Reviewed, UniProtKB/Swiss-Prot Q04638 (ITT1_YEAST)

Names and origin

Protein names

Recommended name:
    Translation termination inhibitor protein ITT1

Gene names

Name:	ITT1
Ordered Locus Names:	YML068W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Modulates the efficiency of translation termination, resulting in the readthrough of all three types of nonsense codons UAA, UAG and UGA. Ref.2
Subunit structure	Interacts with translation release factors eRF1 (SUP45) and eRF3 (SUP35) in vitro. Ref.2
Miscellaneous	Present with 846 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the RBR family. Contains 1 IBR-type zinc finger. Contains 2 RING-type zinc fingers.
Caution	Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family.

Ontologies

Keywords
Biological process	Translation regulation
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of translational termination Ref.2 Inferred from mutant phenotype. Source: SGD
Molecular function	protein binding Ref.2 Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P38800	1	EBI-27858,EBI-24597
SUP1	P12385	1	EBI-27858,EBI-6533
SUP35	P05453	1	EBI-27858,EBI-6540
VPS64	Q03944	1	EBI-27858,EBI-30418

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Translation termination inhibitor protein ITT1		PRO_0000056340
Regions
	Zinc finger	180 – 232	53	RING-type 1
	Zinc finger	272 – 338	67	IBR-type
	Zinc finger	402 – 431	30	RING-type 2; degenerate

Sequences

	Sequence		Length	Mass (Da)
	Q04638-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: F481372B742AF63D	FASTA	464	54,096
	10 20 30 40 50 60 MALTQFENDL EILRDMYPEL EMKSVKVEEE GEFPQRINGK LLFKISLLAD VNIEFGEQHM 70 80 90 100 110 120 LLSNLSNECV EFTIYSCHYP DIRRCVVMDI KSLWISTDEK KMLIDKALRL VEETVDMSIE 130 140 150 160 170 180 FADSFTSILI LIFGFLIDDT AILLFPNGIR KCLTQDQYDL FKQISEEATL QKVSRSNYHC 190 200 210 220 230 240 CICMEMEKGV RMIKLPCENA NVEHYLCRGC AKSYFTAMIQ ENRISSVRCP QCEYKELKLE 250 260 270 280 290 300 DFKSYKKMLK ALFTPLIPVS FLKEVIDTEL CERYEKMFYN QAATRLSKYC PYACVTCRRC 310 320 330 340 350 360 DSWCTKEDLD DAMIQCQKCH FVFCFDCLHA WHGYNNKCGK KVSLSTDIIE EYLDDTVTSY 370 380 390 400 410 420 ERKRKLEAKY GRRVLELEVN DYLAEKMLDL AIKKEGSNLQ RCPKCKVVVE RSEGCNKMKC 430 440 450 460 EVCGTLFCFI CGVLLYPEDP YEHFREAYSG CYGRLFEGMP GTET

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Itt1p, a novel protein inhibiting translation termination in Saccharomyces cerevisiae." Urakov V.N., Valouev I.A., Lewitin E.I., Paushkin S.V., Kosorukov V.S., Kushnirov V.V., Smirnov V.N., Ter-Avanesyan M.D. BMC Mol. Biol. 2:9-9(2001) [PubMed: 11570975] [Abstract] Cited for: FUNCTION, INTERACTION WITH SUP35 AND SUP45.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z38114 Genomic DNA. Translation: CAA86252.1.
PIR	S48329.
RefSeq	NP_013643.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1952N.
IntAct	Q04638. 5 interactions.
STRING	Q04638.
Genome annotation databases
Ensembl	YML068W; YML068W; YML068W; Saccharomyces cerevisiae. [Genome view]
GeneID	854934.
KEGG	sce:YML068W.
NMPDR	fig|4932.3.peg.4680.
Organism-specific databases
CYGD	YML068w.
SGD	S000004533. ITT1.
Phylogenomic databases
HOGENOM	Q04638.
OMA	ERSEGCN
OrthoDB	EOG9PZKQB
Gene expression databases
ArrayExpress	Q04638.
Genevestigator	Q04638.
GermOnline	YML068W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002867. Znf_C6HC. IPR001841. Znf_RING. [Graphical view]
Pfam	PF01485. IBR. 2 hits. [Graphical view]
SMART	SM00647. IBR. 2 hits. SM00184. RING. 2 hits. [Graphical view]
PROSITE	PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	977971.

Entry information

Entry name

ITT1_YEAST

Accession

Primary (citable) accession number: Q04638

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 24, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)