Catalase-peroxidase - Mycobacterium intracellulare

Preferred order of sections

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase
Protein Mi85

Gene names

Name:	katG
Synonyms:	Mi85

Organism

Mycobacterium intracellulare

Taxonomic identifier

1767 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC)

Protein attributes

Sequence length	746 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. May play a role in the intracellular survival of mycobacteria. HAMAP MF_01961
Catalytic activity	Donor + H₂O₂ = oxidized donor + 2 H₂O. HAMAP MF_01961 2 H₂O₂ = O₂ + 2 H₂O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 746

746

Catalase-peroxidase HAMAP MF_01961

PRO_0000055570

Sites

Active site

114

1

Proton acceptor By similarity

Metal binding

277

1

Iron (heme axial ligand) By similarity

Site

110

1

Transition state stabilizer By similarity

Amino acid modifications

Cross-link

113 ↔ 236

Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-262) By similarity

Cross-link

236 ↔ 262

Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-113) By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q04657 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 15F35F7F5028F2B2
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FASTA

746

81,418

        10         20         30         40         50         60 
MSSDTSSSRP PQPDSGTASK SESENPAIPS PKPKAHAPLT NRDWWPDQVD VSSLHPHSPL 

        70         80         90        100        110        120 
SNPLGDDFDY AAEFAKLDVE ALKADMISLM TTSQDWWPAD YGHYGGLFIR MSWHAAGTYR 

       130        140        150        160        170        180 
IHDGRGGAGQ GMQRFAPLNS WPDNASLDKA RRLLWPIKKK YGNKISWADL ITYAGNVALE 

       190        200        210        220        230        240 
SMGFKTFGFG FGREDVWEPE EILWGEEEEW LGTDKRYSGE RELAQPYGAT TMGLIYVNPE 

       250        260        270        280        290        300 
GPEGKPDPIA AAIDIRETFG RMAMNDEETA ALIVGGHSFG KTHGAGDADL VGPEPEAAPI 

       310        320        330        340        350        360 
EQQGLGWKSS YGTGSGKDAI TSGLEVVWTP TPTKWDNSFL ETLYGYEWEL TKSPAGAWQF 

       370        380        390        400        410        420 
TAKDGAGAGT IPDPFGGAGR APTMLVTDIS LRESPIYADI TRRWLDHPEE LADAFAKAWY 

       430        440        450        460        470        480 
KLLHRDMGPI SRYLGPWVAE PQLWQDPVPA VDHELVDDND VAALKKKVLD SGLSIPQLVK 

       490        500        510        520        530        540 
TAWSAAASYR NTDKRGGANG GRLRLQPQRS WEVNEPSELD KVLPVLEKIQ QDFNASASGG 

       550        560        570        580        590        600 
KKISLADLIV LAGSAAVEKA AKDAGYEISV HFAPGRTDAS QESTDVESFA VLEPRADGFR 

       610        620        630        640        650        660 
NYIRPGEKAP LEQLLIERAY LLGVTGPEMT VLVGGLRALG ANHGSSKHGV FTDRPGALTN 

       670        680        690        700        710        720 
DFFVNLLDMG TEWKASETAE NVYEGRDRAS GALKWTATAN DLVFGSNSVL RGLVEVYAQD 

       730        740 
DAHGKFVEDF VAAWVKVMNS DRFDLK

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References

[1]	"The catalase-peroxidase of Mycobacterium intracellulare: nucleotide sequence analysis and expression in Escherichia coli." Morris S.L., Nair J., Rouse D.A. J. Gen. Microbiol. 138:2363-2370(1992) [PubMed: 1336034] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M86741 Genomic DNA. Translation: AAA25360.1.
PIR	A47685.
3D structure databases
ProteinModelPortal	Q04657.
SMR	Q04657. Positions 41-746.
ModBase	Search...
Protein family/group databases
PeroxiBase	2433. MinCP01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_MYCIT

Accession

Primary (citable) accession number: Q04657

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	October 19, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families