Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ribosome biogenesis protein ERB1

Gene

ERB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome.UniRule annotation2 Publications

GO - Molecular functioni

  • large ribosomal subunit rRNA binding Source: GO_Central
  • ribonucleoprotein complex binding Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-32754-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome biogenesis protein ERB1UniRule annotation
Alternative name(s):
Eukaryotic ribosome biogenesis protein 11 PublicationUniRule annotation
Gene namesi
Name:ERB11 PublicationUniRule annotation
Ordered Locus Names:YMR049CImported
ORF Names:YM9796.02C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR049C.
SGDiS000004652. ERB1.

Subcellular locationi

  • Nucleusnucleolus UniRule annotation1 Publication
  • Nucleusnucleoplasm UniRule annotation1 Publication

GO - Cellular componenti

  • nucleoplasm Source: UniProtKB-SubCell
  • PeBoW complex Source: SGD
  • preribosome, large subunit precursor Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi465E → R: Disrupts the interaction with YTM1 and cannot sustain growth. 2 Publications1
Mutagenesisi470R → E: Weakens the interaction with YTM1, impairs pre-rRNA processing and leads to a deficiency in 60S ribosomal subunits. Disrupts the interaction with YTM1 and cannot sustain growth; when associated with R-790. 2 Publications1
Mutagenesisi790E → R: Disrupts the interaction with YTM1 and cannot sustain growth; when associated with E-470. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000509681 – 807Ribosome biogenesis protein ERB1Add BLAST807

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei23PhosphoserineCombined sources1
Modified residuei72PhosphoserineCombined sources1
Modified residuei76PhosphoserineCombined sources1
Cross-linki127Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei146PhosphoserineCombined sources1
Modified residuei149PhosphoserineCombined sources1
Modified residuei418PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ04660.
PRIDEiQ04660.

PTM databases

iPTMnetiQ04660.

Interactioni

Subunit structurei

Component of the NOP7 complex, composed of ERB1, NOP7 and YTM1. The complex is held together by ERB1, which interacts with NOP7 via its N-terminal domain and with YTM1 via a high-affinity interaction between the seven-bladed beta-propeller domains of the 2 proteins. The NOP7 complex associates with the 66S pre-ribosome. Also interacts with NOG1.UniRule annotation3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DRS1P328924EBI-28098,EBI-6170
HAS1Q035324EBI-28098,EBI-8170
NOC2P397447EBI-28098,EBI-29259
NOG1Q028922EBI-28098,EBI-12105
NOP7P532619EBI-28098,EBI-13145
URB1P342413EBI-28098,EBI-26595
YTM1Q120249EBI-28098,EBI-29589

Protein-protein interaction databases

BioGridi35223. 126 interactors.
DIPiDIP-3970N.
IntActiQ04660. 67 interactors.
MINTiMINT-575265.

Structurei

Secondary structure

1807
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi419 – 422Combined sources4
Beta strandi428 – 433Combined sources6
Beta strandi440 – 445Combined sources6
Beta strandi451 – 456Combined sources6
Beta strandi459 – 465Combined sources7
Turni466 – 468Combined sources3
Beta strandi471 – 476Combined sources6
Beta strandi488 – 493Combined sources6
Beta strandi502 – 506Combined sources5
Beta strandi509 – 513Combined sources5
Helixi520 – 532Combined sources13
Beta strandi573 – 576Combined sources4
Helixi580 – 583Combined sources4
Turni584 – 586Combined sources3
Beta strandi587 – 595Combined sources9
Beta strandi599 – 602Combined sources4
Beta strandi606 – 612Combined sources7
Helixi617 – 619Combined sources3
Beta strandi620 – 625Combined sources6
Helixi626 – 628Combined sources3
Beta strandi630 – 632Combined sources3
Beta strandi642 – 647Combined sources6
Beta strandi649 – 660Combined sources12
Beta strandi662 – 666Combined sources5
Turni667 – 670Combined sources4
Beta strandi671 – 676Combined sources6
Beta strandi683 – 688Combined sources6
Beta strandi692 – 699Combined sources8
Beta strandi704 – 708Combined sources5
Turni709 – 711Combined sources3
Beta strandi716 – 719Combined sources4
Beta strandi726 – 731Combined sources6
Beta strandi733 – 742Combined sources10
Beta strandi747 – 753Combined sources7
Beta strandi756 – 759Combined sources4
Beta strandi763 – 770Combined sources8
Beta strandi781 – 786Combined sources6
Beta strandi788 – 791Combined sources4
Beta strandi793 – 797Combined sources5
Beta strandi802 – 806Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4U7AX-ray1.60A1-807[»]
ProteinModelPortaliQ04660.
SMRiQ04660.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati435 – 474WD 1UniRule annotationAdd BLAST40
Repeati483 – 523WD 2UniRule annotationAdd BLAST41
Repeati592 – 634WD 3UniRule annotationAdd BLAST43
Repeati637 – 675WD 4UniRule annotationAdd BLAST39
Repeati678 – 717WD 5UniRule annotationAdd BLAST40
Repeati721 – 760WD 6UniRule annotationAdd BLAST40
Repeati776 – 807WD 7UniRule annotationAdd BLAST32

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni265 – 383Required for interaction with NOP71 PublicationAdd BLAST119
Regioni383 – 419Required for interaction with YTM11 PublicationAdd BLAST37

Sequence similaritiesi

Belongs to the WD repeat BOP1/ERB1 family.UniRule annotation
Contains 7 WD repeats.UniRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00390000018422.
HOGENOMiHOG000029368.
InParanoidiQ04660.
KOiK14824.
OMAiPTIVPVK.
OrthoDBiEOG092C1381.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
HAMAPiMF_03027. BOP1. 1 hit.
InterProiIPR028598. BOP1/Erb1.
IPR012953. BOP1_N_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08145. BOP1NT. 1 hit.
PF00400. WD40. 3 hits.
[Graphical view]
SMARTiSM01035. BOP1NT. 1 hit.
SM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04660-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMAKNNKTTE AKMSKKRAAS EESDVEEDED KLLSVDGLID AEASESDEDD
60 70 80 90 100
DEYESAVEEK ESSSDKEAQD DSDDDSDAEL NKLLAEEEGD GEEDYDSSEF
110 120 130 140 150
SDDTTSLTDR LSGVKLQTIV DPNIYSKYAD GSDRIIKPEI NPVYDSDDSD
160 170 180 190 200
AETQNTIGNI PLSAYDEMPH IGYDINGKRI MRPAKGSALD QLLDSIELPE
210 220 230 240 250
GWTGLLDKNS GSSLNLTKEE LELISKIQRN EQTDDSINPY EPLIDWFTRH
260 270 280 290 300
EEVMPLTAVP EPKRRFVPSK NEAKRVMKIV RAIREGRIIP PKKLKEMKEK
310 320 330 340 350
EKIENYQYDL WGDSTETNDH VMHLRAPKLP PPTNEESYNP PEEYLLSPEE
360 370 380 390 400
KEAWENTEYS ERERNFIPQK YSALRKVPGY GESIRERFER SLDLYLAPRV
410 420 430 440 450
RKNKLNIDPN SLIPELPSPK DLRPFPIRCS TIYAGHKGKV RTLSIDPSGL
460 470 480 490 500
WLATGSDDGT VRVWEILTGR EVYRTTLIDD EENPDYHIEC IEWNPDANNG
510 520 530 540 550
ILAVAVGENI HLIVPPIFGY DIENNGKTKI EDGFGYDTFG TVKKSNLEVN
560 570 580 590 600
ENGDGDEDGE NESAKNAVKK QVAQWNKPSQ KQLEKDICIT ISCKKTVKKL
610 620 630 640 650
SWHRKGDYFV TVQPDSGNTS VLIHQVSKHL TQSPFKKSKG IIMDAKFHPF
660 670 680 690 700
KPQLFVCSQR YVRIYDLSQQ ILVKKLLPGA RWLSKIDIHP RGDNLIASSF
710 720 730 740 750
DKRVLWHDLD LASTPYKTLR YHEKAVRSVN FHKKLPLFSS AADDGTIHVF
760 770 780 790 800
HATVYDDMMK NPMIVPLKKL TGHKVINSLG VLDAIWHPRE AWLFSAGADN

TARLWTT
Length:807
Mass (Da):91,705
Last modified:November 1, 1997 - v1
Checksum:i6542DFE4670341CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49703 Genomic DNA. Translation: CAA89759.1.
BK006946 Genomic DNA. Translation: DAA09948.1.
PIRiS54549.
RefSeqiNP_013764.1. NM_001182546.1.

Genome annotation databases

EnsemblFungiiYMR049C; YMR049C; YMR049C.
GeneIDi855068.
KEGGisce:YMR049C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49703 Genomic DNA. Translation: CAA89759.1.
BK006946 Genomic DNA. Translation: DAA09948.1.
PIRiS54549.
RefSeqiNP_013764.1. NM_001182546.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4U7AX-ray1.60A1-807[»]
ProteinModelPortaliQ04660.
SMRiQ04660.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35223. 126 interactors.
DIPiDIP-3970N.
IntActiQ04660. 67 interactors.
MINTiMINT-575265.

PTM databases

iPTMnetiQ04660.

Proteomic databases

MaxQBiQ04660.
PRIDEiQ04660.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR049C; YMR049C; YMR049C.
GeneIDi855068.
KEGGisce:YMR049C.

Organism-specific databases

EuPathDBiFungiDB:YMR049C.
SGDiS000004652. ERB1.

Phylogenomic databases

GeneTreeiENSGT00390000018422.
HOGENOMiHOG000029368.
InParanoidiQ04660.
KOiK14824.
OMAiPTIVPVK.
OrthoDBiEOG092C1381.

Enzyme and pathway databases

BioCyciYEAST:G3O-32754-MONOMER.

Miscellaneous databases

PROiQ04660.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
HAMAPiMF_03027. BOP1. 1 hit.
InterProiIPR028598. BOP1/Erb1.
IPR012953. BOP1_N_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08145. BOP1NT. 1 hit.
PF00400. WD40. 3 hits.
[Graphical view]
SMARTiSM01035. BOP1NT. 1 hit.
SM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiERB1_YEAST
AccessioniPrimary (citable) accession number: Q04660
Secondary accession number(s): D6VZM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2680 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.