Q04658 (SMA2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spore membrane assembly protein 2

Gene names

Name:	SMA2
Ordered Locus Names:	YML066C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in spore and ascus formation. Required for the efficient assembly of the precursors of the prospore membrane to a continuous prospore membrane. Ref.4 Ref.5 Ref.7
Subcellular location	Prospore membrane; Multi-pass membrane protein. Endoplasmic reticulum. Note: Localizes to prospore membrane, and accumulates in the endoplasmic reticulum in vegetative and sporulating ERV14-deleted cells. Ref.7
Sequence similarities	Belongs to the SMA2 family.

Ontologies

Keywords
Biological process	Sporulation
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ascospore wall assembly Inferred from mutant phenotype Ref.4. Source: SGD spore membrane bending pathway Inferred from mutant phenotype. Source: SGD
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW prospore membrane Inferred from direct assay Ref.7. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	Spore membrane assembly protein 2		PRO_0000203252
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 27	21	Helical; Potential
	Topological domain	28 – 220	193	Lumenal Potential
	Transmembrane	221 – 241	21	Helical; Potential
	Topological domain	242 – 265	24	Cytoplasmic Potential
	Transmembrane	266 – 286	21	Helical; Potential
	Topological domain	287 – 319	33	Lumenal Potential
	Transmembrane	320 – 340	21	Helical; Potential
	Topological domain	341 – 369	29	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	Q04658 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 37CA79238F4CCAEF	FASTA	369	40,872
	10 20 30 40 50 60 MLFPKRLIVW GVLLILSLSQ FVLYLPATTC TNSKGLRLCA PQFTITVIGG SSTANEFIAS 70 80 90 100 110 120 VREFLRLISY LTIDMGWSNE FTDPSVYEDE NLVDTFQPDK VFELNYFGFC KRSNKSKVYC 130 140 150 160 170 180 TSNENYGMDV LEVLVRDVGI QLGNISTTRS NETKKFGDSL VLTYRLALTS IRDFLKHDKH 190 200 210 220 230 240 TGNALSKALI GSPDPNVKGV SPTKNYLKGV NLAFILMMFN GMVFYFAVLE IIVGFLSICV 250 260 270 280 290 300 VSAFGGALSV GKRHRLFPML LKSSSSILVV IATLTILCNI VYLIALKTLE PEEVTDVGSD 310 320 330 340 350 360 NAAVHTTGWE LLKVNVGSGF IMGLARYAIQ WVLLVLAFLA ANHYKAKPKK SDKYTEDTSN SPSPDLMEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"A screen for genes required for meiosis and spore formation based on whole-genome expression." Rabitsch K.P., Toth A., Galova M., Schleiffer A., Schaffner G., Aigner E., Rupp C., Penkner A.M., Moreno-Borchart A.C., Primig M., Esposito R.E., Klein F., Knop M., Nasmyth K. Curr. Biol. 11:1001-1009(2001) [PubMed: 11470404] [Abstract] Cited for: FUNCTION.
[5]	"Parallel phenotypic analysis of sporulation and postgermination growth in Saccharomyces cerevisiae." Deutschbauer A.M., Williams R.M., Chu A.M., Davis R.W. Proc. Natl. Acad. Sci. U.S.A. 99:15530-15535(2002) [PubMed: 12432101] [Abstract] Cited for: FUNCTION.
[6]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[7]	"Erv14 family cargo receptors are necessary for ER exit during sporulation in Saccharomyces cerevisiae." Nakanishi H., Suda Y., Neiman A.M. J. Cell Sci. 120:908-916(2007) [PubMed: 17298976] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z38114 Genomic DNA. Translation: CAA86255.1. AY557993 Genomic DNA. Translation: AAS56319.1. BK006946 Genomic DNA. Translation: DAA09831.1.
PIR	S48332.
RefSeq	NP_013645.1. NM_001182425.1.
3D structure databases
ProteinModelPortal	Q04658.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q04658. 2 interactions.
STRING	Q04658.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML066C; YML066C; YML066C.
GeneID	854936.
KEGG	sce:YML066C.
NMPDR	fig|4932.3.peg.4682.
Organism-specific databases
CYGD	YML066c.
SGD	S000004531. SMA2.
Phylogenomic databases
eggNOG	fuNOG10997.
HOGENOM	HBG203543.
OMA	NYFGFCK.
OrthoDB	EOG4T4H4Q.
Gene expression databases
ArrayExpress	Q04658.
Genevestigator	Q04658.
GermOnline	YML066C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	977977.

Entry information

Entry name

SMA2_YEAST

Accession

Primary (citable) accession number: Q04658
Secondary accession number(s): D6VZA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	December 14, 2011
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program