Q04675 (SEN15_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA-splicing endonuclease subunit SEN15

Alternative name(s):
Splicing endonuclease of 15 kDa
tRNA-intron endonuclease SEN15

Gene names

Name:	SEN15
Ordered Locus Names:	YMR059W
ORF Names:	YM9796.12

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	128 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body.
Subunit structure	tRNA splicing endonuclease is a heterotetramer composed of SEN2, SEN15, SEN34 and SEN54. Interacts directly with SEN34. Ref.3
Subcellular location	Nucleus. Endomembrane system; Peripheral membrane protein. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Note: The tRNA splicing endonuclease complex is predominantly associated with the outer membrane of mitochondria, suggesting that tRNA splicing mainly takes place on the mitochondrial surface. Ref.4
Miscellaneous	The tRNA splicing endonuclease complex is present with 100 molecules/cell.
Sequence similarities	Belongs to the SEN15 family.
Sequence caution	The sequence CAA89769.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane Nucleus
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	tRNA-type intron splice site recognition and cleavage Inferred from direct assay Ref.3. Source: SGD
Cellular component	endomembrane system Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell tRNA-intron endonuclease complex Inferred from direct assay Ref.3. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 128	128	tRNA-splicing endonuclease subunit SEN15		PRO_0000194028

Sequences

	Sequence		Length	Mass (Da)
	Q04675 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: 17DF8B5F3824DF82	FASTA	128	14,890
	10 20 30 40 50 60 MATTDIISLV KNNLLYFQMW TEVEILQDDL SWKGNSLRLL RGRPPHKLSN DVDTEHENSL 70 80 90 100 110 120 SSPRPLEFIL PINMSQYKEN FLTLECLSQT FTHLCSPSTE RILLAIINDD GTIVYYFVYK GVRKPKRN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"The yeast tRNA splicing endonuclease: a tetrameric enzyme with two active site subunits homologous to the archaeal tRNA endonucleases." Trotta C.R., Miao F., Arn E.A., Stevens S.W., Ho C.K., Rauhut R., Abelson J.N. Cell 89:849-858(1997) [PubMed: 9200603] [Abstract] Cited for: CHARACTERIZATION, PROTEIN SEQUENCE OF 1-11, SUBUNIT.
[4]	"Possibility of cytoplasmic pre-tRNA splicing: the yeast tRNA splicing endonuclease mainly localizes on the mitochondria." Yoshihisa T., Yunoki-Esaki K., Ohshima C., Tanaka N., Endo T. Mol. Biol. Cell 14:3266-3279(2003) [PubMed: 12925762] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49703 Genomic DNA. Translation: CAA89769.1. Different initiation. BK006946 Genomic DNA. Translation: DAA09957.1.
RefSeq	NP_013775.2. NM_001182557.1.
3D structure databases
ProteinModelPortal	Q04675.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5802N.
IntAct	Q04675. 58 interactions.
MINT	MINT-672948.
STRING	Q04675.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR059W; YMR059W; YMR059W.
GeneID	855081.
KEGG	sce:YMR059W.
NMPDR	fig|4932.3.peg.4822.
Organism-specific databases
CYGD	YMR059w.
SGD	S000004663. SEN15.
Phylogenomic databases
eggNOG	fuNOG12772.
HOGENOM	HBG396040.
OMA	PPHKLSN.
OrthoDB	EOG4CNV1X.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-16149.
Gene expression databases
ArrayExpress	Q04675.
Genevestigator	Q04675.
GermOnline	YMR059W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR018593. tRNA-endonuc_su_Sen15. [Graphical view]
KO	K15325.
Pfam	PF09631. Sen15. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978366.

Entry information

Entry name

SEN15_YEAST

Accession

Primary (citable) accession number: Q04675
Secondary accession number(s): D6VZN3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 15, 1998
Last modified:	December 14, 2011
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program