Glucose-signaling factor 2 - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot Q04697 (GSF2_YEAST)

Last modified June 16, 2009. Version 60. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Glucose-signaling factor 2
Alternative name(s):
Extracellular mutant protein 6

Gene names

Name:	GSF2
Synonyms:	ECM6, EFF2
Ordered Locus Names:	YML048W
ORF Names:	YM9827.04

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	May be involved in the secretion of hexose transporters from the endoplasmic reticulum. Involved in secretion of GAL2 and HXT1. Ref.4 Ref.5
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein Potential.
Miscellaneous	Present with 21400 molecules/cell in log phase SD medium. Ref.7

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Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Coiled coil Signal-anchor Transmembrane
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein folding Inferred from mutant phenotype. Source: SGD protein localization at cell surface Ref.5 Inferred from genetic interaction. Source: SGD
Cellular component	integral to endoplasmic reticulum membrane Ref.5 Inferred from direct assay. Source: SGD mitochondrial outer membrane Inferred from direct assay. Source: SGD
Molecular function	unfolded protein binding Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
ERG11	P10614	1	EBI-27807,EBI-5127
ERP1	Q05359	1	EBI-27807,EBI-6581
ERV25	P54837	1	EBI-27807,EBI-6642
ERV29	P53337	1	EBI-27807,EBI-23662
GAP1	P19145	1	EBI-27807,EBI-7314
HAP3	P13434	1	EBI-27807,EBI-8157
OLE1	P21147	1	EBI-27807,EBI-2098
ORM2	Q06144	1	EBI-27807,EBI-34916
PHO88	P38264	1	EBI-27807,EBI-13350
PIS1	P06197	1	EBI-27807,EBI-13458
PRP40	P33203	1	EBI-27807,EBI-701
RSP5	P39940	1	EBI-27807,EBI-16219
SNL1	P40548	1	EBI-27807,EBI-24950
SPC1	P46965	1	EBI-27807,EBI-17823
SSM4	P40318	1	EBI-27807,EBI-18208
SWP1	Q02795	1	EBI-27807,EBI-12666

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 403

403

Glucose-signaling factor 2

PRO_0000083865

Regions

Topological domain

1 – 177

177

Lumenal Potential

Transmembrane

178 – 198

Signal-anchor for type II membrane protein Potential

Topological domain

199 – 403

205

Cytoplasmic Potential

Coiled coil

330 – 388

Potential

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

173

N-linked (GlcNAc...) Potential

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Sequences

Sequence

Length

Mass (Da)

Tools

Q04697-1 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 096E07AFFA711ECB
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FASTA

403

45,870

        10         20         30         40         50         60 
MEIYIRLNAD VEHDYAFQVS NEDTINNKIK KIFPSKTGLA DLMVLRPSIF HEKEPVKFYK 

        70         80         90        100        110        120 
SIHPGYLSEG GCLMFHYEAD NEENLEELND SKPLIDQLWP GQLVVPEWKL SKKNIWVYTI 

       130        140        150        160        170        180 
IMLAWLYTDL PDAISPTPGI CLTNQLSRLL IPVAKHMDLP EIAAKLEQEV QANYSSLVAQ 

       190        200        210        220        230        240 
WLFFVMHIFK VGIITLFLKL GIANPISFNP YKLWSLRDLT SPSANGAKNS GGNNNTTDLK 

       250        260        270        280        290        300 
TRLRSLGWIG AKRATYDDYQ TNYYNYVIDK MGGAVAAYRA GAIRKAAAPG IQLVAGEGFQ 

       310        320        330        340        350        360 
SPLEDRFTAS TFTAIKTERK FILSEEYFVE LENNLKKILE EYDGDIGKMN AEIRRFRRFG 

       370        380        390        400 
IYEPDEKLAS LVKLRREIAD EKEKASNNDA TFGIKKNDLK KSN

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae." Lussier M., White A.-M., Sheraton J., di Paolo T., Treadwell J., Southard S.B., Horenstein C.I., Chen-Weiner J., Ram A.F.J., Kapteyn J.C., Roemer T.W., Vo D.H., Bondoc D.C., Hall J., Zhong W.-W., Sdicu A.-M., Davies J., Klis F.M., Robbins P.W., Bussey H. Genetics 147:435-450(1997) [PubMed: 9335584] [Abstract] Cited for: IDENTIFICATION.
[4]	"Mutations in GSF1 and GSF2 alter glucose signaling in Saccharomyces cerevisiae." Sherwood P.W., Carlson M. Genetics 147:557-566(1997) [PubMed: 9335593] [Abstract] Cited for: FUNCTION.
[5]	"Efficient export of the glucose transporter Hxt1p from the endoplasmic reticulum requires Gsf2p." Sherwood P.W., Carlson M. Proc. Natl. Acad. Sci. U.S.A. 96:7415-7420(1999) [PubMed: 10377429] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	Z47816 Genomic DNA. Translation: CAA87826.1. AY692925 Genomic DNA. Translation: AAT92944.1.
PIR	S50944.
RefSeq	NP_013664.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4541N.
IntAct	Q04697. 28 interactions.
Proteomic databases
PeptideAtlas	Q04697.
Genome annotation databases
Ensembl	YML048W. Saccharomyces cerevisiae. [Contig view]
GeneID	854957.
GenomeReviews	Gene locus YML048W in contig Z71257_GR.
KEGG	sce:YML048W.
NMPDR	fig\|4932.3.peg.4703.
Organism-specific databases
CYGD	YML048w.
SGD	S000004511. GSF2.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q04697.
OMA	Q04697. LYTDLPD.
Gene expression databases
ArrayExpress	Q04697.
GermOnline	YML048W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	978036.

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Entry information

Entry name

GSF2_YEAST

Accession

Primary (citable) accession number: Q04697

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents