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Protein

Arginine biosynthesis bifunctional protein ArgJ, mitochondrial

Gene

ARG7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of acetylglutamate from glutamate and acetyl-CoA, and of ornithine by transacetylation between acetylornithine and glutamate.UniRule annotation1 Publication

Catalytic activityi

N2-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate.UniRule annotation
Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate.UniRule annotation

Enzyme regulationi

Inhibited by ornithine.1 Publication

Kineticsi

  1. KM=8.4 mM for glutamate1 Publication
  2. KM=2.8 mM for N-acetylornithine1 Publication

    Pathwayi: L-arginine biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic).UniRule annotation
    Proteins known to be involved in this subpathway in this organism are:
    1. Arginine biosynthesis bifunctional protein ArgJ, mitochondrial (ARG7)
    This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic), the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

    Pathwayi: L-arginine biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate.UniRule annotation
    Proteins known to be involved in the 4 steps of the subpathway in this organism are:
    1. Amino-acid acetyltransferase, mitochondrial (ARG2), Arginine biosynthesis bifunctional protein ArgJ, mitochondrial (ARG7)
    2. Protein ARG5,6, mitochondrial (ARG5,6)
    3. Protein ARG5,6, mitochondrial (ARG5,6)
    4. Acetylornithine aminotransferase, mitochondrial (ARG8)
    This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sitei136Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion holeUniRule annotation1
    Sitei137Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion holeUniRule annotation1
    Binding sitei177SubstrateUniRule annotation1
    Binding sitei204SubstrateUniRule annotation1
    Active sitei215NucleophileUniRule annotation1
    Binding sitei215SubstrateUniRule annotation1
    Binding sitei301SubstrateUniRule annotation1
    Binding sitei436SubstrateUniRule annotation1
    Binding sitei441SubstrateUniRule annotation1

    GO - Molecular functioni

    • acetyl-CoA:L-glutamate N-acetyltransferase activity Source: SGD
    • glutamate N-acetyltransferase activity Source: SGD
    • methione N-acyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    • arginine biosynthetic process Source: SGD
    • ornithine biosynthetic process Source: SGD

    Keywordsi

    Molecular functionAcyltransferase, Multifunctional enzyme, Transferase
    Biological processAmino-acid biosynthesis, Arginine biosynthesis

    Enzyme and pathway databases

    BioCyciYEAST:YMR062C-MONOMER
    UniPathwayiUPA00068; UER00106
    UPA00068; UER00111

    Protein family/group databases

    MEROPSiT05.001

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Arginine biosynthesis bifunctional protein ArgJ, mitochondrialUniRule annotation
    Alternative name(s):
    Extracellular mutant protein 40
    Cleaved into the following 2 chains:
    Arginine biosynthesis bifunctional protein ArgJ alpha chainUniRule annotation
    Arginine biosynthesis bifunctional protein ArgJ beta chainUniRule annotation
    Including the following 2 domains:
    Glutamate N-acetyltransferaseUniRule annotation (EC:2.3.1.35UniRule annotation)
    Short name:
    GATUniRule annotation
    Alternative name(s):
    Ornithine acetyltransferaseUniRule annotation
    Short name:
    OATaseUniRule annotation
    Ornithine transacetylaseUniRule annotation
    Amino-acid acetyltransferaseUniRule annotation (EC:2.3.1.1UniRule annotation)
    Alternative name(s):
    N-acetylglutamate synthaseUniRule annotation
    Short name:
    AGSUniRule annotation
    Gene namesi
    Name:ARG7UniRule annotation
    Synonyms:ECM40
    Ordered Locus Names:YMR062C
    ORF Names:YM9916.01C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XIII

    Organism-specific databases

    EuPathDBiFungiDB:YMR062C
    SGDiS000004666 ARG7

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi215T → A: Blocks autocatalytic processing of the precursor protein. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transit peptidei1 – 8MitochondrionUniRule annotation1 Publication8
    ChainiPRO_00000022839 – 214Arginine biosynthesis bifunctional protein ArgJ alpha chainUniRule annotationAdd BLAST206
    ChainiPRO_0000002284215 – 441Arginine biosynthesis bifunctional protein ArgJ beta chainUniRule annotationAdd BLAST227

    Post-translational modificationi

    The alpha and beta chains are autoproteolytically processed from a single precursor protein within the mitochondrion.UniRule annotation1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sitei214 – 215Cleavage; by autolysis2

    Keywords - PTMi

    Autocatalytic cleavage

    Proteomic databases

    MaxQBiQ04728
    PaxDbiQ04728
    PRIDEiQ04728

    Interactioni

    Subunit structurei

    Heterodimer of an alpha and a beta chain.

    Protein-protein interaction databases

    BioGridi35237, 54 interactors
    IntActiQ04728, 1 interactor
    STRINGi4932.YMR062C

    Structurei

    3D structure databases

    ProteinModelPortaliQ04728
    SMRiQ04728
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ArgJ family.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    HOGENOMiHOG000022798
    InParanoidiQ04728
    KOiK00620
    OMAiGMIAPNM
    OrthoDBiEOG092C2OPF

    Family and domain databases

    CDDicd02152 OAT, 1 hit
    Gene3Di3.60.70.12, 1 hit
    HAMAPiMF_01106 ArgJ, 1 hit
    InterProiView protein in InterPro
    IPR002813 Arg_biosynth_ArgJ
    IPR016117 ArgJ-like_dom_sf
    PANTHERiPTHR23100 PTHR23100, 1 hit
    PfamiView protein in Pfam
    PF01960 ArgJ, 1 hit
    SUPFAMiSSF56266 SSF56266, 1 hit
    TIGRFAMsiTIGR00120 ArgJ, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q04728-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRISSTLLQR SKQLIDKYAL YVPKTGSFPK GFEVGYTASG VKKNGSLDLG
    60 70 80 90 100
    VILNTNKSRP STAAAVFTTN KFKAAPVLTS KKVLETARGK NINAIVVNSG
    110 120 130 140 150
    CANSVTGDLG MKDAQVMIDL VNDKIGQKNS TLVMSTGVIG QRLQMDKIST
    160 170 180 190 200
    GINKIFGEEK FGSDFNSWLN VAKSICTTDT FPKLVTSRFK LPSGTEYTLT
    210 220 230 240 250
    GMAKGAGMIC PNMATLLGFI VTDLPIESKA LQKMLTFATT RSFNCISVDG
    260 270 280 290 300
    DMSTNDTICM LANGAIDTKE INEDSKDFEQ VKLQVTEFAQ RLAQLVVRDG
    310 320 330 340 350
    EGSTKFVTVN VKNALHFEDA KIIAESISNS MLVKTALYGQ DANWGRILCA
    360 370 380 390 400
    IGYAKLNDLK SLDVNKINVS FIATDNSEPR ELKLVANGVP QLEIDETRAS
    410 420 430 440
    EILALNDLEV SVDLGTGDQA AQFWTCDLSH EYVTINGDYR S
    Length:441
    Mass (Da):47,849
    Last modified:November 1, 1997 - v1
    Checksum:i7AC03B7A72E3CE46
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U90438 Genomic DNA Translation: AAB49897.1
    Z48952 Genomic DNA Translation: CAA88787.1
    Z49703 Genomic DNA Translation: CAA89772.1
    BK006946 Genomic DNA Translation: DAA09960.1
    PIRiS52822
    RefSeqiNP_013778.1, NM_001182560.1

    Genome annotation databases

    EnsemblFungiiYMR062C; YMR062C; YMR062C
    GeneIDi855084
    KEGGisce:YMR062C

    Similar proteinsi

    Entry informationi

    Entry nameiARGJ_YEAST
    AccessioniPrimary (citable) accession number: Q04728
    Secondary accession number(s): D6VZN6, Q04682, Q09168
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: March 28, 2018
    This is version 152 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

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