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Protein

Translation termination inhibitor protein ITT1

Gene

ITT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Modulates the efficiency of translation termination, resulting in the readthrough of all three types of nonsense codons UAA, UAG and UGA.1 Publication

Miscellaneous

Present with 846 molecules/cell in log phase SD medium.1 Publication

Caution

Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family.Curated

Catalytic activityi

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei415By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri180 – 232RING-type 1PROSITE-ProRule annotationAdd BLAST53
Zinc fingeri272 – 338IBR-typeAdd BLAST67
Zinc fingeri402 – 431RING-type 2; degeneratePROSITE-ProRule annotationAdd BLAST30

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processTranslation regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32663-MONOMER
ReactomeiR-SCE-8866654 E3 ubiquitin ligases ubiquitinate target proteins
R-SCE-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Translation termination inhibitor protein ITT1 (EC:2.3.2.31By similarity)
Gene namesi
Name:ITT1
Ordered Locus Names:YML068W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML068W
SGDiS000004533 ITT1

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000563401 – 464Translation termination inhibitor protein ITT1Add BLAST464

Proteomic databases

MaxQBiQ04638
PaxDbiQ04638
PRIDEiQ04638

Interactioni

Subunit structurei

Interacts with translation release factors eRF1 (SUP45) and eRF3 (SUP35) in vitro.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SUP35P054533EBI-27858,EBI-6540

GO - Molecular functioni

Protein-protein interaction databases

BioGridi35098, 81 interactors
DIPiDIP-1952N
IntActiQ04638, 7 interactors
MINTiQ04638
STRINGi4932.YML068W

Structurei

3D structure databases

ProteinModelPortaliQ04638
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Members of the RBR family are atypical E3 ligases. They interact with the E2 conjugating enzyme UBE2L3 and function like HECT-type E3 enzymes: they bind E2s via the first RING domain, but require an obligate trans-thiolation step during the ubiquitin transfer, requiring a conserved cysteine residue in the second RING domain.By similarity

Sequence similaritiesi

Belongs to the RBR family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri180 – 232RING-type 1PROSITE-ProRule annotationAdd BLAST53
Zinc fingeri272 – 338IBR-typeAdd BLAST67
Zinc fingeri402 – 431RING-type 2; degeneratePROSITE-ProRule annotationAdd BLAST30

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00840000129738
HOGENOMiHOG000113118
InParanoidiQ04638
KOiK11971
OMAiFEGMPGT
OrthoDBiEOG092C1WVW

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR031127 E3_UB_ligase_RBR
IPR002867 IBR_dom
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR11685 PTHR11685, 1 hit
PfamiView protein in Pfam
PF01485 IBR, 2 hits
SMARTiView protein in SMART
SM00647 IBR, 2 hits
SM00184 RING, 2 hits
PROSITEiView protein in PROSITE
PS50089 ZF_RING_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q04638-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALTQFENDL EILRDMYPEL EMKSVKVEEE GEFPQRINGK LLFKISLLAD
60 70 80 90 100
VNIEFGEQHM LLSNLSNECV EFTIYSCHYP DIRRCVVMDI KSLWISTDEK
110 120 130 140 150
KMLIDKALRL VEETVDMSIE FADSFTSILI LIFGFLIDDT AILLFPNGIR
160 170 180 190 200
KCLTQDQYDL FKQISEEATL QKVSRSNYHC CICMEMEKGV RMIKLPCENA
210 220 230 240 250
NVEHYLCRGC AKSYFTAMIQ ENRISSVRCP QCEYKELKLE DFKSYKKMLK
260 270 280 290 300
ALFTPLIPVS FLKEVIDTEL CERYEKMFYN QAATRLSKYC PYACVTCRRC
310 320 330 340 350
DSWCTKEDLD DAMIQCQKCH FVFCFDCLHA WHGYNNKCGK KVSLSTDIIE
360 370 380 390 400
EYLDDTVTSY ERKRKLEAKY GRRVLELEVN DYLAEKMLDL AIKKEGSNLQ
410 420 430 440 450
RCPKCKVVVE RSEGCNKMKC EVCGTLFCFI CGVLLYPEDP YEHFREAYSG
460
CYGRLFEGMP GTET
Length:464
Mass (Da):54,096
Last modified:November 1, 1997 - v1
Checksum:iF481372B742AF63D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38114 Genomic DNA Translation: CAA86252.1
BK006946 Genomic DNA Translation: DAA09829.1
PIRiS48329
RefSeqiNP_013643.1, NM_001182427.1

Genome annotation databases

EnsemblFungiiYML068W; YML068W; YML068W
GeneIDi854934
KEGGisce:YML068W

Similar proteinsi

Entry informationi

Entry nameiITT1_YEAST
AccessioniPrimary (citable) accession number: Q04638
Secondary accession number(s): D6VZA5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names
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