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Q04736

- YES_MOUSE

UniProt

Q04736 - YES_MOUSE

Protein

Tyrosine-protein kinase Yes

Gene

Yes1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 159 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGRF, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis By similarity.By similarity

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei303 – 3031ATPPROSITE-ProRule annotation
    Active sitei394 – 3941Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi281 – 2899ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
    3. protein binding Source: MGI

    GO - Biological processi

    1. glucose transport Source: MGI
    2. protein autophosphorylation Source: Ensembl

    Keywords - Molecular functioni

    Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.10.2. 3474.
    ReactomeiREACT_188578. Signaling by SCF-KIT.
    REACT_196487. FCGR activation.
    REACT_204081. CD28 co-stimulation.
    REACT_227425. Regulation of KIT signaling.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyrosine-protein kinase Yes (EC:2.7.10.2)
    Alternative name(s):
    Proto-oncogene c-Yes
    p61-Yes
    Gene namesi
    Name:Yes1
    Synonyms:Yes
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:99147. Yes1.

    Subcellular locationi

    Cell membrane By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cytoplasmcytosol By similarity
    Note: Newly synthesized protein initially accumulates in the Golgi region and traffics to the plasma membrane through the exocytic pathway.By similarity

    GO - Cellular componenti

    1. cytosol Source: UniProtKB-SubCell
    2. Golgi apparatus Source: Ensembl
    3. microtubule organizing center Source: UniProtKB-SubCell
    4. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Cytoskeleton, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mice are viable, fertile, and display no apparent phenotypes. This lack of phenotype may be attributable to compensatory roles of the other SRC-family members.1 Publication

    Keywords - Diseasei

    Proto-oncogene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 541540Tyrosine-protein kinase YesPRO_0000088182Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycineBy similarity
    Lipidationi3 – 31S-palmitoyl cysteine; in membrane formBy similarity
    Modified residuei11 – 111PhosphoserineBy similarity
    Modified residuei26 – 261PhosphoserineBy similarity
    Modified residuei32 – 321PhosphotyrosineBy similarity
    Modified residuei109 – 1091PhosphoserineBy similarity
    Modified residuei192 – 1921PhosphotyrosineBy similarity
    Modified residuei193 – 1931PhosphoserineBy similarity
    Modified residuei220 – 2201PhosphotyrosineBy similarity
    Modified residuei221 – 2211PhosphotyrosineBy similarity
    Modified residuei334 – 3341PhosphotyrosineBy similarity
    Modified residuei343 – 3431PhosphotyrosineBy similarity
    Modified residuei424 – 4241Phosphotyrosine; by autocatalysisBy similarity
    Modified residuei444 – 4441PhosphotyrosineBy similarity
    Modified residuei535 – 5351Phosphotyrosine; alternate1 Publication
    Modified residuei535 – 5351Phosphotyrosine; by CSK; alternateBy similarity

    Post-translational modificationi

    Phosphorylation by CSK on the C-terminal tail maintains the enzyme in an inactive state. Autophosphorylation at Tyr-424 maintains enzyme activity by blocking CSK-mediated inhibition By similarity.By similarity
    Palmitoylation at Cys-3 promotes membrane localization.By similarity

    Keywords - PTMi

    Lipoprotein, Myristate, Palmitate, Phosphoprotein

    Proteomic databases

    MaxQBiQ04736.
    PaxDbiQ04736.
    PRIDEiQ04736.

    PTM databases

    PhosphoSiteiQ04736.

    Expressioni

    Gene expression databases

    ArrayExpressiQ04736.
    BgeeiQ04736.
    GenevestigatoriQ04736.

    Interactioni

    Subunit structurei

    Interacts with YAP1. Interacts with FASLG. Interacts with CTNND1; this interaction allows YES1-mediated activation of FYN and FER and subsequent phosphorylation of CTNND1 By similarity. Interacts with CSF1R.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi204615. 5 interactions.
    IntActiQ04736. 3 interactions.

    Structurei

    Secondary structure

    1
    541
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi93 – 986
    Beta strandi115 – 1206
    Beta strandi127 – 1348
    Beta strandi137 – 1404
    Turni142 – 1443
    Beta strandi145 – 1506
    Turni152 – 1543
    Helixi155 – 1595

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2YT6NMR-A71-167[»]
    ProteinModelPortaliQ04736.
    SMRiQ04736. Positions 83-541.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ04736.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini89 – 15062SH3PROSITE-ProRule annotationAdd
    BLAST
    Domaini156 – 25398SH2PROSITE-ProRule annotationAdd
    BLAST
    Domaini275 – 528254Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 SH2 domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH2 domain, SH3 domain

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233858.
    HOVERGENiHBG008761.
    InParanoidiQ04736.
    KOiK05705.
    OMAiIKYRTEN.
    OrthoDBiEOG7GTT2V.
    PhylomeDBiQ04736.
    TreeFamiTF351634.

    Family and domain databases

    Gene3Di3.30.505.10. 1 hit.
    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR000980. SH2.
    IPR001452. SH3_domain.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view]
    PfamiPF07714. Pkinase_Tyr. 1 hit.
    PF00017. SH2. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view]
    PRINTSiPR00401. SH2DOMAIN.
    PR00452. SH3DOMAIN.
    PR00109. TYRKINASE.
    SMARTiSM00252. SH2. 1 hit.
    SM00326. SH3. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    SSF55550. SSF55550. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS50001. SH2. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q04736-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGCIKSKENK SPAIKYTPEN LTEPVSPSAS HYGVEHATVA PTSSTKGASV    50
    NFNSLSMTPF GGSSGVTPFG GASSSFSVVS SSYPTGLTGG VTIFVALYDY 100
    EARTTEDLSF KKGERFQIIN NTEGDWWEAR SIATGKSGYI PSNYVVPADS 150
    IQAEEWYFGK MGRKDAERLL LNPGNQRGIF LVRESETTKG AYSLSIRDWD 200
    EVRGDNVKHY KIRKLDNGGY YITTRAQFDT LQKLVKHYTE HADGLCHKLT 250
    TVCPTVKPQT QGLAKDAWEI PRESLRLEVK LGQGCFGEVW MGTWNGTTKV 300
    AIKTLKPGTM MPEAFLQEAQ IMKKLRHDKL VPLYAVVSEE PIYIVTEFMS 350
    KGSLLDFLKE GDGKYLKLPQ LVDMAAQIAD GMAYIERMNY IHRDLRAANI 400
    LVGENLICKI ADFGLARLIE DNEYTARQGA KFPIKWTAPE AALYGRFTIK 450
    SDVWSFGILQ TELVTKGRVP YPGMVNREVL EQVERGYRMP CPQGCPESLH 500
    ELMNLCWKKD PDERPTFEYI QSFLEDYFTA TEPQYQPGEN L 541
    Length:541
    Mass (Da):60,630
    Last modified:January 23, 2007 - v3
    Checksum:i9A773C39D2119EA6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X67677 mRNA. Translation: CAA47909.1.
    BC010594 mRNA. Translation: AAH10594.1.
    L25762 mRNA. Translation: AAA40020.1.
    CCDSiCCDS39061.1.
    PIRiI48318. S31645.
    RefSeqiNP_001192061.1. NM_001205132.1.
    NP_001192062.1. NM_001205133.1.
    NP_033561.1. NM_009535.3.
    UniGeneiMm.4558.

    Genome annotation databases

    EnsembliENSMUST00000072311; ENSMUSP00000072154; ENSMUSG00000014932.
    ENSMUST00000168707; ENSMUSP00000132161; ENSMUSG00000014932.
    GeneIDi22612.
    KEGGimmu:22612.
    UCSCiuc008wzs.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X67677 mRNA. Translation: CAA47909.1 .
    BC010594 mRNA. Translation: AAH10594.1 .
    L25762 mRNA. Translation: AAA40020.1 .
    CCDSi CCDS39061.1.
    PIRi I48318. S31645.
    RefSeqi NP_001192061.1. NM_001205132.1.
    NP_001192062.1. NM_001205133.1.
    NP_033561.1. NM_009535.3.
    UniGenei Mm.4558.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2YT6 NMR - A 71-167 [» ]
    ProteinModelPortali Q04736.
    SMRi Q04736. Positions 83-541.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204615. 5 interactions.
    IntActi Q04736. 3 interactions.

    PTM databases

    PhosphoSitei Q04736.

    Proteomic databases

    MaxQBi Q04736.
    PaxDbi Q04736.
    PRIDEi Q04736.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000072311 ; ENSMUSP00000072154 ; ENSMUSG00000014932 .
    ENSMUST00000168707 ; ENSMUSP00000132161 ; ENSMUSG00000014932 .
    GeneIDi 22612.
    KEGGi mmu:22612.
    UCSCi uc008wzs.2. mouse.

    Organism-specific databases

    CTDi 7525.
    MGIi MGI:99147. Yes1.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233858.
    HOVERGENi HBG008761.
    InParanoidi Q04736.
    KOi K05705.
    OMAi IKYRTEN.
    OrthoDBi EOG7GTT2V.
    PhylomeDBi Q04736.
    TreeFami TF351634.

    Enzyme and pathway databases

    BRENDAi 2.7.10.2. 3474.
    Reactomei REACT_188578. Signaling by SCF-KIT.
    REACT_196487. FCGR activation.
    REACT_204081. CD28 co-stimulation.
    REACT_227425. Regulation of KIT signaling.

    Miscellaneous databases

    EvolutionaryTracei Q04736.
    NextBioi 302969.
    PROi Q04736.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q04736.
    Bgeei Q04736.
    Genevestigatori Q04736.

    Family and domain databases

    Gene3Di 3.30.505.10. 1 hit.
    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR000980. SH2.
    IPR001452. SH3_domain.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view ]
    Pfami PF07714. Pkinase_Tyr. 1 hit.
    PF00017. SH2. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00401. SH2DOMAIN.
    PR00452. SH3DOMAIN.
    PR00109. TYRKINASE.
    SMARTi SM00252. SH2. 1 hit.
    SM00326. SH3. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    SSF55550. SSF55550. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS50001. SH2. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and analysis of cDNA encoding the murine c-yes tyrosine protein kinase."
      Klages S., Adam D., Eiseman E., Fargnoli J., Dymecki S.M., Desiderio S.V., Bolen J.B.
      Oncogene 8:713-719(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Czech II.
      Tissue: Mammary gland.
    3. "Protein tyrosine kinases transcribed in a murine thymic medullary epithelial cell line."
      Hebert B., Bergeron J., Tijssen P., Potworowski E.F.
      Gene 143:257-260(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 391-458.
    4. "Activation of Src family kinases by colony stimulating factor-1, and their association with its receptor."
      Courtneidge S.A., Dhand R., Pilat D., Twamley G.M., Waterfield M.D., Roussel M.F.
      EMBO J. 12:943-950(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CSF1R.
    5. "Combined deficiencies of Src, Fyn, and Yes tyrosine kinases in mutant mice."
      Stein P.L., Vogel H., Soriano P.
      Genes Dev. 8:1999-2007(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    6. "Identification of autophosphorylation sites in c-Yes purified from rat liver plasma membranes."
      Ariki M., Tanabe O., Usui H., Hayashi H., Inoue R., Nishito Y., Kagamiyama H., Takeda M.
      J. Biochem. 121:104-111(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT TYR-32.
    7. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
      Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
      J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-535, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    8. "Solution structure of the SH3_1 domain of Yamaguchi sarcoma viral (v-Yes) oncogene homolog 1."
      RIKEN structural genomics initiative (RSGI)
      Submitted (APR-2008) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 62-167.

    Entry informationi

    Entry nameiYES_MOUSE
    AccessioniPrimary (citable) accession number: Q04736
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 159 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3