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Q04718 (FUMA_GEOSE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fumarate hydratase class I, aerobic

Short name=Fumarase
EC=4.2.1.2
Gene names
Name:fumA
OrganismGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifier1422 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Protein attributes

Sequence length514 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

(S)-malate = fumarate + H2O.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.

Subunit structure

Homodimer.

Sequence similarities

Belongs to the class-I fumarase family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
   Molecular functionLyase
Gene Ontology (GO)
   Biological_processtricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-KW

fumarate hydratase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 514514Fumarate hydratase class I, aerobic
PRO_0000195655

Sites

Active site3581 Potential
Metal binding621Iron-sulfur Potential
Metal binding1871Iron-sulfur Potential
Metal binding2741Iron-sulfur Potential
Binding site4221Substrate Potential

Sequences

Sequence LengthMass (Da)Tools
Q04718 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 238F2890E8CC54E1

FASTA51456,792
        10         20         30         40         50         60 
MEKLYESMYQ LIVETSTKLP KDVRRAILKA KQRENAGTRA AMSLATITEN IKMADENVSP 

        70         80         90        100        110        120 
ICQDTGLPTF KIKVPVGINQ IQIKETIKKA IAQATKDGKL RPNSVDSLTG ENSGDNLGEG 

       130        140        150        160        170        180 
LPVVKFEQWE KDYMDVRLIL KGGGCENKNI QYSLPCELEG LGRAGRDLDG IRKCILHAVY 

       190        200        210        220        230        240 
QAQGQGCSAG FIGVGIGGDR SAGYDLAKEQ LFREVDDVNP NEELRQLEEY IMENANKLGI 

       250        260        270        280        290        300 
GTMGFGGETT LLGCKVGAMH RIPASFFVSV AYNCWAFRRL GVHIDPNTGE IIKWLYQDGE 

       310        320        330        340        350        360 
DVDFQENAAQ EEHLASTDSE RRVITLQAPI TEEQIRELKV GDVVRINGII YTGRDAIHKY 

       370        380        390        400        410        420 
LMDHDAPVDL NGQIIYHCGP VMLKDENGNW EVKAAGPTTS IREEPYQGDI MKKFGIRAVM 

       430        440        450        460        470        480 
GKGGMGQKTL QALKEHGGVY LNAIGGAAQY YADCIEAVEG VDLLEFGIPE AMWHLRVKDF 

       490        500        510 
TAVVTMDSHG NSLHEDIEKS SLEKLSQFKE PVFS 

« Hide

References

[1]"Molecular and enzymological evidence for two classes of fumarase in Bacillus stearothermophilus (var. non-diastaticus)."
Reaney S.K., Bungard S.J., Guest J.R.
J. Gen. Microbiol. 139:403-416(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DSM 2334 / Var. Non-diastaticus.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L05611 Genomic DNA. Translation: AAA72317.1.
PIRA47692.

3D structure databases

ProteinModelPortalQ04718.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00223; UER01007.

Family and domain databases

Gene3D3.20.130.10. 1 hit.
InterProIPR004646. Fe-S_hydro-lyase_TtdA-typ_cat.
IPR004647. Fe-S_hydro-lyase_TtdB-typ_cat.
IPR011167. Fe_dep_fumarate_hydratase.
[Graphical view]
PfamPF05681. Fumerase. 1 hit.
PF05683. Fumerase_C. 1 hit.
[Graphical view]
PIRSFPIRSF001394. Fe_dep_fumar_hy. 1 hit.
SUPFAMSSF117457. SSF117457. 1 hit.
TIGRFAMsTIGR00722. ttdA_fumA_fumB. 1 hit.
TIGR00723. ttdB_fumA_fumB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameFUMA_GEOSE
AccessionPrimary (citable) accession number: Q04718
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: February 19, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways