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Protein

Steroidogenic factor 1

Gene

NR5A1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei341 – 3411Lipid headgroup; via amide nitrogenBy similarity
Binding sitei436 – 4361Lipid headgroupBy similarity
Binding sitei440 – 4401Lipid headgroupBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi10 – 8576Nuclear receptorPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri13 – 3321NR C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri49 – 7325NR C4-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. chromatin binding Source: Ensembl
  2. DNA binding Source: HGNC
  3. phospholipid binding Source: Ensembl
  4. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: Ensembl
  5. sequence-specific DNA binding Source: UniProtKB
  6. steroid hormone receptor activity Source: UniProtKB
  7. zinc ion binding Source: InterPro

GO - Biological processi

  1. adrenal gland development Source: Ensembl
  2. cell differentiation Source: Ensembl
  3. hormone metabolic process Source: Ensembl
  4. luteinization Source: Ensembl
  5. maintenance of protein location in nucleus Source: Ensembl
  6. male gonad development Source: Ensembl
  7. multicellular organismal aging Source: Ensembl
  8. negative regulation of female gonad development Source: Ensembl
  9. positive regulation of male gonad development Source: Ensembl
  10. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  11. tissue development Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Activator, Receptor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Lipid-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_276414. Nuclear Receptor transcription pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Steroidogenic factor 1
Short name:
SF-1
Short name:
STF-1
Alternative name(s):
Adrenal 4-binding protein
Fushi tarazu factor homolog 1
Nuclear receptor subfamily 5 group A member 1
Steroid hormone receptor Ad4BP
Gene namesi
Name:NR5A1
Synonyms:AD4BP, FTZF1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 11

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 461461Steroidogenic factor 1PRO_0000053728Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki119 – 119Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki194 – 194Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei203 – 2031Phosphoserine; by CDK7By similarity

Post-translational modificationi

May be regulated by phosphorylation and dephosphorylation.
Acetylation stimulates the transcriptional activity.By similarity
Sumoylation reduces CDK7-mediated phosophorylation on Ser-203.By similarity
Phosphorylated on Ser-203 by CDK7. This phosphorylation promotes transcriptional activity (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiQ04752.

Expressioni

Tissue specificityi

Adrenal, ovary, testis, placenta, adipocyte, and brain.

Interactioni

Subunit structurei

Binds DNA as a monomer. Part of a complex consisting of SFPQ, NONO and NR5A1. Interacts with NR0B2. Interacts with DGKQ and CDK7. Binds to and activated by HIPK3 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
EDF1O60869-14EBI-850837,EBI-781310From a different organism.

Protein-protein interaction databases

IntActiQ04752. 1 interaction.
STRINGi9913.ENSBTAP00000011869.

Structurei

3D structure databases

ProteinModelPortaliQ04752.
SMRiQ04752. Positions 10-111, 236-460.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni230 – 461232Important for dimerizationAdd
BLAST
Regioni260 – 34788Ligand-bindingBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri13 – 3321NR C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri49 – 7325NR C4-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG240365.
GeneTreeiENSGT00780000121906.
HOGENOMiHOG000063718.
HOVERGENiHBG106677.
InParanoidiQ04752.
KOiK08560.
OMAiLQEPAKG.
OrthoDBiEOG7BS4B9.
TreeFamiTF350737.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProiIPR016355. NR5_fam.
IPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001723. Str_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PANTHERiPTHR24086. PTHR24086. 1 hit.
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PIRSFiPIRSF002530. Nuc_orph_FTZ-F1. 1 hit.
PRINTSiPR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 2 hits.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04752-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYSYDEDLD ELCPVCGDKV SGYHYGLLTC ESCKGFFKRT VQNNKHYTCT
60 70 80 90 100
ESQSCKIDKT QRKRCPFCRF QKCLTVGMRL EAVRADRMRG GRNKFGPMYK
110 120 130 140 150
RDRALKQQKK AQIRANGFKL ETGPPVGVPP PPPPPPDYML PHGLHASEPK
160 170 180 190 200
GLASGPPAGP LGDFGAPALP MAVPSAHGPL AGYLYPAFPG RAIKSEYPEP
210 220 230 240 250
YASPPQPGPP YGYPEPFSGG PGVPELILQL LQLEPDEDQV RARIVGCLQE
260 270 280 290 300
PAKGRPDQPA PFSLLCRMAD QTFISIVDWA RRCMVFKELE VADQMTLLQN
310 320 330 340 350
CWSELLVFDH IYRQIQHGKE GSILLVTGQE VELTTVAAQA GSLLHSLVLR
360 370 380 390 400
AQELVLQLHA LQLDRQEFVC LKFLILFSLD VKFLNNHSLV KEAQEKANAA
410 420 430 440 450
LLDYTLCHYP HCGDKFQQLL LCLVEVRALS MQAKEYLYHK HLGNEMPRNN
460
LLIEMLQAKQ T
Length:461
Mass (Da):51,613
Last modified:October 1, 1993 - v1
Checksum:i910747C467ABBD6E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti64 – 652Missing in AAB23574 (PubMed:1406703).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13569 mRNA. Translation: BAA02764.1.
S45997 mRNA. Translation: AAB23574.2.
PIRiA46077.
RefSeqiNP_776828.1. NM_174403.2.
XP_010808627.1. XM_010810325.1.
UniGeneiBt.7422.

Genome annotation databases

EnsembliENSBTAT00000011869; ENSBTAP00000011869; ENSBTAG00000009017.
GeneIDi281948.
KEGGibta:281948.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13569 mRNA. Translation: BAA02764.1.
S45997 mRNA. Translation: AAB23574.2.
PIRiA46077.
RefSeqiNP_776828.1. NM_174403.2.
XP_010808627.1. XM_010810325.1.
UniGeneiBt.7422.

3D structure databases

ProteinModelPortaliQ04752.
SMRiQ04752. Positions 10-111, 236-460.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ04752. 1 interaction.
STRINGi9913.ENSBTAP00000011869.

Proteomic databases

PRIDEiQ04752.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000011869; ENSBTAP00000011869; ENSBTAG00000009017.
GeneIDi281948.
KEGGibta:281948.

Organism-specific databases

CTDi2516.

Phylogenomic databases

eggNOGiNOG240365.
GeneTreeiENSGT00780000121906.
HOGENOMiHOG000063718.
HOVERGENiHBG106677.
InParanoidiQ04752.
KOiK08560.
OMAiLQEPAKG.
OrthoDBiEOG7BS4B9.
TreeFamiTF350737.

Enzyme and pathway databases

ReactomeiREACT_276414. Nuclear Receptor transcription pathway.

Miscellaneous databases

NextBioi20805824.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProiIPR016355. NR5_fam.
IPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001723. Str_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PANTHERiPTHR24086. PTHR24086. 1 hit.
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PIRSFiPIRSF002530. Nuc_orph_FTZ-F1. 1 hit.
PRINTSiPR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 2 hits.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Ad4BP regulating steroidogenic P-450 gene is a member of steroid hormone receptor superfamily."
    Honda S., Morohashi K., Nomura M., Takeya H., Kitajima M., Omura T.
    J. Biol. Chem. 268:7494-7502(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 120-180.
    Tissue: Adrenal cortex.
  2. "Steroidogenic factor I, a key regulator of steroidogenic enzyme expression, is the mouse homolog of fushi tarazu-factor I."
    Lala D.S., Rice D.A., Parker K.L.
    Mol. Endocrinol. 6:1249-1258(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-81.

Entry informationi

Entry nameiSTF1_BOVIN
AccessioniPrimary (citable) accession number: Q04752
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: April 1, 2015
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.