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Q04752

- STF1_BOVIN

UniProt

Q04752 - STF1_BOVIN

Protein

Steroidogenic factor 1

Gene

NR5A1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Transcriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei341 – 3411Lipid headgroup; via amide nitrogenBy similarity
    Binding sitei436 – 4361Lipid headgroupBy similarity
    Binding sitei440 – 4401Lipid headgroupBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi10 – 8576Nuclear receptorPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri13 – 3321NR C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri49 – 7325NR C4-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: Ensembl
    2. DNA binding Source: HGNC
    3. phospholipid binding Source: Ensembl
    4. protein binding Source: UniProtKB
    5. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: Ensembl
    6. sequence-specific DNA binding Source: UniProtKB
    7. steroid hormone receptor activity Source: UniProtKB
    8. zinc ion binding Source: InterPro

    GO - Biological processi

    1. adrenal gland development Source: Ensembl
    2. cell differentiation Source: Ensembl
    3. hormone metabolic process Source: Ensembl
    4. luteinization Source: Ensembl
    5. maintenance of protein location in nucleus Source: Ensembl
    6. male gonad development Source: Ensembl
    7. multicellular organismal aging Source: Ensembl
    8. negative regulation of female gonad development Source: Ensembl
    9. positive regulation of male gonad development Source: Ensembl
    10. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
    11. tissue development Source: Ensembl

    Keywords - Molecular functioni

    Activator, Receptor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Lipid-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_210423. Nuclear Receptor transcription pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Steroidogenic factor 1
    Short name:
    SF-1
    Short name:
    STF-1
    Alternative name(s):
    Adrenal 4-binding protein
    Fushi tarazu factor homolog 1
    Nuclear receptor subfamily 5 group A member 1
    Steroid hormone receptor Ad4BP
    Gene namesi
    Name:NR5A1
    Synonyms:AD4BP, FTZF1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 11

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 461461Steroidogenic factor 1PRO_0000053728Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki119 – 119Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
    Cross-linki194 – 194Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
    Modified residuei203 – 2031Phosphoserine; by CDK7By similarity

    Post-translational modificationi

    May be regulated by phosphorylation and dephosphorylation.
    Acetylation stimulates the transcriptional activity.By similarity
    Sumoylation reduces CDK7-mediated phosophorylation on Ser-203.By similarity
    Phosphorylated on Ser-203 by CDK7. This phosphorylation promotes transcriptional activity By similarity.By similarity

    Keywords - PTMi

    Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiQ04752.

    Expressioni

    Tissue specificityi

    Adrenal, ovary, testis, placenta, adipocyte, and brain.

    Interactioni

    Subunit structurei

    Binds DNA as a monomer. Part of a complex consisting of SFPQ, NONO and NR5A1. Interacts with NR0B2. Interacts with DGKQ and CDK7. Binds to and activated by HIPK3 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    EDF1O60869-14EBI-850837,EBI-781310From a different organism.

    Protein-protein interaction databases

    IntActiQ04752. 1 interaction.
    STRINGi9913.ENSBTAP00000011869.

    Structurei

    3D structure databases

    ProteinModelPortaliQ04752.
    SMRiQ04752. Positions 10-111, 236-460.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni230 – 461232Important for dimerizationAdd
    BLAST
    Regioni260 – 34788Ligand-bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri13 – 3321NR C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri49 – 7325NR C4-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG240365.
    GeneTreeiENSGT00750000117458.
    HOGENOMiHOG000063718.
    HOVERGENiHBG106677.
    InParanoidiQ04752.
    KOiK08560.
    OMAiLQEPAKG.
    OrthoDBiEOG7BS4B9.
    TreeFamiTF350737.

    Family and domain databases

    Gene3Di1.10.565.10. 1 hit.
    3.30.50.10. 1 hit.
    InterProiIPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR016355. Steroidogenic_factor_1.
    IPR001723. Str_hrmn_rcpt.
    IPR001628. Znf_hrmn_rcpt.
    IPR013088. Znf_NHR/GATA.
    [Graphical view]
    PfamiPF00104. Hormone_recep. 1 hit.
    PF00105. zf-C4. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002530. Nuc_orph_FTZ-F1. 1 hit.
    PRINTSiPR00398. STRDHORMONER.
    PR00047. STROIDFINGER.
    SMARTiSM00430. HOLI. 1 hit.
    SM00399. ZnF_C4. 1 hit.
    [Graphical view]
    SUPFAMiSSF48508. SSF48508. 2 hits.
    PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
    PS51030. NUCLEAR_REC_DBD_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q04752-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYSYDEDLD ELCPVCGDKV SGYHYGLLTC ESCKGFFKRT VQNNKHYTCT    50
    ESQSCKIDKT QRKRCPFCRF QKCLTVGMRL EAVRADRMRG GRNKFGPMYK 100
    RDRALKQQKK AQIRANGFKL ETGPPVGVPP PPPPPPDYML PHGLHASEPK 150
    GLASGPPAGP LGDFGAPALP MAVPSAHGPL AGYLYPAFPG RAIKSEYPEP 200
    YASPPQPGPP YGYPEPFSGG PGVPELILQL LQLEPDEDQV RARIVGCLQE 250
    PAKGRPDQPA PFSLLCRMAD QTFISIVDWA RRCMVFKELE VADQMTLLQN 300
    CWSELLVFDH IYRQIQHGKE GSILLVTGQE VELTTVAAQA GSLLHSLVLR 350
    AQELVLQLHA LQLDRQEFVC LKFLILFSLD VKFLNNHSLV KEAQEKANAA 400
    LLDYTLCHYP HCGDKFQQLL LCLVEVRALS MQAKEYLYHK HLGNEMPRNN 450
    LLIEMLQAKQ T 461
    Length:461
    Mass (Da):51,613
    Last modified:October 1, 1993 - v1
    Checksum:i910747C467ABBD6E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti64 – 652Missing in AAB23574. (PubMed:1406703)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13569 mRNA. Translation: BAA02764.1.
    S45997 mRNA. Translation: AAB23574.2.
    PIRiA46077.
    RefSeqiNP_776828.1. NM_174403.2.
    UniGeneiBt.7422.

    Genome annotation databases

    EnsembliENSBTAT00000011869; ENSBTAP00000011869; ENSBTAG00000009017.
    GeneIDi281948.
    KEGGibta:281948.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13569 mRNA. Translation: BAA02764.1 .
    S45997 mRNA. Translation: AAB23574.2 .
    PIRi A46077.
    RefSeqi NP_776828.1. NM_174403.2.
    UniGenei Bt.7422.

    3D structure databases

    ProteinModelPortali Q04752.
    SMRi Q04752. Positions 10-111, 236-460.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q04752. 1 interaction.
    STRINGi 9913.ENSBTAP00000011869.

    Proteomic databases

    PRIDEi Q04752.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000011869 ; ENSBTAP00000011869 ; ENSBTAG00000009017 .
    GeneIDi 281948.
    KEGGi bta:281948.

    Organism-specific databases

    CTDi 2516.

    Phylogenomic databases

    eggNOGi NOG240365.
    GeneTreei ENSGT00750000117458.
    HOGENOMi HOG000063718.
    HOVERGENi HBG106677.
    InParanoidi Q04752.
    KOi K08560.
    OMAi LQEPAKG.
    OrthoDBi EOG7BS4B9.
    TreeFami TF350737.

    Enzyme and pathway databases

    Reactomei REACT_210423. Nuclear Receptor transcription pathway.

    Miscellaneous databases

    NextBioi 20805824.

    Family and domain databases

    Gene3Di 1.10.565.10. 1 hit.
    3.30.50.10. 1 hit.
    InterProi IPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR016355. Steroidogenic_factor_1.
    IPR001723. Str_hrmn_rcpt.
    IPR001628. Znf_hrmn_rcpt.
    IPR013088. Znf_NHR/GATA.
    [Graphical view ]
    Pfami PF00104. Hormone_recep. 1 hit.
    PF00105. zf-C4. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002530. Nuc_orph_FTZ-F1. 1 hit.
    PRINTSi PR00398. STRDHORMONER.
    PR00047. STROIDFINGER.
    SMARTi SM00430. HOLI. 1 hit.
    SM00399. ZnF_C4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48508. SSF48508. 2 hits.
    PROSITEi PS00031. NUCLEAR_REC_DBD_1. 1 hit.
    PS51030. NUCLEAR_REC_DBD_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Ad4BP regulating steroidogenic P-450 gene is a member of steroid hormone receptor superfamily."
      Honda S., Morohashi K., Nomura M., Takeya H., Kitajima M., Omura T.
      J. Biol. Chem. 268:7494-7502(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 120-180.
      Tissue: Adrenal cortex.
    2. "Steroidogenic factor I, a key regulator of steroidogenic enzyme expression, is the mouse homolog of fushi tarazu-factor I."
      Lala D.S., Rice D.A., Parker K.L.
      Mol. Endocrinol. 6:1249-1258(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-81.

    Entry informationi

    Entry nameiSTF1_BOVIN
    AccessioniPrimary (citable) accession number: Q04752
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3