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Q04752 (STF1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Steroidogenic factor 1

Short name=SF-1
Short name=STF-1
Alternative name(s):
Adrenal 4-binding protein
Fushi tarazu factor homolog 1
Nuclear receptor subfamily 5 group A member 1
Steroid hormone receptor Ad4BP
Gene names
Name:NR5A1
Synonyms:AD4BP, FTZF1
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length461 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation By similarity.

Subunit structure

Binds DNA as a monomer. Part of a complex consisting of SFPQ, NONO and NR5A1. Interacts with NR0B2. Interacts with DGKQ and CDK7. Binds to and activated by HIPK3 By similarity.

Subcellular location

Nucleus.

Tissue specificity

Adrenal, ovary, testis, placenta, adipocyte, and brain.

Post-translational modification

May be regulated by phosphorylation and dephosphorylation.

Acetylation stimulates the transcriptional activity By similarity.

Sumoylation reduces CDK7-mediated phosophorylation on Ser-203 By similarity.

Phosphorylated on Ser-203 by CDK7. This phosphorylation promotes transcriptional activity By similarity.

Sequence similarities

Belongs to the nuclear hormone receptor family. NR5 subfamily.

Contains 1 nuclear receptor DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainZinc-finger
   LigandDNA-binding
Lipid-binding
Metal-binding
Zinc
   Molecular functionActivator
Receptor
   PTMAcetylation
Isopeptide bond
Phosphoprotein
Ubl conjugation
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processadrenal gland development

Inferred from electronic annotation. Source: Ensembl

cell differentiation

Inferred from electronic annotation. Source: Ensembl

hormone metabolic process

Inferred from electronic annotation. Source: Ensembl

luteinization

Inferred from electronic annotation. Source: Ensembl

maintenance of protein location in nucleus

Inferred from electronic annotation. Source: Ensembl

male gonad development

Inferred from electronic annotation. Source: Ensembl

multicellular organismal aging

Inferred from electronic annotation. Source: Ensembl

negative regulation of female gonad development

Inferred from electronic annotation. Source: Ensembl

positive regulation of male gonad development

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

tissue development

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentnucleus

Inferred from mutant phenotype PubMed 10567391. Source: UniProtKB

   Molecular_functionDNA binding

Inferred from sequence or structural similarity. Source: HGNC

RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: Ensembl

chromatin binding

Inferred from electronic annotation. Source: Ensembl

phospholipid binding

Inferred from electronic annotation. Source: Ensembl

protein binding

Inferred from physical interaction PubMed 10567391. Source: UniProtKB

sequence-specific DNA binding

Inferred from mutant phenotype PubMed 10567391. Source: UniProtKB

steroid hormone receptor activity

Inferred from direct assay Ref.1. Source: UniProtKB

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

EDF1O60869-14EBI-850837,EBI-781310From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 461461Steroidogenic factor 1
PRO_0000053728

Regions

DNA binding10 – 8576Nuclear receptor
Zinc finger13 – 3321NR C4-type
Zinc finger49 – 7325NR C4-type
Region230 – 461232Important for dimerization
Region260 – 34788Ligand-binding By similarity

Sites

Binding site3411Lipid headgroup; via amide nitrogen By similarity
Binding site4361Lipid headgroup By similarity
Binding site4401Lipid headgroup By similarity

Amino acid modifications

Modified residue2031Phosphoserine; by CDK7 By similarity
Cross-link119Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Cross-link194Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Experimental info

Sequence conflict64 – 652Missing in AAB23574. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q04752 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 910747C467ABBD6E

FASTA46151,613
        10         20         30         40         50         60 
MDYSYDEDLD ELCPVCGDKV SGYHYGLLTC ESCKGFFKRT VQNNKHYTCT ESQSCKIDKT 

        70         80         90        100        110        120 
QRKRCPFCRF QKCLTVGMRL EAVRADRMRG GRNKFGPMYK RDRALKQQKK AQIRANGFKL 

       130        140        150        160        170        180 
ETGPPVGVPP PPPPPPDYML PHGLHASEPK GLASGPPAGP LGDFGAPALP MAVPSAHGPL 

       190        200        210        220        230        240 
AGYLYPAFPG RAIKSEYPEP YASPPQPGPP YGYPEPFSGG PGVPELILQL LQLEPDEDQV 

       250        260        270        280        290        300 
RARIVGCLQE PAKGRPDQPA PFSLLCRMAD QTFISIVDWA RRCMVFKELE VADQMTLLQN 

       310        320        330        340        350        360 
CWSELLVFDH IYRQIQHGKE GSILLVTGQE VELTTVAAQA GSLLHSLVLR AQELVLQLHA 

       370        380        390        400        410        420 
LQLDRQEFVC LKFLILFSLD VKFLNNHSLV KEAQEKANAA LLDYTLCHYP HCGDKFQQLL 

       430        440        450        460 
LCLVEVRALS MQAKEYLYHK HLGNEMPRNN LLIEMLQAKQ T 

« Hide

References

[1]"Ad4BP regulating steroidogenic P-450 gene is a member of steroid hormone receptor superfamily."
Honda S., Morohashi K., Nomura M., Takeya H., Kitajima M., Omura T.
J. Biol. Chem. 268:7494-7502(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 120-180.
Tissue: Adrenal cortex.
[2]"Steroidogenic factor I, a key regulator of steroidogenic enzyme expression, is the mouse homolog of fushi tarazu-factor I."
Lala D.S., Rice D.A., Parker K.L.
Mol. Endocrinol. 6:1249-1258(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-81.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D13569 mRNA. Translation: BAA02764.1.
S45997 mRNA. Translation: AAB23574.2.
PIRA46077.
RefSeqNP_776828.1. NM_174403.2.
UniGeneBt.7422.

3D structure databases

ProteinModelPortalQ04752.
SMRQ04752. Positions 10-111, 236-460.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ04752. 1 interaction.
STRING9913.ENSBTAP00000011869.

Proteomic databases

PRIDEQ04752.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000011869; ENSBTAP00000011869; ENSBTAG00000009017.
GeneID281948.
KEGGbta:281948.

Organism-specific databases

CTD2516.

Phylogenomic databases

eggNOGNOG240365.
GeneTreeENSGT00750000117458.
HOGENOMHOG000063718.
HOVERGENHBG106677.
InParanoidQ04752.
KOK08560.
OMALQEPAKG.
OrthoDBEOG7BS4B9.
TreeFamTF350737.

Family and domain databases

Gene3D1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProIPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR016355. Steroidogenic_factor_1.
IPR001723. Str_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PIRSFPIRSF002530. Nuc_orph_FTZ-F1. 1 hit.
PRINTSPR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMSSF48508. SSF48508. 2 hits.
PROSITEPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20805824.

Entry information

Entry nameSTF1_BOVIN
AccessionPrimary (citable) accession number: Q04752
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: June 11, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families