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Protein

Translation termination inhibitor protein ITT1

Gene

ITT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Modulates the efficiency of translation termination, resulting in the readthrough of all three types of nonsense codons UAA, UAG and UGA.1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei415By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri180 – 232RING-type 1PROSITE-ProRule annotationAdd BLAST53
Zinc fingeri272 – 338IBR-typeAdd BLAST67
Zinc fingeri402 – 431RING-type 2; degeneratePROSITE-ProRule annotationAdd BLAST30

GO - Molecular functioni

GO - Biological processi

  • regulation of translational termination Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Translation regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32663-MONOMER.
ReactomeiR-SCE-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Translation termination inhibitor protein ITT1 (EC:2.3.2.-By similarity)
Gene namesi
Name:ITT1
Ordered Locus Names:YML068W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML068W.
SGDiS000004533. ITT1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000563401 – 464Translation termination inhibitor protein ITT1Add BLAST464

Proteomic databases

MaxQBiQ04638.
PRIDEiQ04638.

Interactioni

Subunit structurei

Interacts with translation release factors eRF1 (SUP45) and eRF3 (SUP35) in vitro.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SUP35P054533EBI-27858,EBI-6540

Protein-protein interaction databases

BioGridi35098. 39 interactors.
DIPiDIP-1952N.
IntActiQ04638. 4 interactors.
MINTiMINT-403947.

Structurei

3D structure databases

ProteinModelPortaliQ04638.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Members of the RBR family are atypical E3 ligases. They interact with the E2 conjugating enzyme UBE2L3 and function like HECT-type E3 enzymes: they bind E2s via the first RING domain, but require an obligate trans-thiolation step during the ubiquitin transfer, requiring a conserved cysteine residue in the second RING domain.By similarity

Sequence similaritiesi

Belongs to the RBR family.Curated
Contains 1 IBR-type zinc finger.Curated
Contains 2 RING-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri180 – 232RING-type 1PROSITE-ProRule annotationAdd BLAST53
Zinc fingeri272 – 338IBR-typeAdd BLAST67
Zinc fingeri402 – 431RING-type 2; degeneratePROSITE-ProRule annotationAdd BLAST30

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00840000129738.
HOGENOMiHOG000113118.
InParanoidiQ04638.
KOiK11971.
OMAiFEGMPGT.
OrthoDBiEOG092C1WVW.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR031127. E3_UB_ligase_RBR.
IPR002867. IBR_dom.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR11685. PTHR11685. 3 hits.
PfamiPF01485. IBR. 2 hits.
[Graphical view]
SMARTiSM00647. IBR. 2 hits.
SM00184. RING. 2 hits.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04638-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALTQFENDL EILRDMYPEL EMKSVKVEEE GEFPQRINGK LLFKISLLAD
60 70 80 90 100
VNIEFGEQHM LLSNLSNECV EFTIYSCHYP DIRRCVVMDI KSLWISTDEK
110 120 130 140 150
KMLIDKALRL VEETVDMSIE FADSFTSILI LIFGFLIDDT AILLFPNGIR
160 170 180 190 200
KCLTQDQYDL FKQISEEATL QKVSRSNYHC CICMEMEKGV RMIKLPCENA
210 220 230 240 250
NVEHYLCRGC AKSYFTAMIQ ENRISSVRCP QCEYKELKLE DFKSYKKMLK
260 270 280 290 300
ALFTPLIPVS FLKEVIDTEL CERYEKMFYN QAATRLSKYC PYACVTCRRC
310 320 330 340 350
DSWCTKEDLD DAMIQCQKCH FVFCFDCLHA WHGYNNKCGK KVSLSTDIIE
360 370 380 390 400
EYLDDTVTSY ERKRKLEAKY GRRVLELEVN DYLAEKMLDL AIKKEGSNLQ
410 420 430 440 450
RCPKCKVVVE RSEGCNKMKC EVCGTLFCFI CGVLLYPEDP YEHFREAYSG
460
CYGRLFEGMP GTET
Length:464
Mass (Da):54,096
Last modified:November 1, 1997 - v1
Checksum:iF481372B742AF63D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38114 Genomic DNA. Translation: CAA86252.1.
BK006946 Genomic DNA. Translation: DAA09829.1.
PIRiS48329.
RefSeqiNP_013643.1. NM_001182427.1.

Genome annotation databases

EnsemblFungiiYML068W; YML068W; YML068W.
GeneIDi854934.
KEGGisce:YML068W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38114 Genomic DNA. Translation: CAA86252.1.
BK006946 Genomic DNA. Translation: DAA09829.1.
PIRiS48329.
RefSeqiNP_013643.1. NM_001182427.1.

3D structure databases

ProteinModelPortaliQ04638.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35098. 39 interactors.
DIPiDIP-1952N.
IntActiQ04638. 4 interactors.
MINTiMINT-403947.

Proteomic databases

MaxQBiQ04638.
PRIDEiQ04638.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML068W; YML068W; YML068W.
GeneIDi854934.
KEGGisce:YML068W.

Organism-specific databases

EuPathDBiFungiDB:YML068W.
SGDiS000004533. ITT1.

Phylogenomic databases

GeneTreeiENSGT00840000129738.
HOGENOMiHOG000113118.
InParanoidiQ04638.
KOiK11971.
OMAiFEGMPGT.
OrthoDBiEOG092C1WVW.

Enzyme and pathway databases

BioCyciYEAST:G3O-32663-MONOMER.
ReactomeiR-SCE-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiQ04638.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR031127. E3_UB_ligase_RBR.
IPR002867. IBR_dom.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR11685. PTHR11685. 3 hits.
PfamiPF01485. IBR. 2 hits.
[Graphical view]
SMARTiSM00647. IBR. 2 hits.
SM00184. RING. 2 hits.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiITT1_YEAST
AccessioniPrimary (citable) accession number: Q04638
Secondary accession number(s): D6VZA5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 846 molecules/cell in log phase SD medium.1 Publication

Caution

Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.