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Protein

Target of rapamycin complex 2 subunit AVO2

Gene

AVO2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of TORC2, which regulates cell cycle-dependent polarization of the actin-cytoskeleton and cell wall integrity. TORC2 controls polarity of the actin cytoskeleton, which is required for orienting the secretory pathway toward discrete growth sites, via the RHO1/PKC1/MAPK cell integrity pathway.

GO - Biological processi

  • establishment or maintenance of actin cytoskeleton polarity Source: SGD
  • fungal-type cell wall organization Source: SGD
  • regulation of cell growth Source: SGD
  • TOR signaling Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-32770-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Target of rapamycin complex 2 subunit AVO2
Short name:
TORC2 subunit AVO2
Alternative name(s):
Adheres voraciously to TOR2 protein 2
Gene namesi
Name:AVO2
Ordered Locus Names:YMR068W
ORF Names:YM9916.07
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR068W.
SGDiS000004672. AVO2.

Subcellular locationi

GO - Cellular componenti

  • plasma membrane Source: UniProtKB-SubCell
  • TORC2 complex Source: SGD
  • vacuolar membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 426426Target of rapamycin complex 2 subunit AVO2PRO_0000067247Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei315 – 3151PhosphoserineCombined sources
Modified residuei350 – 3501PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

PTM databases

iPTMnetiQ04749.

Interactioni

Subunit structurei

The target of rapamycin complex 2 (TORC2) is composed of at least AVO1, AVO2, BIT61, LST8, TOR2 and TSC11. TORC2 likely forms a homodimer. Contrary to TORC1, TORC2 does not bind to and is not sensitive to FKBP-rapamycin. AVO2 is peripherally associated to AVO1 and TSC11.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
LST8P413182EBI-28131,EBI-28598
TOR2P326004EBI-28131,EBI-19385

Protein-protein interaction databases

BioGridi35243. 51 interactions.
DIPiDIP-1958N.
IntActiQ04749. 10 interactions.
MINTiMINT-392081.

Structurei

3D structure databases

ProteinModelPortaliQ04749.
SMRiQ04749. Positions 8-253.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati4 – 3330ANK 1Add
BLAST
Repeati39 – 6830ANK 2Add
BLAST
Repeati74 – 10431ANK 3Add
BLAST
Repeati108 – 13730ANK 4Add
BLAST
Repeati141 – 17131ANK 5Add
BLAST

Sequence similaritiesi

Contains 5 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

GeneTreeiENSGT00840000131053.
HOGENOMiHOG000034239.
InParanoidiQ04749.
KOiK06867.
OMAiHGRYLIC.
OrthoDBiEOG73BVNP.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF00023. Ank. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 4 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04749-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKEPSVRLR EAIIEGNLLI VKRLLRRNPD LLTNIDSENG WSSLHYASYH
60 70 80 90 100
GRYLICVYLI QLGHDKHELI KTFKGNTCVH LALMKGHEQT LHLLLQQFPR
110 120 130 140 150
FINHRGENGR APIHIACMND YYQCLSLLIG VGADLWVMDT NGDTPLHVCL
160 170 180 190 200
EYGSISCMKM LLNEGEVSLD DNVRDKGNWK PIDVAQTFEV GNIYSKVLKE
210 220 230 240 250
VKKKGPPLGA GKKPSSFRTP ILNAKATFED GPSPVLSMNS PYSLYSNNSP
260 270 280 290 300
LPVLPRRIST HTTSGNGGNR RSSITNPVFN PRKPTLSTDS FSSSSNSSSR
310 320 330 340 350
LRVNSINVKT PVGVSPKKEL VSESVRHSAT PTSPHNNIAL INRYLLPNKS
360 370 380 390 400
NDNVRGDSQT ATINDDGGGG NGGDATIGMG LRKDPDDENE NKYKIKVNNG
410 420
EPRRRVSLLN IPISKLRNSN NTRAED
Length:426
Mass (Da):47,139
Last modified:November 1, 1997 - v1
Checksum:i0A17E78BF449CBFE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48952 Genomic DNA. Translation: CAA88793.1.
BK006946 Genomic DNA. Translation: DAA09966.1.
PIRiS52828.
RefSeqiNP_013784.1. NM_001182566.1.

Genome annotation databases

EnsemblFungiiYMR068W; YMR068W; YMR068W.
GeneIDi855090.
KEGGisce:YMR068W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48952 Genomic DNA. Translation: CAA88793.1.
BK006946 Genomic DNA. Translation: DAA09966.1.
PIRiS52828.
RefSeqiNP_013784.1. NM_001182566.1.

3D structure databases

ProteinModelPortaliQ04749.
SMRiQ04749. Positions 8-253.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35243. 51 interactions.
DIPiDIP-1958N.
IntActiQ04749. 10 interactions.
MINTiMINT-392081.

PTM databases

iPTMnetiQ04749.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR068W; YMR068W; YMR068W.
GeneIDi855090.
KEGGisce:YMR068W.

Organism-specific databases

EuPathDBiFungiDB:YMR068W.
SGDiS000004672. AVO2.

Phylogenomic databases

GeneTreeiENSGT00840000131053.
HOGENOMiHOG000034239.
InParanoidiQ04749.
KOiK06867.
OMAiHGRYLIC.
OrthoDBiEOG73BVNP.

Enzyme and pathway databases

BioCyciYEAST:G3O-32770-MONOMER.

Miscellaneous databases

NextBioi978393.
PROiQ04749.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF00023. Ank. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 4 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control."
    Loewith R., Jacinto E., Wullschleger S., Lorberg A., Crespo J.L., Bonenfant D., Oppliger W., Jenoe P., Hall M.N.
    Mol. Cell 10:457-468(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN TORC2, IDENTIFICATION BY MASS SPECTROMETRY.
  4. "Tor kinases are in distinct membrane-associated protein complexes in Saccharomyces cerevisiae."
    Wedaman K.P., Reinke A., Anderson S., Yates J.R. III, McCaffery J.M., Powers T.
    Mol. Biol. Cell 14:1204-1220(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN TORC2, IDENTIFICATION BY MASS SPECTROMETRY.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "The pleckstrin homology domain proteins Slm1 and Slm2 are required for actin cytoskeleton organization in yeast and bind phosphatidylinositol-4,5-bisphosphate and TORC2."
    Fadri M., Daquinag A., Wang S., Xue T., Kunz J.
    Mol. Biol. Cell 16:1883-1900(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SLM1 AND SLM2.
  7. "Molecular organization of target of rapamycin complex 2."
    Wullschleger S., Loewith R., Oppliger W., Hall M.N.
    J. Biol. Chem. 280:30697-30704(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-350, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-315, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAVO2_YEAST
AccessioniPrimary (citable) accession number: Q04749
Secondary accession number(s): D6VZP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 11, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1180 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.