Q04663 (CPSD_STRA3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein kinase CpsD
EC=2.7.10.2

Gene names

Name:	cpsD
Ordered Locus Names:	gbs1245

Organism

Streptococcus agalactiae serotype III (strain NEM316) [Complete proteome] [HAMAP]

Taxonomic identifier

211110 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus agalactiae › Streptococcus agalactiae serogroup III

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the regulation of capsular polysaccharide biosynthesis. Autophosphorylation of CpsD attenuates its activity and reduces the level of encapsulation. May be part of a complex that directs the coordinated polymerization and export to the cell surface of the capsular polysaccharide By similarity.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Enzyme regulation	Dephosphorylated and activated by CpsB By similarity.
Pathway	Capsule biogenesis; capsule polysaccharide biosynthesis.
Post-translational modification	Autophosphorylated By similarity.
Sequence similarities	Belongs to the CpsD/CapB family.
Caution	Was originally (Ref.1) called CpsC.

Ontologies

Keywords
Biological process	Capsule biogenesis/degradation Exopolysaccharide synthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	capsule polysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of catalytic activity Inferred from electronic annotation. Source: GOC
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW enzyme regulator activity Inferred from electronic annotation. Source: InterPro non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 232	232	Tyrosine-protein kinase CpsD		PRO_0000217235
Experimental info
	Sequence conflict	41	1	T → A in AAB00363. Ref.1
	Sequence conflict	218 – 220	3	Missing in AAB00363. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q04663 [UniParc]. Last modified January 17, 2003. Version 3. Checksum: 7F81FA15A9C77C33	FASTA	232	25,429
	10 20 30 40 50 60 MTRLEIVDSK LRQAKKTEEY FNAIRTNIQF SGKENKILAI TSVREGEGKS TTSTSLALSL 70 80 90 100 110 120 AQAGFKTLLI DADTRNSVMS GTFKATGTIK GLTNYLSGNA DLGDIICETN VPRLMVVPSG 130 140 150 160 170 180 KVPPNPTALL QNAYFNKMIE AIKNIFDYII IDTPPIGLVV DAAIIANACD GFILVTQAGR 190 200 210 220 230 IKRNYVEKAK EQMEQSGSKF LGIILNKVNE SVATYGDYGD YGNYGKRDRK RK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of cpsD, a gene essential for type III capsule expression in group B streptococci." Rubens C.E., Heggen L.M., Haft R.F., Wessels M.R. Mol. Microbiol. 8:843-855(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: COH1 / Serotype III.
[2]	Yim H. Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Genome sequence of Streptococcus agalactiae, a pathogen causing invasive neonatal disease." Glaser P., Rusniok C., Buchrieser C., Chevalier F., Frangeul L., Msadek T., Zouine M., Couve E., Lalioui L., Poyart C., Trieu-Cuot P., Kunst F. Mol. Microbiol. 45:1499-1513(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NEM316.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF163833 Genomic DNA. Translation: AAB00363.1. AL766849 Genomic DNA. Translation: CAD46904.1.
PIR	S34976. T44642.
RefSeq	NP_735689.1. NC_004368.1.
3D structure databases
ProteinModelPortal	Q04663.
ModBase	Search...
Protein-protein interaction databases
STRING	211110.gbs1245.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAD46904; CAD46904; CAD46904.
GeneID	1030264.
KEGG	san:gbs1244.
PATRIC	19638373. VBIStrAga3577_1275.
Organism-specific databases
GenoList	gbs1245.
CMR	Search...
Phylogenomic databases
eggNOG	COG0489.
HOGENOM	HOG000056186.
OMA	PDEIEMI.
ProtClustDB	CLSK883944.
Enzyme and pathway databases
UniPathway	UPA00934.
Family and domain databases
InterPro	IPR025669. AAA_dom. IPR005702. EPS_synthesis. [Graphical view]
Pfam	PF13614. AAA_31. 1 hit. [Graphical view]
TIGRFAMs	TIGR01007. eps_fam. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CPSD_STRA3

Accession

Primary (citable) accession number: Q04663

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	January 17, 2003
Last modified:	May 1, 2013
This is version 85 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program