Q04734 (PALI_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
pH-response regulator protein palI/RIM9

Alternative name(s):
Regulator of IME2 protein 9

Gene names

Name:	RIM9
Ordered Locus Names:	YMR063W
ORF Names:	YM9916.02

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the proteolytic cleavage of the transcriptional repressor RIM101 in response to alkaline ambient pH, which is necessary for sporulation and invasive growth. Ref.1
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the palI/RIM9 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ascospore formation Inferred from mutant phenotype PubMed 8417990. Source: SGD
Cellular_component	integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD plasma membrane Inferred from direct assay PubMed 23019326. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 239	239	pH-response regulator protein palI/RIM9		PRO_0000058216
Regions
	Topological domain	1 – 3	3	Cytoplasmic Potential
	Transmembrane	4 – 24	21	Helical; Potential
	Topological domain	25 – 90	66	Extracellular Potential
	Transmembrane	91 – 111	21	Helical; Potential
	Topological domain	112 – 128	17	Cytoplasmic Potential
	Transmembrane	129 – 149	21	Helical; Potential
	Topological domain	150 – 155	6	Extracellular Potential
	Transmembrane	156 – 176	21	Helical; Potential
	Topological domain	177 – 239	63	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	Q04734 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 8310BF42D5A27E10	FASTA	239	26,697
	10 20 30 40 50 60 MVSMIHIVVF LLAITTMFEI LPLITVPVTK YLSLSSFRNH YYGLFGWCVR GQNQELMCTK 70 80 90 100 110 120 MKIGYDSTDV DSSGHVLTLP SNSKVVVSNL LVVHPISLAF TGTLLILAVI IMVTPLGDSP 130 140 150 160 170 180 EMLLFTALFS LPTFMLCLLC FLVDILLFIS KLDWPGWLML AATISVALCC SMLWVMRRVV 190 200 210 220 230 SVKKYESQQS IAHACSMEQY SISDIYQSKQ NGNSSEYEVA PTHTDSLIAP EVTYRGFIE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Proteolytic activation of Rim1p, a positive regulator of yeast sporulation and invasive growth." Li W., Mitchell A.P. Genetics 145:63-73(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Characterization of a second nuclear gene, AEP1, required for expression of the mitochondrial OLI1 gene in Saccharomyces cerevisiae." Payne M.J., Lukins H.B. Curr. Genet. 24:126-135(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 70-239.
[5]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U86641 Genomic DNA. Translation: AAC49630.1. Z48952 Genomic DNA. Translation: CAA88788.1. M80615 Unassigned DNA. No translation available. BK006946 Genomic DNA. Translation: DAA09961.1.
PIR	S52823.
RefSeq	NP_013779.1. NM_001182561.1.
3D structure databases
ProteinModelPortal	Q04734.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4447N.
IntAct	Q04734. 1 interaction.
MINT	MINT-496242.
STRING	4932.YMR063W.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR063W; YMR063W; YMR063W.
GeneID	855085.
KEGG	sce:YMR063W.
Organism-specific databases
CYGD	YMR063w.
SGD	S000004667. RIM9.
Phylogenomic databases
eggNOG	NOG12793.
GeneTree	ENSGT00530000068543.
HOGENOM	HOG000115433.
OMA	DWPGWLM.
OrthoDB	EOG4DBXQ8.
Gene expression databases
Genevestigator	Q04734.
GermOnline	YMR063W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR009571. SUR7/PalI. [Graphical view]
Pfam	PF06687. SUR7. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978378.

Entry information

Entry name

PALI_YEAST

Accession

Primary (citable) accession number: Q04734
Secondary accession number(s): D6VZN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program