.1
Natural variantiVAR_024294509R → H. Corresponds to variant dbSNP:rs16844401Ensembl.1
Natural variantiVAR_024295644R → Q. Corresponds to variant dbSNP:rs2498323Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14012 mRNA Translation: BAA03113.1
BC112190 mRNA Translation: AAI12191.1
D50030 Genomic DNA Translation: BAA74450.1
AL590235 Genomic DNA No translation available.
CH471131 Genomic DNA Translation: EAW82464.1
BC112192 mRNA Translation: AAI12193.1
CCDSiCCDS3369.1
PIRiA46688
RefSeqiNP_001284368.1, NM_001297439.1
NP_001519.1, NM_001528.3
UniGeneiHs.104

Genome annotation databases

EnsembliENST00000382774; ENSP00000372224; ENSG00000109758
GeneIDi3083
KEGGihsa:3083
UCSCiuc003ghc.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiHGFA_HUMAN
AccessioniPrimary (citable) accession number: Q04756
Secondary accession number(s): Q14726, Q2M1W7, Q53X47
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: March 28, 2018
This is version 172 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome