Q04632 (COG8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Conserved oligomeric Golgi complex subunit 8
Short name=COG complex subunit 8

Alternative name(s):
Component of oligomeric Golgi complex 8

Gene names

Name:	COG8
Synonyms:	DOR1
Ordered Locus Names:	YML071C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	607 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as component of the peripheral membrane COG complex that is involved in intra-Golgi protein trafficking. COG is located at the cis-Golgi, and regulates tethering of retrograde intra-Golgi vesicles and possibly a number of other membrane trafficking events. Ref.4
Subunit structure	Component of the conserved oligomeric Golgi (COG or Sec34/Sec35) complex which consists of eight different proteins COG1-COG8. Ref.4
Subcellular location	Golgi apparatus membrane; Peripheral membrane protein By similarity.
Miscellaneous	Present with 6440 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the COG8 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Golgi apparatus Membrane
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	CVT pathway Inferred from mutant phenotype. Source: SGD intra-Golgi vesicle-mediated transport Inferred from genetic interaction. Source: SGD
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell Golgi transport complex Inferred from physical interaction Ref.4. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
COG1	P53079	6	EBI-6035,EBI-4835
COG2	P53271	4	EBI-6035,EBI-16614
COG5	P53951	3	EBI-6035,EBI-4841
COG6	P53959	5	EBI-6035,EBI-4829
COG7	P53195	2	EBI-6035,EBI-4847

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 607	607	Conserved oligomeric Golgi complex subunit 8		PRO_0000213525
Amino acid modifications
	Modified residue	410	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q04632 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 57E34E2DE36664B6	FASTA	607	69,829
	10 20 30 40 50 60 MELILNSLIS DDLTEEQKRL SLDFLQDILQ SNTKDYESYF SSRAVPGSIT EDIAEIDAEL 70 80 90 100 110 120 SALDRKIRKT LLDNTSQIIG NILENDDRAQ LDDIAKSLEQ LWELDTNINK AADRNVTNDD 130 140 150 160 170 180 INNESVSIDD FLEDDKEDND TGRIMTTESN NLARKKKEDE FHKALSRLRN RISTKEDDKD 190 200 210 220 230 240 DIRSDTLVTV LENLDSITDL MELPFLARTC IRTGHYQEAV MLYTHTTSLR SRFPGSTIVD 250 260 270 280 290 300 EVCEKVLNEI STTMLSGLVK LLSTNVSVNS LKKILQYLNS IPPFDGKTNK SLLSVFLAMR 310 320 330 340 350 360 YKFITDEIAS YPLDVESSNE SLIEMMVKRK IEVLREHVYM SLNVFLKSFL YDTNDLEIPF 370 380 390 400 410 420 PEELESTVLR INGTNEEKEI EEKEKETKKE EYQKQDSVAN NEEDVTENKS IEDVQEEVQG 430 440 450 460 470 480 KVEGEDDGAE RKTENEIENE TVNKTEDKAE KEKEEEVNTK DNKAEKEEEE INKVEVTPEE 490 500 510 520 530 540 PSKSIDNKAE KEEEEINKVE VTPEEPSKKI RTSKRENKIP TNAVMLQFVD KCITYVLKDL 550 560 570 580 590 600 TRGLNSIKLS DSVCLQLVYC SFRLCDLNRN YHHLFLKKIN DTSLFTTEQL ARAIDKRAEL ASKYIYS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-113. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	Brown D., Bowman S., Barrell B.G., Rajandream M.A. Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 88-607. Strain: ATCC 204511 / S288c / AB972.
[4]	"The Sec34/35 Golgi transport complex is related to the exocyst, defining a family of complexes involved in multiple steps of membrane traffic." Whyte J.R., Munro S. Dev. Cell 1:527-537(2001) [PubMed: 11703943] [Abstract] Cited for: FUNCTION, SUBUNIT.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"The binary interacting network of the conserved oligomeric Golgi tethering complex." Loh E., Hong W. J. Biol. Chem. 279:24640-24648(2004) [PubMed: 15047703] [Abstract] Cited for: COMPOSITION OF THE COG COMPLEX, INTERACTION WITH COG3 AND COG4.
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-410, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z38114 Genomic DNA. Translation: CAA86249.1. Z46373 Genomic DNA. Translation: CAA86507.1. BK006946 Genomic DNA. Translation: DAA09826.1.
PIR	S48326.
RefSeq	NP_013640.1. NM_001182430.1.
3D structure databases
ProteinModelPortal	Q04632.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6749N.
IntAct	Q04632. 21 interactions.
MINT	MINT-644150.
STRING	Q04632.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML071C; YML071C; YML071C.
GeneID	854904.
KEGG	sce:YML071C.
NMPDR	fig|4932.3.peg.4677.
Organism-specific databases
CYGD	YML071c.
SGD	S000004536. COG8.
Phylogenomic databases
eggNOG	fuNOG12498.
HOGENOM	HBG203541.
OMA	VYCSFRL.
OrthoDB	EOG4BCHX3.
Gene expression databases
ArrayExpress	Q04632.
Genevestigator	Q04632.
GermOnline	YML071C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007255. Dor1. [Graphical view]
PANTHER	PTHR21311. Dor1. 1 hit.
Pfam	PF04124. Dor1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977888.

Entry information

Entry name

COG8_YEAST

Accession

Primary (citable) accession number: Q04632
Secondary accession number(s): D6VZA2, Q03641

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	December 14, 2011
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program