Reviewed, UniProtKB/Swiss-Prot Q04657 (KATG_MYCIT)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase
      Short name=CP
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase
    Protein Mi85

Gene names

Name:	katG
Synonyms:	Mi85

Organism

Mycobacterium intracellulare

Taxonomic identifier

1767 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC)

Protein attributes

Sequence length	746 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. May play a role in the intracellular survival of mycobacteria.
Catalytic activity	2 H(2)O(2) = O(2) + 2 H(2)O. Donor + H(2)O(2) = oxidized donor + 2 H(2)O.
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 746	746	Catalase-peroxidase		PRO_0000055570
Sites
	Active site	114	1	Proton acceptor By similarity
	Metal binding	277	1	Iron (heme axial ligand) By similarity
	Site	110	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	113 ↔ 236		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-262) By similarity
	Cross-link	236 ↔ 262		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-113) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q04657-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 15F35F7F5028F2B2	FASTA	746	81,418
	10 20 30 40 50 60 MSSDTSSSRP PQPDSGTASK SESENPAIPS PKPKAHAPLT NRDWWPDQVD VSSLHPHSPL 70 80 90 100 110 120 SNPLGDDFDY AAEFAKLDVE ALKADMISLM TTSQDWWPAD YGHYGGLFIR MSWHAAGTYR 130 140 150 160 170 180 IHDGRGGAGQ GMQRFAPLNS WPDNASLDKA RRLLWPIKKK YGNKISWADL ITYAGNVALE 190 200 210 220 230 240 SMGFKTFGFG FGREDVWEPE EILWGEEEEW LGTDKRYSGE RELAQPYGAT TMGLIYVNPE 250 260 270 280 290 300 GPEGKPDPIA AAIDIRETFG RMAMNDEETA ALIVGGHSFG KTHGAGDADL VGPEPEAAPI 310 320 330 340 350 360 EQQGLGWKSS YGTGSGKDAI TSGLEVVWTP TPTKWDNSFL ETLYGYEWEL TKSPAGAWQF 370 380 390 400 410 420 TAKDGAGAGT IPDPFGGAGR APTMLVTDIS LRESPIYADI TRRWLDHPEE LADAFAKAWY 430 440 450 460 470 480 KLLHRDMGPI SRYLGPWVAE PQLWQDPVPA VDHELVDDND VAALKKKVLD SGLSIPQLVK 490 500 510 520 530 540 TAWSAAASYR NTDKRGGANG GRLRLQPQRS WEVNEPSELD KVLPVLEKIQ QDFNASASGG 550 560 570 580 590 600 KKISLADLIV LAGSAAVEKA AKDAGYEISV HFAPGRTDAS QESTDVESFA VLEPRADGFR 610 620 630 640 650 660 NYIRPGEKAP LEQLLIERAY LLGVTGPEMT VLVGGLRALG ANHGSSKHGV FTDRPGALTN 670 680 690 700 710 720 DFFVNLLDMG TEWKASETAE NVYEGRDRAS GALKWTATAN DLVFGSNSVL RGLVEVYAQD 730 740 DAHGKFVEDF VAAWVKVMNS DRFDLK

References

[1]	"The catalase-peroxidase of Mycobacterium intracellulare: nucleotide sequence analysis and expression in Escherichia coli." Morris S.L., Nair J., Rouse D.A. J. Gen. Microbiol. 138:2363-2370(1992) [PubMed: 1336034] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M86741 Genomic DNA. Translation: AAA25360.1.
PIR	A47685.
3D structure databases
HSSP	HSSP built from PDB template 1MWV based on UniProtKB Q939D2.
ModBase	Search...
Protein family/group databases
PeroxiBase	2433. MinCP01.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_MYCIT

Accession

Primary (citable) accession number: Q04657

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	November 25, 2008
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)