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Q04660 (ERB1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosome biogenesis protein ERB1
Alternative name(s):
Eukaryotic ribosome biogenesis protein 1
Gene names
Name:ERB1
Ordered Locus Names:YMR049C
ORF Names:YM9796.02C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length807 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome. Ref.3 Ref.10

Subunit structure

Component of the NOP7 complex, composed of ERB1, NOP7 and YTM1. Within the NOP7 complex ERB1 appears to interact directly with NOP7 and YTM1. The NOP7 complex also associates with the 66S pre-ribosome. Also interacts with NOG1. Ref.4 Ref.6 Ref.7

Subcellular location

Nucleusnucleolus. Nucleusnucleoplasm Ref.10.

Miscellaneous

Present with 2680 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the WD repeat BOP1/ERB1 family.

Contains 7 WD repeats.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

NOG1Q028922EBI-28098,EBI-12105
YTM1Q120244EBI-28098,EBI-29589

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 807807Ribosome biogenesis protein ERB1 HAMAP-Rule MF_03027
PRO_0000050968

Regions

Repeat435 – 47440WD 1 HAMAP-Rule MF_03027
Repeat483 – 52341WD 2 HAMAP-Rule MF_03027
Repeat592 – 63443WD 3 HAMAP-Rule MF_03027
Repeat637 – 67539WD 4 HAMAP-Rule MF_03027
Repeat678 – 71740WD 5 HAMAP-Rule MF_03027
Repeat721 – 76040WD 6 HAMAP-Rule MF_03027
Repeat776 – 80732WD 7 HAMAP-Rule MF_03027
Region265 – 383119Required for interaction with NOP7 HAMAP-Rule MF_03027
Region383 – 41937Required for interaction with YTM1 HAMAP-Rule MF_03027

Amino acid modifications

Modified residue231Phosphoserine Ref.8 Ref.9 Ref.11
Modified residue721Phosphoserine Ref.9
Modified residue761Phosphoserine Ref.9
Modified residue1461Phosphoserine Ref.11
Modified residue1491Phosphoserine Ref.11
Modified residue4181Phosphoserine Ref.8 Ref.11

Sequences

Sequence LengthMass (Da)Tools
Q04660 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 6542DFE4670341CA

FASTA80791,705
        10         20         30         40         50         60 
MMAKNNKTTE AKMSKKRAAS EESDVEEDED KLLSVDGLID AEASESDEDD DEYESAVEEK 

        70         80         90        100        110        120 
ESSSDKEAQD DSDDDSDAEL NKLLAEEEGD GEEDYDSSEF SDDTTSLTDR LSGVKLQTIV 

       130        140        150        160        170        180 
DPNIYSKYAD GSDRIIKPEI NPVYDSDDSD AETQNTIGNI PLSAYDEMPH IGYDINGKRI 

       190        200        210        220        230        240 
MRPAKGSALD QLLDSIELPE GWTGLLDKNS GSSLNLTKEE LELISKIQRN EQTDDSINPY 

       250        260        270        280        290        300 
EPLIDWFTRH EEVMPLTAVP EPKRRFVPSK NEAKRVMKIV RAIREGRIIP PKKLKEMKEK 

       310        320        330        340        350        360 
EKIENYQYDL WGDSTETNDH VMHLRAPKLP PPTNEESYNP PEEYLLSPEE KEAWENTEYS 

       370        380        390        400        410        420 
ERERNFIPQK YSALRKVPGY GESIRERFER SLDLYLAPRV RKNKLNIDPN SLIPELPSPK 

       430        440        450        460        470        480 
DLRPFPIRCS TIYAGHKGKV RTLSIDPSGL WLATGSDDGT VRVWEILTGR EVYRTTLIDD 

       490        500        510        520        530        540 
EENPDYHIEC IEWNPDANNG ILAVAVGENI HLIVPPIFGY DIENNGKTKI EDGFGYDTFG 

       550        560        570        580        590        600 
TVKKSNLEVN ENGDGDEDGE NESAKNAVKK QVAQWNKPSQ KQLEKDICIT ISCKKTVKKL 

       610        620        630        640        650        660 
SWHRKGDYFV TVQPDSGNTS VLIHQVSKHL TQSPFKKSKG IIMDAKFHPF KPQLFVCSQR 

       670        680        690        700        710        720 
YVRIYDLSQQ ILVKKLLPGA RWLSKIDIHP RGDNLIASSF DKRVLWHDLD LASTPYKTLR 

       730        740        750        760        770        780 
YHEKAVRSVN FHKKLPLFSS AADDGTIHVF HATVYDDMMK NPMIVPLKKL TGHKVINSLG 

       790        800 
VLDAIWHPRE AWLFSAGADN TARLWTT 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"ERB1, the yeast homolog of mammalian Bop1, is an essential gene required for maturation of the 25S and 5.8S ribosomal RNAs."
Pestov D.G., Stockelman M.G., Strezoska Z., Lau L.F.
Nucleic Acids Res. 29:3621-3630(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Yph1p, an ORC-interacting protein: potential links between cell proliferation control, DNA replication, and ribosome biogenesis."
Du Y.-C.N., Stillman B.
Cell 109:835-848(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE NOP7 COMPLEX.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Ytm1, Nop7, and Erb1 form a complex necessary for maturation of yeast 66S preribosomes."
Miles T.D., Jakovljevic J., Horsey E.W., Harnpicharnchai P., Tang L., Woolford J.L. Jr.
Mol. Cell. Biol. 25:10419-10432(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE NOP7 COMPLEX, ASSOCIATION OF THE NOP7 COMPLEX WITH 66S PRE-RIBOSOMES.
[7]"TOR regulates late steps of ribosome maturation in the nucleoplasm via Nog1 in response to nutrients."
Honma Y., Kitamura A., Shioda R., Maruyama H., Ozaki K., Oda Y., Mini T., Jenoe P., Maki Y., Yonezawa K., Hurt E., Ueno M., Uritani M., Hall M.N., Ushimaru T.
EMBO J. 25:3832-3842(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NOG1.
[8]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23 AND SER-418, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[9]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23; SER-72 AND SER-76, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Interactions among Ytm1, Erb1, and Nop7 required for assembly of the Nop7-subcomplex in yeast preribosomes."
Tang L., Sahasranaman A., Jakovljevic J., Schleifman E., Woolford J.L. Jr.
Mol. Biol. Cell 19:2844-2856(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CHARACTERIZATION OF THE NOP7 COMPLEX, SUBCELLULAR LOCATION.
[11]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23; SER-146; SER-149 AND SER-418, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49703 Genomic DNA. Translation: CAA89759.1.
BK006946 Genomic DNA. Translation: DAA09948.1.
PIRS54549.
RefSeqNP_013764.1. NM_001182546.1.

3D structure databases

ProteinModelPortalQ04660.
SMRQ04660. Positions 431-515, 590-805.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35223. 128 interactions.
DIPDIP-3970N.
IntActQ04660. 61 interactions.
MINTMINT-575265.
STRING4932.YMR049C.

Proteomic databases

MaxQBQ04660.
PaxDbQ04660.
PeptideAtlasQ04660.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR049C; YMR049C; YMR049C.
GeneID855068.
KEGGsce:YMR049C.

Organism-specific databases

CYGDYMR049c.
SGDS000004652. ERB1.

Phylogenomic databases

eggNOGCOG2319.
GeneTreeENSGT00390000018422.
HOGENOMHOG000029368.
KOK14824.
OMAWYEDFPH.
OrthoDBEOG70PC60.

Enzyme and pathway databases

BioCycYEAST:G3O-32754-MONOMER.

Gene expression databases

GenevestigatorQ04660.

Family and domain databases

Gene3D2.130.10.10. 2 hits.
HAMAPMF_03027. BOP1.
InterProIPR028598. BOP1/Erb1.
IPR012953. BOP1_N_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF08145. BOP1NT. 1 hit.
PF00400. WD40. 3 hits.
[Graphical view]
SMARTSM01035. BOP1NT. 1 hit.
SM00320. WD40. 5 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 2 hits.
PROSITEPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio978335.
PROQ04660.

Entry information

Entry nameERB1_YEAST
AccessionPrimary (citable) accession number: Q04660
Secondary accession number(s): D6VZM4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 11, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families