Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q046F8 (GLYA_LACGA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyridoxal-phosphate-dependent serine hydroxymethyltransferase
Short name=SHMT
Short name=Serine methylase
EC=2.1.2.1
Gene names
Name:glyA
Ordered Locus Names:LGAS_0256
OrganismLactobacillus gasseri (strain ATCC 33323 / DSM 20243) [Complete proteome] [HAMAP]
Taxonomic identifier324831 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length411 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate serving as the one-carbon carrier By similarity.

Catalytic activity

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051

Cofactor

Pyridoxal phosphate By similarity. HAMAP MF_00051

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051

Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity HAMAP MF_00051.

Sequence similarities

Belongs to the SHMT family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 411411Pyridoxal-phosphate-dependent serine hydroxymethyltransferase
PRO_1000006270

Regions

Region120 – 1223Substrate binding By similarity

Sites

Binding site301Pyridoxal phosphate By similarity
Binding site501Pyridoxal phosphate By similarity
Binding site521Substrate By similarity
Binding site591Substrate binding By similarity
Binding site601Pyridoxal phosphate By similarity
Binding site941Pyridoxal phosphate By similarity
Binding site1711Pyridoxal phosphate By similarity
Binding site1991Pyridoxal phosphate By similarity
Binding site2241Pyridoxal phosphate By similarity
Binding site2311Pyridoxal phosphate By similarity
Binding site2571Pyridoxal phosphate; via amide nitrogen and carbonyl oxygen By similarity
Binding site3581Pyridoxal phosphate By similarity

Amino acid modifications

Modified residue2251N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q046F8 [UniParc].

Last modified November 14, 2006. Version 1.
Checksum: DB790C13AE3467AF

FASTA41144,915
        10         20         30         40         50         60 
MNYGEKAPAL WDAIKNEEKR QEDTIELIAS ENIVSDAVRE AQGSVLTNKY AEGYPGKRYY 

        70         80         90        100        110        120 
GGCQYIDQVE QLAIDYAKKL FNAEYANVQP HSGSQANMTV YNALLKPGDT ILGMGMDAGG 

       130        140        150        160        170        180 
HLTHGSKVNF SGKIFNSVSY DLNPETEELD FEKIRQIALE NKPKLIIAGA SAYSRIIDWQ 

       190        200        210        220        230        240 
KFREIADEVG AYLMVDMAHI AGLVATGAHP SPIPVADVVT TTTHKTLRGP RGGMILSNNK 

       250        260        270        280        290        300 
KLGKKIDSAL FPGTQGGPLE HVIAAKAQAF YEDLQPEFST YIEQVVKNAQ AMADEFKKSE 

       310        320        330        340        350        360 
NIRVVSGGTD NHLMIVDITK TGVTGKDAQN LLDSVNITTN KESIPGDTRS PFITSGLRIG 

       370        380        390        400        410 
TPAITSRGFK ENDAREVARI IIKVLDNPED KDVLTEAKSS VKSLVDKHQI K

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000413 Genomic DNA. Translation: ABJ59664.1.
RefSeqYP_814102.1. NC_008530.1.

3D structure databases

ProteinModelPortalQ046F8.
SMRQ046F8. Positions 9-385.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ046F8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4438799.
GenomeReviewsGene locus LGAS_0256 in contig CP000413_GR.
KEGGlga:LGAS_0256.
NMPDRfig|1596.1.peg.1233.
PATRIC22228656. VBILacGas62225_0252.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0112.
HOGENOMHBG301263.
OMAGTSNHLM.
PhylomeDBQ046F8.
ProtClustDBPRK00011.

Enzyme and pathway databases

BioCycLGAS324831:LGAS_0256-MONOMER.

Family and domain databases

HAMAPMF_00051. SHMT.
[Tree]
InterProIPR015424. PyrdxlP-dep_Trfase_major_dom.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
Gene3DG3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KOK00600.
PANTHERPTHR11680. Gly_HO-Metrfase. 1 hit.
PfamPF00464. SHMT. 1 hit.
[Graphical view]
PIRSFPIRSF000412. SHMT. 1 hit.
SUPFAMSSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITEPS00096. SHMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLYA_LACGA
AccessionPrimary (citable) accession number: Q046F8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 14, 2006
Last modified: January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents