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Q04660

- ERB1_YEAST

UniProt

Q04660 - ERB1_YEAST

Protein

Ribosome biogenesis protein ERB1

Gene

ERB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome.2 PublicationsUniRule annotation

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. ribonucleoprotein complex binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: UniProtKB-HAMAP
    2. maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: UniProtKB-HAMAP
    3. ribosomal large subunit biogenesis Source: SGD
    4. rRNA processing Source: SGD

    Keywords - Biological processi

    Ribosome biogenesis, rRNA processing

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32754-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribosome biogenesis protein ERB1UniRule annotation
    Alternative name(s):
    Eukaryotic ribosome biogenesis protein 1UniRule annotation
    Gene namesi
    Name:ERB1UniRule annotation
    Ordered Locus Names:YMR049C
    ORF Names:YM9796.02C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR049c.
    SGDiS000004652. ERB1.

    Subcellular locationi

    Nucleusnucleolus 1 PublicationUniRule annotation. Nucleusnucleoplasm 1 PublicationUniRule annotation

    GO - Cellular componenti

    1. nucleoplasm Source: UniProtKB-SubCell
    2. PeBoW complex Source: SGD
    3. preribosome, large subunit precursor Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 807807Ribosome biogenesis protein ERB1PRO_0000050968Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei23 – 231Phosphoserine3 Publications
    Modified residuei72 – 721Phosphoserine1 Publication
    Modified residuei76 – 761Phosphoserine1 Publication
    Modified residuei146 – 1461Phosphoserine1 Publication
    Modified residuei149 – 1491Phosphoserine1 Publication
    Modified residuei418 – 4181Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ04660.
    PaxDbiQ04660.
    PeptideAtlasiQ04660.

    Expressioni

    Gene expression databases

    GenevestigatoriQ04660.

    Interactioni

    Subunit structurei

    Component of the NOP7 complex, composed of ERB1, NOP7 and YTM1. Within the NOP7 complex ERB1 appears to interact directly with NOP7 and YTM1. The NOP7 complex also associates with the 66S pre-ribosome. Also interacts with NOG1.3 PublicationsUniRule annotation

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    NOG1Q028922EBI-28098,EBI-12105
    YTM1Q120244EBI-28098,EBI-29589

    Protein-protein interaction databases

    BioGridi35223. 128 interactions.
    DIPiDIP-3970N.
    IntActiQ04660. 61 interactions.
    MINTiMINT-575265.
    STRINGi4932.YMR049C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ04660.
    SMRiQ04660. Positions 431-515, 590-805.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati435 – 47440WD 1Add
    BLAST
    Repeati483 – 52341WD 2Add
    BLAST
    Repeati592 – 63443WD 3Add
    BLAST
    Repeati637 – 67539WD 4Add
    BLAST
    Repeati678 – 71740WD 5Add
    BLAST
    Repeati721 – 76040WD 6Add
    BLAST
    Repeati776 – 80732WD 7Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni265 – 383119Required for interaction with NOP7Add
    BLAST
    Regioni383 – 41937Required for interaction with YTM1Add
    BLAST

    Sequence similaritiesi

    Belongs to the WD repeat BOP1/ERB1 family.UniRule annotation
    Contains 7 WD repeats.UniRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    GeneTreeiENSGT00390000018422.
    HOGENOMiHOG000029368.
    KOiK14824.
    OMAiWYEDFPH.
    OrthoDBiEOG70PC60.

    Family and domain databases

    Gene3Di2.130.10.10. 2 hits.
    HAMAPiMF_03027. BOP1.
    InterProiIPR028598. BOP1/Erb1.
    IPR012953. BOP1_N_dom.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF08145. BOP1NT. 1 hit.
    PF00400. WD40. 3 hits.
    [Graphical view]
    SMARTiSM01035. BOP1NT. 1 hit.
    SM00320. WD40. 5 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 2 hits.
    PROSITEiPS00678. WD_REPEATS_1. 1 hit.
    PS50082. WD_REPEATS_2. 2 hits.
    PS50294. WD_REPEATS_REGION. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q04660-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMAKNNKTTE AKMSKKRAAS EESDVEEDED KLLSVDGLID AEASESDEDD    50
    DEYESAVEEK ESSSDKEAQD DSDDDSDAEL NKLLAEEEGD GEEDYDSSEF 100
    SDDTTSLTDR LSGVKLQTIV DPNIYSKYAD GSDRIIKPEI NPVYDSDDSD 150
    AETQNTIGNI PLSAYDEMPH IGYDINGKRI MRPAKGSALD QLLDSIELPE 200
    GWTGLLDKNS GSSLNLTKEE LELISKIQRN EQTDDSINPY EPLIDWFTRH 250
    EEVMPLTAVP EPKRRFVPSK NEAKRVMKIV RAIREGRIIP PKKLKEMKEK 300
    EKIENYQYDL WGDSTETNDH VMHLRAPKLP PPTNEESYNP PEEYLLSPEE 350
    KEAWENTEYS ERERNFIPQK YSALRKVPGY GESIRERFER SLDLYLAPRV 400
    RKNKLNIDPN SLIPELPSPK DLRPFPIRCS TIYAGHKGKV RTLSIDPSGL 450
    WLATGSDDGT VRVWEILTGR EVYRTTLIDD EENPDYHIEC IEWNPDANNG 500
    ILAVAVGENI HLIVPPIFGY DIENNGKTKI EDGFGYDTFG TVKKSNLEVN 550
    ENGDGDEDGE NESAKNAVKK QVAQWNKPSQ KQLEKDICIT ISCKKTVKKL 600
    SWHRKGDYFV TVQPDSGNTS VLIHQVSKHL TQSPFKKSKG IIMDAKFHPF 650
    KPQLFVCSQR YVRIYDLSQQ ILVKKLLPGA RWLSKIDIHP RGDNLIASSF 700
    DKRVLWHDLD LASTPYKTLR YHEKAVRSVN FHKKLPLFSS AADDGTIHVF 750
    HATVYDDMMK NPMIVPLKKL TGHKVINSLG VLDAIWHPRE AWLFSAGADN 800
    TARLWTT 807
    Length:807
    Mass (Da):91,705
    Last modified:November 1, 1997 - v1
    Checksum:i6542DFE4670341CA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49703 Genomic DNA. Translation: CAA89759.1.
    BK006946 Genomic DNA. Translation: DAA09948.1.
    PIRiS54549.
    RefSeqiNP_013764.1. NM_001182546.1.

    Genome annotation databases

    EnsemblFungiiYMR049C; YMR049C; YMR049C.
    GeneIDi855068.
    KEGGisce:YMR049C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49703 Genomic DNA. Translation: CAA89759.1 .
    BK006946 Genomic DNA. Translation: DAA09948.1 .
    PIRi S54549.
    RefSeqi NP_013764.1. NM_001182546.1.

    3D structure databases

    ProteinModelPortali Q04660.
    SMRi Q04660. Positions 431-515, 590-805.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35223. 128 interactions.
    DIPi DIP-3970N.
    IntActi Q04660. 61 interactions.
    MINTi MINT-575265.
    STRINGi 4932.YMR049C.

    Proteomic databases

    MaxQBi Q04660.
    PaxDbi Q04660.
    PeptideAtlasi Q04660.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR049C ; YMR049C ; YMR049C .
    GeneIDi 855068.
    KEGGi sce:YMR049C.

    Organism-specific databases

    CYGDi YMR049c.
    SGDi S000004652. ERB1.

    Phylogenomic databases

    eggNOGi COG2319.
    GeneTreei ENSGT00390000018422.
    HOGENOMi HOG000029368.
    KOi K14824.
    OMAi WYEDFPH.
    OrthoDBi EOG70PC60.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32754-MONOMER.

    Miscellaneous databases

    NextBioi 978335.
    PROi Q04660.

    Gene expression databases

    Genevestigatori Q04660.

    Family and domain databases

    Gene3Di 2.130.10.10. 2 hits.
    HAMAPi MF_03027. BOP1.
    InterProi IPR028598. BOP1/Erb1.
    IPR012953. BOP1_N_dom.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF08145. BOP1NT. 1 hit.
    PF00400. WD40. 3 hits.
    [Graphical view ]
    SMARTi SM01035. BOP1NT. 1 hit.
    SM00320. WD40. 5 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 2 hits.
    PROSITEi PS00678. WD_REPEATS_1. 1 hit.
    PS50082. WD_REPEATS_2. 2 hits.
    PS50294. WD_REPEATS_REGION. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "ERB1, the yeast homolog of mammalian Bop1, is an essential gene required for maturation of the 25S and 5.8S ribosomal RNAs."
      Pestov D.G., Stockelman M.G., Strezoska Z., Lau L.F.
      Nucleic Acids Res. 29:3621-3630(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "Yph1p, an ORC-interacting protein: potential links between cell proliferation control, DNA replication, and ribosome biogenesis."
      Du Y.-C.N., Stillman B.
      Cell 109:835-848(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE NOP7 COMPLEX.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Ytm1, Nop7, and Erb1 form a complex necessary for maturation of yeast 66S preribosomes."
      Miles T.D., Jakovljevic J., Horsey E.W., Harnpicharnchai P., Tang L., Woolford J.L. Jr.
      Mol. Cell. Biol. 25:10419-10432(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE NOP7 COMPLEX, ASSOCIATION OF THE NOP7 COMPLEX WITH 66S PRE-RIBOSOMES.
    7. "TOR regulates late steps of ribosome maturation in the nucleoplasm via Nog1 in response to nutrients."
      Honma Y., Kitamura A., Shioda R., Maruyama H., Ozaki K., Oda Y., Mini T., Jenoe P., Maki Y., Yonezawa K., Hurt E., Ueno M., Uritani M., Hall M.N., Ushimaru T.
      EMBO J. 25:3832-3842(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NOG1.
    8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23 AND SER-418, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    9. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23; SER-72 AND SER-76, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Interactions among Ytm1, Erb1, and Nop7 required for assembly of the Nop7-subcomplex in yeast preribosomes."
      Tang L., Sahasranaman A., Jakovljevic J., Schleifman E., Woolford J.L. Jr.
      Mol. Biol. Cell 19:2844-2856(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CHARACTERIZATION OF THE NOP7 COMPLEX, SUBCELLULAR LOCATION.
    11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23; SER-146; SER-149 AND SER-418, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiERB1_YEAST
    AccessioniPrimary (citable) accession number: Q04660
    Secondary accession number(s): D6VZM4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2680 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3