Q04712 (RRN11_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA polymerase I-specific transcription initiation factor RRN11

Gene names

Name:	RRN11
Ordered Locus Names:	YML043C
ORF Names:	YM9827.09C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	507 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as component of the core factor (CF) complex which is essential for the initiation of rDNA transcription by RNA polymerase I. After binding of UAF (upstream activation factor) to an upstream element of the promoter, CF is recruited in a SPT15/TBP-dependent manner to form a preinitiation complex. Ref.6
Subunit structure	Component of the core factor (CF) complex, which consists of RRN6, RRN7 and RRN11. The CF heterotrimer may further dimerize to form a hexamer. RRN11 interacts with RRN6, RRN7 and SPT15. Ref.4 Ref.5
Subcellular location	Nucleus › nucleolus Ref.7.
Miscellaneous	Present with 476 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	RNA polymerase I core factor complex Inferred from direct assay Ref.5Ref.6. Source: SGD
Molecular_function	RNA polymerase I CORE element sequence-specific DNA binding Inferred from direct assay PubMed 18451108. Source: SGD RNA polymerase I CORE element sequence-specific DNA binding transcription factor recruiting transcription factor activity Inferred by curator. Source: SGD TBP-class protein binding Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RRN6	P32786	2	EBI-27790,EBI-15986
RRN7	P40992	2	EBI-27790,EBI-15990
SPT15	P13393	2	EBI-27790,EBI-19129

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 507	507	RNA polymerase I-specific transcription initiation factor RRN11		PRO_0000203257

Sequences

	Sequence		Length	Mass (Da)
	Q04712 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 726B0963F0E79783	FASTA	507	59,254
	10 20 30 40 50 60 MFEVPITLTN RKFAQRRKLK YQYINYISRR FDRISKKSTT TDSLPTPENS AAENNDEEEG 70 80 90 100 110 120 QNSEAGTYRR SVLQQKKRRR ERHWRSVVGE IYSTTESETD SQEEETEEGG EHDTGIDKED 130 140 150 160 170 180 SDEERKFWKK YEKPEKSFEI WRTVSSQNKQ PINKQKMTYH NFKKIEKIPL RKMEIPLLHC 190 200 210 220 230 240 TKENKLYFQS ISRGLEPLKT STSEVRNYRT RHIVTLTDLL HLNVSRHNWS LAYKIFATLI 250 260 270 280 290 300 RIPGVQIKSL WGIGVEILDN LSNSSSGLDF LQWMCQIYSS KSRFVQNINY RSIVPPFQTG 310 320 330 340 350 360 SRTHTAKFAI TYLWSSLINC QKSMEPSSNI IDKPFDTEND LLQELIDKIS EWVLTPPFME 370 380 390 400 410 420 DAEVWFIYAS CHLLKADTLS RQFVNDNKNN DLIGLDRDIK INQVIKHIHY VRTFLKICLD 430 440 450 460 470 480 KGGFAVPSRL IENQLKSFES RLYGEAQDIQ ERDVANVYDS IDNSSVENSF GDVYETNAEF 490 500 LDTQLMDLSP EDNGLDEMHY SDEDSSE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"RRN6 and RRN7 encode subunits of a multiprotein complex essential for the initiation of rDNA transcription by RNA polymerase I in Saccharomyces cerevisiae." Keys D.A., Vu L., Steffan J.S., Dodd J.A., Yamamoto R.T., Nogi Y., Nomura M. Genes Dev. 8:2349-2362(1994) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE CF COMPLEX.
[5]	"RRN11 encodes the third subunit of the complex containing Rrn6p and Rrn7p that is essential for the initiation of rDNA transcription by yeast RNA polymerase I." Lalo D., Steffan J.S., Dodd J.A., Nomura M. J. Biol. Chem. 271:21062-21067(1996) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE CF COMPLEX, INTERACTION WITH RRN6; RRN7 AND SPT15.
[6]	"A novel 66-kilodalton protein complexes with Rrn6, Rrn7, and TATA-binding protein to promote polymerase I transcription initiation in Saccharomyces cerevisiae." Lin C.W., Moorefield B., Payne J., Aprikian P., Mitomo K., Reeder R.H. Mol. Cell. Biol. 16:6436-6443(1996) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE CF COMPLEX.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z47816 Genomic DNA. Translation: CAA87831.1. AY692808 Genomic DNA. Translation: AAT92827.1. BK006946 Genomic DNA. Translation: DAA09856.1.
PIR	S50949.
RefSeq	NP_013669.1. NM_001182401.1.
3D structure databases
ProteinModelPortal	Q04712.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4542N.
IntAct	Q04712. 8 interactions.
MINT	MINT-526952.
STRING	4932.YML043C.
Proteomic databases
PaxDb	Q04712.
PeptideAtlas	Q04712.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML043C; YML043C; YML043C.
GeneID	854964.
KEGG	sce:YML043C.
Organism-specific databases
CYGD	YML043c.
SGD	S000004507. RRN11.
Phylogenomic databases
eggNOG	NOG75014.
HOGENOM	HOG000141986.
KO	K15222.
OMA	MFEVPIT.
OrthoDB	EOG43R6X4.
Gene expression databases
Genevestigator	Q04712.
GermOnline	YML043C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007224. TIF_Rrn11. IPR016850. TIF_Rrn11_budding_yeast. [Graphical view]
Pfam	PF04090. RNA_pol_I_TF. 1 hit. [Graphical view]
PIRSF	PIRSF027133. Rrn11. 1 hit.
ProtoNet	Search...
Other
NextBio	978053.

Entry information

Entry name

RRN11_YEAST

Accession

Primary (citable) accession number: Q04712
Secondary accession number(s): D6VZD2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program