Reviewed, UniProtKB/Swiss-Prot Q04750 (TOP1_MOUSE)

Names and origin

Protein names

Recommended name:
    DNA topoisomerase 1
    EC=5.99.1.2
Alternative name(s):
    DNA topoisomerase I

Gene names

Name:	Top1
Synonyms:	Top-1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	767 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Subunit structure	Monomer.
Subcellular location	Nucleus › nucleolus. Nucleus › nucleoplasm. Note: Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli By similarity.
Post-translational modification	Sumoylated. Lys-119 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment By similarity.
Miscellaneous	Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils. When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.
Sequence similarities	Belongs to the eukaryotic type I topoisomerase family.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
PTM	Acetylation Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	DNA replication Inferred from mutant phenotype. Source: MGI DNA topological change Inferred from direct assay. Source: MGI embryonic cleavage Inferred from mutant phenotype. Source: MGI
Cellular component	cytoplasm Inferred from direct assay. Source: MGI nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell nucleoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell perikaryon Inferred from direct assay. Source: MGI
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro DNA topoisomerase type I activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 767	766	DNA topoisomerase 1		PRO_0000145202
Regions
	Compositional bias	23 – 206	184	Lys-rich
Sites
	Active site	725	1	O-(3'-phospho-DNA)-tyrosine intermediate By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	114	1	Phosphoserine By similarity
	Modified residue	282	1	N6-acetyllysine By similarity
	Cross-link	105		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) Probable
	Cross-link	119		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
	Cross-link	155		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) Probable
Experimental info
	Sequence conflict	91	1	R → P in AAA40466. Ref.2
	Sequence conflict	121	1	E → D in BAA00950. Ref.1
	Sequence conflict	129	1	A → V in AAA40466. Ref.2
	Sequence conflict	161	1	Missing in AAA40466. Ref.2
	Sequence conflict	167	1	S → L in AAA40466. Ref.2
	Sequence conflict	277	1	R → W in AAA40466. Ref.2
	Sequence conflict	292	1	E → G in BAA00950. Ref.1
	Sequence conflict	522	1	G → V in AAA40466. Ref.2
	Sequence conflict	533	1	G → W in AAA40466. Ref.2
	Sequence conflict	762	1	D → Y in AAA40466. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q04750-1 [UniParc]. Last modified July 24, 2007. Version 2. Checksum: 4EA654244F07D5C1	FASTA	767	90,876
	10 20 30 40 50 60 MSGDHLHNDS QIEADFRLND SHKHKDKHKD REHRHKEHKK DKDKDREKSK HSNSEHKDSE 70 80 90 100 110 120 KKHKEKEKTK HKDGSSEKHK DKHKDRDKER RKEEKIRAAG DAKIKKEKEN GFSSPPRIKD 130 140 150 160 170 180 EPEDDGYFAP PKEDIKPLKR LRDEDDADYK PKKIKTEDIK KEKKRKSEEE EDGKLKKPKN 190 200 210 220 230 240 KDKDKKVAEP DNKKKKPKKE EEQKWKWWEE ERYPEGIKWK FLEHKGPVFA PPYEPLPESV 250 260 270 280 290 300 KFYYDGKVMK LSPKAEEVAT FFAKMLDHEY TTKEIFRKNF FKDWRKEMTN DEKNTITNLS 310 320 330 340 350 360 KCDFTQMSQY FKAQSEARKQ MSKEEKLKIK EENEKLLKEY GFCVMDNHRE RIANFKIEPP 370 380 390 400 410 420 GLFRGRGNHP KMGMLKRRIM PEDIIINCSK DAKVPSPPPG HKWKEVRHDN KVTWLVSWTE 430 440 450 460 470 480 NIQGSIKYIM LNPSSRIKGE KDWQKYETAR RLKKCVDKIR NQYREDWKSK EMKVRQRAVA 490 500 510 520 530 540 LYFIDKLALR AGNEKEEGET ADTVGCCSLR VEHINLHPEL DGQEYVVEFD FPGKDSIRYY 550 560 570 580 590 600 NKVPVEKRVF KNLQLFMENK QPEDDLFDRL NTGILNKHLQ DLMEGLTAKV FRTYNASITL 610 620 630 640 650 660 QQQLKELTAP DENVPAKILS YNRANRAVAI LCNHQRAPPK TFEKSMMNLQ SKIDAKKDQL 670 680 690 700 710 720 ADARRDLKSA KADAKVMKDA KTKKVVESKK KAVQRLEEQL MKLEVQATDR EENKQIALGT 730 740 750 760 SKLNYLDPRI TVAWCKKWGV PIEKIYNKTQ REKFAWAIDM TDEDYEF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of the mouse cDNA encoding DNA topoisomerase I and chromosomal location of the gene." Koiwai O., Yasui Y., Sakai Y., Watanabe T., Ishii K., Yanagihara S., Andoh T. Gene 125:211-216(1993) [PubMed: 8096488] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]	"Cloning and characterization of mouse topoisomerase I cDNA." Hui C.-F., Lo C.K., Hwang J. Submitted (JUL-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10061 mRNA. Translation: BAA00950.1. L20632 mRNA. Translation: AAA40466.1. AL590414, AL591673 Genomic DNA. Translation: CAM20297.1. AL591673, AL590414 Genomic DNA. Translation: CAM25349.1.
IPI	IPI00109764.
PIR	JU0144.
RefSeq	NP_033434.2.
UniGene	Mm.217233
3D structure databases
SMR	Q04750. Positions 201-767.
ModBase	Search...
Protein-protein interaction databases
STRING	Q04750.
PTM databases
PhosphoSite	Q04750.
Proteomic databases
PRIDE	Q04750.
Genome annotation databases
Ensembl	ENSMUST00000109468; ENSMUSP00000105094; ENSMUSG00000070544; Mus musculus. [Genome view]
GeneID	21969.
KEGG	mmu:21969.
UCSC	uc008nqy.1. mouse.
Organism-specific databases
CTD	21969.
MGI	MGI:98788. Top1.
Phylogenomic databases
eggNOG	roNOG09781.
HOGENOM	HBG521929.
HOVERGEN	Q04750.
InParanoid	Q04750.
OMA	ESVKFYY.
OrthoDB	EOG9909W9.
PhylomeDB	Q04750.
Enzyme and pathway databases
BRENDA	5.99.1.2. 244.
Gene expression databases
ArrayExpress	Q04750.
Bgee	Q04750.
CleanEx	MM_TOP1.
Genevestigator	Q04750.
GermOnline	ENSMUSG00000070544. Mus musculus.
Family and domain databases
InterPro	IPR011010. DNA_brk_join_enz. IPR018521. TopoI_AS. IPR001631. TopoI_C. IPR013499. TopoI_C_euk. IPR008336. TopoI_DNA_bd_euk. IPR009054. TopoI_insert_euk. [Graphical view]
Pfam	PF02919. Topoisom_I_N. 1 hit. [Graphical view]
PRINTS	PR00416. EUTPISMRASEI.
SMART	SM00435. TOPEUc. 1 hit. [Graphical view]
PROSITE	PS00176. TOPOISOMERASE_I_EUK. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	301666.
PMAP-CutDB	Q04750.
SOURCE	Search...

Entry information

Entry name

TOP1_MOUSE

Accession

Primary (citable) accession number: Q04750
Secondary accession number(s): A2A4B7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	July 24, 2007
Last modified:	February 9, 2010
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)