Q04697 (GSF2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucose-signaling factor 2

Alternative name(s):
Extracellular mutant protein 6

Gene names

Name:	GSF2
Synonyms:	ECM6, EFF2
Ordered Locus Names:	YML048W
ORF Names:	YM9827.04

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in the secretion of hexose transporters from the endoplasmic reticulum. Involved in secretion of GAL2 and HXT1. Ref.5 Ref.6
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein Potential Ref.6 Ref.7.
Miscellaneous	Present with 21400 molecules/cell in log phase SD medium. Ref.8

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Coiled coil Signal-anchor Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein folding Inferred from mutant phenotype. Source: SGD protein localization at cell surface Inferred from mutant phenotype Ref.6. Source: SGD
Cellular component	integral to endoplasmic reticulum membrane Inferred from direct assay Ref.6. Source: SGD mitochondrial outer membrane Inferred from direct assay. Source: SGD
Molecular function	unfolded protein binding Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 403	403	Glucose-signaling factor 2		PRO_0000083865
Regions
	Topological domain	1 – 177	177	Lumenal Potential
	Transmembrane	178 – 198	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	199 – 403	205	Cytoplasmic Potential
	Coiled coil	330 – 388	59	Potential
Amino acid modifications
	Glycosylation	89	1	N-linked (GlcNAc...) Potential
	Glycosylation	173	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q04697 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 096E07AFFA711ECB	FASTA	403	45,870
	10 20 30 40 50 60 MEIYIRLNAD VEHDYAFQVS NEDTINNKIK KIFPSKTGLA DLMVLRPSIF HEKEPVKFYK 70 80 90 100 110 120 SIHPGYLSEG GCLMFHYEAD NEENLEELND SKPLIDQLWP GQLVVPEWKL SKKNIWVYTI 130 140 150 160 170 180 IMLAWLYTDL PDAISPTPGI CLTNQLSRLL IPVAKHMDLP EIAAKLEQEV QANYSSLVAQ 190 200 210 220 230 240 WLFFVMHIFK VGIITLFLKL GIANPISFNP YKLWSLRDLT SPSANGAKNS GGNNNTTDLK 250 260 270 280 290 300 TRLRSLGWIG AKRATYDDYQ TNYYNYVIDK MGGAVAAYRA GAIRKAAAPG IQLVAGEGFQ 310 320 330 340 350 360 SPLEDRFTAS TFTAIKTERK FILSEEYFVE LENNLKKILE EYDGDIGKMN AEIRRFRRFG 370 380 390 400 IYEPDEKLAS LVKLRREIAD EKEKASNNDA TFGIKKNDLK KSN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae." Lussier M., White A.-M., Sheraton J., di Paolo T., Treadwell J., Southard S.B., Horenstein C.I., Chen-Weiner J., Ram A.F.J., Kapteyn J.C., Roemer T.W., Vo D.H., Bondoc D.C., Hall J., Zhong W.-W., Sdicu A.-M., Davies J., Klis F.M., Robbins P.W., Bussey H. Genetics 147:435-450(1997) [PubMed: 9335584] [Abstract] Cited for: IDENTIFICATION.
[5]	"Mutations in GSF1 and GSF2 alter glucose signaling in Saccharomyces cerevisiae." Sherwood P.W., Carlson M. Genetics 147:557-566(1997) [PubMed: 9335593] [Abstract] Cited for: FUNCTION.
[6]	"Efficient export of the glucose transporter Hxt1p from the endoplasmic reticulum requires Gsf2p." Sherwood P.W., Carlson M. Proc. Natl. Acad. Sci. U.S.A. 96:7415-7420(1999) [PubMed: 10377429] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z47816 Genomic DNA. Translation: CAA87826.1. AY692925 Genomic DNA. Translation: AAT92944.1. BK006946 Genomic DNA. Translation: DAA09851.1.
PIR	S50944.
RefSeq	NP_013664.1. NM_001182405.1.
3D structure databases
ProteinModelPortal	Q04697.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4541N.
IntAct	Q04697. 22 interactions.
MINT	MINT-566280.
STRING	Q04697.
Proteomic databases
PeptideAtlas	Q04697.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML048W; YML048W; YML048W.
GeneID	854957.
KEGG	sce:YML048W.
NMPDR	fig|4932.3.peg.4703.
Organism-specific databases
CYGD	YML048w.
SGD	S000004511. GSF2.
Phylogenomic databases
eggNOG	fuNOG05239.
HOGENOM	HBG398336.
OMA	LYTDLPD.
OrthoDB	EOG4RR9S9.
Gene expression databases
ArrayExpress	Q04697.
Genevestigator	Q04697.
GermOnline	YML048W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR022757. Gsf2. [Graphical view]
Pfam	PF11055. Gsf2. 1 hit. [Graphical view]
ProDom	PD139196. Gsf2. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...
Other
NextBio	978036.

Entry information

Entry name

GSF2_YEAST

Accession

Primary (citable) accession number: Q04697
Secondary accession number(s): D6VZC7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	December 14, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program