ID   RRN11_YEAST             Reviewed;         507 AA.
AC   Q04712; D6VZD2;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   27-MAR-2024, entry version 160.
DE   RecName: Full=RNA polymerase I-specific transcription initiation factor RRN11;
GN   Name=RRN11; OrderedLocusNames=YML043C; ORFNames=YM9827.09C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169872;
RA   Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA   Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA   Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA   Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL   Nature 387:90-93(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [4]
RP   IDENTIFICATION IN THE CF COMPLEX.
RX   PubMed=7958901; DOI=10.1101/gad.8.19.2349;
RA   Keys D.A., Vu L., Steffan J.S., Dodd J.A., Yamamoto R.T., Nogi Y.,
RA   Nomura M.;
RT   "RRN6 and RRN7 encode subunits of a multiprotein complex essential for the
RT   initiation of rDNA transcription by RNA polymerase I in Saccharomyces
RT   cerevisiae.";
RL   Genes Dev. 8:2349-2362(1994).
RN   [5]
RP   IDENTIFICATION IN THE CF COMPLEX, AND INTERACTION WITH RRN6; RRN7 AND
RP   SPT15.
RX   PubMed=8702872; DOI=10.1074/jbc.271.35.21062;
RA   Lalo D., Steffan J.S., Dodd J.A., Nomura M.;
RT   "RRN11 encodes the third subunit of the complex containing Rrn6p and Rrn7p
RT   that is essential for the initiation of rDNA transcription by yeast RNA
RT   polymerase I.";
RL   J. Biol. Chem. 271:21062-21067(1996).
RN   [6]
RP   FUNCTION OF THE CF COMPLEX.
RX   PubMed=8887672; DOI=10.1128/mcb.16.11.6436;
RA   Lin C.W., Moorefield B., Payne J., Aprikian P., Mitomo K., Reeder R.H.;
RT   "A novel 66-kilodalton protein complexes with Rrn6, Rrn7, and TATA-binding
RT   protein to promote polymerase I transcription initiation in Saccharomyces
RT   cerevisiae.";
RL   Mol. Cell. Biol. 16:6436-6443(1996).
RN   [7]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [8]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
CC   -!- FUNCTION: Acts as a component of the core factor (CF) complex which is
CC       essential for the initiation of rDNA transcription by RNA polymerase I.
CC       After binding of UAF (upstream activation factor) to an upstream
CC       element of the promoter, CF is recruited in a SPT15/TBP-dependent
CC       manner to form a preinitiation complex. {ECO:0000269|PubMed:8887672}.
CC   -!- SUBUNIT: Component of the core factor (CF) complex, which consists of
CC       RRN6, RRN7 and RRN11. The CF heterotrimer may further dimerize to form
CC       a hexamer. RRN11 interacts with RRN6, RRN7 and SPT15.
CC       {ECO:0000269|PubMed:7958901, ECO:0000269|PubMed:8702872}.
CC   -!- INTERACTION:
CC       Q04712; P32786: RRN6; NbExp=2; IntAct=EBI-27790, EBI-15986;
CC       Q04712; P40992: RRN7; NbExp=2; IntAct=EBI-27790, EBI-15990;
CC       Q04712; P13393: SPT15; NbExp=2; IntAct=EBI-27790, EBI-19129;
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:14562095}.
CC   -!- MISCELLANEOUS: Present with 476 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
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DR   EMBL; Z47816; CAA87831.1; -; Genomic_DNA.
DR   EMBL; AY692808; AAT92827.1; -; Genomic_DNA.
DR   EMBL; BK006946; DAA09856.1; -; Genomic_DNA.
DR   PIR; S50949; S50949.
DR   RefSeq; NP_013669.1; NM_001182401.1.
DR   PDB; 5N5Y; EM; 7.70 A; R=1-507.
DR   PDB; 5N5Z; EM; 7.70 A; R=1-507.
DR   PDB; 5N60; EM; 7.70 A; R=1-507.
DR   PDB; 5N61; EM; 3.40 A; R=1-507.
DR   PDB; 5O7X; X-ray; 3.20 A; C/F/I/L/O/R=1-507.
DR   PDB; 5OA1; EM; 4.40 A; W=1-507.
DR   PDB; 5W5Y; EM; 3.80 A; Q=1-507.
DR   PDB; 5W64; EM; 4.20 A; Q=1-507.
DR   PDB; 5W65; EM; 4.30 A; Q=1-507.
DR   PDB; 5W66; EM; 3.90 A; Q=1-507.
DR   PDB; 6RQH; EM; 3.70 A; R=1-507.
DR   PDB; 6RQL; EM; 2.90 A; R=1-507.
DR   PDB; 6RRD; EM; 3.10 A; R=1-507.
DR   PDB; 6RUI; EM; 2.70 A; R=1-507.
DR   PDB; 6RUO; EM; 3.50 A; R=1-507.
DR   PDB; 6RWE; EM; 3.00 A; R=1-507.
DR   PDB; 6TPS; EM; 3.54 A; R=1-507.
DR   PDBsum; 5N5Y; -.
DR   PDBsum; 5N5Z; -.
DR   PDBsum; 5N60; -.
DR   PDBsum; 5N61; -.
DR   PDBsum; 5O7X; -.
DR   PDBsum; 5OA1; -.
DR   PDBsum; 5W5Y; -.
DR   PDBsum; 5W64; -.
DR   PDBsum; 5W65; -.
DR   PDBsum; 5W66; -.
DR   PDBsum; 6RQH; -.
DR   PDBsum; 6RQL; -.
DR   PDBsum; 6RRD; -.
DR   PDBsum; 6RUI; -.
DR   PDBsum; 6RUO; -.
DR   PDBsum; 6RWE; -.
DR   PDBsum; 6TPS; -.
DR   AlphaFoldDB; Q04712; -.
DR   EMDB; EMD-10006; -.
DR   EMDB; EMD-10007; -.
DR   EMDB; EMD-10038; -.
DR   EMDB; EMD-10544; -.
DR   EMDB; EMD-3590; -.
DR   EMDB; EMD-3591; -.
DR   EMDB; EMD-3592; -.
DR   EMDB; EMD-3593; -.
DR   EMDB; EMD-3727; -.
DR   EMDB; EMD-4982; -.
DR   EMDB; EMD-4984; -.
DR   EMDB; EMD-4987; -.
DR   EMDB; EMD-8771; -.
DR   EMDB; EMD-8772; -.
DR   EMDB; EMD-8774; -.
DR   EMDB; EMD-8775; -.
DR   EMDB; EMD-8776; -.
DR   EMDB; EMD-8777; -.
DR   SMR; Q04712; -.
DR   BioGRID; 35126; 80.
DR   ComplexPortal; CPX-1836; RNA polymerase I core factor complex.
DR   DIP; DIP-4542N; -.
DR   IntAct; Q04712; 9.
DR   MINT; Q04712; -.
DR   STRING; 4932.YML043C; -.
DR   iPTMnet; Q04712; -.
DR   PaxDb; 4932-YML043C; -.
DR   PeptideAtlas; Q04712; -.
DR   EnsemblFungi; YML043C_mRNA; YML043C; YML043C.
DR   GeneID; 854964; -.
DR   KEGG; sce:YML043C; -.
DR   AGR; SGD:S000004507; -.
DR   SGD; S000004507; RRN11.
DR   VEuPathDB; FungiDB:YML043C; -.
DR   eggNOG; ENOG502R1IK; Eukaryota.
DR   HOGENOM; CLU_034126_0_0_1; -.
DR   InParanoid; Q04712; -.
DR   OMA; EVWFIYA; -.
DR   OrthoDB; 1974231at2759; -.
DR   BioCyc; YEAST:G3O-32641-MONOMER; -.
DR   BioGRID-ORCS; 854964; 0 hits in 10 CRISPR screens.
DR   PRO; PR:Q04712; -.
DR   Proteomes; UP000002311; Chromosome XIII.
DR   RNAct; Q04712; Protein.
DR   GO; GO:0005730; C:nucleolus; IDA:SGD.
DR   GO; GO:0005634; C:nucleus; NAS:ComplexPortal.
DR   GO; GO:0070860; C:RNA polymerase I core factor complex; IDA:SGD.
DR   GO; GO:0001164; F:RNA polymerase I core promoter sequence-specific DNA binding; IDA:SGD.
DR   GO; GO:0001181; F:RNA polymerase I general transcription initiation factor activity; IEA:InterPro.
DR   GO; GO:0017025; F:TBP-class protein binding; IDA:SGD.
DR   GO; GO:0042790; P:nucleolar large rRNA transcription by RNA polymerase I; IDA:SGD.
DR   InterPro; IPR007224; TIF_Rrn11.
DR   InterPro; IPR016850; TIF_Rrn11_budding_yeast.
DR   PANTHER; PTHR28244; RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN11; 1.
DR   PANTHER; PTHR28244:SF1; RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN11; 1.
DR   Pfam; PF04090; Rrn11; 1.
DR   PIRSF; PIRSF027133; Rrn11; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..507
FT                   /note="RNA polymerase I-specific transcription initiation
FT                   factor RRN11"
FT                   /id="PRO_0000203257"
FT   REGION          37..76
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          89..124
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   HELIX           11..37
FT                   /evidence="ECO:0007829|PDB:6RUI"
FT   HELIX           75..83
FT                   /evidence="ECO:0007829|PDB:5O7X"
FT   HELIX           127..130
FT                   /evidence="ECO:0007829|PDB:6RUI"
FT   STRAND          135..139
FT                   /evidence="ECO:0007829|PDB:6RUI"
FT   STRAND          145..147
FT                   /evidence="ECO:0007829|PDB:6RUI"
FT   HELIX           159..165
FT                   /evidence="ECO:0007829|PDB:6RUI"
FT   HELIX           167..173
FT                   /evidence="ECO:0007829|PDB:6RUI"
FT   HELIX           176..179
FT                   /evidence="ECO:0007829|PDB:6RUI"
FT   HELIX           182..191
FT                   /evidence="ECO:0007829|PDB:6RUI"
FT   STRAND          196..199
FT                   /evidence="ECO:0007829|PDB:6RWE"
FT   TURN            201..203
FT                   /evidence="ECO:0007829|PDB:6RUI"
FT   HELIX           210..225
FT                   /evidence="ECO:0007829|PDB:6RUI"
FT   HELIX           229..239
FT                   /evidence="ECO:0007829|PDB:6RUI"
FT   HELIX           247..252
FT                   /evidence="ECO:0007829|PDB:6RUI"
FT   HELIX           255..258
FT                   /evidence="ECO:0007829|PDB:6RUI"
FT   STRAND          262..265
FT                   /evidence="ECO:0007829|PDB:5O7X"
FT   HELIX           268..277
FT                   /evidence="ECO:0007829|PDB:6RUI"
FT   STRAND          278..280
FT                   /evidence="ECO:0007829|PDB:5O7X"
FT   HELIX           295..298
FT                   /evidence="ECO:0007829|PDB:6RUI"
FT   STRAND          301..303
FT                   /evidence="ECO:0007829|PDB:5O7X"
FT   HELIX           307..321
FT                   /evidence="ECO:0007829|PDB:6RUI"
FT   HELIX           346..354
FT                   /evidence="ECO:0007829|PDB:6RUI"
FT   HELIX           363..378
FT                   /evidence="ECO:0007829|PDB:6RUI"
FT   TURN            379..383
FT                   /evidence="ECO:0007829|PDB:6RUO"
FT   HELIX           398..420
FT                   /evidence="ECO:0007829|PDB:6RUI"
FT   STRAND          421..423
FT                   /evidence="ECO:0007829|PDB:6RUI"
FT   HELIX           428..440
FT                   /evidence="ECO:0007829|PDB:6RUI"
SQ   SEQUENCE   507 AA;  59254 MW;  726B0963F0E79783 CRC64;
     MFEVPITLTN RKFAQRRKLK YQYINYISRR FDRISKKSTT TDSLPTPENS AAENNDEEEG
     QNSEAGTYRR SVLQQKKRRR ERHWRSVVGE IYSTTESETD SQEEETEEGG EHDTGIDKED
     SDEERKFWKK YEKPEKSFEI WRTVSSQNKQ PINKQKMTYH NFKKIEKIPL RKMEIPLLHC
     TKENKLYFQS ISRGLEPLKT STSEVRNYRT RHIVTLTDLL HLNVSRHNWS LAYKIFATLI
     RIPGVQIKSL WGIGVEILDN LSNSSSGLDF LQWMCQIYSS KSRFVQNINY RSIVPPFQTG
     SRTHTAKFAI TYLWSSLINC QKSMEPSSNI IDKPFDTEND LLQELIDKIS EWVLTPPFME
     DAEVWFIYAS CHLLKADTLS RQFVNDNKNN DLIGLDRDIK INQVIKHIHY VRTFLKICLD
     KGGFAVPSRL IENQLKSFES RLYGEAQDIQ ERDVANVYDS IDNSSVENSF GDVYETNAEF
     LDTQLMDLSP EDNGLDEMHY SDEDSSE
//