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Protein

Transcription factor HES-1

Gene

Hes1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity. May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage (By similarity).By similarity1 Publication

GO - Molecular functioni

  • chromatin binding Source: RGD
  • DNA binding Source: RGD
  • E-box binding Source: RGD
  • JUN kinase binding Source: RGD
  • N-box binding Source: UniProtKB
  • protein complex binding Source: RGD
  • protein homodimerization activity Source: UniProtKB
  • sequence-specific DNA binding Source: BHF-UCL
  • transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: UniProtKB
  • transcription factor binding Source: RGD

GO - Biological processi

  • cellular response to fatty acid Source: RGD
  • cellular response to interleukin-1 Source: RGD
  • cellular response to nerve growth factor stimulus Source: RGD
  • cellular response to tumor necrosis factor Source: RGD
  • forebrain radial glial cell differentiation Source: UniProtKB
  • hair cell differentiation Source: RGD
  • negative regulation of calcium ion import Source: RGD
  • negative regulation of forebrain neuron differentiation Source: UniProtKB
  • negative regulation of gene expression Source: RGD
  • negative regulation of glial cell proliferation Source: UniProtKB
  • negative regulation of neurogenesis Source: RGD
  • negative regulation of neuron projection development Source: RGD
  • negative regulation of oligodendrocyte differentiation Source: UniProtKB
  • negative regulation of pro-B cell differentiation Source: UniProtKB
  • negative regulation of RNA polymerase II regulatory region sequence-specific DNA binding Source: RGD
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • neuronal stem cell population maintenance Source: UniProtKB
  • neuron differentiation Source: RGD
  • positive regulation of astrocyte differentiation Source: UniProtKB
  • positive regulation of DNA binding Source: UniProtKB
  • positive regulation of gene expression Source: RGD
  • positive regulation of glial cell differentiation Source: RGD
  • positive regulation of JAK-STAT cascade Source: UniProtKB
  • positive regulation of tyrosine phosphorylation of Stat3 protein Source: UniProtKB
  • protein complex assembly Source: UniProtKB
  • regulation of protein heterodimerization activity Source: RGD
  • response to alkaloid Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to thyroid hormone Source: RGD
  • STAT protein import into nucleus Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor HES-1
Alternative name(s):
Hairy and enhancer of split 1
Hairy-like protein
RHL
Gene namesi
Name:Hes1
Synonyms:Hes-1, Hl
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi62081. Hes1.

Subcellular locationi

GO - Cellular componenti

  • chromatin Source: RGD
  • nuclear matrix Source: RGD
  • nucleoplasm Source: Reactome
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 281281Transcription factor HES-1PRO_0000127204Add
BLAST

Proteomic databases

PaxDbiQ04666.

PTM databases

iPTMnetiQ04666.
PhosphoSiteiQ04666.

Expressioni

Tissue specificityi

Present in all tissues examined but highest in epithelial cells and in mesoderm-derived tissues such as embryonal muscle cells.

Interactioni

Subunit structurei

Interacts with SIRT1. Interacts weakly with TLE2. Interacts with HES6 (By similarity). Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family. Interacts (via WPRW motif) with TLE1. Interacts with an FA complex, composed of FANCA, FANCF, FANCG and FANCL, but not of FANCC, nor FANCE (By similarity).By similarity

GO - Molecular functioni

  • JUN kinase binding Source: RGD
  • protein complex binding Source: RGD
  • protein homodimerization activity Source: UniProtKB
  • transcription factor binding Source: RGD

Protein-protein interaction databases

BioGridi248210. 7 interactions.
IntActiQ04666. 3 interactions.
MINTiMINT-8176807.
STRINGi10116.ENSRNOP00000002346.

Structurei

3D structure databases

ProteinModelPortaliQ04666.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini34 – 9158bHLHPROSITE-ProRule annotationAdd
BLAST
Domaini110 – 14334OrangePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi276 – 2794WRPW motif

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi156 – 24792Pro-richAdd
BLAST
Compositional biasi250 – 27425Ser/Thr-richAdd
BLAST

Domaini

Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).1 Publication
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.1 Publication
The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity.By similarity

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation
Contains 1 Orange domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4304. Eukaryota.
ENOG4111F0X. LUCA.
HOVERGENiHBG005960.
InParanoidiQ04666.
KOiK06054.
PhylomeDBiQ04666.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR003650. Orange_dom.
[Graphical view]
PfamiPF07527. Hairy_orange. 1 hit.
PF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
SM00511. ORANGE. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
PS51054. ORANGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04666-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPADIMEKNS SSPVAATPAS VNTTPDKPKT ASEHRKSSKP IMEKRRRARI
60 70 80 90 100
NESLSQLKTL ILDALKKDSS RHSKLEKADI LEMTVKHLRN LQRAQMTAAL
110 120 130 140 150
STDPSVLGKY RAGFSECMNE VTRFLSTCEG VNTEVRTRLL GHLANCMTQI
160 170 180 190 200
NAMTYPGQAH PALQAPPPPP PSGPGGPQHA PFAPPPPLVP IPGGAAPPPG
210 220 230 240 250
SAPCKLGSQA GEAAKVFGGF QVVPAPDGQF AFLIPNGAFA HSGPVIPVYT
260 270 280
SNSGTSVGPN AVSPSSGSSL TADSMWRPWR N
Length:281
Mass (Da):29,622
Last modified:June 1, 1994 - v1
Checksum:i8A98C8964F075B0D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti86 – 861K → N in AAA41307 (PubMed:8417318).Curated
Sequence conflicti93 – 931R → W in AAA41307 (PubMed:8417318).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13417 mRNA. Translation: BAA02682.1.
L04527 mRNA. Translation: AAA41307.1.
BC061730 mRNA. Translation: AAH61730.1.
PIRiS36748.
RefSeqiNP_077336.3. NM_024360.3.
UniGeneiRn.19727.

Genome annotation databases

GeneIDi29577.
KEGGirno:29577.
UCSCiRGD:62081. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13417 mRNA. Translation: BAA02682.1.
L04527 mRNA. Translation: AAA41307.1.
BC061730 mRNA. Translation: AAH61730.1.
PIRiS36748.
RefSeqiNP_077336.3. NM_024360.3.
UniGeneiRn.19727.

3D structure databases

ProteinModelPortaliQ04666.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248210. 7 interactions.
IntActiQ04666. 3 interactions.
MINTiMINT-8176807.
STRINGi10116.ENSRNOP00000002346.

PTM databases

iPTMnetiQ04666.
PhosphoSiteiQ04666.

Proteomic databases

PaxDbiQ04666.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29577.
KEGGirno:29577.
UCSCiRGD:62081. rat.

Organism-specific databases

CTDi3280.
RGDi62081. Hes1.

Phylogenomic databases

eggNOGiKOG4304. Eukaryota.
ENOG4111F0X. LUCA.
HOVERGENiHBG005960.
InParanoidiQ04666.
KOiK06054.
PhylomeDBiQ04666.

Miscellaneous databases

PROiQ04666.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR003650. Orange_dom.
[Graphical view]
PfamiPF07527. Hairy_orange. 1 hit.
PF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
SM00511. ORANGE. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
PS51054. ORANGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHES1_RAT
AccessioniPrimary (citable) accession number: Q04666
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 8, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.