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Protein

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1

Gene

Smarcad1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity and is both required for DNA repair and heterochromatin organization. Promotes DNA end resection of double-strand breaks (DSBs) following DNA damage: probably acts by weakening histone DNA interactions in nucleosomes flanking DSBs. Required for the restoration of heterochromatin organization after replication. Acts at replication sites to facilitate the maintenance of heterochromatin by directing H3 and H4 histones deacetylation, H3 'Lys-9' trimethylation (H3K9me3) and restoration of silencing (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi516 – 524ATPPROSITE-ProRule annotation9
Nucleotide bindingi892 – 899ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, DNA-binding, Helicase, Hydrolase
Biological processDNA damage, DNA repair
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1 (EC:3.6.4.12)
Alternative name(s):
ATP-dependent helicase SMARCAD1
Enhancer trap locus homolog 1
Short name:
Etl-1
Gene namesi
Name:Smarcad1
Synonyms:Etl1, Kiaa1122
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:95453 Smarcad1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Disruption phenotypei

Deficient mice have reduced viability and show growth retardation, skeletal dysplasia and impaired fertility.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000743571 – 1021SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1Add BLAST1021

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei54PhosphothreonineBy similarity1
Modified residuei57PhosphoserineBy similarity1
Cross-linki77Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei79PhosphoserineBy similarity1
Modified residuei124PhosphoserineCombined sources1
Modified residuei127PhosphoserineCombined sources1
Modified residuei132PhosphoserineBy similarity1
Modified residuei144PhosphoserineCombined sources1
Modified residuei145PhosphoserineCombined sources1
Modified residuei151PhosphoserineCombined sources1
Modified residuei210PhosphoserineBy similarity1
Modified residuei213PhosphoserineBy similarity1
Modified residuei235PhosphoserineBy similarity1
Modified residuei238PhosphoserineBy similarity1
Modified residuei298PhosphoserineBy similarity1
Cross-linki330Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki466Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki719Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki991Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ04692
MaxQBiQ04692
PaxDbiQ04692
PeptideAtlasiQ04692
PRIDEiQ04692

PTM databases

iPTMnetiQ04692
PhosphoSitePlusiQ04692

Expressioni

Developmental stagei

Detected at low levels in fertilized and unfertilized eggs. Levels increased in two-cell embryos, decreased up to morula stage and were highest in blastocysts. Highly expressed in the inner cell mass of 3.5 day old blastocysts. Highly expressed in ectoderm and visceral endoderm at day 5.5. Detected throughout the brain and spinal cord at day 10 to 15. Detected in the basal layer of the epidermis after day 12.5, in particular on snout and distal on fore- and hindlimbs.1 Publication

Gene expression databases

BgeeiENSMUSG00000029920
ExpressionAtlasiQ04692 baseline and differential
GenevisibleiQ04692 MM

Interactioni

Subunit structurei

Binds to DNA preferentially in the vicinity of transcriptional start sites. Interacts with MSH2 and TRIM28. Part of a complex composed of TRIM28, HDAC1, HDAC2 and EHMT2. Interacts with PCNA (By similarity).By similarity

Protein-protein interaction databases

BioGridi199524, 3 interactors
STRINGi10090.ENSMUSP00000031984

Structurei

3D structure databases

ProteinModelPortaliQ04692
SMRiQ04692
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini156 – 198CUE 1PROSITE-ProRule annotationAdd BLAST43
Domaini247 – 290CUE 2PROSITE-ProRule annotationAdd BLAST44
Domaini504 – 672Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST169
Domaini853 – 1005Helicase C-terminalPROSITE-ProRule annotationAdd BLAST153

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi623 – 626DEGH box4
Motifi716 – 733Nuclear localization signalSequence analysisAdd BLAST18
Motifi1000 – 1003DEAD box4

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0389 Eukaryota
ENOG410XNUT LUCA
GeneTreeiENSGT00910000144252
HOGENOMiHOG000172362
HOVERGENiHBG055804
InParanoidiQ04692
KOiK14439
OMAiKEHEWMY
OrthoDBiEOG091G0307
PhylomeDBiQ04692
TreeFamiTF105768

Family and domain databases

CDDicd00079 HELICc, 1 hit
Gene3Di3.40.50.10810, 1 hit
InterProiView protein in InterPro
IPR003892 CUE
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR038718 SNF2-like_sf
IPR000330 SNF2_N
PfamiView protein in Pfam
PF00271 Helicase_C, 1 hit
PF00176 SNF2_N, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 3 hits
PROSITEiView protein in PROSITE
PS51140 CUE, 2 hits
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q04692-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNLFNLDRFR FEKRSKIEEA PEAAPQPSQA RPSSPISLSA EEENAEGEGS
60 70 80 90 100
RANTPDSDVT EKTEDSSVPE PPDNERKASL SCFQNQRAIQ EYIDLSSDTE
110 120 130 140 150
DVSPNCSSTV QEKKFSKDTV IIVSEPSEDE ESHDLPSVTR RNDSSELEDL
160 170 180 190 200
SELEDLKDAK LQTLKELFPQ RSDSDLLKLI ESTSTMDGAI AAALLMFGDA
210 220 230 240 250
GGGPRKRKLS SSSEEDDVND DQSVKQPRGD RGEESNESAE ASSNWEKQES
260 270 280 290 300
IVLKLQKEFP NFDKQELREV LKEHEWMYTE ALESLKVFAE DQDVQCASQS
310 320 330 340 350
EVTNGKEVAR NQNYSKNATK IKMKQKISVK PQNGFNKKRK KNVFNPKKAV
360 370 380 390 400
EDSEYDSGSD AGSSLDEDYS SCEEVMEDGY KGKILHFLQV SSIAELTLIP
410 420 430 440 450
KCSQKKAQKI TELRPFNNWE ALFTKMSKIN GLSEDLIWNC KTVIQERDVV
460 470 480 490 500
IRLMNKCEDI SNKLTKQVTM LTGNGGGWNR EQPSLLNQSL SLKPYQKVGL
510 520 530 540 550
NWLALVHKHG LNGILADEMG LGKTIQAIAF LAYLFQEGNK GPHLIVVPAS
560 570 580 590 600
TIDNWLREVN LWCPSLNVLC YYGSQEERKQ IRFNIHNKYE DYNVIVTTYN
610 620 630 640 650
CAISSSDDRS LFRRLKLNYA IFDEGHMLKN MGSIRYQHLM TINARNRLLL
660 670 680 690 700
TGTPVQNNLL ELMSLLNFVM PHMFSSSTSE IRRMFSSKTK PADEQSIYEK
710 720 730 740 750
ERIAHAKQII KPFILRRVKE EVLKLLPPKK DRIELCAMSE KQEQLYSGLF
760 770 780 790 800
NRLKKSINNL EKNTEMCNVM MQLRKMANHP LLHRQYYTPE KLKEMSQLML
810 820 830 840 850
KEPTHCEANP DLIFEDMEVM TDFELHVLCK QYQHINSYQL DMDLILDSGK
860 870 880 890 900
FRALGCILSE LKQKGDRVVL FSQFTMMLDI LEVLLKHHQH RYLRLDGKTQ
910 920 930 940 950
ISERIHLIDE FNTDMDIFVF LLSTKAGGLG INLTSANVVI LHDIDCNPYN
960 970 980 990 1000
DKQAEDRCHR VGQTKEVLVI KLISQGTIEE SMLKINQQKL KLEQDMTTVD
1010 1020
EADEGSMPAD IATLLKTSMG L
Length:1,021
Mass (Da):116,451
Last modified:March 28, 2003 - v2
Checksum:iE3237AA2B135538A
GO
Isoform 2 (identifier: Q04692-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-185: Missing.

Note: No experimental confirmation available.
Show »
Length:836
Mass (Da):95,787
Checksum:iDD7B56518C73242B
GO

Sequence cautioni

The sequence BAC65736 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA49560 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti857I → S in CAA49560 (PubMed:1489724).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0070801 – 185Missing in isoform 2. 1 PublicationAdd BLAST185

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69942 mRNA Translation: CAA49560.1 Different initiation.
AK122454 mRNA Translation: BAC65736.1 Different initiation.
BC042442 mRNA Translation: AAH42442.1
AK134442 mRNA Translation: BAE22146.1
AK147884 mRNA Translation: BAE28202.1
CCDSiCCDS20204.1 [Q04692-1]
PIRiA56559
RefSeqiNP_001240321.1, NM_001253392.1 [Q04692-2]
NP_031984.1, NM_007958.1 [Q04692-1]
XP_006505572.1, XM_006505509.3
XP_006505573.1, XM_006505510.2 [Q04692-1]
XP_006505575.1, XM_006505512.3 [Q04692-2]
XP_011239507.1, XM_011241205.2 [Q04692-2]
UniGeneiMm.99113

Genome annotation databases

EnsembliENSMUST00000031984; ENSMUSP00000031984; ENSMUSG00000029920 [Q04692-1]
GeneIDi13990
KEGGimmu:13990
UCSCiuc009ced.1 mouse [Q04692-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSMRCD_MOUSE
AccessioniPrimary (citable) accession number: Q04692
Secondary accession number(s): Q3UGK6, Q3UYR6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: March 28, 2003
Last modified: April 25, 2018
This is version 156 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health