Reviewed, UniProtKB/Swiss-Prot Q04723 (PEPC_LACLC)

Names and origin

Protein names

Recommended name:
    Aminopeptidase C
    EC=3.4.22.40
Alternative name(s):
    Bleomycin hydrolase

Gene names

Name:

pepC

Organism

Lactococcus lactis subsp. cremoris (Streptococcus cremoris)

Taxonomic identifier

1359 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hydrolyzes naphthylamide-substituted amino acids as well as di- and tripeptides in which the half-cystine residue is involved in a disulfide loop, notably in oxytocin and vasopressin. Has also a bleomycin hydrolase activity.
Catalytic activity	Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred.
Subunit structure	Homohexamer.
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Molecular function	Aminopeptidase Hydrolase Protease Thiol protease
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 436	435	Aminopeptidase C		PRO_0000050593
Sites
	Active site	68	1	By similarity
	Active site	356	1	By similarity
	Active site	378	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q04723-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 1464F6A6E873FCA1	FASTA	436	49,827
	10 20 30 40 50 60 MTVTSDFTQK LYENFAENTK LRAVENAVTK NGLLSSLEVR GSHAANLPEF SLDLTKDPVT 70 80 90 100 110 120 NQKQSGRCWM FAALNTFRHK FINEFKTEDF EFSQAYTFFW DKYEKSNWFM EQIIGDVAMD 130 140 150 160 170 180 DRRLKFLLQT PQQDGGQWDM MVAIFDKYGI VPKAVYPESQ ASSSSRELNQ YLNKLLRQDA 190 200 210 220 230 240 EILRYTIEQD GDVQAVKEEL LQEVFNFLAV TLGLPPQNFE FAFRNKDNEY KKFVGTPKEF 250 260 270 280 290 300 YNEYVGIDLN NYVSVINAPT ADKPYNKSYT VEFLGNVVGG KEVKHLNVEM DRFKKLAIAQ 310 320 330 340 350 360 MQAGETVWFG CDVGQESNRS AGLLTMDSYD FKSSLDIEFT QSKAGRLDYG ESLMTHAMVL 370 380 390 400 410 420 AGVDLDADGN STKWKVENSW GKDAGQKGYF VASDEWMDEY TYQIVVRKDL LSEEELAAYE 430 AKPQVLLPWD PMGALA

References

[1]	"Cloning and sequencing of pepC, a cysteine aminopeptidase gene from Lactococcus lactis subsp. cremoris AM2." Chapot-Chartier M.P., Nardi M., Chopin M.-C., Chopin A., Gripon J.-C. Appl. Environ. Microbiol. 59:330-333(1993) [PubMed: 8439160] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-16. Strain: AM2.
[2]	"Catalytic properties of the cysteine aminopeptidase PepC, a bacterial bleomycin hydrolase." Mistou M.Y., Gripon J.-C. Biochim. Biophys. Acta 1383:63-70(1998) [PubMed: 9546047] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M86245 Genomic DNA. Translation: AAA74514.1.
PIR	B48957.
3D structure databases
HSSP	HSSP built from PDB template 1CB5 based on UniProtKB Q13867.
ModBase	Search...
Protein family/group databases
MEROPS	C01.086.
Enzyme and pathway databases
BRENDA	3.4.22.40. 289716.
Family and domain databases
InterPro	IPR000169. Pept_cys_AS. IPR004134. Peptidase_C1B. [Graphical view]
PANTHER	PTHR10363. Peptidase_C1B. 1 hit.
Pfam	PF03051. Peptidase_C1_2. 1 hit. [Graphical view]
PIRSF	PIRSF005700. PepC. 1 hit.
PROSITE	PS00640. THIOL_PROTEASE_ASN. False negative. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PEPC_LACLC

Accession

Primary (citable) accession number: Q04723

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	January 23, 2007
Last modified:	January 20, 2009
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)