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Protein

Ribonuclease H

Gene

RNH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.

Miscellaneous

Present with 149 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.PROSITE-ProRule annotation

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi193Magnesium 1PROSITE-ProRule annotation1
Metal bindingi193Magnesium 2PROSITE-ProRule annotation1
Metal bindingi235Magnesium 1PROSITE-ProRule annotation1
Metal bindingi264Magnesium 1PROSITE-ProRule annotation1
Metal bindingi338Magnesium 2PROSITE-ProRule annotation1

GO - Molecular functioni

  • magnesium ion binding Source: InterPro
  • nucleic acid binding Source: InterPro
  • RNA-DNA hybrid ribonuclease activity Source: SGD

GO - Biological processi

  • DNA replication, removal of RNA primer Source: GO_Central
  • RNA catabolic process Source: SGD

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32915-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease H (EC:3.1.26.4)
Short name:
RNase H
Gene namesi
Name:RNH1
Ordered Locus Names:YMR234W
ORF Names:YM9959.16
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR234W
SGDiS000004847 RNH1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001954381 – 348Ribonuclease HAdd BLAST348

Proteomic databases

MaxQBiQ04740
PaxDbiQ04740
PRIDEiQ04740

Interactioni

Protein-protein interaction databases

BioGridi35412, 101 interactors
DIPiDIP-6787N
IntActiQ04740, 5 interactors
STRINGi4932.YMR234W

Structurei

Secondary structure

1348
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 10Combined sources5
Beta strandi12 – 15Combined sources4
Beta strandi17 – 21Combined sources5
Helixi22 – 28Combined sources7
Beta strandi29 – 31Combined sources3
Beta strandi38 – 41Combined sources4
Helixi43 – 50Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QHKNMR-A5-51[»]
ProteinModelPortaliQ04740
SMRiQ04740
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ04740

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini184 – 346RNase HPROSITE-ProRule annotationAdd BLAST163

Sequence similaritiesi

Belongs to the RNase H family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000003466
HOGENOMiHOG000066052
InParanoidiQ04740
KOiK03469
OMAiELWYGLY
OrthoDBiEOG092C42S8

Family and domain databases

Gene3Di3.30.420.10, 1 hit
3.40.970.10, 2 hits
InterProiView protein in InterPro
IPR009027 Ribosomal_L9/RNase_H1_N
IPR017067 RNase_H1_euk
IPR011320 RNase_H1_N
IPR037056 RNase_H1_N_sf
IPR012337 RNaseH-like_sf
IPR002156 RNaseH_domain
IPR036397 RNaseH_sf
PfamiView protein in Pfam
PF01693 Cauli_VI, 2 hits
PF00075 RNase_H, 1 hit
PIRSFiPIRSF036852 Ribonuclease_H1_euk, 1 hit
SUPFAMiSSF53098 SSF53098, 1 hit
SSF55658 SSF55658, 2 hits
PROSITEiView protein in PROSITE
PS50879 RNASE_H, 1 hit

Sequencei

Sequence statusi: Complete.

Q04740-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARQGNFYAV RKGRETGIYN TWNECKNQVD GYGGAIYKKF NSYEQAKSFL
60 70 80 90 100
GQPNTTSNYG SSTHAGGQVS KPHTTQKRVH RRNRPLHYSS LTSSSACSSL
110 120 130 140 150
SSANTNTFYS VKSNVPNIES KIFNNWKDCQ AYVKHKRGIT FKKFEDQLAA
160 170 180 190 200
ENFISGMSAH DYKLMNISKE SFESKYKLSS NTMYNKSMNV YCDGSSFGNG
210 220 230 240 250
TSSSRAGYGA YFEGAPEENI SEPLLSGAQT NNRAEIEAVS EALKKIWEKL
260 270 280 290 300
TNEKEKVNYQ IKTDSEYVTK LLNDRYMTYD NKKLEGLPNS DLIVPLVQRF
310 320 330 340
VKVKKYYELN KECFKNNGKF QIEWVKGHDG DPGNEMADFL AKKGASRR
Length:348
Mass (Da):39,431
Last modified:February 1, 1996 - v2
Checksum:i02403B2329126B90
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49939 Genomic DNA Translation: CAA90205.1
X57160 Genomic DNA Translation: CAA40448.1
BK006946 Genomic DNA Translation: DAA10134.1
PIRiS57601
RefSeqiNP_013961.1, NM_001182741.1

Genome annotation databases

EnsemblFungiiYMR234W; YMR234W; YMR234W
GeneIDi855274
KEGGisce:YMR234W

Similar proteinsi

Entry informationi

Entry nameiRNH1_YEAST
AccessioniPrimary (citable) accession number: Q04740
Secondary accession number(s): D6W060
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1996
Last modified: May 23, 2018
This is version 160 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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