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Q04675 (SEN15_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-splicing endonuclease subunit SEN15
Alternative name(s):
Splicing endonuclease of 15 kDa
tRNA-intron endonuclease SEN15
Gene names
Name:SEN15
Ordered Locus Names:YMR059W
ORF Names:YM9796.12
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length128 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body.

Subunit structure

tRNA splicing endonuclease is a heterotetramer composed of SEN2, SEN15, SEN34 and SEN54. Interacts directly with SEN34. Ref.3

Subcellular location

Nucleus. Endomembrane system; Peripheral membrane protein. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Note: The tRNA splicing endonuclease complex is predominantly associated with the outer membrane of mitochondria, suggesting that tRNA splicing mainly takes place on the mitochondrial surface. Ref.4

Miscellaneous

The tRNA splicing endonuclease complex is present with 100 molecules/cell.

Sequence similarities

Belongs to the SEN15 family.

Sequence caution

The sequence CAA89769.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 128128tRNA-splicing endonuclease subunit SEN15
PRO_0000194028

Sequences

Sequence LengthMass (Da)Tools
Q04675 [UniParc].

Last modified December 15, 1998. Version 2.
Checksum: 17DF8B5F3824DF82

FASTA12814,890
        10         20         30         40         50         60 
MATTDIISLV KNNLLYFQMW TEVEILQDDL SWKGNSLRLL RGRPPHKLSN DVDTEHENSL 

        70         80         90        100        110        120 
SSPRPLEFIL PINMSQYKEN FLTLECLSQT FTHLCSPSTE RILLAIINDD GTIVYYFVYK 


GVRKPKRN 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"The yeast tRNA splicing endonuclease: a tetrameric enzyme with two active site subunits homologous to the archaeal tRNA endonucleases."
Trotta C.R., Miao F., Arn E.A., Stevens S.W., Ho C.K., Rauhut R., Abelson J.N.
Cell 89:849-858(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, PROTEIN SEQUENCE OF 1-11, SUBUNIT.
[4]"Possibility of cytoplasmic pre-tRNA splicing: the yeast tRNA splicing endonuclease mainly localizes on the mitochondria."
Yoshihisa T., Yunoki-Esaki K., Ohshima C., Tanaka N., Endo T.
Mol. Biol. Cell 14:3266-3279(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49703 Genomic DNA. Translation: CAA89769.1. Different initiation.
BK006946 Genomic DNA. Translation: DAA09957.1.
RefSeqNP_013775.2. NM_001182557.1.

3D structure databases

ProteinModelPortalQ04675.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35234. 72 interactions.
DIPDIP-5802N.
IntActQ04675. 4 interactions.
MINTMINT-672948.
STRING4932.YMR059W.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR059W; YMR059W; YMR059W.
GeneID855081.
KEGGsce:YMR059W.

Organism-specific databases

CYGDYMR059w.
SGDS000004663. SEN15.

Phylogenomic databases

eggNOGNOG73657.
HOGENOMHOG000248579.
KOK15325.
OMAVESHEIL.
OrthoDBEOG7QRR6S.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-16149.
YEAST:G3O-32762-MONOMER.

Gene expression databases

GenevestigatorQ04675.

Family and domain databases

InterProIPR018593. tRNA-endonuc_su_Sen15.
IPR006677. tRNA_intron_Endonuc_cat-like.
[Graphical view]
PfamPF09631. Sen15. 1 hit.
[Graphical view]
SUPFAMSSF53032. SSF53032. 1 hit.
ProtoNetSearch...

Other

NextBio978366.

Entry information

Entry nameSEN15_YEAST
AccessionPrimary (citable) accession number: Q04675
Secondary accession number(s): D6VZN3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 15, 1998
Last modified: April 16, 2014
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families