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Protein

Methylosome subunit pICln

Gene

Clns1a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Chaperone that regulates the assembly of spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. May also indirectly participate in cellular volume control by activation of a swelling-induced chloride conductance pathway (By similarity).By similarity

GO - Biological processi

  • cell volume homeostasis Source: RGD
  • chloride transport Source: RGD
  • spliceosomal snRNP assembly Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Methylosome subunit pICln
Alternative name(s):
Chloride channel, nucleotide sensitive 1A
Chloride conductance regulatory protein ICln
Short name:
I(Cln)
Gene namesi
Name:Clns1a
Synonyms:Icln, Rcl1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi61884. Clns1a.

Subcellular locationi

GO - Cellular componenti

  • cytoskeleton Source: UniProtKB-SubCell
  • cytosol Source: RGD
  • methylosome Source: UniProtKB
  • nucleus Source: UniProtKB-SubCell
  • pICln-Sm protein complex Source: UniProtKB
  • plasma membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 236235Methylosome subunit pIClnPRO_0000185157Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei95 – 951PhosphoserineCombined sources
Modified residuei143 – 1431PhosphoserineBy similarity
Modified residuei192 – 1921PhosphoserineBy similarity
Modified residuei194 – 1941PhosphoserineBy similarity
Modified residuei197 – 1971PhosphoserineBy similarity
Modified residuei209 – 2091PhosphoserineBy similarity
Modified residuei222 – 2221PhosphothreonineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ04753.
PRIDEiQ04753.

PTM databases

iPTMnetiQ04753.
PhosphoSiteiQ04753.

Expressioni

Tissue specificityi

Expressed in most tissues.

Interactioni

Subunit structurei

Component of the methylosome, a 20S complex containing at least CLNS1A/pICln, PRMT5/SKB1 and WDR77/MEP50; may mediate SNRPD1 and SNRPD3 methylation. Forms a 6S pICln-Sm complex composed of CLNS1A/pICln, SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG; ring-like structure where CLNS1A/pICln mimics additional Sm proteins and which is unable to assemble into the core snRNP. Interacts with LSM10 and LSM11 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017702.

Structurei

3D structure databases

ProteinModelPortaliQ04753.
SMRiQ04753. Positions 19-134.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3238. Eukaryota.
ENOG4111R94. LUCA.
HOVERGENiHBG003108.
InParanoidiQ04753.
KOiK05019.

Family and domain databases

InterProiIPR003521. ICln.
[Graphical view]
PRINTSiPR01348. ICLNCHANNEL.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q04753-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFLKSFPPP GSADGLRLQQ PDTEAVLNGK GLGTGTLYIA ESRLSWLDGS
60 70 80 90 100
GLGFSLEYPT ISLHAVSRDP NAYPQEHLYV MVNARFGEES KEPFSDEDED
110 120 130 140 150
DNDDVEPISE FRFVPSDKSA LEAMFTAMCE CQALHPDPED EDSDDYDGEE
160 170 180 190 200
YDVEAHEQGQ GDIPTFYTYE EGLSHLTAEG QATLERLEGM LSQSVSSQYN
210 220 230
MAGVRTEDSV RTYEDGMEVE TTPTVAGQFE DADVDH
Length:236
Mass (Da):26,092
Last modified:June 1, 1994 - v1
Checksum:i7430F2B96B5212A7
GO

Sequence cautioni

The sequence AAC37642.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17698 mRNA. Translation: BAA04561.1.
D13985 mRNA. Translation: BAA03092.1.
L26450 mRNA. Translation: AAC37642.1. Different initiation.
PIRiB53014.
RefSeqiNP_113907.1. NM_031719.1.
XP_006229854.1. XM_006229792.2.
UniGeneiRn.4089.

Genome annotation databases

GeneIDi65160.
KEGGirno:65160.
UCSCiRGD:61884. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17698 mRNA. Translation: BAA04561.1.
D13985 mRNA. Translation: BAA03092.1.
L26450 mRNA. Translation: AAC37642.1. Different initiation.
PIRiB53014.
RefSeqiNP_113907.1. NM_031719.1.
XP_006229854.1. XM_006229792.2.
UniGeneiRn.4089.

3D structure databases

ProteinModelPortaliQ04753.
SMRiQ04753. Positions 19-134.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017702.

PTM databases

iPTMnetiQ04753.
PhosphoSiteiQ04753.

Proteomic databases

PaxDbiQ04753.
PRIDEiQ04753.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi65160.
KEGGirno:65160.
UCSCiRGD:61884. rat.

Organism-specific databases

CTDi1207.
RGDi61884. Clns1a.

Phylogenomic databases

eggNOGiKOG3238. Eukaryota.
ENOG4111R94. LUCA.
HOVERGENiHBG003108.
InParanoidiQ04753.
KOiK05019.

Miscellaneous databases

PROiQ04753.

Family and domain databases

InterProiIPR003521. ICln.
[Graphical view]
PRINTSiPR01348. ICLNCHANNEL.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and expression of a rat cDNA encoding MDCK-type chloride channel."
    Abe T., Takeuchi K., Ishii K., Abe K.
    Biochim. Biophys. Acta 1173:353-356(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Kidney.
  2. "Tissue expression of mRNA of chloride channel from MDCK cells and its regulation by protein kinases."
    Ishibashi K., Sasaki S., Uchida S., Imai T., Marumo F.
    Biochem. Biophys. Res. Commun. 192:561-567(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Molecular characterization of a swelling-induced chloride conductance regulatory protein, pICln."
    Krapivinsky G.B., Ackerman M.J., Gordon E.A., Krapivinsky L.D., Clapham D.E.
    Cell 76:439-448(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Neonatal heart atrium.
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-95, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiICLN_RAT
AccessioniPrimary (citable) accession number: Q04753
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: July 6, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be a chloride channel.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.