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Reviewed, UniProtKB/Swiss-Prot Q04750 (TOP1_MOUSE)

Last modified February 9, 2010. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    DNA topoisomerase 1
    EC=5.99.1.2
Alternative name(s):
    DNA topoisomerase I
Gene names
Name: Top1
Synonyms: Top-1
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMus

Protein attributes

Sequence length767 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Subunit structure

Monomer.

Subcellular location

Nucleusnucleolus. Nucleusnucleoplasm. Note: Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli By similarity.

Post-translational modification

Sumoylated. Lys-119 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment By similarity.

Miscellaneous

Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.

When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.

Sequence similarities

Belongs to the eukaryotic type I topoisomerase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 767766DNA topoisomerase 1
PRO_0000145202

Regions

Compositional bias23 – 206184Lys-rich

Sites

Active site7251O-(3'-phospho-DNA)-tyrosine intermediate By similarity

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue1141Phosphoserine By similarity
Modified residue2821N6-acetyllysine By similarity
Cross-link105Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) Probable
Cross-link119Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Cross-link155Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) Probable

Experimental info

Sequence conflict911R → P in AAA40466. Ref.2
Sequence conflict1211E → D in BAA00950. Ref.1
Sequence conflict1291A → V in AAA40466. Ref.2
Sequence conflict1611Missing in AAA40466. Ref.2
Sequence conflict1671S → L in AAA40466. Ref.2
Sequence conflict2771R → W in AAA40466. Ref.2
Sequence conflict2921E → G in BAA00950. Ref.1
Sequence conflict5221G → V in AAA40466. Ref.2
Sequence conflict5331G → W in AAA40466. Ref.2
Sequence conflict7621D → Y in AAA40466. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q04750-1 [UniParc].

Last modified July 24, 2007. Version 2.
Checksum: 4EA654244F07D5C1

FASTA76790,876
        10         20         30         40         50         60 
MSGDHLHNDS QIEADFRLND SHKHKDKHKD REHRHKEHKK DKDKDREKSK HSNSEHKDSE 

        70         80         90        100        110        120 
KKHKEKEKTK HKDGSSEKHK DKHKDRDKER RKEEKIRAAG DAKIKKEKEN GFSSPPRIKD 

       130        140        150        160        170        180 
EPEDDGYFAP PKEDIKPLKR LRDEDDADYK PKKIKTEDIK KEKKRKSEEE EDGKLKKPKN 

       190        200        210        220        230        240 
KDKDKKVAEP DNKKKKPKKE EEQKWKWWEE ERYPEGIKWK FLEHKGPVFA PPYEPLPESV 

       250        260        270        280        290        300 
KFYYDGKVMK LSPKAEEVAT FFAKMLDHEY TTKEIFRKNF FKDWRKEMTN DEKNTITNLS 

       310        320        330        340        350        360 
KCDFTQMSQY FKAQSEARKQ MSKEEKLKIK EENEKLLKEY GFCVMDNHRE RIANFKIEPP 

       370        380        390        400        410        420 
GLFRGRGNHP KMGMLKRRIM PEDIIINCSK DAKVPSPPPG HKWKEVRHDN KVTWLVSWTE 

       430        440        450        460        470        480 
NIQGSIKYIM LNPSSRIKGE KDWQKYETAR RLKKCVDKIR NQYREDWKSK EMKVRQRAVA 

       490        500        510        520        530        540 
LYFIDKLALR AGNEKEEGET ADTVGCCSLR VEHINLHPEL DGQEYVVEFD FPGKDSIRYY 

       550        560        570        580        590        600 
NKVPVEKRVF KNLQLFMENK QPEDDLFDRL NTGILNKHLQ DLMEGLTAKV FRTYNASITL 

       610        620        630        640        650        660 
QQQLKELTAP DENVPAKILS YNRANRAVAI LCNHQRAPPK TFEKSMMNLQ SKIDAKKDQL 

       670        680        690        700        710        720 
ADARRDLKSA KADAKVMKDA KTKKVVESKK KAVQRLEEQL MKLEVQATDR EENKQIALGT 

       730        740        750        760 
SKLNYLDPRI TVAWCKKWGV PIEKIYNKTQ REKFAWAIDM TDEDYEF 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of the mouse cDNA encoding DNA topoisomerase I and chromosomal location of the gene."
Koiwai O., Yasui Y., Sakai Y., Watanabe T., Ishii K., Yanagihara S., Andoh T.
Gene 125:211-216(1993) [PubMed: 8096488] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]"Cloning and characterization of mouse topoisomerase I cDNA."
Hui C.-F., Lo C.K., Hwang J.
Submitted (JUL-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D10061 mRNA. Translation: BAA00950.1.
L20632 mRNA. Translation: AAA40466.1.
AL590414, AL591673 Genomic DNA. Translation: CAM20297.1.
AL591673, AL590414 Genomic DNA. Translation: CAM25349.1.
IPIIPI00109764.
PIRJU0144.
RefSeqNP_033434.2.
UniGeneMm.217233

3D structure databases

SMRQ04750. Positions 201-767.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ04750.

PTM databases

PhosphoSiteQ04750.

Proteomic databases

PRIDEQ04750.

Genome annotation databases

EnsemblENSMUST00000109468; ENSMUSP00000105094; ENSMUSG00000070544; Mus musculus. [Genome view]
GeneID21969.
KEGGmmu:21969.
UCSCuc008nqy.1. mouse.

Organism-specific databases

CTD21969.
MGIMGI:98788. Top1.

Phylogenomic databases

eggNOGroNOG09781.
HOGENOMHBG521929.
HOVERGENQ04750.
InParanoidQ04750.
OMAESVKFYY.
OrthoDBEOG9909W9.
PhylomeDBQ04750.

Enzyme and pathway databases

BRENDA5.99.1.2. 244.

Gene expression databases

ArrayExpressQ04750.
BgeeQ04750.
CleanExMM_TOP1.
GenevestigatorQ04750.
GermOnlineENSMUSG00000070544. Mus musculus.

Family and domain databases

InterProIPR011010. DNA_brk_join_enz.
IPR018521. TopoI_AS.
IPR001631. TopoI_C.
IPR013499. TopoI_C_euk.
IPR008336. TopoI_DNA_bd_euk.
IPR009054. TopoI_insert_euk.
[Graphical view]
PfamPF02919. Topoisom_I_N. 1 hit.
[Graphical view]
PRINTSPR00416. EUTPISMRASEI.
SMARTSM00435. TOPEUc. 1 hit.
[Graphical view]
PROSITEPS00176. TOPOISOMERASE_I_EUK. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio301666.
PMAP-CutDBQ04750.
SOURCESearch...

Entry information

Entry nameTOP1_MOUSE
AccessionPrimary (citable) accession number: Q04750
Secondary accession number(s): A2A4B7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: July 24, 2007
Last modified: February 9, 2010
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents