ID   EMX2_MOUSE              Reviewed;         253 AA.
AC   Q04744; G3X9A6; Q80UE7;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2012, sequence version 3.
DT   24-JAN-2024, entry version 169.
DE   RecName: Full=Homeobox protein EMX2;
DE   AltName: Full=Empty spiracles homolog 2;
DE   AltName: Full=Empty spiracles-like protein 2;
GN   Name=Emx2; Synonyms=Emx-2;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=FVB/N;
RX   PubMed=12573261; DOI=10.1016/s0888-7543(02)00023-x;
RA   Noonan F.C., Goodfellow P.J., Staloch L.J., Mutch D.G., Simon T.C.;
RT   "Antisense transcripts at the EMX2 locus in human and mouse.";
RL   Genomics 81:58-66(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 155-220, FUNCTION, AND TISSUE SPECIFICITY.
RX   PubMed=1352754; DOI=10.1002/j.1460-2075.1992.tb05319.x;
RA   Simeone A., Gulisano M., Acampora D., Stornaiuolo A., Rambaldi M.,
RA   Boncinelli E.;
RT   "Two vertebrate homeobox genes related to the Drosophila empty spiracles
RT   gene are expressed in the embryonic cerebral cortex.";
RL   EMBO J. 11:2541-2550(1992).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 155-220.
RC   TISSUE=Brain;
RX   PubMed=1353865; DOI=10.1038/358687a0;
RA   Simeone A., Acampora D., Gulisano M., Stornaiuolo A., Boncinelli E.;
RT   "Nested expression domains of four homeobox genes in developing rostral
RT   brain.";
RL   Nature 358:687-690(1992).
RN   [6]
RP   FUNCTION.
RX   PubMed=15147765; DOI=10.1016/j.mod.2004.03.013;
RA   Shinozaki K., Yoshida M., Nakamura M., Aizawa S., Suda Y.;
RT   "Emx1 and Emx2 cooperate in initial phase of archipallium development.";
RL   Mech. Dev. 121:475-489(2004).
RN   [7]
RP   INTERACTION WITH EIF4E, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX   PubMed=15247416; DOI=10.1073/pnas.0403824101;
RA   Nedelec S., Foucher I., Brunet I., Bouillot C., Prochiantz A.,
RA   Trembleau A.;
RT   "Emx2 homeodomain transcription factor interacts with eukaryotic
RT   translation initiation factor 4E (eIF4E) in the axons of olfactory sensory
RT   neurons.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:10815-10820(2004).
RN   [8]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Kidney;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Transcription factor, which in cooperation with EMX1, acts to
CC       generate the boundary between the roof and archipallium in the
CC       developing brain. May function in combination with OTX1/2 to specify
CC       cell fates in the developing central nervous system.
CC       {ECO:0000269|PubMed:1352754, ECO:0000269|PubMed:15147765}.
CC   -!- SUBUNIT: Interacts with translation initiation factor EIF4E.
CC       {ECO:0000269|PubMed:15247416}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15247416}. Cell
CC       projection, axon {ECO:0000269|PubMed:15247416}. Note=Detected in axons
CC       within the olfactory mucosa and glomeruli in the olfactory bulb.
CC       {ECO:0000269|PubMed:15247416}.
CC   -!- TISSUE SPECIFICITY: Expressed in immature and mature olfactory sensory
CC       neurons (at protein level) (PubMed:15247416). Cerebral cortex
CC       (PubMed:1352754). {ECO:0000269|PubMed:1352754,
CC       ECO:0000269|PubMed:15247416}.
CC   -!- SIMILARITY: Belongs to the EMX homeobox family. {ECO:0000305}.
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DR   EMBL; AY117415; AAM94018.1; -; mRNA.
DR   EMBL; AC098733; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH466585; EDL01826.1; -; Genomic_DNA.
DR   EMBL; X68882; CAA48753.1; -; mRNA.
DR   CCDS; CCDS29937.1; -.
DR   PIR; S29086; S29086.
DR   RefSeq; NP_034262.2; NM_010132.2.
DR   AlphaFoldDB; Q04744; -.
DR   SMR; Q04744; -.
DR   BioGRID; 199443; 4.
DR   IntAct; Q04744; 4.
DR   STRING; 10090.ENSMUSP00000053361; -.
DR   iPTMnet; Q04744; -.
DR   PhosphoSitePlus; Q04744; -.
DR   PaxDb; 10090-ENSMUSP00000053361; -.
DR   PeptideAtlas; Q04744; -.
DR   ProteomicsDB; 275750; -.
DR   Antibodypedia; 18764; 353 antibodies from 32 providers.
DR   DNASU; 13797; -.
DR   Ensembl; ENSMUST00000062216.4; ENSMUSP00000053361.3; ENSMUSG00000043969.5.
DR   GeneID; 13797; -.
DR   KEGG; mmu:13797; -.
DR   UCSC; uc008ibl.1; mouse.
DR   AGR; MGI:95388; -.
DR   CTD; 2018; -.
DR   MGI; MGI:95388; Emx2.
DR   VEuPathDB; HostDB:ENSMUSG00000043969; -.
DR   eggNOG; KOG0843; Eukaryota.
DR   GeneTree; ENSGT00940000157425; -.
DR   HOGENOM; CLU_049668_1_0_1; -.
DR   InParanoid; Q04744; -.
DR   OMA; RWRMETQ; -.
DR   OrthoDB; 903523at2759; -.
DR   PhylomeDB; Q04744; -.
DR   TreeFam; TF317015; -.
DR   BioGRID-ORCS; 13797; 0 hits in 77 CRISPR screens.
DR   ChiTaRS; Emx2; mouse.
DR   PRO; PR:Q04744; -.
DR   Proteomes; UP000000589; Chromosome 19.
DR   RNAct; Q04744; Protein.
DR   Bgee; ENSMUSG00000043969; Expressed in fallopian tube and 185 other cell types or tissues.
DR   ExpressionAtlas; Q04744; baseline and differential.
DR   GO; GO:0030424; C:axon; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR   GO; GO:0009952; P:anterior/posterior pattern specification; IMP:MGI.
DR   GO; GO:0007420; P:brain development; IMP:MGI.
DR   GO; GO:0021846; P:cell proliferation in forebrain; IMP:MGI.
DR   GO; GO:0007417; P:central nervous system development; IBA:GO_Central.
DR   GO; GO:0021987; P:cerebral cortex development; IGI:MGI.
DR   GO; GO:0021796; P:cerebral cortex regionalization; IMP:MGI.
DR   GO; GO:0021542; P:dentate gyrus development; IMP:MGI.
DR   GO; GO:0021885; P:forebrain cell migration; IMP:MGI.
DR   GO; GO:0030900; P:forebrain development; IMP:MGI.
DR   GO; GO:0030182; P:neuron differentiation; IMP:MGI.
DR   GO; GO:0001764; P:neuron migration; IMP:MGI.
DR   GO; GO:0010468; P:regulation of gene expression; IMP:MGI.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0009410; P:response to xenobiotic stimulus; IDA:MGI.
DR   GO; GO:0072197; P:ureter morphogenesis; IGI:MGI.
DR   CDD; cd00086; homeodomain; 1.
DR   Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR020479; Homeobox_metazoa.
DR   InterPro; IPR000047; HTH_motif.
DR   PANTHER; PTHR24339; HOMEOBOX PROTEIN EMX-RELATED; 1.
DR   PANTHER; PTHR24339:SF25; HOMEOBOX PROTEIN EMX2; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   PRINTS; PR00024; HOMEOBOX.
DR   PRINTS; PR00031; HTHREPRESSR.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; Homeodomain-like; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   Genevisible; Q04744; MM.
PE   1: Evidence at protein level;
KW   Cell projection; Developmental protein; DNA-binding; Homeobox; Nucleus;
KW   Reference proteome.
FT   CHAIN           1..253
FT                   /note="Homeobox protein EMX2"
FT                   /id="PRO_0000048869"
FT   DNA_BIND        155..214
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          213..253
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        213..228
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        103
FT                   /note="S -> L (in Ref. 1; AAM94018)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   253 AA;  28374 MW;  4AE978446786B204 CRC64;
     MFQPAPKRCF TIESLVAKDS PLPASRSEDP IRPAALSYAN SSPINPFLNG FHSAAAAAAA
     GRGVYSNPDL VFAEAVSHPP NPAVPVHPVP PPHALAAHPL PSSHSPHPLF ASQQRDPSTF
     YPWLIHRYRY LGHRFQGNDT SPESFLLHNA LARKPKRIRT AFSPSQLLRL EHAFEKNHYV
     VGAERKQLAH SLSLTETQVK VWFQNRRTKF KRQKLEEEGS DSQQKKKGTH HINRWRIATK
     QASPEEIDVT SDD
//